Arrangement of cortical microtubules in compression wood tracheids of Taxus cuspidata visualized by confocal laser microscopy     

Osamu Furusawa  Ryo Funada  Yutaka Murakami  Jun Ohtani 《Journal of Wood Science》1998,44(3):230-233

Cortical microtubules (MTs) in differentiating compression wood tracheids of Taxus cuspidata stems were visualized by confocal laser microscopy. They were oriented obliquely at an angle of about 45° to the tracheid axis during formation of the secondary wall. Artificial inclination altered the pattern of alignment of MTs. Banding MTs were helically oriented late during the formation of the secondary walls. These results indicate that MTs might control the orientation and localized deposition of cellulose microfibrils in the secondary walls of compression wood tracheids.Part of this report was presented at the 46th annual meeting of the Japan Wood Research Society, Kumamoto, April 1996  相似文献   

Genetic variation in radish (Raphanus sativus L.) germplasm from Pakistan using morphological traits and RAPDs     

Malik Ashiq Rabbani  Yoshie Murakami  Yasuhisa Kuginuki  Kenji Takayanagi 《Genetic Resources and Crop Evolution》1998,45(4):307-316

Genetic diversity of 30 radish (Raphanus sativus L.) accessions was investigated at the phenotypic level with morphological characters and at the DNA level using the random amplified polymorphic DNA (RAPD) technique. Thirty-six morpho-physiological traits were recorded from seedling stage to harvest. The 31 primers used generated 202 RAPD bands, of which 158 (78.2%) were polymorphic. Multivariate procedures were used to classify the germplasm on the basis of phenotypic traits and RAPD fragments. Dendrograms were generated for the Euclidean distance from the morphological data and the Nei's genetic distance from the RAPD markers. Phenotypically, all the accessions were classified into four major groups corresponding to the different forms of cultivated radish. The morphological diversity existing within each of these groups suggested that they should be discriminated into the three botanical convarieties, sativusT (large-rooted), caudatus (pod-type) and oleifer (oilseed-type). Clustering of the accessions did not show any pattern of association between the morphological characters and the collection sites. Instead, landrace groups were associated with their morphological similarities and horticultural uses. On the other hand, the intra-specific genetic relationships of several accessions based on RAPD analysis were related primarily to their collection sites rather than to their phenotypic affinities. The level of polymorphism exhibited by the various convarieties could be exploited in genetic mapping populations to tag economically important traits. These genotypes also could serve as a useful germplasm source for root, leaf, pod and seed. This preliminary study of traditional radish landraces from Pakistan provides useful information regarding their horticultural potential.  相似文献   

Identification of coumarins from lemon fruit (Citrus limon) as inhibitors of in vitro tumor promotion and superoxide and nitric oxide generation.   总被引：1，自引：0，他引：1  

Y Miyake  A Murakami  Y Sugiyama  M Isobe  K Koshimizu  H Ohigashi 《Journal of agricultural and food chemistry》1999,47(8):3151-3157

Three coumarins were isolated as siginificant inhibitors of tumor promoter 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced Epstein-Barr virus (EBV) activation in Raji cells from the peel of lemon fruit. They were identified as 8-geranyloxypsolaren (LE-1), 5-geranyloxypsolaren (bergamottin, LE-2), and 5-geranyloxy-7-methoxycoumarin (LE-3), respectively, by spectroscopic analysis. Three isolates had no potential O(2)-scavenging and markedly suppressed TPA-induced superoxide (O(2)(-)) generation in differentiated human promyelocytic HL-60 cells. Furthermore, LE-1 and LE-3 reduced both lypopolysaccharide (LPS) and interferon-gamma (IFN-gamma)-induced nitric oxide (NO) generation in mouse macrophage RAW 264.7 cells. Similarly, they were found to be NO generation inhibitors rather than scavengers by measuring the extracellular L-citrulline levels. The occurrence of these coumarins in a lemon fruit was abundant in the flavedo of the peel based on quantitative analysis using high-performance liquid chromatography (HPLC). The present study suggests that the coumarins in lemon fruit are promising chemopreventive agents by inhibiting radical generation.  相似文献   

Cloning and mapping of three pig acyl-CoA dehydrogenase genes.     

N Hamasima  H Suzuki  T Ito  Y Murakami  T Okamoto 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》1999,61(4):417-419

To investigate the structure of porcine genes involved in the beta-oxidation of fatty acid, we isolated the short-chain acyl-CoA dehydrogenase (SCAD), medium-chain acyl-CoA dehydrogenase (MCAD), and long-chain acyl-CoA dehydrogenase (LCAD) genes from the pig. The cDNA of SCAD, MCAD and LCAD genes were 1899 bp, 1835 bp 1835 bp and 1704 bp long and coded for 413-aa, 422-aa and 430-aa precursor proteins, respectively. Three genes, SCAD, MCAD and LCAD were mapped to 14p16.2-23.2, 6q32.4-33, and 15q24.2-26.3, respectively.  相似文献   

Growth and maturation of Pacific saury Cololabis saira under laboratory conditions     

Mitsuhiro Nakaya  Taizo Morioka  Kyouhei Fukunaga  Naoto Murakami  Takashi Ichikawa  Sachio Sekiya  Satoshi Suyama 《Fisheries Science》2010,76(1):45-53

This study details the growth and maturation processes of Pacific saury Cololabis saira from eggs to first spawning under laboratory conditions. They were reared at 20°C, and fed almost to satiation every day. There was no significant difference in the knob length (KnL) between males and females, and so data for both sexes were combined in the following Gompertz growth formula: KnL _t  = 277.1 exp[?exp(?0.015 (t ? 83.8))]. The first spawning was observed 243 days after hatching (DAH). At the beginning of spawning, when the mean KnL was about 250 mm, only several hundred eggs were spawned at most. The spawning continued, and the number of spawned eggs increased notably after 260 DAH. Correlation and stepwise multiple regression analysis of the gonad somatic index (GSI) of Pacific saury versus KnL, CF, and DAH revealed that only DAH (R = 0.88 and 0.72 for males and females, respectively) was significantly correlated with GSI of Pacific saury (P < 0.001). This result suggests that DAH is one of the most influential factors for maturation in this species.  相似文献   

Changes in volatile compounds of living Pacific oyster Crassostrea gigas during air-exposed storage     

Kawabe  Shinya  Murakami  Haruna  Usui  Masakatsu  Miyasaki  Taiko 《Fisheries Science》2019,85(4):747-755

Fisheries Science - The changes in volatile compounds of living oysters Crassostrea gigas with shells under air-exposed storage for 7&nbsp;days at 5 and 20&nbsp;°C were investigated...  相似文献   

The importance of riparian subtropical lucidophyllous forest to odonate conservation     

Masaru Sakai  Shin‐ichi Suda  Taichi Okeda  Ryosuke Nomura  Izumi Washitani 《水产资源保护:海洋与淡水生态系统》2019,29(5):682-692

Expressed sequence tag analysis of phyllosomas and hemocytes of Japanese spiny lobster Panulirus japonicus     

Duangjai Pisuttharachai  Motoshige Yasuike  Hideaki Aono  Keisuke Murakami  Hidehiro Kondo  Takashi Aoki  Ikuo Hirono 《Fisheries Science》2009,75(1):195-206

Two complementary DNA (cDNA) libraries were constructed from phyllosomas and hemocytes of adult Japanese spiny lobster Panulirus japonicus and a total of 2,673 expressed sequence tags (ESTs) were obtained. After assembly and clustering, 450 and 458 unique sequences were found from the phyllosoma and hemocyte cDNA libraries, respectively. Of these, 114 and 220 ESTs showed significant homologies with known genes in the National Centre for Biotechnology Information (NCBI) database. The remaining sequences were of unknown function. Immune-related genes found in this study include lectin, proteinase inhibitor, prophenoloxidase, heat-shock protein, antimicrobial peptide, and a few putative defense-related proteins.  相似文献   

Correction to: A new and simple method for measuring in situ field-saturated hydraulic conductivity using a falling-head single cylinder     

Noborio  Kosuke  Ito  Yuki  He  Hailong  Li  Min  Kojima  Yuki  Hara  Hirofumi  Mizoguchi  Masaru 《Paddy and Water Environment》2018,16(1):81-87

Paddy and Water Environment - Hydraulic properties of soil play important roles in water and temperature regimes. Measuring hydraulic properties has been studied for decades in the laboratory and...  相似文献   

Determination of the spawning history of Pacific saury <Emphasis Type="Italic">Cololabis saira</Emphasis> from rearing experiments: identification of post-spawning fish from histological observations of ovarian arterioles     

Satoshi Suyama  Akio Shimizu  Sayoko Isu  Hitomi Ozawa  Taizo Morioka  Mitsuhiro Nakaya  Toru Nakagawa  Naoto Murakami  Takashi Ichikawa  Yasuhiro Ueno 《Fisheries Science》2016,82(3):445-457

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