Cloned porcine embryos can maintain developmental ability after cryopreservation at the morula stage     

Nakano K  Matsunari H  Nakayama N  Ogawa B  Kurome M  Takahashi M  Matsumoto M  Murakami H  Kaji Y  Nagashima H 《The Journal of reproduction and development》2011,57(2):312-316

The aim of the present study was to clarify the overall efficiency of porcine somatic cell nuclear transfer (SCNT) by incorporating cryopreservation of the cloned embryos before transfer. The SCNT embryos reconstructed with preadipocytes and in vitro-matured (IVM) oocytes were cultured to harvest morula stage embryos; they were then subjected to delipation (removal of cytoplasmic lipid droplets) and vitrification. After warming and culture, the embryos developing to blastocysts were transferred to recipients to obtain cloned piglets. From 372 reconstructed embryos, 188 (50.5%) reached the morula stage and 117 (31.5%) developed to blastocysts after vitrification. Transfer of 98 (26.3%) morphologically normal blastocysts gave rise to 6 (1.6%) piglets, including 1 stillborn. The efficiency of the cloned piglet production was comparable with that obtained using SCNT embryos without cryopreservation (2.7%, 17/635). Here, we demonstrate that porcine somatic cell cloning can be performed without a significant reduction in efficiency even when the SCNT embryos are cryopreserved before transfer.  相似文献   

Fecal progestagens to detect and monitor pregnancy in captive female cheetahs (Acinonyx jubatus)     

Adachi I  Kusuda S  Kawai H  Ohazama M  Taniguchi A  Kondo N  Yoshihara M  Okuda R  Ishikawa T  Kanda I  Doi O 《The Journal of reproduction and development》2011,57(2):262-266

The purposes of the present study were to establish a noninvasive monitoring assay of fecal progestagen measurement to detect pregnancy and to identify the components of fecal progestagens in early, middle and late pregnancy in cheetahs. Feces were collected from 7 female cheetahs and analyzed from 30 days before the last copulation to parturition in 9 pregnancies. Blood was collected from one cheetah. Fecal progestagen and serum progesterone concentrations were determined by enzyme immunoassay (EIA). The profiles of the fecal progestagen concentrations were similar to the serum progesterone profile. Fecal progestagen and serum progesterone concentrations remained at the baseline until copulation. In the mean fecal progestagen profile during pregnancy (92.8 ± 0.4 days; from the last copulation to parturition), the concentrations increased 3-4 days after the last copulation and remained high until parturition. To investigate changes in the components of progestagen metabolites in the tripartite periods of gestation, fecal progestagens were analyzed by HPLC-EIA. Marked immunoreactive peaks consistent with 5α-pregnan-3α/β-ol-20-one and 5α-pregnan-3,20-dione and small peaks consistent with 5β-pregnan-3α/β-ol-20-one were detected. There were no distinct difference in the components of progestagens among the first, second and third trimesters of pregnancy. The hormone assay, as an indicator of fecal 5α-reduced pregnanes, is useful for detecting pregnancy and monitoring pregnant luteal activity in cheetahs.  相似文献   

Evaluation of transplantation of autologous bone marrow stromal cells into the cerebrospinal fluid for treatment of chronic spinal cord injury in dogs     

Nishida H  Nakayama M  Tanaka H  Kitamura M  Hatoya S  Sugiura K  Suzuki Y  Ide C  Inaba T 《American journal of veterinary research》2011,72(8):1118-1123

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Expression of cell adhesion molecules and doublecortin in canine anaplastic meningiomas     

Ide T  Uchida K  Suzuki K  Kagawa Y  Nakayama H 《Veterinary pathology》2011,48(1):292-301

Tumor cell invasion into the surrounding nervous tissue is one of the histologic hallmarks of anaplastic meningiomas. To identify other possible markers for aggression in canine meningiomas, the relationship between histologic features and the expression of molecules involved in cell adhesion, cell proliferation, and invasion was examined. Immunohistochemistry for epithelial cadherin (E-cadherin), neural cadherin (N-cadherin), β-catenin, doublecortin (DCX), and Ki-67 was performed for 55 cases of canine meningioma. DCX was preferentially expressed in tumor cells invading the brain parenchyma (12 of 14 cases), suggesting its involvement in the invasion process. Regardless of the histologic type, E-cadherin and N-cadherin expression was observed in 31 of 55 and 44 of 55 cases, respectively. There was a significant positive correlation between DCX and N-cadherin expression and a significant negative correlation between E-cadherin and N-cadherin expression, suggesting that decreased E-cadherin and increased N-cadherin expression induce DCX expression. Typical membranous β-catenin expression was observed in 10 of 55 cases, whereas nuclear translocation was observed in 33 cases. Nuclear β-catenin expression was frequently found in anaplastic meningiomas (12 of 14 cases). The Ki-67 labeling indices were significantly higher in anaplastic meningiomas than in other types. These findings indicate that the expression of N-cadherin and DCX and the nuclear translocation of β-catenin are closely associated with the presence of invasion and anaplasia in canine meningiomas. Notably, granular cell meningiomas were negative for almost all the molecules examined, suggesting that they have a different tumor biology than other meningiomas.  相似文献   

Effects of tris(1,3-dichloro-2-propyl) phosphate on epididymal sperm parameters in adult male rats     

Shohei KOBAYASHI  Natsuko KAWANO  Kenji MIYADO  Ryo OHTA  Takahiro AKIMOTO  Taichi HATAKEYAMA  Maiko KAWAGUCHI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2022,84(1):153

Tris(1,3-dichloro-2-propyl) phosphate (TDCIPP) is widely used as a flame retardant and is known to exhibit anti-androgenic effects in vitro and in vivo. To assess the reproductive toxicity potency of TDCIPP, we investigated the effects of 7 days of TDCIPP oral administration on epididymal sperm motion and concentration in adult male Wistar–Imamichi rats. Thirty-five days after the final administration, sperm parameters were evaluated by computer-assisted sperm analysis. Results showed that sperm swimming progression and vigor and sperm concentration in TDCIPP-treated rats were unexpectedly higher than those in control rats. TDCIPP did not significantly affect the percentage of motile sperms or sperm swimming pattern. These results contribute to the understanding of the biological effects of TDCIPP.  相似文献   

Studies on availability and fixation of phosphorus in soil (part 4)     

Tadashi Nakayama  Takashi Yamashita 《Soil Science and Plant Nutrition》2013,59(5):128-132

It Is known that inorganic phosphorus is contained in soil mainly in the form of calcium phosphates, aluminium phosphates and iron phosphates.  相似文献   

Water quality in drip/trickle irrigation: A review   总被引：8，自引：2，他引：8  

F. S. Nakayama  D. A. Bucks 《Irrigation Science》1991,12(4):187-192

Summary The intensive treatment of irrigation water required for the proper operation of drip irrigation systems is presently an accepted practice. To control emitter clogging, we need to know the basic causes of clogging. The major clogging factors have been identified and control measures developed to prevent emitter malfunction. All emitter clogging problems, however, have not been solved primarily because of cost. The main approach to control clogging is proper water treatment. The type of treatment is based on the quality of the irrigation water, which can be classified in terms of its physical, chemical and biological composition. The causes of emitter clogging and possible water treatment and preventive measures to maintain reliable operation are reviewed.  相似文献   

Phenolic antioxidants from the leaves of Corchorus olitorius L.   总被引：3，自引：0，他引：3  

K Azuma  M Nakayama  M Koshioka  K Ippoushi  Y Yamaguchi  K Kohata  Y Yamauchi  H Ito  H Higashio 《Journal of agricultural and food chemistry》1999,47(10):3963-3966

Six phenolic antioxidative compounds [5-caffeoylquinic acid (chlorogenic acid), 3,5-dicaffeoylquinic acid, quercetin 3-galactoside, quercetin 3-glucoside, quercetin 3-(6-malonylglucoside), and quercetin 3-(6-malonylgalactoside) (tentative)] were identified from the leaves of Corchorus olitorius L. (moroheiya) by NMR and FAB-MS. The contents of these phenolic compounds, ascorbic acid, and alpha-tocopherol in C. olitorius leaves were determined, and their antioxidative activities were measured using the radical generator-initiated peroxidation of linoleic acid. The results obtained showed that 5-caffeoylquinic acid was a predominant phenolic antioxidant in C. olitorius leaves.  相似文献   

Morphological analysis of viral communities in the floodwater of a Japanese paddy field     

Natsuko Nakayama  Mami Okumura  Susumu Asakawa 《Soil biology & biochemistry》2007,39(12):3187-3190

Capsid size distributions of viral communities in the floodwater of a Japanese paddy field under a long-term fertilizer trial were surveyed during the rice cultivation period by using transmission electron microscopy. The capsid size distributions were monophasic, and the median values fell within the range of 50-70 nm. The quartile intervals were narrow from either 40-50 nm or 50-60 nm to either 60-70 nm or 70-80 nm for most samples. There was no clear seasonal variation in the capsid size distribution of viral communities. The difference in size distributions among different fertilizer plots was also not found. Viral communities in the floodwater were predominated by isometric icosahedral forms. Viruses with octahedral capsids and elongated ones were rare and sporadic in the floodwater.  相似文献   

Metabarcoding of feces and intestinal contents to determine carnivorous diets in red-crowned cranes in eastern Hokkaido,Japan     

Haruka KATAOKA  Nanami KOITA  Natsuko ITO KONDO  Hiroshi C. ITO  Momoko NAKAJIMA  Kunikazu MOMOSE  Hiroko IIMA  Tomoo YOSHINO  Tomoko AMANO  Takio KITAZAWA  Daiji ENDOH  Nobuyoshi NAKAJIMA  Hiroki TERAOKA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2022,84(3):358

The red-crowned crane Grus japonensis in Hokkaido, Japan forms a closed population as a residence that is independent of the mainland population. Based on observations of a limited number of individuals as well as cranes in captivity, red-crowned cranes are omnivores and eat fish, worms, insects and plants in their own territories except in winter, when they are fed with dent corn that is supplied in eastern Hokkaido. DNA metabarcoding based on high throughput sequencing was carried out using universal primer sets for cytochrome oxidase subunit I gene. Feces from 27 chicks collected in June and July in the period from 2016 to 2018 and intestinal contents from 33 adult and subadult cranes that were found dead almost throughout year in 2006–2013 in the field in eastern Hokkaido were used. Although compositions varied considerably in the cranes, both insects and fish were found in adults and subadults to the same extents, while insects were predominant in chicks. Both insects and fish were detected in all seasons for adults and subadults. Horse flies, scarab beetles and weevils accounted for the most of the insects regardless of the life stage. Dace, stickleback, flatfish and sculpin were the major fish species in adults, while chicks ate almost only stickleback. The results provide the first comprehensive data on carnivorous diets in wild red-crowned cranes in eastern Hokkaido as basis for conservation of red-crowned cranes, for which the life style and area continue to change.  相似文献