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21.
Leila Beigom Hejazian Banafshe Esmaeilzade Fatima Moghanni Ghoroghi Fatemeh Moradi Marzieh Beigom Hejazian Anahita Aslani Mehrdad Bakhtiari Masoud Soleimani Maliheh Nobakht 《Iranian Biomedical Journal》2012,16(4):193-201
Background
The aim of this study was to fabricate the poly caprolactone (PCL) aligned nanofiber scaffold and to evaluate the survival, adhesion, proliferation, and differentiation of rat hair follicle stem cells (HFSC) in the graft material using electrospun PCL nanofiber scaffold for tissue engineering applications.Methods
The bulge region of rat whisker was isolated and cultured in DMEM: nutrient mixture F-12 supplemented with epidermal growth factor. The morphological and biological features of cultured bulge cells were observed by light microscopy using immunocytochemistry methods. Electrospinning was used for production of PCL nanofiber scaffolds. Scanning electron microscopy (SEM), 3-(4, 5-di-methylthiazol- 2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay, and histology analysis were used to investigate the cell morphology, viability, attachment and infiltration of the HFSC on the PCL nanofiber scaffolds.Results
The results of the MTT assay showed cell viability and cell proliferation of the HFSC on PCL nanofiber scaffolds. SEM microscopy images indicated that HFSC are attached, proliferated and spread on PCL nanofiber scaffolds. Also, immunocytochemical analysis showed cell infiltration and cell differentiation on the scaffolds.Conclusion
The results of this study reveal that PCL nanofiber scaffolds are suitable for cell culture, proliferation, differentiation and attachment. Furthermore, HFSC are attached and proliferated on PCL nanofiber scaffolds.Key Words: Nanofiber, Electrospinning, Stem cells, Tissue engineering 相似文献22.
Marzieh Safari Farzaneh Nahari Alishah Hossein Kari Dolatabad Udonna Ndu Cristian P. Schulthess Ali Sorooshzadeh 《植物养料与土壤学杂志》2019,182(3):463-476
Cadmium (Cd) is a major pollutant in soils as a result of extensive use of fertilizers, mining and industrial discharges. Zinc (Zn) and certain bacterial species have been known to alleviate Cd toxicity in plants. In this study, the individual and combined effects of the application of Zn and Pseudomonas species with the aim of reducing Cd stress in wheat cultivars were investigated. Plants (durum wheat and bread wheat) were exposed to different concentrations of Cd and Zn, and either P. putida or P. fluorescens in a growth chamber. Concentrations of Zn, Cd, chlorophyll (Chl), carotenoid, hydrogen peroxide (H2O2), and malondialdehyde (MDA), as well as antioxidant enzyme activities were assayed. The addition of Zn in soils reduced the toxicity of Cd in durum wheat more than in bread wheat even though there was more uptake of Zn in bread wheat. Analysis of variance showed that by using Zn fertilizer and Pseudomonas species the amounts of peroxidase (POD), polyphenoloxidase (PPO), MDA, and H2O2 were reduced at three growth stages. Surprisingly, with increasing Zn concentration, Cd concentration in plant tissue was slightly increased, which suggests that adding Zn to soil could facilitate Cd desorption from soil particles. Application of Pseudomonas and Zn could be a promising solution to reduce detrimental effects of Cd, especially in durum wheat. 相似文献
23.
Reza Rezanejad Marzieh Heidarieh Mojtaba Raeisi Alireza Alishahi Gholamreza Rafiee 《Journal Of Aquatic Food Product Technology》2019,28(5):478-494
The objective of this study was to evaluate the effect of different forms and doses of rosemary on chemical, microbial, and sensory properties of rainbow trout fed nine different diets: control (C), 20 g.kg?1 rosemary powder (20RP), 40 g.kg?1 rosemary powder (40RP), 0.3 g.kg?1 rosemary extract (0.3RE), 0.6 g.kg?1 rosemary extract (0.6RE), 0.15 g.kg?1 rosemary nanopowder (0.15RNP), 0.3 g.kg?1 rosemary nanopowder (0.3RNP), 0.15 g.kg?1 butylated hydroxyanisole (BHA) (0.15BHA), and 0.3 g.kg?1 BHA (0.3BHA). After 8 weeks’ feeding, the fish fillets were sampled on the 1st, 4th, 8th, 12th, and 16th days and then stored on 4°C. Lower value of pH, peroxide value (PV), total volatile base nitrogen (TVB-N), free fatty acids (FFA), and thiobarbituric acid (TBA) were reported in fish fed with RP, RE, RNP, and BHA; among them, RNP groups had the lowest properties (p < 0.05). Furthermore, lower total viable aerobic bacterial counts (TVC) and psychotropic counts (PTC) were observed in the fillets of the fish fed with rosemary treatments, especially in RNP treatments (7.52–9.41 log10 CFU.g?1), along with a slower spoilage in terms of sensory factors (texture, color, odor, and overall) on the 16th day. Finally, use of natural antioxidant nanorosemary in the diet may positively affect fish fillet quality and delay post-mortem deterioration. 相似文献
24.
Marzieh Rahimi Niyyat Mohammad Azizzadeh Javad Khoshnegah 《Topics in companion animal medicine》2018,33(4):150-155
Recent discoveries have shown that the chances of a dog developing a behavioral disorder may depend upon a number of factors including nutrition. The current pilot study was designed to provide an assessment of the efficacy of a dietary supplement containing omega-3 fatty acids, magnesium, and zinc on some common behavioral disorders in a population of Iranian domestic dogs. In total, 48 dogs including 6 dogs without any behavior disorder (control group) and 42 dogs with at least 1 common behavioral disorder, namely excessive activity, inappropriate elimination, fearfulness, destructiveness, and aggression toward unfamiliar people and dogs (test group), were given daily oral dose of gelatin capsules of fish oil supplements containing 330 mg eicosapentaenoic acid and 480 mg docosahexaenoic acid. Moreover, all dogs received 12-15 mg/kg of magnesium citrate and 5 mg/kg of zinc sulfate. Data were obtained using a questionnaire that dog owners were invited to fill out 2 times before (Days 0 and 42) and 2 times after the supplement treatment period (Days 84 and 126). The questionnaire asked owners whether their dog had exhibited any of the 6 common behavioral disorders on a 5-point Likert-like scale ranging from 0 (never or very rarely) to 4 (very often). The results showed no significant changes for any of the evaluated behavior disorders scale in the control group. In dogs with behavior disorders, results showed a significant reduction in the median score for the severity of fearfulness (P?=?.0083), destructiveness (P?=?.002), and inappropriate elimination (P < .001). In addition, there were no significant differences in the median score for the severity of excessive activity (P = .162), aggression toward dogs (P = .281), and aggression toward unfamiliar people (P = .09) during the course of the study. Results of the study reported here support the hypothesis that a combination of omega-3 fatty acids, magnesium, and zinc may improve some of the behavioral disorders. 相似文献
25.
Marzieh S. Javadi Javad Mokhtari Mahdi Nouri Mohammad J. Mehdipour 《Fibers and Polymers》2013,14(2):195-200
Two novel cationic softener containing mono-s-chloro triazinyl reactive dyes together with their analogues were designed. The dyes were synthesized via reacting an N,N-dimethyldodecylamine with p-nitrobenzyl bromide. The resultant was reduced using stannous chloride and hydrochloric acid to produce the primary amine. The quaternary ammonium salt containing primary amine was then diazotized to produce diazonium salt part of azo dye. The diazonium salt was then coupled to H-acid/J-acid reacted with cyanuric chloride and sulfanilic acid. The analogue dyes were prepared via the same route without quaternary ammonium salt making stage. The chemical structures of the novel dyes were characterized by FTIR, 1H-NMR, and elemental analysis. The spectroscopic properties of the dyes were determined in terms of λ max and ? max in aqueous solution. 相似文献
26.
SayedHamid Matinkhah Marzieh Taabe Mohammadreza Raoofi S. Mojtaba Matinkhah 《Arid Land Research and Management》2016,30(3):304-319
The success of plantation, establishment, and growth of trees and shrubs is an important concern in afforestation efforts. There have been extensive efforts to use Haloxylon ammodendron in many desert areas throughout the world in desert combating projects, but these efforts have not always been successful, despite high costs. In this study, we propose a new application of fuzzy decision-making method for selecting suitable sites for the growth of this species. We determine, based on available autecological studies, the most effective factors in desert habitats, and a fuzzy membership function is designed for each of the factors. A suitability index is constructed by unifying the fuzzified factors. This model is then applied to an actual plantation area to evaluate the suitability of different parts of this trial area. Finally, the predicted results are compared to the actual rate of success, based on the canopy cover percentage. The coefficient of determination for the observed canopy cover percentage plotted against the predicted suitability values is higher than 98%, which demonstrates that the presented fuzzy decision-making approach is potentially a powerful tool in site selection for this and, probably, other species. 相似文献
27.
Sayyah M Kaviani B Khoshkholgh-Sima B Bagheri M Olad M Choopani S Mahdian R 《Iranian Biomedical Journal》2012,16(1):25-32
Background: Hippocampal damages, which are accompanied by inflammation, are among the main causes of epilepsy acquisition. We previously reported that chronic intracerebroventricular (i.c.v.) injection of lipopolysaccharide (LPS) modulates epileptogenesis in rats. There is a network of gap junction channels in the hippocampus that contribute to epileptogenesis. Gap junction channels are formed by oligomeric protein subunits called connexins (Cx). Astrocytic Cx43 and neuronal Cx36 are expressed in the hippocampus. In order to find out the possible role of gap junctions in seizure-modulating effect of LPS and neuroinflammation, we studied the effect of central administration of LPS on expression of Cx36 and Cx43 in rat hippocampus. Methods: LPS, 2.5 µg/rat/day, was injected i.c.v. to male Wistar rats for 14 days. mRNA and protein abundance of Cx36, Cx43 and IL1-β were measured in rat hippocampus by real time-PCR, Western blot and ELISA techniques, at the beginning, in the middle, and at the end of the treatment period. Results: IL1-β protein level was significantly increased 6 h after first injection of LPS. Cx36 and Cx43 mRNA expression did not alter during chronic administration of LPS. A selective decrease in Cx43 protein expression was observed after 7 injections of LPS. Conclusion: It is suggested that Cx43 containing gap junctions in the hippocampus is down-regulated in response to chronic injection of LPS. This event can inhibit propagation of toxic and noxious molecules to neighboring cells and modulate hippocampal excitability and epileptogenesis. Key Words: Connexin36 (Cx36), Connexin43 (Cx43), Interleukin-1β (IL1-β ) 相似文献
28.
Foliar application of nitrogenous organic compounds effects on the quantity and quality of Echinacea purpurea were investigated. This experiment was conducted in a randomized complete blocks design with three replications in growing season of years 2011–2012 and 2012–2013. Treatments included Humiforte (H) and Fosnutren (F) organic fertilizers with concentrations of (0.5, 0.75, 1, 1.5, and 2?L ha?1). Most of the vegetative traits, all of the caffeic acid derivatives measured in aerial parts and roots were significantly affected by foliar application of these nitrogen-contained organic compounds. Generally, best quantitative and qualitative performance belonged to F(2) and F(0.5) treatments, respectively. The application of organic compounds led to significant increase in qualitative and quantitative traits in Echinacea which could be considered as a strategy for increasing medicinal metabolites production in this plant. 相似文献