乌苏里拟鲿(Pseudobagrus ussuriensis)同工酶分析     

潘伟志  尹洪滨  孙中武  葛学亮  柯冬蕾 《东北林业大学学报》2006,34(6):66-69

采用聚丙烯酰胺凝胶电泳方法,对黑龙江水系乌苏里拟鲿(Pseudobagrus ussuriensis)心、肝、肾、眼、肌肉和性腺共6种组织进行了9种同工酶(ADH、EST、G6PDH、GDH、IDH、LDH、MDH、POD、SOD)的分析。结果表明,乌苏里拟鲿EST、GDH、IDH、POD同工酶谱中存在不同程度的组织特异性,EST表现出明显的性别差异性。共记录19个基因位点,多态位点百分数P=21.05%,种群遗传偏离指数d=-1,该乌苏里拟鲿种群内部杂合子缺失较严重,偏离Hardy-Weinberg平衡,遗传多样性较低。  相似文献   

Antiplatelet and Antithrombotic Effects of Isaridin E Isolated from the Marine-Derived Fungus via Downregulating the PI3K/Akt Signaling Pathway     

Ni Pan  Zi-Cheng Li  Zhi-Hong Li  Sen-Hua Chen  Ming-Hua Jiang  Han-Yan Yang  Yao-Sheng Liu  Rui Hu  Yu-Wei Zeng  Le-Hui Dai  Lan Liu  Guan-Lei Wang 《Marine drugs》2022,20(1)

Isaridin E, a cyclodepsipeptide isolated from the marine-derived fungus Amphichorda felina (syn. Beauveria felina) SYSU-MS7908, has been demonstrated to possess anti-inflammatory and insecticidal activities. Here, we first found that isaridin E concentration-dependently inhibited ADP-induced platelet aggregation, activation, and secretion in vitro, but did not affect collagen- or thrombin-induced platelet aggregation. Furthermore, isaridin E dose-dependently reduced thrombosis formation in an FeCl₃-induced mouse carotid model without increasing the bleeding time. Mechanistically, isaridin E significantly decreased the ADP-mediated phosphorylation of PI3K and Akt. In conclusion, these results suggest that isaridin E exerts potent antithrombotic effects in vivo without increasing the risk of bleeding, which may be due to its important role in inhibiting ADP-induced platelet activation, secretion and aggregation via the PI3K/Akt pathways.  相似文献   

Identification of QTLs Associated with Resistance to Pseudomonas syringae pv.Glycinea in Soybean (Glycine max (L.) Merr)     

Mei Hong-yao  Liu Yang  Pan Xiao-cheng  Su An-yu  Wu Xiao-xia 《东北农业大学学报(英文版)》2021,28(2):1-14

Soybean bacterial spot disease caused by Pseudomonas syringae pv.Glycinea which is a bacterial disease seriously affects soybean yield.Ten soybean germplasms and recombinant inbred lines (RILs) population were used to identify the resistant trait after inoculated with P.sg (P.sgneau001) in this study.High-density genetic mapping was obtained by specific length amplified fragment sequencing (SLAF-seq) of 149 RILs population which was derived from the crossing between Charleston and Dongnong594.The results indicated that 10 germplasm resources had four resistant germplasms included highly resistant cultivar Charleston,four susceptible varieties included Dongnong594 and two moderately resistant cultivars.Five quantitative trait locus (QTLs) were detected in RILs population by the composite interval mapping (CIM) method,and located on Linkage Group (LG) D1b (chromosome two),LG C2 (chromosome six) and LG H (chromosome 12),respectively.LOD scores ranged from 2.68 to 4.95 and the phenotypic variation percentage was from 6% to 11%.Six candidate genes were detected,according to the result of gene annotation information.Four of them had relationship with protein kinase activity,protein phosphorylation and leucine rich repeat (LRR) transmembrane protein,which had high expression after inoculated with P.sg by qRT-PCR.  相似文献   

Identification of two glycerophosphodiester phosphodiesterase genes in maize leaf phosphorus remobilization     

Jingxin Wang  Wenbo Pan  Alexiy Nikiforov  William King  Wanting Hong  Weiwei Li  Yang Han  Jana Patton-Vogt  Jianbo Shen  Lingyun Cheng 《作物学报(英文版)》2021,(1):95-108

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施用生物有机肥对云当归产量、品质及土壤养分的影响          下载免费PDF全文

杜彩艳  蜂述先  杨鹏  孙曦  王攀磊  此主拉姆  李卫林  彭慧娉 《中国土壤与肥料》2024,(1):149-156

为给云南省维西县云当归高产、优质和合理施肥提供理论依据,采用防草布覆盖开展云当归成药栽培田间试验,设置不施肥(CK1)、常规施肥(CK2)、单施化肥(C)、单施生物有机肥Ⅰ号(B_Ⅰ)、单施生物有机肥Ⅱ号(B_Ⅱ)、50%化肥+生物有机肥Ⅰ号(1/2C+B_Ⅰ)、50%化肥+生物有机肥Ⅱ号(1/2C+B_Ⅱ)7个处理,研究了不同施肥处理对云当归产量构成因素、药材产量、品质和土壤养分的影响。结果表明,(1)施用生物有机肥能促进云当归主根长、根粗、侧根数、单株鲜重、干重增加,显著提高云当归鲜、干药材产量(P<0.05),生物有机肥处理云当归鲜、干药材产量较CK1分别增产41.50%～201.13%、35.00%～69.42%,其中,1/2C+BⅠ处理鲜干产量最高,1/2C+BⅡ处理次之。(2)施用生物有机肥可提高云当归药材阿魏酸、挥发油、浸出物和多糖含量,降低总灰分含量,有效改善云当归药材品质;(3)施用生物有机肥可显著提高土壤有机质、碱解氮、有效磷和速效钾的含量(P<0.05)。施用生物有机肥处理...  相似文献   

Basal internode elongation of rice as affected by light intensity and leaf area     

Xuhua Zhong  Kaiming Liang  Bilin Peng  Ka Tian  Xiaojuan Li  Nongrong Huang  Yanzhuo Liu  Junfeng Pan 《作物学报(英文版)》2020,(1):62-70

Short basal internodes are important for lodging resistance of rice(Oryza sativa L.).Several canopy indices affect the elongation of basal internodes,but uncertainty as to the key factors determining elongation of basal internodes persists.The objectives of this study were(1)to identify key factors affecting the elongation of basal internodes and(2)to establish a quantitative relationship between basal internode length and canopy indices.An inbred rice cultivar,Yinjingruanzhan,was grown in two split-plot field experiments with three N rates(0,75,and 150 kg N ha−1 in early season and 0,90,and 180 kg N ha−1 in late season)as main plots,three seedling densities(16.7,75.0,and 187.5 seedlings m−2)as subplots,and three replications in the 2015 early and late seasons in Guangzhou,China.Light intensity at base of canopy(Lb),light quality as determined from red/far-red light ratio(R/FR),light transmission ratio(LTR),leaf area index(LAI),leaf N concentration(NLV)and final length of second internode(counted from soil surface upward)(FIL)were recorded.Higher N rate and seedling density resulted in significantly longer FIL.FIL was negatively correlated with Lb,LTR,and R/FR(P<0.01)and positively correlated with LAI(P<0.01),but not correlated with NLV(P>0.05).Stepwise linear regression analysis showed that FIL was strongly associated with Lb and LAI(R2=0.82).Heavy N application to pot-grown rice at the beginning of first internode elongation did not change FIL.We conclude that FIL is determined mainly by Lb and LAI at jointing stage.NLV has no direct effect on the elongation of basal internodes.N application indirectly affects FIL by changing LAI and light conditions in the rice canopy.Reducing LAI and improving canopy light transmission at jointing stage can shorten the basal internodes and increase the lodging resistance of rice.  相似文献   

Application method affects pesticide efficiency and effectiveness in wheat fields     

Jinjing Xiao  Li Chen  Fan Pan  Yajing Deng  Chenchun Ding  Min Liao  Xianyan Su  Haiqun Cao 《Pest management science》2020,76(4):1256-1264

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Quizalofop‐p‐ethyl resistance in Polypogon fugax involves glutathione S‐transferases     

Wen Chen  Lamei Wu  Junzhi Wang  Qin Yu  Lianyang Bai  Lang Pan 《Pest management science》2020,76(11):3800-3805

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江西双季稻机插的典型模式及其效果研究     

吴青香  曾勇军  程慧煌  成臣  石庆华  潘晓华 《中国农学通报》2020,36(3):1-6

基于江西省双季稻区10个县30个乡镇农户、合作社以及农机大市场的调查数据,通过对合作社的两种典型模式（合作模式和服务模式）的研究及合作社农户和散户的对比分析,探索合作社对江西双季稻机插推广的促进作用。研究结果表明:合作社农户的成本要低于散户,收益要高于散户,合作社的生产效果比散户的要好;合作社农户的年龄比散户的年龄要低,文化、土地规模、资金要高于散户;合作社农户的机插比例高于散户;目前江西合作社机插典型模式中比较受欢迎的模式是农机大户和种田大户合作模式及“订单式”服务模式;未来种田大户、农机大户和养殖大户是发展江西农民合作生产的主要对象,重点发展的模式是合作社提供水稻生产全程社会化服务模式。  相似文献   

11份玉米自交系单株产量等性状的配合力与遗传参数研究     

胡军  魏开锋  郭鹏程  刘万  于汇琳  潘洪玉  张鑫生 《中国农学通报》2020,36(6):5-10

旨在研究玉米自交系单株产量等性状的配合力、遗传力及反交效应,为玉米自交系的选育和杂交种的组配提供依据。以11份玉米自交系为试材,按Griffing Ⅲ完全双列杂交法组配110个组合,观测杂交种的单株产量、株高、穗位高、雄穗分支数、雄穗主轴长、抽丝期和开花期等7个性状的表型数据,并对上述性状的一般配合力、特殊配合力、广义遗传力、狭义遗传力和反交效应进行估算。供试材料除雄穗主轴长的特殊配合力差异不显著外,其余性状的一般配合力和特殊配合力差异均达到极显著水平。JZ3和JZ6两个自交系单株产量的一般配合力为极显著正值,两对组合JZ9×JZ2和JZ2×JZ9、JZ6×JZ3和JZ3×JZ6的单株产量具有最大的正向SCA效应值,分别为40.68 g和35.24 g。单株产量的反交效应差异极显著,部分自交系的反交效应方差较大。7个性状的广义遗传力从大到小依次为,雄穗分支数、株高、开花期、穗位高、抽丝期、单株产量和雄穗主轴长;狭义遗传力从大到小依次为,雄穗分支数、株高、穗位高、开花期、雄穗主轴长、抽丝期和单株产量。试验结果表明单株产量性状的显性遗传方差占比最大,狭义遗传力最小,易受环境条件的影响,对该性状的选择适宜在晚代进行;单株产量性状具有显著的反交效应,故部分自交系需严格控制正反交方式。  相似文献