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21.
Lee SC Kim JH Jeong SM Kim DR Ha JU Nam KC Ahn DU 《Journal of agricultural and food chemistry》2003,51(15):4400-4403
After far-infrared (FIR) radiation onto rice hull, a methanolic extract was prepared for the determination of antioxidant ability. After 30 min of FIR treatment, the radical scavenging activity and total phenol contents of rice hull extracts increased from 47.74 to 79.63% and from 0.12 to 0.19 mM, respectively, compared to control. Inhibition of lipid peroxidation in extracts was also increased from 41.07 to 47.96%. According to the GC-MS analysis, more phenolic compounds (p-coumaric acid, 3-vinyl-1-oxybenzene, p-hydroxybenzaldehyde, vanillin, p-hydroxybenzoic acid, and 4,7-dihydroxyvanillic acid) were detected in FIR-irradiated rice hull extract. These results indicated that FIR radiation onto rice hull could liberate and activate covalently bound phenolic compounds that have antioxidant activities. 相似文献
22.
6[8'(Z)-pentadecenyl]salicylic acid, otherwise known as anacardic acid (C15:1), inhibited the linoleic acid peroxidation catalyzed by soybean lipoxygenase-1 (EC 1.13.11.12, type 1) with an IC50 of 6.8 microM. The inhibition of the enzyme by anacardic acid (C15:1) is a slow and reversible reaction without residual activity. The inhibition kinetics analyzed by Dixon plots indicates that anacardic acid (C15:1) is a competitive inhibitor and the inhibition constant, KI, was obtained as 2.8 microM. Although anacardic acid (C15:1) inhibited the linoleic acid peroxidation without being oxidized, 6[8'(Z),11'(Z)-pentadecadienyl]salicylic acid, otherwise known as anacardic acid (C15:2), was dioxygenated at low concentrations as a substrate. In addition, anacardic acid (C15:2) was also found to exhibit time-dependent inhibition of lipoxygenase-1. The alk(en)yl side chain of anacardic acids is essential to elicit the inhibitory activity. However, the hydrophobic interaction alone is not enough because cardanol (C15:1), which possesses the same side chain as anacardic acid (C15:1), acted neither as a substrate nor as an inhibitor. 相似文献
23.
为实现灌区测量水规范化、标准化和自动化提供参考依据,保证灌区有限水资源科学管理与合理配置,达到公平和高效的利用目的,针对宁夏灌区测量水技术的发展现状进行了综合阐述.总结宁夏目前所采用的量水方法和在生产实际应用中存在的问题,提出灌区量水技术的发展趋势,通过提高灌区测量水的技术装备和测试手段,旨在提高灌区测量水技术水平和精度的有效保证. 相似文献
24.
Gamblin SJ Haire LF Russell RJ Stevens DJ Xiao B Ha Y Vasisht N Steinhauer DA Daniels RS Elliot A Wiley DC Skehel JJ 《Science (New York, N.Y.)》2004,303(5665):1838-1842
The 1918 influenza pandemic resulted in about 20 million deaths. This enormous impact, coupled with renewed interest in emerging infections, makes characterization of the virus involved a priority. Receptor binding, the initial event in virus infection, is a major determinant of virus transmissibility that, for influenza viruses, is mediated by the hemagglutinin (HA) membrane glycoprotein. We have determined the crystal structures of the HA from the 1918 virus and two closely related HAs in complex with receptor analogs. They explain how the 1918 HA, while retaining receptor binding site amino acids characteristic of an avian precursor HA, is able to bind human receptors and how, as a consequence, the virus was able to spread in the human population. 相似文献
25.
Hohng S Zhou R Nahas MK Yu J Schulten K Lilley DM Ha T 《Science (New York, N.Y.)》2007,318(5848):279-283
Despite the recent advances in single-molecule manipulation techniques, purely mechanical approaches cannot detect subtle conformational changes in the biologically important regime of weak forces. We developed a hybrid scheme combining force and fluorescence that allowed us to examine the effect of subpiconewton forces on the nanometer scale motion of the Holliday junction (HJ) at 100-hertz bandwidth. The HJ is an exquisitely sensitive force sensor whose force response is amplified with an increase in its arm lengths, demonstrating a lever-arm effect at the nanometer-length scale. Mechanical interrogation of the HJ in three different directions helped elucidate the structures of the transient species populated during its conformational changes. This method of mapping two-dimensional reaction landscapes at low forces is readily applicable to other nucleic acid systems and their interactions with proteins and enzymes. 相似文献
26.
卤虫做为一种经济动物已广泛应用于水产养殖业中,但是做为一种遗传学教学的实验材料尚无人报道过。通过多次实验,本文作者提出了一种改进后的观察卤虫染色体形态和数目的实验方法。同时,作者还例举了各种事实表明卤虫是一种很好的遗传学教学的实验材料。 相似文献
27.
28.
试验设不同培养基、不同温度、不同pH值3种处理,研究其对T-黄平菇菌丝生长的影响。同时对生料袋栽T-黄平菇的出菇时间及产量进行了试验。结果表明,T-黄平菇菌丝在PDA培养基上生长最为适宜;在26℃~28℃条件下菌丝生长最佳;菌丝生长的最适PH值为6 ~8。袋栽结果表明,T-黄平菇耐高温、出菇早、子实体形美色艳,是较好的高温型平菇品种之一. 相似文献
29.
Myung-Hoo Kim Ji-Young Yang Santi Devi Upadhaya Hyun-Jun Lee Cheol-Heui Yun Jong K. Ha 《Journal of veterinary science (Suw?n-si, Korea)》2011,12(2):151-157
The purpose of our study was to investigate changes in immunological parameters induced by weaning stress (including milk restriction) in calves. Fifteen Holstein calves were subjected to weaning at 6 weeks of age. Blood samples were collected at -14, -7, -2, 1, 3, and 5 days post-weaning (DPW; 0 DPW = 42 days). Weaning caused significant (p < 0.01) increases in the neutrophil (NE):lymphocyte (LY) ratio at 5 DPW with a significant (p < 0.05) reduction of LYs. The concentration of acute-phase proteins (haptoglobin and serum amyloid A) also increased significantly (p < 0.05) at 3 and 5 DPW compared to -2 DPW. Levels of the iron-binding protein lactoferrin decreased significantly (p < 0.05) after weaning. Serum tumor necrosis factor-α and cortisol levels were elevated (p < 0.05) at 3 DPW, while those of serum interferon-γ decreased (p < 0.05) at 1 and 3 DPW compared to levels observed before weaning. Weaning significantly (p < 0.05) decreased the percentage of CD25+ T cells in the peripheral blood. In conclusion, weaning stress affected the NE:LY ratio along with the levels of acute phase proteins, lactoferrin, cortisol, and inflammatory cytokines in the peripheral blood of calves. Weaning stress may induce an acute phase response possibly through the elevation of cortisol production and modulation of inflammatory cytokines. 相似文献
30.
Lipoxygenase inhibitory activity of octyl gallate 总被引:2,自引:0,他引:2
Octyl gallate inhibited soybean lipoxygenase-1 (EC 1.13.11.12, type I) with an IC(50) of 1.3 microM. The inhibition of the enzyme by octyl gallate is a slow and reversible reaction without residual activity. The inhibition kinetics analyzed by Lineweaver-Burk plots indicates that octyl gallate is a competitive inhibitor, and the inhibition constant, K(I), was obtained as 0.54 microM. One molecule of octyl gallate scavenged six molecules of 1,1-diphenyl-2-picrylhydrazyl and inhibited autoxidative lipid peroxidation. In addition, octyl gallate was effective in preventing lipid peroxidation. 相似文献