Methanotrophy-driven accumulation of organic carbon in four paddy soils of Bangladesh     

Nasrin SULTANA  Jun ZHAO  Yuanfeng CAI  G. K. M. Mustafizur RAHMAN  Mohammad Saiful ALAM  Mohammad FAHEEM  Adrian HO  Zhongjun JIA 《土壤圈》2022,32(2):348-358

Biological methane oxidation is a crucial process in the global carbon cycle that reduces methane emissions from paddy fields and natural wetlands into the atmosphere.However,soil organic carbon accumulation associated with microbial methane oxidation is poorly understood.Therefore,to investigate methane-derived carbon incorporation into soil organic matter,paddy soils originated from different parent materials(Inceptisol,Entisol,and Alfisol) were collected after rice harvesting from four major rice-producing regions in Bangladesh.Following microcosm incubation with 5%(volume/volume)¹³ CH₄,soil¹³ C-atom abundances significantly increased from background level of 1.08% to 1.88%–2.78%,leading to a net methane-derived accumulation of soil organic carbon ranging from 120 to 307 mg kg^-1.Approximately 23.6%–60.0% of the methane consumed was converted to soil organic carbon during microbial methane oxidation.The phylogeny of¹³ C-labeled pmoA(enconding the alpha subunit of the particulate methane monooxygenase) and 16 S rRNA genes further revealed that canonical α(type II) and γ(type I) Proteobacteria were active methane oxidizers.Members within the Methylobacter-and Methylosarcina-affiliated type Ia lineages dominated active methane-oxidizing communities that were responsible for the majority of methane-derived carbon accumulation in all three paddy soils,while Methylocystis-affiliated type IIa lineage was the key contributor in one paddy soil of Inceptisol origin.These results suggest that methanotroph-mediated synthesis of biomass plays an important role in soil organic matter accumulation.This study thus supports the concept that methanotrophs not only consume the greenhouse gas methane but also serve as a key biotic factor in maintaining soil fertility.  相似文献   

HYDRUS simulations of the effects of dual-drip subsurface irrigation and a physical barrier on water movement and solute transport in soils   总被引：2，自引：1，他引：1  

Mohammad N. El-Nesr  A. A. Alazba  Jiří Šimůnek 《Irrigation Science》2014,32(2):111-125

Subsurface drip irrigation systems, compared to other irrigation systems, enhance the delivery of water and nutrients directly into the root zone. However, in light-textured soils, certain quantities of water may percolate below the root zone due to the subsurface position of drip lines and/or poor management of irrigation systems. The main objective of this paper is to evaluate three technologies to enhance a spatial distribution of water and solutes in the root zone and to limit downward leaching. The three technologies include (a) a physical barrier, (b) a dual-drip system with concurrent irrigation, and (c) a dual-drip system with sequential irrigation. To achieve this objective, we performed computer simulations using the HYDRUS (2D/3D) software for both bare and vegetated soils. The results indicate that the physical barrier is more efficient than dual-drip systems in enhancing the water distribution in the root zone while preventing downward leaching. On the other hand, the dual-drip system improves water distribution in sandy soils. Additionally, the dual-drip system with sequential irrigation, followed by the dual-drip system with concurrent irrigation, is the most efficient in limiting downward leaching of solutes.  相似文献   

Electrochemical Production of Ferrate (Iron VI): Application to the Wastewater Treatment on a Laboratory Scale and Comparison with Iron (III) Coagulant     

Cécile Stanford  Jia-Qian Jiang  Mohammad Alsheyab 《Water, air, and soil pollution》2010,209(1-4):483-488

This paper presents a comparative study of the performance of ferrate(VI), FeO ₄ ^2? , and ferric, Fe(III), towards wastewater treatment. The ferrate(VI) was produced by electrochemical synthesis, using steel electrodes in a 16 M NaOH solution. Domestic wastewater collected from Hailsham North Wastewater Treatment Works was treated with ferrate(VI) and ferric sulphate (Fe(III)). Samples were analysed for suspended solids, chemical oxygen demand (COD), biochemical oxygen demand (BOD) and P removal. Results for low doses of Fe(VI) were validated via a reproducibility study. Removal of phosphorous reached 40% with a Fe(VI) dose as low as 0.01 mg/L compared to 25% removal with 10 mg/L of Fe(III). For lower doses (<1 mg/L as Fe), Fe(VI) can achieve between 60% and 80% removals of SS and COD, but Fe(III) performed even not as well as the control sample where no iron chemical was dosed. The ferrate solution was found to be stable for a maximum of 50 min, beyond which Fe(VI) is reduced to less oxidant species. This provided the maximum allowed storage time of the electrochemically produced ferrate(VI) solution. Results demonstrated that low addition of ferrate(VI) leads to good removal of P, BOD, COD and suspended solids from wastewater compared to ferric addition and further studies could bring an optimisation of the dosage and treatment.  相似文献   

Effects of triploidy on the Caspian salmon <Emphasis Type="Italic">Salmo trutta caspius</Emphasis> haematology     

Dorafshan S  Kalbassi MR  Pourkazemi M  Amiri BM  Karimi SS 《Fish physiology and biochemistry》2008,34(3):195-200

The aim of this study was a comparison of key haematological features of diploid (2n) and triploid (3n) Caspian salmon (Salmo trutta caspius). Morphometric indices of erythrocytes were determined on blood smears by light microscopy. Triploidy significantly (P < 0.001) increased all morphometric indices measured in the erythrocytes including cell size, cell surface area, and cell volume. The increase in cell size was larger for the major (27%) axis than for the minor (22%) axis, thus making erythrocytes of 3n Caspian salmon more ellipsoidal. The estimated increase in erythrocyte nuclear volume (87%) was bigger than the theoretical expected 50% increase. Haematological indices were measured manually by hemocytometry. Triploids had lower numbers of red blood cells (RBC: 1,120,000 cells/mL in 2n vs. 700,000 cells/mL in 3n; P < 0.001) but they were larger in size (mean erythrocytic volume [MEV]: 363.1 nm³ in 2n vs. 483.3 nm³ in 3n; P < 0.001). The decrease in RBC number was not compensated by the increase in MEV and, thus, triploidy affected the haematocrit (Hct: 38.8% in 2n vs. 33.06% in 3n; P < 0.05). Total blood hemoglobin concentration was lower in triploid fish (Hb: 9.9 g/dL in 2n vs. 8.9 g/dL in 3n; P < 0.05). In contrast, mean erythrocytic hemoglobin (MEH: 95 μg in 2n vs. 133.2 μg in 3n; P < 0.001) was higher for 3n Caspian salmon as a result of their larger erythrocytes, although MEH concentration (MEHC: 0.26 g/dL in 2n vs. 0.27 g/dL in 3n) did not significantly differ (P > 0.05). White blood cell (WBC) counts (lymphocytes and neutrophiles) were measured and WBC/RBC ratios were calculated. There were no significant differences in WBC (15,710 cells/mL in 2n vs. 12,683 cells/mL in 3n; P > 0.05), lymphocytes, and neutrophils as %WBC as well as WBC/RBC ratios between two ploidy levels (P > 0.05). Triploid Caspian salmon showed higher erythrocyte abnormalities such as ‘twisted’, ‘tailed’, and ‘anucleated’ cells as well as high portions of immature RBC in blood smears in comparison with diploids (P < 0.001).  相似文献   

Efficiency of some molecular markers linked to rhizomania resistance gene (Rz 1 ) for marker assisted selection in sugar beet     

Peyman Norouzi  Mohammad Sabzehzari  Hasan Zeinali 《Journal of Crop Science and Biotechnology》2015,18(5):319-323

Sugar beet (Beta vulgaris L.) is one of the two major products supplying sugar (sucroses) in the world. Rhizomania is one of the most destructive diseases of sugar beet world-wide. Holly is the major source of resistance to rhizomania. The objectives of this study were to identify the dominant homozygous genotypes resistant to rhizomania using ZN1 molecular marker, to field evaluate S₁ progenies of plants already proved to be containing the marker and also to determine the relationship of this and other SCAR (sequence characterized amplified region) markers with SNP1 (single nucleotide polymorphism) marker associated with the Rz ₁ gene. Molecular analysis was carried out on 27 O-type populations (consisting of 13 susceptible and 6 resistant genotypes). Field evaluation and scoring of the phenotypic traits including greenness, growth, uniformity and disease score of 12 O-type populations were carried out on a rhizomania-infested field. The percent agreement of coupling marker ZN1 and repulsion marker ZN8 with disease score was 0.91 and 0.93, respectively. Although all O-types had the Rz ₁ resistance gene but the phenotypic differences were observed due to the effect of different genetic backgrounds and modifier genes. Overall, the results showed that the selected markers can be used for marker-assisted selection (MAS) to reduce the time and cost of breeding programs and increase the efficiency of selection.  相似文献   

Aging of whiskey increases the potentiation of GABA(A) receptor response     

Koda H  Hossain SJ  Kiso Y  Aoshima H 《Journal of agricultural and food chemistry》2003,51(18):5238-5244

It is known that the target of most mood-defining compounds such as ethanol is an ionotropic gamma-aminobutyric acid receptor (GABA(A) receptor). The potentiation of the response of these inhibitory neurotransmitter receptors induces anxiolytic, sedative, and anesthetic activities in the human brain. Because both extracts of whiskey by pentane and fragrant components in whiskey potentiate the GABA(A) receptor-mediated response, GABA(A) receptors were expressed in Xenopus oocyte by injecting cRNAs prepared from the cloned cDNA for the alpha(1) and beta(1) subunits of the bovine receptors in order to study the effects of whiskey itself on the GABA(A) receptor-mediated response. Whiskey itself also potentiated the electrical responses of GABA(A) receptors generally more than ethanol at the same concentration as that of the whiskey. The potentiation of the GABA(A) receptor-mediated response increased with the aging period of the whiskey. Inhalation of whiskey to mice increased the sleeping time induced by pentobarbital more than that of the same concentration of ethanol as the whiskey. These results suggest that not only ethanol but also minor components in whiskey play an important role in the potentiation of GABA(A) receptor-mediated response and possibly the sedative effect of whiskey. Although the minor components are present in extremely small quantities compared with ethanol in alcoholic beverages, they may modulate the mood or consciousness of humans through the potentiation of the GABA(A) receptor response after absorption into the brain, because these hydrophobic compounds are easily absorbed into the brain across the blood-brain barrier and are several thousands times as potent as ethanol in the potentiation of the GABA(A) receptor-mediated response.  相似文献   

Effects of coffee components on the response of GABA(A) receptors expressed in Xenopus oocytes     

Hossain SJ  Aoshima H  Koda H  Kiso Y 《Journal of agricultural and food chemistry》2003,51(26):7568-7575

The effects of both coffee components and coffee extract on the electrical responses of GABA(A) receptors expressed in Xenopus oocytes were studied by injecting cRNAs of the alpha(1) and beta(1) subunits of the bovine receptors. The aqueous extract of coffee dose-dependently inhibited the GABA-elicited responses, whereas the lipophilic extract of coffee by diethyl ether slightly potentiated it at low doses (0.1-0.4 microL/mL) but showed inhibition at high doses (0.5-0.8 microL/mL). Theophylline inhibited the response in a noncompetitive mechanism (K(i) = 0.55 mM), whereas theobromine and trigonelline hydrochloride inhibited it in a competitive manner, K(i) = 3.8 and 13 mM, respectively. Benzothiazole, catechol, 2,4-dimethylstyrene, guaiacol, 1-octen-3-ol, sotolone, and 2,3,5-trimethylphenol potentiated the responses significantly. Potentiation elicited by guaiacol and sotolone was independent of GABA concentrations, whereas that by 1-octen-3-ol was dependent. When 1-octen-3-ol (100 mg/kg) was orally administered to mice prior to intraperitoneal administration of pentobarbital, the sleeping time of mice induced by pentobarbital increased significantly.  相似文献   

Proton toxicity interferes with the screening of common bean (Phaseolus vulgaris L.) genotypes for aluminium resistance in nutrient solution     

Andrs F. Rangel  Mohammad Mobin  Idupulapati M. Rao  Walter J. Horst 《植物养料与土壤学杂志》2005,168(4):607-616

Common bean (Phaseolus vulgaris L.) proved to be very sensitive of low pH (4.3), with large genotypic differences in proton sensitivity. Therefore, proton toxicity did not allow the screening of common bean genotypes for aluminium (Al) resistance using the established protocol for maize (0.5 mM CaCl₂, 8 μM H₃BO₃, pH 4.3). Increasing the pH to 4.5, the Ca²⁺ concentration to 5 mM, and addition of 0.5 mM KCl fully prevented proton toxicity in 28 tested genotypes and allowed to identify differences in Al resistance using the inhibition of root elongation by 20 μM Al supply for 36 h as parameter of Al injury. As in maize, Al treatment induced callose formation in root apices of common bean. Aluminium‐induced callose formation well reflected the effect of Ca supply on Al sensitivity as revealed by root‐growth inhibition. Aluminum‐induced callose formation in root apices of 28 bean genotypes differing in Al resistance after 36 h Al treatment was positively correlated to Al‐induced inhibition of root elongation and Al contents in the root apices. However, the relationship was less close than previously reported for maize. Also, after 12 h Al treatment, callose formation and Al contents in root apices did not reflect differences in Al resistance between two contrasting genotypes, indicating a different mode of the expression of Al toxicity and regulation of Al resistance in common bean than in maize.  相似文献   

Nitrogen fertilizer–induced mineralization of soil organic C and N in six contrasting soils of Bangladesh     

Mohammad I. Khalil  Muhammad S. Rahman  Urs Schmidhalter  Hans‐Werner Olfs 《植物养料与土壤学杂志》2007,170(2):210-218

A 90‐day laboratory incubation study was carried out using six contrasting subtropical soils (calcareous, peat, saline, noncalcareous, terrace, and acid sulfate) from Bangladesh. A control treatment without nitrogen (N) application was compared with treatments where urea, ammonium sulfate (AS), and ammonium nitrate (AN) were applied at a rate of 100 mg N (kg soil)^–1. To study the effect of N fertilizers on soil carbon (C) turnover, the CO₂‐C flux was determined at nine sampling dates during the incubation, and the total loss of soil carbon (TC) was calculated. Nitrogen turnover was characterized by measuring net nitrogen mineralization (NNM) and net nitrification (NN). Simple and stepwise multiple regressions were calculated between CO₂‐C flux, TC, NNM, and NN on the one hand and selected soil properties (organic C, total N, C : N ratio, CEC, pH, clay and sand content) on the other hand. In general, CO₂‐C fluxes were clearly higher during the first 2 weeks of the incubation compared to the later phases. Soils with high pH and/or indigenous C displayed the highest CO₂‐C flux. However, soils having low C levels (i.e., calcareous and terrace soils) displayed a large relative TC loss (up to 22.3%) and the added N–induced TC loss from these soils reached a maximum of 10.6%. Loss of TC differed depending on the N treatments (urea > AS > AN >> control). Significantly higher NNM was found in the acidic soils (terrace and acid sulfate). On average, NNM after urea application was higher than for AS and AN (80.3 vs. 71.9 and 70.9 N (kg soil)^–1, respectively). However, specific interactions between N‐fertilizer form and soil type have to be taken into consideration. High pH soils displayed larger NN (75.9–98.1 mg N (kg soil)^–1) than low pH soils. Averaged over the six soils, NN after application of urea and AS (83.3 and 82.2 mg N (kg soil)^–1, respectively) was significantly higher than after application of AN (60.6 mg N (kg soil)^–1). Significant relationships were found between total CO₂ flux and certain soil properties (organic C, total N, CEC, clay and sand content). The most important soil property for NNM as well as NN was soil pH, showing a correlation coefficient of –0.33** and 0.45***, respectively. The results indicate that application of urea to acidic soils and AS to high‐pH soils could be an effective measure to improve the availability of added N for crop uptake.  相似文献   

Effects of vermicompost and salinity stress on growth and physiological traits of Medicago rigidula L.     

Davoud Akhzari  Mohammad Pessarakli  Majid Khedmati 《Journal of plant nutrition》2016,39(14):2106-2114

The use of vermicompost as a biological fertilizer under salinity stress conditions was tested in this research. Accordingly, the seeds of Medicago rigidula were grown in the greenhouse. The experiment was performed based on factorial arrangement in a completely randomized design using 5 replications. Application rates of vermicompost were 0, 10, 20 and 30%. Salinity stress was conducted in three levels (0, 50 and 100 mM sodium chloride (NaCl)). The highest and the lowest values of the plant survival capacity (%), shoot dry weight (g), leaf relative water content (LRWC) (%), total chlorophyll content (%), leaf area (cm²), total nitrogen content (TNC) (%) and potassium (K) content (%) of the plant tissues were found in VC4 × SL1 and VC1 × SL3 treatments, respectively. Whereas, the maximum and minimum values of root dry weight and root:shoot ratio were seen in VC1 × SL3 and VC4 × SL1 treatments, respectively.  相似文献