马铃薯AP2/ERF转录因子的克隆及植物表达载体构建     

王慧  刘洪  杨奕琦  许思思  熊兴耀  周倩 《分子植物育种》2020,(8):2563-2568

AP2/ERF基因家族转录因子普遍存在于植物中,参与植物体内的各种生物学过程,包括植物的生长发育、生物和非生物胁迫响应等。前期转录组测序的结果发现马铃薯‘加湘1号’一个AP2/ERF家族基因(PGSC0003DMG400012154)在接种晚疫病菌(Phytophthora infestans)24 h后被显著激活。从接种P.infestans 24 h的‘加湘1号’的总RNA中通过RT-PCR获得了该基因CDS序列为885 bp,BLAST分析显示该基因编码一个295个氨基酸残基的蛋白,并且含有1个AP2/ERF结构域,是AP2/ERF转录因子家族中的ERF亚家族的一员。本研究将该基因与XcmⅠ酶切的表达载体pCXSN连接,转化大肠杆菌,通过测序挑选插入正确克隆酶切验证,并成功转化农杆菌。本研究结果为进一步研究该基因的功能提供了帮助。  相似文献   

Three dimensional (3D) spatial metrics for objects     

Kedron  Peter  Zhao  Yun  Frazier  Amy E. 《Landscape Ecology》2019,34(9):2123-2132

Landscape Ecology - The increasing availability of lidar data and structure from motion processing techniques is moving pattern metric research toward the development of three-dimensional (3D)...  相似文献   

Inhibition of postharvest senescence of green leafy vegetables by exogenous D-cysteine and L-cysteine as precursors of hydrogen sulphide     

H. M. S. Al Ubeed  M. C. Bowyer  J.B. Golding 《The Journal of Horticultural Science and Biotechnology》2019,94(5):620-626

Hydrogen sulphide (H₂S) inhibits senescence in harvested fruit and vegetables but presents logistical, safety and regulatory issues to become a commercial treatment. D-cysteine and L-cysteine are semi-essential amino acids that are metabolised to hydrogen sulphide by plant tissues albeit by different pathways. This paper examines the effect of cysteine on postharvest senescence of three green leafy vegetables. Spraying pak choy leaves with 10 mmol D-cysteine, L-cysteine or DL-cysteine inhibited leaf senescence through a delayed loss of green colour expressed as market life, reduced respiration rate and reduced ethylene production. The beneficial effects of cysteine were similar to those achieved by fumigation with hydrogen sulphide. L-cysteine sprays on parsley and peppermint leaves also showed reduced leaf colour loss and respiration compared to untreated leaves. Cysteine, either as the racemate or individual enantiomers, is considered to have commercial potential for green leafy vegetables as it provides the beneficial effect of hydrogen sulphide but should be easier to register for commercial use due to the GRAS status of L-cysteine.  相似文献   

NBA sweet spots: Distance‐based impacts on establishment‐level sales     

Brandli Stitzel  Cynthia L. Rogers 《Growth and change》2019,50(1):335-351

This paper examines distance‐based effects of the introduction of a National Basketball Association (NBA) team on establishment‐level sales. Using a unique micro dataset with precise geographic location information and industry detail, we apply spatio‐temporal estimation strategies following Harger et al. and Ahlfeldt and Kavetsos. We build on the literature by focusing on sales activity, a broad measure of economic activity, for industries related to the NBA‐product. Our application considers the relocation of the NBA’s Seattle franchise (Supersonics) to Oklahoma City (Thunder). The results reveal spatially differentiated impacts that would be obscured using data aggregated over space or industry. Specifically, food establishments exhibit increased sales and entertainment establishments exhibit decreased sales relative to establishments in the outer most ring of the study area.  相似文献   

Impact of land‐use intensity on the relationships between vegetation indices,photosynthesis and biomass of intensively and extensively managed grassland fens          下载免费PDF全文

C. M. H. Metzger  J. Heinichen  T. Eickenscheidt  M. Drösler 《Grass and Forage Science》2017,72(1):50-63

Vegetation indices are widely used as model inputs and for non‐destructive estimation of biomass and photosynthesis, but there have been few validation studies of the underlying relationships. To test their applicability on temperate fens and the impact of management intensity, we investigated the relationships between normalized difference vegetation index (NDVI), leaf area index (LAI), brown and green above‐ground biomass and photosynthesis potential (PP). Only the linear relationship between NDVI and PP was management independent (R² = 0·53). LAI to PP was described by a site‐specific and negative logarithmic function (R² = 0·07–0·68). The hyperbolic relationship of LAI versus NDVI showed a high residual standard error (s.e.) of 1·71–1·84 and differed between extensive and intensive meadows. Biomass and LAI correlated poorly (R² = 0·30), with high species‐specific variability. Intensive meadows had a higher ratio of LAI to biomass than extensive grasslands. The fraction of green to total biomass versus NDVI showed considerable noise (s.e. = 0·13). These relationships were relatively weak compared with results from other ecosystems. A likely explanation could be the high amount of standing litter, which was unevenly distributed within the vegetation canopy depending on the season and on the timing of cutting events. Our results show there is high uncertainty in the application of the relationships on temperate fen meadows. For reliable estimations, management intensity needs to be taken into account and several direct measurements throughout the year are required for site‐specific correction of the relationships, especially under extensive management. Using NDVI instead of LAI could reduce uncertainty in photosynthesis models.  相似文献   

贵州省食用菌产业现状与可持续性发展分析     

李银凤  刘晓柱 《中国农学通报》2020,36(16):160-164

笔者分析了贵州省食用菌产业特点与产业优势,认为贵州食用菌产业在自然条件、交通条件以及政策条件等方面均具有较大优势;全省食用菌规模逐渐扩大,品牌效应凸显。进一步总结了贵州省食用菌产业存在的问题,即缺乏行业规范,产品结构较为单一,行业人才匮乏。继而提出了贵州省食用菌产业可持续性发展建议,即加大食用菌产业的开发程度,加强科技支撑及专业人才培养,政府统筹,建立行业标准,重视菌渣的综合开发利用,以实现本省食用菌产业的后发赶超和可持续性发展。  相似文献   

Herbage production,nutritive value and animal productivity within hardwood silvopasture,open and mixed pasture systems in Appalachia,United States          下载免费PDF全文

J. P. S. Neel  D. P. Belesky 《Grass and Forage Science》2017,72(1):137-153

Demand for livestock food products is projected to increase dramatically through to 2050. Increased livestock production capacity on marginal lands will be critical to meeting this demand. A 5‐year research effort was undertaken to evaluate lamb and sward productivity within open and hardwood silvopasture (SP) systems in Appalachia, USA. Grazing began in mid to late April each year, with the grazing season averaging 141 d. Grazing system treatments during 2002 and 2003 grazing seasons were as follows: 100% open pasture (OP), 67% OP and 33% SP, and 67% OP and 33% SP with delayed SP grazing initiation (OSD). In 2004, a 100% SP (SP) system was added. Animals were rotationally stocked through either 6 (2002–2004) or 7 (2005–2006) paddocks. Open pasture produced greater (P < 0·001) grazing season herbage yield, while all systems generated similar animal performance. Based on summer solstice, herbage production in spring was greater (P < 0·001) than summer, except in 2003. Total non‐structural carbohydrate (TNC) content was greater (P < 0·05) in spring than in summer, except in 2004. Animal performance was superior in spring versus summer (P < 0·001). Animal plasma urea nitrogen (PUN) was lower (P < 0·05) for OP in 2003. When PUN was correlated with nutritive value indicators, the ratio of TNC to crude protein (CP) had the strongest correlation. The strong correlation indicates the need for synchronized ruminal energy and CP availability. Development of silvopasture from existing woodlots has potential to improve whole farm productivity on marginal lands.  相似文献   

Milk vetch dwarf virus infection in the Solanaceae and Caricaceae families in Southeast Asia     

Aamir Lal  Eui-Joon Kil  Vo T. B. Thuy  Chairina Fadhila  Phuong T. Ho  Hee-Seong Byun  Hang T. Dao  Ji-Kwang Kim  Sukchan Lee 《Plant pathology》2020,69(6):1026-1033

Milk vetch dwarf virus (MDV) is an important member of the genus Nanovirus and is transmitted by the aphid Aphis craccivora. MDV has multiple single-stranded DNA genome components, each approximately 1 kb, and two or three alpha-satellite molecules. It mainly infects plants of the legume family Fabaceae. Recently, papaya (Carica papaya) collected in Yesan, South Korea, displaying symptoms of leaf yellowing and dwarfism, was identified as a new host for MDV. To examine the geographical distribution of MDV, papaya samples with symptoms were harvested in South Korea, Vietnam, and Taiwan in August 2018, along with tomato and pepper samples grown in adjacent fields in Vietnam. The results revealed the presence of MDV not only in papaya but also in pepper and tomato. This MDV infection in members of the Solanaceae family was confirmed by Southern blot hybridization performed using a PCR product of segment S as a probe. Based on sequence analysis of three MDV components (M, S, and C3), we verified the presence of three different isolates of MDV in these three countries and homology between sequences of isolates from papaya and from members of the Solanaceae from Vietnam. Taken together, our results clearly demonstrate MDV infection in Vietnam and Taiwan for the first time and confirm that MDV can infect economically important pepper and tomato.  相似文献   

Atypical thymoma in a horse: Diagnostic approach and application of an alternative histological classification system     

T. Pudert  A. Weyrich  F. Geburek  C. Herden  M. Röcken 《Equine Veterinary Education》2021,33(11):e386-e392

Thymoma is a rare condition in horses. This case of a 24-year-old Warmblood gelding that was presented with oedema and signs of vascular congestion of the left forelimb caused by a thoracic mass is described. The diagnostic work-up included blood chemistry, cytology of pleural effusion, ultrasonography, radiography and the visualisation and collection of a tissue sample by thoracoscopy. Finally, post-mortem findings and histopathology revealed thymic epithelial neoplasia with histomorphologic features previously unreported in horses. The unique mixture of spindle-shaped and epithelioid tumour cells in combination with clear features of malignancy suggested the application of the Moran and Suster histological classification system for thymomas in humans (Moran and Suster, 2008, Curr. Treat. Options Oncol., 9, 288), which has not been reported in an equine case before and fits well to the present case.  相似文献   

优质、高产转基因抗虫棉杂交种苏杂668的选育与栽培技术   总被引：1，自引：1，他引：0  

肖松华  刘剑光  赵君  吴巧娟  徐剑文 《中国棉花》2017,44(10):17-18

本文概述了苏杂668的选育过程，阐明了苏杂668的特征特性、产量水平、纤维品质和抗性表现，并提出其高产栽培技术措施。  相似文献