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41.
Asadi Z. Mohammadi M. H. Shorafa M. Farhbakhsh M. Ghezelbash E. 《Eurasian Soil Science》2020,53(6):749-759
Eurasian Soil Science - The objectives of this research are prediction of soil drainage curve using Assouline and Or (2014) adjusted soil drainage model and comparison of the predicted results with... 相似文献
42.
Background: Phenylalanine dehydrogenase (PheDH; EC 1.4.1.20) is a NAD+-dependent enzyme that performs the reversible oxidative deamination of L-phenylalanine to phenylpyruvate. It plays an important role in detection and screening of phenylketonuria (PKU) diseases and production of chiral intermediates as well. The main goal of this study was to find a simple and rapid alternative method for purifying PheDH. Methods: The purification of recombinant Bacillus sphaericus PheDH was investigated in polyethylene glycol (PEG) and ammonium sulfate aqueous two-phase systems (ATPS). The influences of system parameters including PEG molecular weight and concentration, pH and (NH4)2SO4 concentration on enzyme partitioning were also studied. The purity of enzyme was analyzed by sodium dodecyl sulfate polyacrylamide gel electrophoresis. Results: A single extraction process was developed for separation and purification of recombinant PheDH from E. coli BL21 (DE3). The optimized conditions for partitioning and purification of PheDH were 9% (w/w) PEG-6,000 and 16% (w/w) (NH4)2SO4 at pH 8.0. The partition coefficient, recovery, yield, purification factor and specific activity values were achieved 58.7, 135%, 94.42%, 491.93 and 9828.88 U/mg, respectively. Also, the Km values for L-phenylalanine and NAD+ in oxidative deamination were 0.21 and 0.13 mM, respectively. Conclusion: The data presented in this paper demonstrated the potential of ATPS as a versatile and scaleable process for downstream processing of recombinant PheDH. 相似文献
43.
Kambiz Baghalian Ali Haghiry Mohammad Reza Naghavi Abodollah Mohammadi 《Scientia Horticulturae》2008,116(4):437-441
In arid and semi-arid regions, where water availability is a major limitation in crop production, using alternative water resources, such as saline water is one way to utilize lands. Chamomile (Matricaria recutita L.) as an annual medicinal herb may be considered as an economic substitute for field crops irrigated with fresh water since it has adaptability to wide range of climate and soil. A field examination was conducted during 2004–2005 using complete randomized block design with four replications in order to evaluate the effects of saline irrigation water on morphological characters, mineral content, oil quantity (content, yield), oil composition and apigenin content of chamomile. In each plot, 0.6 g/m2 of seeds were grown in 4 rows. The irrigation water had five different salinity levels (0, 4, 8, 12 and 16 dS m−1). The investigated characters through cultivation were fresh weight of flower (g), dry weight of flower (g), dry weight of aerial stems (g), dry weight of root (g), oil yield (kg/h), oil content (%), oil quality and apigenin content (%). After harvesting, the content of minerals (Na+, Cl−, K+, Ca2+, Mg2+) were evaluated in aerial parts and roots of each plot. Mean comparisons for fresh flower weight in different treatments showed that fresh flower yield decreased with increasing salinity and it was higher in control compared to others. Analysis of variance showed that saline irrigation water had no significant effect on oil quantity (yield and content), oil quality (chemical composition) or apigenin content. Our results showed that chamomile is able to maintain all its medical properties, under saline condition and could be cultivated economically in such conditions. 相似文献
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Genetic parameters for birth weight (BW), weaning weight (WW) and pre-weaning daily gain (PWDG) in Iranian Mehraban sheep were estimated using restricted maximum likelihood (REML) procedure. Six different animal models were fitted, differentiated by including or excluding maternal effects, with and without covariance between maternal and direct genetic effects. The estimates for direct heritability ranged from 0.26 to 0.53, 0.18 to 0.32 and 0.15 to 0.33 for BW, WW and PWDG respectively. The estimates were substantially higher when maternal effects, either genetic or environmental, were ignored in the model. The results of this study show that full models with maternal genetic and environmental effects gave the most accurate estimates for early growth traits. 相似文献
46.
Jahangir A Watanabe Y Chinen O Yamazaki S Sakai K Okamura M Nakamura M Takehara K 《Avian diseases》2008,52(1):49-53
Among winter migratory waterfowl, Northern pintails (Anas acuta), in one of the largest flocks in Tohoku district, northeast Japan, were surveyed for influenza A viruses at five wintering sites in three prefectures, viz., Aomori, Akita, and Miyagi. A total of 38 influenza A viruses were isolated from 2066 fecal samples collected during November 2006 through March 2007. The overall isolation rate was 1.84%. Eleven different subtypes were isolated, including nine H5N2, seven H6N8, seven H10N1, four H4N6, three H6N1, three H11N9, and one each of H1N1, H6N2, H6N5, H10N9, H11N1. Only the H4N6 subtype was detected during two successive months, November and December, from Lake Ogawara of Aomori prefecture. One wintering site, Lake Izunuma of Miyagi prefecture, was negative for virus isolation throughout the study period. During the sampling period, the highest virus isolation rate was in December (4.90%) followed by November (2.18%), January (0.91%), and February (0.30%). Virus isolation was negative for samples collected in March 2007. These results suggest that influenza viruses are introduced by Northern pintail when they migrate into Japan, but the viruses are not maintained in the flocks, most likely because the birds are not breeding during the winter. We believe that this relatively large data set creates a strong foundation for future studies of avian influenza virus (AIV) prevalence, evolution, and ecology in wintering sites, along with the role of Northern pintails in the spread of AIV during their migration from northern Russia and Asia to Japan. 相似文献
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Mojtaba Mohammadi Arani Amir Parviz Salati Omid Safari Saeed Keyvanshokooh 《Aquaculture Nutrition》2019,25(4):854-861
This study was carried out to assess the effects of Pediococcus acidilactici on zebrafish (Danio rerio). Different levels of P. acidilactici including 0, 1 × 106, 2 × 106, 4 × 106 and 8 × 106 colony‐forming unit per g of the diet (cfug?1) were examined in fish with 120 ± 10 mg weight for 60 days in a completely randomized design. The results showed that the best growth indices were recorded in group 4 × 106 cfug?1 (p < 0.05). The highest number of total viable count and lactic acid bacteria of intestine were found in group 4 × 106 cfug?1 (p < 0.05). The maximum activity of digestive enzymes including amylase, lipase, protease and alkaline phosphatase was observed in group 4 × 106 cfug?1. The highest activity for superoxide dismutase was recorded in group 4 × 106 cfug?1 while catalase, glutathione peroxidase and glutathione reductase showed the highest activity in group 8 × 106 cfug?1. The most growth inhibition zone of Aeromonas hydrophylla, Flavobacterium columnare, Vibrio anguillarum and Edwardsiella tarda was found in group 4 × 106 cfug‐1 (p < 0.05). Therefore, P. acidilactici as a probiotic improved growth and immunity of the zebrafish and could be used by zebrafish farmers. 相似文献
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Ghasem Mohammadi Taida Juliana Adorian Gholamreza Rafiee 《Aquaculture Nutrition》2020,26(5):1476-1492
The present research explored the effects of Bacillus subtilis on water quality, growth, immune responses, endotoxemia and protection against lipopolysaccharide (LPS) damages in Nile tilapia Oreochromis niloticus under biofloc system. B. subtilis was added at 0, 1, 2, 3 and 4 grams (1.19 × 108 CFU/g) per kg of basal diet, named T1 (control), T2, T3, T4 and T5, respectively, and fed to fish (14.82 ± 0.42 g) for 50 days. The concentrations of TAN, NO2 and NO3 were significantly reduced, and fish fed probiotics displayed significantly better growth performances versus the control, concomitantly with significantly enhanced activities of digestive enzymes. They also showed significantly declined serum glucose and cholesterol vice versa significantly improved immune responses (total protein, albumin, globulin, lysozyme, alternative complement, protease, immunoglobulins, alkaline phosphatase and respiratory burst), antioxidant capacity (superoxide dismutase, total antioxidant capacity, malondialdehyde) and skin mucus parameters (total protein, lysozyme, alternative complement, protease, immunoglobulins). Meanwhile, significantly lower endotoxin (LPS) concentrations were detected in the intestines and serum of fish fed probiotics. LPS challenge induced profound oxidative stress and impaired immune responses. Interestingly, probiotic alleviated LPS‐induced damages and restored mentioned parameters. In conclusion, B. subtilis effectively enhanced fish production, immunity and protection against LPS‐induced damages in tilapia under biofloc system. 相似文献
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Asad Mohammadi Zarejabad Mohammad Ali Jalali Mohammad Sudagar Somayeh Pouralimotlagh 《Fish physiology and biochemistry》2010,36(3):655-659
The effect of environmental salinity on hematological parameters of great sturgeon Huso huso juveniles was studied. Five-month-old juveniles (mean body weight 28.3 ± 2.1 g) were subjected to 0, 3, 6, 9, and 12 ppt
salinities. The hematological parameters were assessed after a period of 20 days rearing at these salinities. After transfer
from fresh water to brackish water, red blood cells, hematocrit, haemoglobin and mean corpuscular haemoglobin decreased, but
mean corpuscular volume increased. Mean corpuscular haemoglobin concentration, white blood cells, monocyte counts, and eosinophil
counts showed no significant variations with increase in environmental salinity. An increase was found in lymphocyte counts
according to the increase of salinity from 0 to 12 ppt, while the fresh water control group maintained basal levels. Decrease
in neutrophil counts was observed in great sturgeon with increase in environmental salinity. These data show significant effect
of salinity on the blood parameters of great sturgeon. 相似文献
50.
We used a goal programming technique to determine the optimal harvest volume for the Iranian Caspian forest. We collected data including volume, growth, wood price at forest roadside, and variable harvesting costs. The allometric method was used to quantify seques- trated carbon. Regression analysis was used to derive growth models. Expected mean price was estimated using wood price and variable harvesting costs. Questionnaire was used to determine the constraints and the equation coefficients of the goal programming model. The optimal volume was determined using the goal programming method according to multipurpose forest management. LINGO software was used for analysis. Results indicated that the optimum volumes of species were 250.25 m3.ha-1 for beech, 59 m3.ha-1 for hornbeam, 73 m3.ha-1 for oak, 41 m3.ha-1 for alder, and 32 m3.ha-1 for other species. The total optimum volume is 455.25 m3.ha-1. 相似文献