Characteristics of Gelatin Extracted from the Swim Bladder of Yellowfin Tuna (Thunnus albacores) as Affected by Alkaline Pretreatments     

Onouma Kaewdang  Thummanoon Prodpran  Thammarat Kaewmanee  Hideki Kishimura 《Journal Of Aquatic Food Product Technology》2013,22(8):1190-1201

ABSTRACT

Gelatins extracted from the swim bladder of yellowfin tuna (Thunnus albacores) using various alkaline pretreatments were characterized. Alkaline mixtures (Na₂CO₃:NaOH) at different ratios (9:1, 8:2, 7:3, and 6:4) with a concentration of 4% (w/v) were used. The corresponding gelatins termed “G1,” “G2,” “G3,” and “G4” had yields of 9.78, 14.91, 35.96, and 13.60% (dry weight basis), respectively. All gelatins had α-chains as the major components. Fourier transform infrared spectra of obtained gelatins revealed the significant loss of molecular order of the triple-helix. G3 having the highest imino acid content and exhibited the highest gel strength (p < 0.05), compared with others. The microstructure of G3 gel was finer with smaller voids, compared with others. With increasing proportion of NaOH, the L*-value of gelatin gel increased with coincidental decrease in ΔE*-value. Gelling and melting temperatures of swim bladder gelatin were 12.3–15.1 and 21.3–22.3°C, respectively.  相似文献   

Hen egg yolk and skinned krill as possible foods for rearing leptocephalus larvae of Anguilla japonica Temminck & Schlegel     

Akihiro Okamura  Yoshiaki Yamada  Noriyuki Horie  Naomi Mikawa  Satoru Tanaka  Hideki Kobayashi  Katsumi Tsukamoto 《Aquaculture Research》2013,44(10):1531-1538

Usual diets for rearing leptocephalus larvae of Japanese eel Anguilla japonica include eggs of the endangered spiny dogfish Squalus acanthias (SE). We investigated the effects of alternative food materials, hen egg yolk (HEY) and exoskeleton‐free (skinned) Antarctic krill (SAK), on the growth and survival of eel larvae. We found that feed comprising whole krill including exoskeleton (WAK) containing higher levels of fluoride (37.89 mg kg^?1) was acutely toxic to eel larvae exposed to this alone. In contrast, extract from SAK containing lower concentrations of fluoride (4.25 mg kg^?1) showed no apparent adverse effects. Growth of larvae fed a mixture of SE and SAK in a feed trial of 58 days [mean body weight (BW), 6.0 mg] was about twofold higher than that of larvae fed a mixture of SE and WAK (3.2 mg) (P < 0.01). A mixture of HEY and SAK also had some dietary benefits for eel larvae, enabling them to survive for up to 58 days and to grow significantly (mean BW, 2.4 mg), compared with their initial weight (mean BW, 0.2 mg) (P < 0.001). Although additional nutritional improvements are needed, the present results suggest that combination diet HEY and SAK may be a good alternative to SE as an effective diet for eel larvae.  相似文献   

Distribution of adipocyte-related cells in skeletal muscle of rainbow trout Oncorhynchus mykiss     

Yuna Han  Gen Kaneko  Reiko Nagasaka  Hidehiro Kondo  Ikuo Hirono  Shin-Ichiro Takahashi  Shugo Watabe  Hideki Ushio 《Fisheries Science》2013,79(1):143-148

Adiponectin is one of the adipokines secreted mainly from adipocytes in mammals. In rainbow trout, however, adiponectin is highly expressed in skeletal muscle. Although it has been suggested that fish skeletal muscle contains adipocytes, their endocrine function and distribution are poorly understood. Recently, an EST analysis of rainbow trout found that heart-type fatty acid binding protein (H-FABP), a member of the intracellular fatty acid binding protein family, encodes an adipose-specific gene. In this study, we produced anti-adiponectin and H-FABP antibodies and investigated the distribution of adipocytes and related cells in skeletal muscle of rainbow trout. The adiponectin signal was detected at around 75 kDa in muscle in Western blotting. Since the molecular mass of rainbow trout adiponectin is around 25 kDa, this 75 kDa band would be a trimer. For H-FABP, the signal band was detected at around 15 kDa. Immunohistochemistry of skeletal muscle sections indicated that adiponectin and H-FABP signals were present outside of muscle cells and throughout the muscle tissues, suggesting the existence of adipocyte-related cells in these regions. These results will contribute to our understanding of energy metabolism in fish skeletal muscle.  相似文献   

Thermal gelation properties of white croaker, walleye pollack and deepsea bonefish surimi after suwari treatment at various temperatures     

Yoko Matsuoka  Jianrong Wan  Hideki Ushio  Shugo Watabe 《Fisheries Science》2013,79(4):715-724

The effects of setting (suwari) at around 40 °C on the breaking strength and breaking strain rate of thermal gels treated at 85 °C for 20 min during the following processing step were examined in association with the polymerization and degradation of myosin heavy chains (MHCs) for surimi prepared from white croaker, walleye pollack and deepsea bonefish. In the case of white croaker and walleye pollack, maximum values of breaking strength and breaking strain rate were obtained after suwari at 30–40 °C for both 30 and 60 min, at which temperature MHCs were polymerized. In comparison, these textual properties of the thermal gels decreased in surimi prepared from deepsea bonefish after suwari at around 38 °C for 30 min and at around 32 °C for 60 min, with concomitant degradation products of MHC. The textual properties of deepsea bonefish after suwari at temperatures >45 °C tended to be almost the same as those after suwari at temperatures of <30 °C, where neither polymerization nor degradation of MHC was observed.  相似文献   

Recent studies on biological control of plant diseases in Japan     

Mitsuro Hyakumachi  Hideki Takahashi  Yoichi Matsubara  Nobutaka Someya  Masafumi Shimizu  Kappei Kobayashi  Masamichi Nishiguchi 《Journal of General Plant Pathology》2014,80(4):287-302

Microorganisms play an enormously important role in plant disease control. Research on biological control of plant pathogens has received major impetus and attracted many researchers during the past few decades due to the increased public concern on hazards associated with the use of synthetic pesticides. From research on utilizing specific antagonistic microorganisms, many effective biological control agents (BCAs) have been found and are increasingly implemented in integrated pest management strategies to control plant diseases. Here current research results on biological control against plant diseases carried out in Japan are reviewed by focusing on major categories of BCAs: fungi, bacteria and actinomycetes and attenuated viruses.  相似文献   

Isolation and characterization of bacteria causing mortality in early stage larvae of captive‐bred Japanese eels (Anguilla japonica Temminck & Schlegel)          下载免费PDF全文

Gentoku Nakase  Masaharu Tokuda  Kazuharu Nomura  Hideki Tanaka 《Aquaculture Research》2015,46(11):2637-2643

Bacteria with lethal effect on eel larvae were isolated from moribund captive‐bred eel larvae and their 16S rRNA gene sequences were analysed. Nucleotide sequence of 10 isolates showed highest similarity with Lacinutrix algicola, Crocinitomix catalasitica and Pseudoalteromonas rubra. Age‐dependent changes in the susceptibility of eel larvae were observed in response to challenge by a highly lethal isolate. Compared with 10 and 11 days after hatching (DAH), larval susceptibility to the highly lethal isolate was lower at 18 DAH. We found that the bacterial isolates have lethal effect on the captive‐bred eel larvae, especially at the early developmental stage. These results will be useful to establish appropriate culture practices for eel larvae that will improve the success of mass production of glass eels for aquaculture.  相似文献   

Fish to 2030: The Role and Opportunity for Aquaculture     

Mimako Kobayashi  Siwa Msangi  Miroslav Batka  Stefania Vannuccini  Madan M. Dey  James L. Anderson 《Aquaculture Economics & Management (Blackwell Science)》2015,19(3):282-300

Seafood sector can contribute to the global food supply in an important way, and provide an important source of animal protein. Based on observed regional trends in seafood production and consumption and using a global, partial-equilibrium, multi-market model, this study investigates what the global seafood market may look like in 2030. The model projects that the total fish supply will increase from 154 million tons in 2011 to 186 million tons in 2030, with aquaculture entirely responsible for the increase. The fastest aquaculture growth is expected for tilapia and shrimp, while the largest expansion is expected in India, Latin America and Caribbean and Southeast Asia. Fast-growing seafood demand in China and elsewhere represents a critical opportunity for global fisheries and aquaculture to improve their management and achieve sustainable seafood economy.  相似文献   

Life cycle and control of Colletotrichum nymphaeae,the causal agent of celery stunt anthracnose     

Naho Yamagishi  Masashi Fujinaga  Yoshiyuki Ishiyama  Hideki Ogiso  Toyozo Sato  Yukio Tosa 《Journal of General Plant Pathology》2015,81(4):279-286

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Overexpression of <Emphasis Type="Italic">OsHAP2E</Emphasis> for a CCAAT-binding factor confers resistance to <Emphasis Type="Italic">Cucumber mosaic virus</Emphasis> and <Emphasis Type="Italic">Rice necrosis mosaic virus</Emphasis>     

Md.?Mahfuz?Alam  Hidemitsu?Nakamura  Hiroaki?Ichikawa  Kappei?Kobayashi  Takashi?Yaeno  Naoto?Yamaoka  Masamichi?NishiguchiEmail author 《Journal of General Plant Pathology》2015,81(1):32-41

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Effects of dietary microalgal species and hormone treatment on the lorica size and reproductivity of heavy-ion beam irradiated rotifers     

Kim  Hee-Jin  Nakamura  Kaichi  Sakakura  Yoshitaka  Suga  Koushirou  Tsuneizumi  Kazuhide  Abe  Tomoko  Yamada  Mieko  Kawada  Miki  Katayama  Takashi  Tezuka  Nobuhiro  Kobayashi  Takanori  Koiso  Masahiko  Hagiwara  Atsushi 《Fisheries Science》2023,89(1):61-69

In aquatic seeding production, the feeding regime of fish larvae consists of rotifers to Artemia, while there is a lack of intermediate-sized feed in the range of 350–600 µm. To solve this problem, in our previous study, the euryhaline rotifer Brachionus plicatilis (L-type, Notojima strain) were irradiated with carbon heavy-ion-beams, and large-sized rotifer mutant lines (>?350 µm lorica length) were established. This study aimed to enhance the mutated morphometric characteristics and reproductivity through the combined use of three microalgal species (Nannochloropsis oculata, Tetraselmis tetrathele, and Chlorella vulgaris), and two hormones [juvenile hormone (JH) and gamma-aminobutyric acid (GABA)]. The combination of T. tetrathele and JH was effective in enhancing the mutated phenotype, and the largest lorica length of 344–425 µm was observed with no significant differences in population growth between the wild-type and mutant strains. The combination of N. oculata and GABA enhanced the population growth of the mutant line, and its growth rate was 2.4 times higher than that of the control group (without GABA). This study indicates that the morphometric characteristics and reproductivity of rotifer mutant lines could be regulated by feed and hormone supplementation. These findings can promote the efficient use of mutant rotifer lines in aquaculture facilities.

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