Hepatitis E virus chronic infection of swine co-infected with Porcine Reproductive and Respiratory Syndrome Virus     

Morgane Salines  Elodie Barnaud  Mathieu Andraud  Florent Eono  Patricia Renson  Olivier Bourry  Nicole Pavio  Nicolas Rose 《Veterinary research》2015,46(1)

In developed countries, most of hepatitis E human cases are of zoonotic origin. Swine is a major hepatitis E virus (HEV) reservoir and foodborne transmissions after pork product consumption have been described. The risk for HEV-containing pig livers at slaughter time is related to the age at infection and to the virus shedding duration. Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) is a virus that impairs the immune response; it is highly prevalent in pig production areas and suspected to influence HEV infection dynamics. The impact of PRRSV on the features of HEV infections was studied through an experimental HEV/PRRSV co-infection of specific-pathogen-free (SPF) pigs. The follow-up of the co-infected animals showed that HEV shedding was delayed by a factor of 1.9 in co-infected pigs compared to HEV-only infected pigs and specific immune response was delayed by a factor of 1.6. HEV shedding was significantly increased with co-infection and dramatically extended (48.6 versus 9.7 days for HEV only). The long-term HEV shedding was significantly correlated with the delayed humoral response in co-infected pigs. Direct transmission rate was estimated to be 4.7 times higher in case of co-infection than in HEV only infected pigs (0.70 and 0.15 per day respectively). HEV infection susceptibility was increased by a factor of 3.3, showing the major impact of PRRSV infection on HEV dynamics. Finally, HEV/PRRSV co-infection – frequently observed in pig herds – may lead to chronic HEV infection which may dramatically increase the risk of pig livers containing HEV at slaughter time.

Electronic supplementary material

The online version of this article (doi:10.1186/s13567-015-0207-y) contains supplementary material, which is available to authorized users.  相似文献   

Population pharmacokinetics of methadone hydrochloride after a single intramuscular administration in adult Japanese sika deer (Cervus nippon nippon)          下载免费PDF全文

Christopher Scala  Amélie Marsot  Marie‐Josée Limoges  Yann Locatelli  Nicolas Simon  Jean‐Claude Alvarez 《Veterinary anaesthesia and analgesia》2015,42(2):165-172

ObjectiveTo assess the population pharmacokinetics of methadone in deer.Study designProspective non-randomized experimental trial.AnimalsTwelve healthy adult sika deer (nine males and three females).MethodsDeer received intramuscular administration of racemic methadone hydrochloride at 0.5 mg kg⁻¹ or 1 mg kg⁻¹. Plasma methadone and its metabolite 2-Ethylidene-1,5-Dimethyl-3,3-Diphenyl-Pyrolidine (EDDP) concentrations were determined by validated liquid chromatography coupled to tandem mass spectrometry methods, at times 0, 30 minutes, 1, 2, 3, 4, 5, 6, 8, 12 and 24 hours. Population pharmacokinetics analysis was undertaken using a non-linear mixed effects modelling (NONMEM).ResultsA two-compartment linear disposition model best described observed time-concentration profiles of methadone and EDDP. Population parameter estimates of methadone were elimination clearance (17.3 L hour⁻¹), metabolic clearance (34.6 L hour⁻¹), volume of distribution of compartment 1 (216.0 L) and volume of distribution of compartment 2 (384.0 L). Population parameter estimates of EDDP were elimination clearance (121.0 L hour⁻¹), volume of distribution of compartment 3 (1.08 L) and volume of distribution of compartment 4 (499.5 L). The total clearance and total volume of distribution of methadone and EDDP were 51.9 L hour⁻¹, 121.0 L hour ⁻¹, 600.0 L and 500.6 L, respectively. The methadone terminal elimination half-life was 8.19 hours. No adverse effects were observed after methadone administration.Conclusions and Clinical relevanceFollowing intramuscular injection, methadone was characterized by a large total volume of distribution, high systemic clearance and intermediate terminal half-life in sika deer.  相似文献   

Methane emissions and δ13C composition from beef steers consuming increasing proportions of sericea lespedeza hay on bermudagrass hay diets     

Flavia O S van Cleef  Jos C B Dubeux  Jr.  Harley D Naumann  Erick R S Santos  Lynn E Sollenberger  Joo M B Vendramini  Martin Ruiz-Moreno  Francine M Ciriaco  Nicolas DiLorenzo 《Journal of animal science》2021,99(8)

An experiment was conducted to evaluate the effects of different proportions of ‘Au Grazer’ sericea lespedeza [SL, Lespedeza cuneata (Dum. Cours.) G. Don], a legume rich in condensed tannins (CT), on nutrient intake and digestibility, and to estimate methane (CH₄) emissions and ¹³C isotopic composition (δ¹³C_CH4) from beef steers consuming a forage-based diet. Twenty-five Angus-crossbred steers were distributed in a randomized complete block design (344 ± 48 kg initial BW), and randomly assigned to one of five treatments: 0SL, 25SL, 50SL, 75SL, and 100SL, diets containing 0%, 25%, 50%, 75%, and 100% of SL hay, respectively, mixed with ‘Tifton-85’ bermudagrass hay (Cynodon spp.). The study was carried out for two experimental periods of 21-d each. The statistical model included the fixed effect of treatment and random effects of block, experimental period, and their interaction. Apparent total tract digestibility of crude protein, neutral detergent fiber, and acid detergent fiber was linearly decreased (P < 0.001) by the inclusion of SL. No effects were observed for total CH₄ emissions per day, nor for CH₄ relative to organic matter intake or digestible organic matter with the inclusion of SL. However, emission of CH₄ in relation to intake of CT was affected by treatment (P < 0.001). A linear (P < 0.001) decrease and a quadratic effect (P < 0.001) were observed for δ¹³C of diets and gas, respectively, in which diets and enteric CH₄ with greater inclusion of SL were more depleted in ¹³C. Moreover, the difference in δ¹³C between diets and gas (Δδ¹³C) had a linear decrease (P = 0.001) with the inclusion of SL. The model developed to predict the C₃ proportions in the enteric CH₄ fitted to predicted values (P < 0.0001). Therefore, greater proportions of SL resulted in lesser CH₄ emission when CT intake was considered and the isotopic composition from enteric CH₄ was able to predict the contribution of SL in the emissions.  相似文献   

Early introduction of water and complementary feeding and nutritional status of children in northern Senegal     

Gupta N  Gehri M  Stettler N 《Public health nutrition》2007,10(11):1299-1304

OBJECTIVE: Malnutrition is responsible globally for 60% of deaths among children under 5 years and is often attributed to suboptimal feeding practices. In response, the World Health Organization recommends exclusive breast-feeding for the first 6 months of life. The objective of this study was to determine if an association exists between the early introduction of water and complementary foods (CFs) and the nutritional status of children in northern Senegal. DESIGN/SETTING/SUBJECTS: A cross-sectional study of 374 children in the Podor Health District between the ages of 6 and 23 months was conducted. Knowledge and behaviours of mothers regarding introduction of water and CFs were assessed via individual interviews. RESULTS: Water was introduced to about 85% of the children in the first 3 months of life and 62% were fed CFs before 6 months. Overall, 16% had clinically significant wasting (weight-for-length Z-score (WHZ) less than - 2) and 20% had stunting (height-for-age Z-score (HAZ) less than - 2). There was no significant association between wasting or stunting and introduction of water before 3 months (WHZ: odds ratio = 0.99, 95% confidence interval 0.46-2.14, P = 0.97; HAZ: 0.68, 0.34-1.36, P = 0.3) or introduction of CFs before 6 months (WHZ: 0.81, 0.46-1.42, P = 0.5; HAZ: 0.79, 0.46-1.35, P = 0.4). A significant association was found between wasting and male sex, age, living in Guede community, drinking river/pond water and large family size, while stunting was associated with age and drinking tap water. CONCLUSION: The results of the present study suggest that early introduction of water and CFs is frequent and is not associated with increased risk for malnutrition among children from this region of northern Senegal, but the possibility of reverse causality cannot be excluded.  相似文献   

Integrated pest management of Tuta absoluta: practical implementations across different world regions     

Desneux  Nicolas  Han  Peng  Mansour  Ramzi  Arn&#;  Judit  Br&#;vault  Thierry  Campos  Mateus R.  Chailleux  Anais  Guedes  Raul N. C.  Karimi  Javad  Konan  Kouassi Arthur J.  Lavoir  Anne-violette  Luna  Mar&#;a G.  Perez-Hedo  Meritxell  Urbaneja  Alberto  Verheggen  Fran&#;ois J.  Zappal&#;  Lucia  Abbes  Khaled  Ali  Abid  Bayram  Yunus  Cantor  Fernando  Cuthbertson  Andrew G. S.  De Vis  Raf  Erler  Fedai  Firake  Dnyaneshwar M.  Haddi  Khalid  Hajjar  M. Jamal  Ismoilov  Khasan  Jaworski  Coline C.  Kenis  Marc  Liu  Hao-tian  Madadi  Hossein  Martin  Thibaud  Mazih  Ahmed  J. Messelink  Gerben  Mohamed  Samira A.  Nofemela  Robert S.  Oke  Abiola  Ramos  C&#;sar  Ricupero  Michele  Roditakis  Emmanouil  Shashank  Pathour R.  Wan  Fang-Hao  Wang  Ming-hui  Wang  Su  Zhang  Yi-Bo  Biondi  Antonio 《Journal of pest science》2022,95(1):17-39

Journal of Pest Science - The South American tomato pinworm, Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae), has invaded most Afro-Eurasian countries and is threatening worldwide tomato...  相似文献   

Flower strips adjacent to greenhouses help reduce pest populations and insecticide applications inside organic commercial greenhouses     

Li  Shu  Jaworski  Coline C.  Hatt  Séverin  Zhang  Fan  Desneux  Nicolas  Wang  Su 《Journal of pest science》2021,94(3):679-689

Journal of Pest Science - Flower strips can play an important role in agro-ecosystems by supporting populations of pests’ natural enemies, thereby enhancing biological control. However, few...  相似文献   

Thermal biology of Tuta absoluta: demographic parameters and facultative diapause     

de Campos  Mateus Ribeiro  Béarez  Philippe  Amiens-Desneux  Edwige  Ponti  Luigi  Gutierrez  Andrew Paul  Biondi  Antonio  Adiga  Abhijin  Desneux  Nicolas 《Journal of pest science》2021,94(3):829-842

Journal of Pest Science - The South American tomato pinworm, Tuta absoluta, (SATP) is now a devastating pest worldwide of crops in the family Solanaceae. Most prior studies of SATP’s thermal...  相似文献   

Characterisation of camel breeding practices in the Ansongo Region,Mali     

Bakary Traoré  Nassim Moula  Abdoulaye Toure  Bara Ouologuem  Pascal Leroy  Nicolas Antoine-Moussiaux 《Tropical animal health and production》2014,46(7):1303-1312

Despite its importance in Mali’s economy, camel breeding in the country remains poorly documented, impeding effective policy-making in this regard. This study consisted in a 3-month survey and aimed at characterising camel breeding systems in Ansongo, in the region of Gao, Mali. It highlights the diversity of strategies adopted by breeders and their evolutions. Supplementary feeding and veterinary care were seldom practised. In zones close to the Niger River, cattle were substituted to camels. Transhumance routes also are modified but mobility keeps its vital role in the breeding system. Important differences within the study region in the classification of camel breeds have been reported that will influence the implementation of a collective action for animal genetic improvement. The improvement goals should take the actual management, including mobility and the mixed nature of the herds into account.  相似文献   

Effect of epinephrine and seawater turbulence on the metamorphosis of the great scallop     

L. Nicolas  R. Robert  L. Chevolot 《Aquaculture International》1996,4(3):293-297

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Intra-specific variation of wheat grain quality in response to elevated [CO₂] at two sowing times under rain-fed and irrigation treatments     

Nimesha Fernando  Joe Panozzo  Michael Tausz  Robert M. Norton  Glenn J. Fitzgerald  Samuel Myers  Marc E. Nicolas  Saman Seneweera 《Journal of Cereal Science》2014

In order to investigate the intra-specific variation of wheat grain quality response to elevated atmospheric CO₂ concentration (e[CO₂]), eight wheat (Triticum aestivum L.)cultivars were grown at two CO₂ concentrations ([CO₂]) (current atmospheric, 389 CO₂ μmol mol⁻¹vs. e[CO₂], FACE (Free-Air CO₂ Enrichment), 550  ±  10% CO₂ μmol mol⁻¹), at two water levels (rain-fed vs. irrigated) and at two times of sowing (TOS₁, vs. TOS₂). The TOS treatment was mainly imposed to understand whether e[CO₂] could modify the effects of timing of higher grain filling temperatures on grain quality. When plants were grown at TOS₁, TKW (thousand kernel weight), grain test weight, hardness index, P, Ca, Na and phytate were not significantly changed under e[CO₂]. On the other hand, e[CO₂] increased TKW (16%), hardness index (9%), kernel diameter (6%), test weight (2%) but decreased grain protein (10%) and grain phytate (11%) at TOS₂. In regard to grain Zn, Mn and Cu concentrations and some flour rheological properties, cultivar specific responses to e[CO₂] were observed at both sowing times. Observed genetic variability in response to e[CO₂] in terms of grain minerals and flour rheological properties could be easily incorporated into future wheat breeding programs to enable adaptation to climate change.  相似文献