Green roof vegetation type affects germination and initial survival of colonizing woody species     

Lori E. Miller  Amy E. Heim  Jeremy Lundholm 《Urban Forestry & Urban Greening》2014,13(4):892-899

Green roofs provide a number of valuable ecosystem services compared to conventional roofs, but may require yearly maintenance. Trees and other woody plants that persist on the roof may damage or overload shallow-substrate green roofs and their removal is a standard maintenance procedure. The germination potential of colonizing species may differ depending on the vegetation surrounding them. The aim of this study was to determine whether the germination of colonizing tree species (Picea glauca and Ulmus glabra) will vary depending on which plant species form the established vegetation seeds land in. To determine germination success, survival, and seed capture ability of the plant canopy, tree seeds were added either directly to the growing medium or atop the plant canopy, in replicated monocultures of 14 species native to Nova Scotia. When seeds were added directly to the soil, no significant difference was detected between the monocultures for germination success or survival for U. glabra or P. glauca. However, when the seeds were added atop the plant canopy, percent germination of U. glabra was significantly higher in Carex argyrantha green roof modules. Overall, sod forming graminoids showed higher germination of U. glabra. The number of seeds reaching the soil was typically lower in vegetation with a denser canopy. This study demonstrates that some vegetation repels colonizing tree species by reducing ground contact. Although these effects differed according to tree species, non-vegetated substrates enhanced seedling persistence. Additionally, the majority of tree seeds that germinated failed to survive a single growing-season on shallow-substrate green roofs.  相似文献   

Assessing common bottlenose dolphin (Tursiops truncatus) population structure in Mississippi Sound and coastal waters of the north central Gulf of Mexico     

Nicole L. Vollmer  Patricia E. Rosel  Keith D. Mullin  Lori H. Schwacke  Lance P. Garrison  Brian C. Balmer  Kevin Barry  Anthony Martinez  Brian M. Quigley  Carrie Sinclair  Todd R. Speakman  Jesse Wicker  Lynsey Wilcox  Eric S. Zolman 《水产资源保护:海洋与淡水生态系统》2021,31(10):2951-2966

1. Population structure of highly mobile marine organisms can be complex and difficult to study, but it is important to understand how populations partition themselves within their environment for accurate assessment of both natural and anthropogenic impacts and successful management. The 2010 Deepwater Horizon oil spill negatively impacted common bottlenose dolphins (Tursiops truncatus) within Mississippi Sound and the surrounding north central Gulf of Mexico (GOMx); however, little was known about their underlying population structure in these waters. Thus, it was unclear how many demographically independent populations were affected by the spill.
2. Common bottlenose dolphin samples were collected throughout inshore waters of Mississippi Sound and coastal waters of the north-central GOMx. Mitochondrial DNA control region sequence data and 19 nuclear microsatellite loci were analysed to determine how many populations are present and characterize their range throughout these waters.
3. Bayesian clustering and migration analyses identified two genetically distinct and demographically independent populations: one predominantly inhabiting Mississippi Sound and adjacent coastal waters, and a second population extending generally from offshore of Mobile Bay, Alabama, east along the Florida Panhandle. Neither of these populations align with the currently delineated management stocks previously used to estimate impacts from the oil spill on common bottlenose dolphins in this portion of the GOMx.
4. These results suggest that revisions may be necessary so that management stocks accurately represent the demographically independent populations present in these waters. Furthermore, better comprehension of underlying population structure will enhance impact assessments on common bottlenose dolphins and provide more appropriate baseline data to support future restoration and conservation objectives.

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Two Reports of Interest     

Lori Hidinger 《Herpetological Monographs》2013,35(2):22-23

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Severe hematometra in a dog with cystic endometrial hyperplasia/pyometra complex.     

Mark T Troxel  Angelyn M Cornetta  Karen F Pastor  Lori E Hartzband  Michael F Besancon 《Journal of the American Animal Hospital Association》2002,38(1):85-89

An 18-month-old, intact female German shepherd dog was presented for evaluation of severe, frank hemorrhage from the vulva. A complete blood count, serum biochemical analysis, and urinalysis supported acute hemorrhage. Abdominal radiographs were unremarkable. Abdominal ultrasound demonstrated that the uterine body and cranial vaginal lumen were fluid-filled and contained hyperechoic, polypoid masses that were suspected to be blood clots. Exploratory laparotomy revealed a mildly enlarged uterus with a diffuse, cystic endometrium; significant amounts of blood and blood clots within the lumen; and several focal accumulations of yellow fluid presumed to be a purulent exudate. Histopathology confirmed severe, cystic, endometrial hyperplasia and pyometra complex.  相似文献   

Molecular biology of male gametogenesis     

Sheila Mccormick  Catherine Curie  Yoram Eyal  Jorge Muschietti  Lori Dircks  Rima Kulikauskas 《Euphytica》1994,79(3):245-250

Pollen development has been studied at a molecular level in several systems that are amenable to genetic or transgenic analysis. We have characterized several tomato genes that are expressed late in pollen development. Our goals in this research were 1) to determine the cis- and tran-acting factors that mediate pollen expression, and 2) to determine the functions of the proteins encoded by these genes. We currently favor the hypothesis that pollen-specific gene expression is mediated in a combinatorial manner. Antisense experiments have indicated an important role for the LAT52 protein during pollen hydration.  相似文献   

Structural basis of a phototropin light switch     

Harper SM  Neil LC  Gardner KH 《Science (New York, N.Y.)》2003,301(5639):1541-1544

Phototropins are light-activated kinases important for plant responses to blue light. Light initiates signaling in these proteins by generating a covalent protein-flavin mononucleotide (FMN) adduct within sensory Per-ARNT-Sim (PAS) domains. We characterized the light-dependent changes of a phototropin PAS domain by solution nuclear magnetic resonance spectroscopy and found that an alpha helix located outside the canonical domain plays a key role in this activation process. Although this helix associates with the PAS core in the dark, photoinduced changes in the domain structure disrupt this interaction. We propose that this mechanism couples light-dependent bond formation to kinase activation and identifies a signaling pathway conserved among PAS domains.  相似文献   

Measurement of corneal sensitivity in 20 ophthalmologically normal alpacas     

Welihozkiy A  Bedenice D  Price LL  Pizzirani S  Pirie CG 《Veterinary ophthalmology》2011,14(5):333-336

Objective To determine corneal sensitivity in 20 healthy adult alpacas (40 eyes) in order to establish reference values. Design Prospective study. Animals Twenty healthy, adult alpacas. Procedures Corneal sensitivity was determined by the corneal touch threshold (CTT) using a Cochet‐Bonnet esthesiometer. Five different regions of the cornea were evaluated (nasal, ventral, lateral, dorsal, and central). Results Corneal touch threshold values (in mm filament length) obtained from five corneal regions demonstrated varying corneal sensitivities. The central region (34.5 ± 7.1 mm) was the most sensitive, followed by the ventral (29.5 ± 7.2 mm), medial (29.3 ± 7.3 mm), dorsal (25.6 ± 6.3 mm), and lateral (21.8 ± 5.7 mm) regions. All pairwise comparisons of regional differences were statistically significant (P < 0.05), except for the comparison between the ventral and medial regions (P = 0.88). Evaluation of the CCT while controlling for age (2–5 years vs. 6–10 years) demonstrated an increased CTT with an increasing age for the central, ventral, medial and lateral regions. A decrease in the CTT of the dorsal region was noted with increasing age. Conclusion and Clinical Relevance Corneal touch threshold values in 40 eyes of 20 healthy adult alpacas were determined using a Cochet‐Bonnet esthesiometer. This study demonstrated the central corneal region to be most sensitive. Values obtained may serve as reference values in subsequent studies.  相似文献   

Use of pimobendan in 170 cats (2006–2010)     

John M. MacGregor  John E. Rush  Nancy J. Laste  Rebecca L. Malakoff  Suzanne M. Cunningham  Natalie Aronow  Daniel J. Hall  Justin Williams  Lori L. Price 《Journal of Veterinary Cardiology》2011,13(4):251-260

Hypothesis/ObjectivesTo describe the therapeutic use of pimobendan in cats, describe the patient population to which it was administered, document potential side effects and report the clinical course following administration of pimobendan in conjunction with standard heart failure therapy. It is hypothesized that cats with advanced heart disease including congestive heart failure from a variety of causes will tolerate pimobendan with a minimum of side effects when used in treatment in conjunction with a variety of other medications.Animals, materials and methodsOne hundred and seventy client owned cats with naturally occurring heart disease, one hundred and sixty four of which had congestive heart failure. Medical records were reviewed and owners and referring veterinarians were contacted for follow-up data. Data collected included pimobendan dose, other medications administered concurrently, data collected at physical examination, presence or absence of heart failure, adverse effects, classification of heart disease, echocardiographic data and survival time. The data were analyzed for significance between the initial visit and any follow-up visits.ResultsAll cats were treated with pimobendan. The median pimobendan dose was 0.24 mg/kg q 12 h. Pimobendan was used in combination with multiple concurrent medications including angiotensin converting enzyme inhibitors, diuretics and anti-thrombotics. Five cats (3.0%) had potential side effects associated with pimobendan. One cat (0.6%) had presumed side effects severe enough to discontinue pimobendan use. Median survival time for 164 cats with congestive heart failure after initiation of pimobendan was 151 days (range 1–870).ConclusionPimobendan appears to be well tolerated in cats with advanced heart disease when used with a variety of concurrent medications. Randomized controlled studies need to be performed to accurately assess whether it is efficacious for treatment of congestive heart failure in cats.  相似文献   

The Effect of Storage on Ammonia Concentration in Canine Packed Red Blood Cells.     

Lori S. Waddell DVM  DACVECC    David E. Holt BVSC  DACVS    Dez Hughes BVSc  MRCVS  DACVECC  Urs Giger PD  DR. med. vet.  DACVIM 《Journal of Veterinary Emergency and Critical Care》2001,11(1):23-26

Objective: To determine the effect of storage on ammonia concentration in canine packed red blood cell (pRBC) units.
Design: In vitro and in vivo study.
Setting: University Veterinary Teaching Hospital.
Interventions: Ammonia concentration was measured in 7 units of canine pRBC prepared in citrate-phosphate-dextrose (CPD) and Adsol^a on Days 1 and 35 of storage. Ammonia was measured in 4 additional units of canine pRBC on Days 0, 7, 14, 21, 28, and 35. Plasma ammonia was also determined in 5 anemic dogs receiving pRBC.
Measurements and Main Results: Ammonia concentration increased from 73 ± 15 mmol/L (mean ± SD) on Day 1 to 800 ± 275 mmpl/L on Day (p<0.001). When measured every 7 days in 4 units of canine pRBC, ammonia concentration increased from 23 ± 8 mmol/L on Day 0 to 179 ± 13 mmol/L (Day 7), 276 ± 56 mmol/L (Day 14). 383 ± 47 mmol/L (Day21), 466 ± 30 mmol/L (Day 28), and 562 ± 27 mmol/L (Day 35) (p<0.05 for all comparisons). In a preliminary study, plasma ammonia concentration measured in blood samples from 5 anemic dogs without primary liver disease immediately before and after transfusion with 5–10 ml/kg of stored pRBC remained in the normal reference range.
Conclusions: The ammonia concentration in stored canine pRBC increased markedly with time. In this preliminary study, ammonia concentrations in dogs without primary liver disease did not increase above the reference range after transfusion with pRBC.  相似文献   

Web-based technology: its effects on small group "problem-based learning" interactions in a professional veterinary medical program     

Schoenfeld-Tacher R  Bright JM  McConnell SL  Marley WS  Kogan LR 《Journal of veterinary medical education》2005,32(1):86-92

The objective of this investigation was to ascertain whether and how the introduction of a new technology (WebCT) influenced faculty teaching styles while facilitating small group problem-based learning (PBL) sessions in a professional veterinary medical (PVM) program. The following questions guided the study: (1) How does the use of technology affect faculty teaching behaviors? (2) Do the facilitators' interactions with WebCT technology change over the course of one semester? (3) What is the perceived impact of WebCT on facilitators' role in PBL? The study employed a combination of qualitative (case study) and semi-quantitative (survey) methods to explore these issues. Nine clinical sciences faculty members, leading a total of six PBL groups, were observed over the course of an academic semester for a total of 20 instructional sessions. The qualitative data gathered by observing faculty as they facilitated PBL sessions yielded three major themes: (1) How do PBL facilitators adapt to the addition of WebCT technology? (2) Does this technology affect teaching? and (3) How do PBL facilitators interact with their students and each other over the course of a semester? No direct evidence was found to suggest that use of WebCT affected teaching behaviors (e.g., student-centered vs. teacher-centered instruction). However, all facilitators showed a moderate increase in comfort with the technology during the semester, and one participant showed remarkable gains in technology skills. The teaching theme provided insight into how facilitators foster learning in a PBL setting as compared to a traditional lecture. A high degree of variability in teaching styles was observed, but individuals' styles tended to remain stable over the course of the semester. Nevertheless, all facilitators interacted similarly with students, in a more caring and approachable manner, when compared to the classroom or clinic atmospheres.  相似文献