狂犬病病毒CVS-11株的培养及其效价测定     

张传明  冷雪梅  蒋雯雯  杨敏 《现代畜牧兽医》2014,(7):42-44

本研究通过神经瘤细胞（NA）培养CVS-11株狂犬病病毒,并测定不同批次的病毒毒价,为优化狂犬病疫苗的生产工艺提供数据支持,具有实际意义。病毒培养和检测结果表明,第1次和第2次收获的病毒液病毒含量较高,在107.0FFU/0.1 mL以上,毒力为106.4～107.0LD50/0.03 mL;而第3次收获的病毒含量则较低,只有106.0FFU/0.1 mL左右,毒力为105.0～105.3LD50/0.03 mL。第1次和第2次收获的病毒液可用于制备狂犬疫苗,第3次收获的病毒液因毒价较低不适于制备狂犬疫苗。  相似文献   

反刍动物饲料产品和动物源性饲料产品中牛羊源成分检测     

高延玲  狄元冉  董鹏  李金磊  张发旺  班付国 《畜牧与饲料科学》2014,(7):29-31

[目的]检测反刍动物饲料和动物源性饲料中牛羊源成分。[方法]利用实时荧光定量PCR技术对反刍动物饲料产品（预混料、精料补充料、全价配合料等）和动物源性饲料产品（鱼粉、禽肉粉、猪肉粉、羽毛粉等）共计131批样品进行了牛羊源成分检测。[结果]所有样品中均未检出牛羊源性成分。[结论]该次抽检的反刍动物饲料产品和动物源性饲料产品中暂无牛羊源成分污染,但仍应加强对反刍动物饲料和动物源性饲料中牛羊源成分的监测。  相似文献   

Comparative analysis of proteomic profiles between endometrial caruncular and intercaruncular areas in ewes during the peri-implantation period     

Yang Wang  Chao Wang  Zhuocheng Hou  Kai Miao  Haichao Zhao  Rui Wang  Min Guo  Zhonghong Wu  Jianhui Tian  Lei An 《畜牧与生物技术杂志(英文版)》2014,(1):53-63

The endometrium of sheep consists of plenty of raised intercaruncular areas （IC）. In order to better understand aglandular areas called caruncular （C）, and intensely glandular the endometrium involved mechanisms of implantation, we used LC-MS/MS technique to profile the proteome of ovine endometrial C areas and IC areas separately during the peri-implantation period, and then compared the proteomic profiles between these two areas. We successfully detected 1740 and 1813 proteins in C areas and IC areas respectively. By comparing the proteome of these two areas, we found 170 differentially expressed proteins （DEPs） （P 〈 0.05）, functional bioinformatics analysis showed these DEPs were mainly involved in growth and remodeling of endometrial tissue, cell adhesion and protein transport, and so on Our study, for the first time, provided a proteomic reference for elucidating the differences between C and IC areas, as an integrated function unit respectively, during the peri-implantation period. The results could help us to better understand the implantation in the ewes. In addition, we established a relatively detailed protein database of ovine endometrium, which provide a unique reference for further studies.  相似文献   

日本血吸虫四跨膜孤儿受体(TOR)克隆及其第一个膜外区基因的表达     

马帅  韩艳辉  洪炀  张旻  曹晓丹  韩倩  刘艳涛  陆看  马茜茜  陆珂  贾秉光  林矫矫  傅志强 《中国动物传染病学报》2014,(6):46-52

埃及血吸虫和曼氏血吸虫四跨膜孤儿受体(trispanning orphan receptors,TOR)受体可以结合补体C2a,可能在血吸虫免疫逃避过程中起重要作用。为研究日本血吸虫TOR受体的特性和功能,利用PCR扩增到Sj TOR基因并进行了生物信息学分析;同时克隆了Sj TOR基因N端第一个膜外区(ed1)基因,构建了重组质粒p ET-28a-Sj TOR-ed1,在大肠杆菌BL21(DE3)中诱导表达后用组蛋白亲和层析纯化获得重组蛋白Sj TOR-ed1,Western blot结果显示r Sj TOR-ed1能被日本血吸虫阳性小鼠血清识别。该研究为进一步开展研究日本血吸虫TOR受体的功能提供了基础。  相似文献   

Effects of dietary tryptophan and stocking density on the performance,meat quality,and metabolic status of broilers     

Bo Wang  Zhizhi Min  Jianmin Yuan  Bingkun Zhang  Yuming Guo 《畜牧与生物技术杂志(英文版)》2014,5(1):44

Background

Highly automated cage-rearing systems are becoming increasingly popular in China. However, a high stocking density can cause oxidative stress and decrease broiler performance. The tryptophan (TRP) derivative 5-hydroxytryptophan (5-HT) has been shown to preserve membrane fluidity in birds suffering from oxidative stress. Therefore, this experiment was conducted to determine the effects of dietary TRP supplementation on performance, breast meat quality and oxidative stress in broilers reared in cages with a high or low stocking density.

Methods

Female Arbor Acres broilers (25-d-old, n = 144) were randomly allocated to 1 of 4 treatments. The birds were fed a diet based on corn, soybean meal, cottonseed meal and corn gluten meal containing either 0.18 or 0.27% TRP and were housed with stocking densities of 11 or 15.4 birds/m² in a 2 × 2 factorial experiment. Broiler performance was evaluated from d 25 to 42. Eight birds from each treatment were slaughtered on d 42 and plasma and breast muscle samples were collected to measure biochemical indices.

Results

A higher stocking density tended to be associated with reduced weight gain (P < 0.10), and significantly increased plasma glutamic-pyruvic transaminase (GPT) activity (P < 0.001). Increased dietary TRP significantly reduced the activities of lactic dehydrogenase and GPT while increasing total cholesterol in the plasma (P < 0.01), reducing drip loss of breast muscle (P < 0.10) and improving feed efficiency (P < 0.10).

Conclusions

An increase in dietary TRP, 1.5-fold higher than the standard supplementation level, can alleviate oxidative stress as well as improve welfare and feed efficiency in broilers reared in cages with a high stocking density.  相似文献   

Novel human H7N9 influenza virus in China     

Chengmin WANG  Jing LUO  Jing WANG  Wen SU  Shanshan GAO  Min ZHANG  Li XIE  Hua DING  Shelan LIU  Xiaodong LIU  Yu CHEN  Yaxiong JIA  Hongxuan HE 《Integrative zoology》2014,9(3):372-375

Outbreaks of H7N9 avian influenza in humans in 5 provinces and 2 municipalities of China have reawakened concern that avian influenza viruses may again cross species barriers to infect the human population and thereby initiate a new influenza pandemic. Evolutionary analysis shows that human H7N9 influenza viruses originated from the H9N2, H7N3 and H11N9 avian viruses, and that it is as a novel reassortment influenza virus. This article reviews current knowledge on 11 subtypes of influenza A virus from human which can cause human infections.  相似文献   

鳜嗜水气单胞菌GYK1株胞外产物提取、纯化及生物学活性分析   总被引：2，自引：0，他引：2  

潘厚军  石存斌  刘瑞明  胡学峰  张敏  吴淑勤 《南方水产》2006,2(3):1-6

以鳜分离的嗜水气单胞菌GYK1株为代表,采用玻璃纸覆盖技术提取了8株嗜水气单胞菌和1株温和气单胞菌的胞外产物,SDS-PAGE电泳分析显示,8株嗜水气单胞菌的胞外产物均有35kDa的共同蛋白带。酶活性和溶血性分析表明,GYK1株胞外产物具有酪蛋白酶、淀粉酶、脂肪酶、明胶酶活性,但不具备脲酶活性;溶血性较强,蛋白浓度为365μg·mL-1的胞外产物对鳜血细胞的溶血价为213,对加州鲈、银鲫、小白鼠、兔、绵羊、人O型血的红细胞的溶血价在211~214之间。应用聚丙烯酰胺葡聚糖凝胶柱层析,对GYK1株的胞外产物进行了初步纯化,得到分子量为35kDa的蛋白带,该纯化产物具溶血性,但溶血性比粗胞外产物降低,对鳜和小鼠血细胞的溶血价均为25。  相似文献   

鲮鱼、麦瑞加拉鲮鱼和露斯塔野鲮的染色体组型比较   总被引：2，自引：0，他引：2  

邬国民  陈焜慈  罗建仁  冼炽彬  明朗 《水产学报》1989,13(3):259-263

近年来,人们对维科染色体的研究报道甚多,认为其染色体的基本二倍数为2N=50和2N=48,变异范围在2N=44～200之间。Gold等认为北美洲已见核型报道的绝大多数鱼类为2N=50。但欧亚大陆大多数为2N=48～50。Arai对欧亚大陆已研究过核型的141种鲤科鱼进行了分析,提出2N=50是鲤科鱼最原始的核型。周暾等对中国鲤科核型的研究也做了大量的工作并得出与Arai一致的结论。  相似文献   

中国鳀科鱼类一新属一新种          下载免费PDF全文

彭育斌  赵振伦 《水产学报》1988,12(4):355-357

本文报导了鳀科鱼类1新属1新种，定名为拟黄鲫属Pseuosetipinna，海州拟黄鲫Pseudosetipinna haizhouensis。  相似文献   

Characterization of the DNA nucleotide sequences in the genome of red sea bream iridoviruses isolated in Korea     

Joon Bum Jeong  Lyu Jin Jun  Min Ho Yoo  Myong Sug Kim  Jack L. Komisar  Hyun Do Jeong   《Aquaculture (Amsterdam, Netherlands)》2003,220(1-4):119-133

The nucleotide sequences of DNA fragments amplified by polymerase chain reaction (PCR) from four different genomic regions of nine red sea bream iridoviruses (RSIVs) isolated from different species of fish, different areas and in different years in Korea were compared with the reported reference sequences. One isolate, RSIV Namhae, showed 100% homology to the reference sequences, while the other eight isolates, which appeared to contain identical nucleotide sequences, showed 96.6–98.9% homology with reference sequences depending upon the target regions of PCR gene amplification. However, differences in nucleotide sequences were not apparent between the RSIVs isolated in different locations, in different years or in different host species. We also cloned and sequenced the 3′ end flanking region (K1) of the DNA polymerase (DPOL) gene using the cassette ligation-mediated PCR method. This sequence was 4436-bp long and possessed two open reading frames (ORF-1 and ORF-2) oriented in opposite directions. The putative proteins encoded by these two ORFs could not be characterized by comparison with the proteins of other species in the data banks. The presence of the ribonucleotide reductase small subunit (RNRS) gene at the 3′ end of the K1 region allowed us to determine that these two genes, RNRS and DPOL, are separated 5508 bp and oriented in the same direction in the genome of RSIV. Moreover, it is of interest that a PstI-restriction fragment, of which the sequence but not the location within the RSIV genome had previously been reported, is located at nucleotide positions from 1096 to 2054, extending from within the ORF-1 region, spanning the intervening sequence between ORF-1 and ORF-2, and extending into the ORF-2 region. Various repeating sequences up to 86 bp were present at the 3′ ends of ORFs, especially within the nucleotide sequences at the 3′ terminus of ORF-2. No similarities were detected when the DNA sequences of the K1 region were compared to the DNA sequences of a repetitive element in the genome of other iridoviruses.  相似文献