Involvement of histone H2B monoubiquitination in the regulation of mouse preimplantation development     

Masatoshi OOGA  Masataka G. SUZUKI  Fugaku AOKI 《The Journal of reproduction and development》2015,61(3):179-184

Histone H2B monoubiquitination (H2Bub1) plays an important role in developmental regulation in various vertebrate species. However, the role of H2Bub1 in mammalian preimplantation development remains unclear. In the present study, we examined the role of H2Bub1 in the regulation of mouse preimplantation development. Based on immunocytochemical analysis using an anti-H2Bub1 antibody, no H2Bub1 signal was detected in the metaphase chromosomes of unfertilized oocytes or the pronuclei of early 1-cell stage embryos, but a weak signal was observed in late 1-cell stage embryos. The signal increased after cleavage into the 2-cell stage, and thereafter a strong signal was observed until the blastocyst stage. To assess the significance of H2Bub1 in the regulation of preimplantation development, RNF20 (an H2B-specific ubiquitin E3 ligase) was knocked down using small interfering RNA (siRNAs). In embryos treated with siRNA, the levels of Rnf20 mRNA and H2Bub1 decreased at the 4-cell and morula stages. Although these embryos developed normally until the morula stage, only one-third developed into the blastocyst stage. These results suggested that H2Bub1 is involved in the regulation of preimplantation development.  相似文献   

Effect of monensin withdrawal on rumen fermentation,methanogenesis and microbial populations in cattle          下载免费PDF全文

Arfan Abrar  Takamitsu Tsukahara  Makoto Kondo  Tomomi Ban‐Tokuda  Wang Chao  Hiroki Matsui 《Animal Science Journal》2015,86(9):849-854

This study was designed to obtain information on the residual influence of dietary monensin on ruminant fermentation, methanogenesis and bacterial population. Three ruminally cannulated crossbreed heifers (14 months old, 363 ± 11 kg) were fed Italian ryegrass straw and concentrate supplemented with monensin for 21 days before sampling. Rumen fluid samples were collected for analysis of short chain fatty acid (SCFA) profiles, monensin concentration, methanogens and rumen bacterial density. Post‐feeding rumen fluid was also collected to determine in vitro gas production. Monensin was eliminated from the rumen fluid within 3 days. The composition of SCFA varied after elimination of monensin, while total production of SCFA was 1.78 times higher than on the first day. Methane production increased 7 days after monensin administration ceased, whereas hydrogen production decreased. The methanogens and rumen bacterial copy numbers were unaffected by the withdrawal of monensin.  相似文献   

浅议我国对流浪动物的管理     

班曼曼  郑海红  贾广敏 《中国动物检疫》2014,31(10):38-40

我国宠物数量的快速增长,带来庞大了的流浪动物群。流浪动物群体的不断增长不仅影响着城市面貌,也给社会公共卫生安全埋下了隐患,引起了广泛的社会关注。通过研究我国流浪动物现状及不同国家和地区对流浪动物的立法及管理措施,针对我国流浪动物存在的隐患和管理问题,探讨我国流浪动物的管理措施和对策,以提高我国流浪动物管理水平,维护公共卫生安全。  相似文献   

Effect of dietary astaxanthin rich yeast,Phaffia rhodozyma,on meat quality of broiler chickens     

Ganzaya Perenlei  Hitomi Tojo  Toru Okada  Masatoshi Kubota  Motoni Kadowaki  Shinobu Fujimura 《Animal Science Journal》2014,85(10):895-903

We evaluated effects of dietary supplementation with astaxanthin (Ax)‐rich yeast, Phaffia rhodozyma (Xanthophyllomyces dendrorhous), on broiler chicken meat quality. Fourteen‐day‐old female Ross broilers were divided into three groups: control group, Ax‐free diet; Ax 10 group, 10 mg/kg Ax diet; and Ax 20 group, 20 mg/kg Ax diet for 28 days. At 42 days old, chickens were slaughtered, and then growth performance, meat quality and sensory attributes were analyzed. Compared with the control, a* values increased significantly after slaughter and 48 h postmortem for Ax 20 samples (P < 0.05) and for b* values in Ax 20 and Ax 10 groups (P < 0.05). Cooking loss decreased in the Ax 20 group (P < 0.05). After 120 h aging, contents of several free amino acids and total free amino acid content of Ax 20 group were significantly higher than the control (P < 0.05). In sensory evaluation, meat texture attributes improved significantly in the Ax 20 group (P < 0.01). No significant changes occurred in flavor attribute scores of meat soup from the Ax 20 group compared with the control even though most assessors preferred meat soup from the Ax 20 group. Overall, Ax‐rich yeast in the diet improves broiler chicken meat quality.  相似文献   

Lethal effects of nonylphenol on fertilized eggs and larvae of marbled sole Pleuronectes yokohamae     

Gen Kume  Toshihiro Horiguchi  Akihiro Goto  Tomohiko Isobe  Shigeko Serizawa  Hiroaki Shiraishi  Masatoshi Morita 《Fisheries Science》2006,72(1):217-219

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如何做好机降扑火的安全工作     

班泉  周兆木  秦光耀 《森林防火》2006,(4):39-40

针对机降扑火过程中存在的不安全隐患,结合多年在机降扑火过程中总结的经验,详细阐述了应注意事项及要求。  相似文献   

中国白兔生长发育特性的研究   总被引：1，自引：1，他引：0  

肖超能  班兆候 《中国养兔杂志》1996,(4):26-28

本研究对初生、２１、２８、３０、６０、９０、１２０、１５０、１８０日龄和１２月龄等不同阶段中国白兔的增重、成活率和繁殖性能进行测定，结果如下：窝均产仔８．０７只，初生产４９．１６克，２８日龄断奶产２８５．９６克，断奶成活率９４．９２％；９０日龄产９７１．１０克，成活率８２．２２％；１８０日龄产１７３２．７８克，成活率９１．２２％；成年体重２２７３．３３克。结果显示：中国白兔成年体重小，生长速度较慢，但繁殖能力强，成活率高  相似文献   

Evaluating maize growth models “CERES-Maize” and “IXIM-Maize” under elevated temperature conditions     

Ho-Young Ban  Dana Sim  Kyu-Jong Lee  Junhwan Kim  Kwang Soo Kim  Byun-Woo Lee 《Journal of Crop Science and Biotechnology》2015,18(4):265-272

It would be preferable to use a reliable crop growth model for studies on climate change impact assessment. The objectives of this study was to evaluate simulation performance for two maize models, including CERES-Maize and IXIM models, included in the DSSAT model (version 4.6) in terms of phenology and yield. Two early maturing cultivars, Chalok#1 and Junda# 6, were grown under controlled environment in plastic houses at Suwon, Korea. Each cultivar, which was sown at four different date in 2013 and 2014, was subjected to four sets of temperature conditions including ambient (AT), AT+1.5°C, AT+3°C, and AT+5°C. In simulations of phenology under given conditions, the anthesis date and grain filling ratio were underestimated, especially when temperature was unusually high, e.g., in 2013. The maize models also had poor accuracy in grain yield, which resulted from the fact that these models had relatively large errors in simulation of kernel number and kernel weight under elevated temperature conditions. In addition, both models were not able to simulate the drastic decrease of kernel number due to heat stress around flowering periods. These results indicated that two maize models would need improvements in simulation of crop response to supra-optimal temperature before they would be used to assess the impact of the climate change on maize yield. This studies merits further study to improve algorithms in phenology simulation at supraoptimal temperature.  相似文献   

Evaluation of resistance to Fusarium head blight in indigenous Japanese species of Agropyron (Elymus)   总被引：2，自引：0，他引：2  

Tomohiro Ban 《Euphytica》1997,95(1):39-44

General (GSI) and specific (SSI) selection indices were constructed for three open-mating populations of sugarcane (Saccharum spp.) seedlings and their ratoon stages. Though specific indices were found to be the most effective in improving mean brix yield in their own source populations, but the GSI was better to SSI in other populations as indicated by higher mean values and more number of superior clones at selection and evaluation stages. This indicated the wider applicability of the GSI over SSI. Inspite of better expected genetic advance through GSI in ratoon crop, the actual gain in brix yield attained, through GSI, was better in seedlings. This revised version was published online in July 2006 with corrections to the Cover Date.  相似文献   

Phosphorus-use efficiency of kale genotypes from coastal Croatia     

Branimir Urlić  Gvozden Dumičić  Smiljana Goreta Ban  Marija Romić 《Journal of plant nutrition》2016,39(3):389-398

Understanding the mechanisms of phosphorus (P)-use efficiency (PUE) may contribute to enhancing crop P nutrition because species growth variability at low-P is well known. The experiment was carried out to evaluate the response of kale genotypes to different P supply in randomized block design in three replications. Low-P supply led to a decrease in most parameters, whereas an increase was recorded in root growth parameters. Genotypes differed in shoot dry weight (DW), leaf area, root length and area, and shoot and root P content and concentration. Root traits significantly positively correlated with PUE. Genotypes Red Russian (RR) and IJK 17 were superior in terms of shoot DW production at low P supply, and had the highest uptake efficiency. Genotypes IJK 17 and 81 had the highest P utilization efficiency, while Vates blue curled (VBC) showed the lowest PUE. Genotypes had similar shoot P content and concentration at low P supply, but large PUE differences, implying the importance of P utilization efficiency.  相似文献