Ganglioneuroma in the small intestine of a juvenile pig     

Murakami M  Sakai H  Mizutani K  Yanai T 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2011,73(11):1501-1504

A mass was located in the small intestine of a slaughtered 6-month-old male Landrace-cross pig that had no clinical abnormalities. This egg-shaped well-circumscribed mass was situated in the submucosal and muscular tissue layers and protruded into the lumen. Histopathologically, the tumor comprised discrete or aggregated ganglion and schwannian cells in neuropil-like tissue. Some ganglion cells contained Nissl substance in their cytoplasm. The ganglion cells stained positive for neuron-specific enolase, class III β-tubulin, neurofilament, and synaptophysin; the schwannian cells stained positive for vimentin, S-100 protein, and glial fibrillary acidic protein. The tumor was diagnosed as a ganglioneuroma in accordance with these findings. Here, we have reported detailed immunohistochemical findings in addition to the histopathological features of a swine ganglioneuroma.  相似文献   

Immunobiotic lactic acid bacteria beneficially regulate immune response triggered by poly(I:C) in porcine intestinal epithelial cells     

Hosoya S  Villena J  Shimazu T  Tohno M  Fujie H  Chiba E  Shimosato T  Aso H  Suda Y  Kawai Y  Saito T  Alvarez S  Ikegami S  Itoh H  Kitazawa H 《Veterinary research》2011,42(1):111

ABSTRACT: This study analyzed the functional expression of TLR3 in various gastrointestinal tissues from adult swine and shows that TLR3 is expressed preferentially in intestinal epithelial cells (IEC), CD172a+CD11R1high and CD4+ cells from ileal Peyer's patches. We characterized the inflammatory immune response triggered by TLR3 activation in a clonal porcine intestinal epitheliocyte cell line (PIE cells) and in PIE-immune cell co-cultures, and demonstrated that these systems are valuable tools to study in vitro the immune response triggered by TLR3 on IEC and the interaction between IEC and immune cells. In addition, we selected an immunobiotic lactic acid bacteria strain, Lactobacillus casei MEP221106, able to beneficially regulate the anti-viral immune response triggered by poly(I:C) stimulation in PIE cells. Moreover, we deepened our understanding of the possible mechanisms of immunobiotic action by demonstrating that L. casei MEP221106 modulates the interaction between IEC and immune cells during the generation of a TLR3-mediated immune response.  相似文献   

A refined inoculation method to evaluate false smut resistance in rice   总被引：1，自引：0，他引：1  

Taketo Ashizawa  Mami Takahashi  Jouji Moriwaki  Kazuyuki Hirayae 《Journal of General Plant Pathology》2011,77(1):10-16

False smut, caused by Ustilaginoidea virens, is a serious disease of rice worldwide. To evaluate false smut resistance in rice, we developed a method combining the cultivation of the main culm of rice plants in the greenhouse and rapid preparation of a conidial suspension to inject into the leaf sheath. The method was used to evaluate false smut resistance in 18 varieties/lines of rice. For comparison, field trials were also carried out in 2007 and 2008. The results indicated that the greenhouse method was more reproducible than field trials: commercial varieties tested were resistant; almost all the forage varieties were highly susceptible; and blast-resistant varieties/lines were mostly resistant to false smut. Thus, this inoculation method will be useful for determining the level of false smut resistance in rice and for breeding resistant varieties.  相似文献   

Plasma melatonin secretion rhythms in cattle under varying seasonal conditions     

Mizuna Ogino  Akihiro Matsuura  Atusi Yamazaki  Mami Irimajiri  Shiro Kushibiki  Hiroyuki Shingu  Etsuko Kasuya  Yoshihisa Hasegawa  Koichi Hodate 《Animal Science Journal》2013,84(3):253-257

The purpose of the present study was to investigate the secretion cycles of melatonin (MEL) in cattle over the course of four seasons. Four female Japanese Shorthorn cattle under free‐stall and ad libitum feeding conditions were used, and plasma MEL concentrations were measured over a 48 h period at 1 h intervals. The time‐series data were analyzed by spectral analysis, and the cycle hour was determined. Data indicated that the secretion cycle for MEL was approximately 23.5 h for all four seasons. The area under the curve of MEL from start to end of experiment for 48 h did not differ significantly among the four seasons. However, the duration of high MEL secretion which defined the duration time of the values were more than 10 pg/mL and differed significantly among the four seasons. In conclusion, this study, which was the first to use spectral analysis to evaluate the cyclic rhythm of MEL in cattle, revealed that MEL secretion cycles did not differ among the seasons. These findings are inconsistent with previous study results in that previous reports suggested that the MEL secretion cycle differed under different lighting conditions.  相似文献   

Biological rhythms related to metabolism in Japanese Shorthorn cattle under varying environments and management techniques     

Mizuna Ogino  Akihiro Matsuura  Atusi Yamazaki  Mami Irimajiri  Hideyuki Takahashi  Tokushi Komatsu  Shiro Kushibiki  Hiroyuki Shingu  Etsuko Kasuya  Yoshihisa Hasegawa  Koichi Hodate 《Animal Science Journal》2013,84(6):513-526

Plasma insulin (INS), thyroxin (T₄), glucose (GLU), non‐esterified fatty acid (NEFA), blood urea nitrogen (BUN), rectal temperature (RT) and eating behavior were evaluated in Japanese Shorthorn cattle under varying external environments and management techniques. Serial blood collection and assessments of RT and eating behavior were performed over 48 h in the spring, summer, autumn and winter in four female cattle reared under either free‐stall and ad libitum feeding (FA) conditions or tie‐stall and restricted feeding (TR) conditions. Cycle patterns for each parameter were examined using spectral analysis, and correlations between parameters were investigated using cross‐spectral analysis. Rhythms for all parameters, except eating behavior and T₄, did not differ significantly among the varied external environments and between management techniques, although seasonal differences in the concentration or value of parameters were observed. An approximate 3‐ or 4‐h rhythm cycle detected in T₄, GLU, NEFA, BUN, and RT might be the common metabolic rhythm. Under both conditions, the metabolite levels showed strong correlations with eating behavior. Moreover, GLU positively correlated with INS at lag time of 0 h, as did eating behavior and RT.  相似文献   

Expression of inflammatory-related factors in porcine anterior pituitary-derived cell line   总被引：2，自引：0，他引：2  

Nagai Y  Ogasawara H  Taketa Y  Aso H  Kanaya T  Miyake M  Watanabe K  Ohwada S  Muneta Y  Yamaguchi T 《Veterinary immunology and immunopathology》2008,124(3-4):201-208

Recent studies have shown that undifferentiated stem cells act as immunomodulators. To investigate the immunomodulatory function of the progenitor cells of the anterior pituitary gland, we attempted to establish a stem/progenitor cell line from the porcine anterior pituitary gland, and to detail its inflammatory cytokine expression. A cloned cell line from the porcine anterior pituitary gland was established and was designated as the porcine anterior pituitary-derived cell line (PAPC). PAPC expressed the mRNA of Nanog and Oct-4, and showed positive immunoreactivity for beta-catenin and Hes1 in its nucleus. PAPC grew stably by repeated passage and rapidly in the EGF and bFGF containing medium. RT-PCR showed that PAPC expressed mRNA of IL-1alpha, IL-6, IL-12, IL-15, IL-18 and TLR4. PAPC expressed S100alpha and IL-18 protein, which was localized in the marginal epithelial cells of Rathke's pouch. These results suggest that PAPC is a stem/progenitor cell and may regulate anterior pituitary cell function through an immuno-endocrine pathway.  相似文献   

Presence of neuropeptide Y in somatotrophs of cattle     

Ogasawara H  Aso H  Nagai Y  Matsumoto K  Okamura H  Tanaka S  Watanabe K  Ohwada S  Yamaguchi T 《Domestic animal endocrinology》2008,35(3):274-280

Neuropeptide Y (NPY), a 36-amino acid member of the pancreatic polypeptide family, was found to be present by immunohistochemistry in the bovine adenohypophysis. NPY mRNA expression was confirmed in the adenohypophysis by RT-PCR. NPY immunoreactivity was present in about 38% of adenohypophyseal cells in the pars distalis. However, NPY immunoreactive cells (NPY-ir cells) were scarce in the zona tuberalis. Immunohistochemistry of NPY and specific hormones using mirror sections revealed that NPY was colocalized in GH immunoreactive cells. Over 90% of somatotrophs corresponded to NPY-ir cells. These results indicate that endogenous NPY is present in the bovine somatotroph and may act as an endocrine intercellular mediator in the adenohypophysis.  相似文献   

Role of SraP in adherence of Staphylococcus aureus to the bovine mammary epithelia     

Takamatsu D  Hata E  Osaki M  Aso H  Kobayashi S  Sekizaki T 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2008,70(7):735-738

SraP, a platelet-binding surface protein of Staphylococcus aureus, is involved in the pathogenesis of infective endocarditis. In this study, we investigated the importance of SraP in the pathogenesis of bovine mastitis. By means of PCR, sraP was detected in all the isolates tested from bovine bulk milk and humans. However, SraP was not expressed on the cell surface in half of the bovine isolates. Moreover, disruption of sraP did not affect the ability of S. aureus to adhere to cultured bovine mammary epithelial cells. These results suggest that SraP does not seem to be an important factor for S. aureus to adhere to the bovine mammary epithelia.  相似文献   

Fertility after artificial insemination using a soybean-based semen extender in sheep     

Fukui Y  Kohno H  Togari T  Hiwasa M  Okabe K 《The Journal of reproduction and development》2008,54(4):286-289

The present study aimed to compare the fertility of ewes intrauterinally inseminated with frozen-thawed semen using a soybean-based semen extender (AndroMed) with those of ewes intrauterinally inseminated with frozen-thawed semen using a Tris-based extender containing either egg yolk or BSA. Suffolk ewes (n=104) were treated with an intravaginal sponge containing 40 mg fluoroprogesterone acetate (FGA) for 12 days and an intramuscular injection of 500 IU equine chorionic gonadotropin to induce estrus and ovulation during the non-breeding season (July, 2007). Intrauterine insemination was carried out 40-46 h after removal of the FGA sponge (n=90), regardless of the incidence of estrus. The pregnancy rates were not significantly different among the semen extenders containing egg yolk (64.5%) or BSA (58.6%) and AndroMed extender (56.7%). The lambing rates (64.5, 55.2 and 56.7% for the semen extenders containing egg yolk, BSA and AndroMed, respectively) and prolificacy (1.59 to 1.75) were also not significantly different. The present results indicate that an egg yolk-containing semen extender can be replaced with the non-animal derived extender AndroMed, which could be used for intrauterine insemination using frozen-thawed ram semen without reducing fertility.  相似文献   

Meiotic resumption of porcine immature oocytes is prevented by ooplasmic Gsalpha functions     

Morikawa M  Seki M  Kume S  Endo T  Nishimura Y  Kano K  Naito K 《The Journal of reproduction and development》2007,53(6):1151-1157

A high cyclic adenosine monophosphate (cAMP) level in fully-grown immature oocytes prevents meiotic resumption. In Xenopus, inhibitory cAMP is synthesized within oocytes depending on a stimulatory alpha-subunit of G-protein (Gsalpha). In the present study, we examined whether ooplasmic Gsalpha is involved in meiotic arrest of porcine oocytes. First, we studied the presence of Gsalpha molecules in porcine oocytes by immunoblotting, and this suggested the presence of reported isoforms (45 and 48 kDa) not only in cumulus cells but also in porcine oocytes. Then we injected an anti-Gsalpha antibody into porcine immature oocytes and found that inhibition of ooplasmic Gsalpha functions significantly promoted germinal vesicle breakdown of the oocytes, whose spontaneous meiotic resumption was prevented by 3-isobutyl-l-methylxanthine (IBMX) treatment. Although cyclin B synthesis and M-phase promoting factor (MPF) activation were largely prevented until 30 h of culture in IBMX-treated oocytes, injection of anti-Gsalpha antibody into these oocytes partially recovered cyclin B synthesis and activated MPF activity at 30 h. These results suggest that meiotic resumption of porcine oocytes is prevented by ooplasmic Gsalpha, which may stimulate cAMP synthesis within porcine oocytes, and that synthesized cAMP prevents meiotic resumption of oocytes through the signaling pathways involved in MPF activation.  相似文献