Serodiagnostic comparison of enzyme-linked immunosorbent assay and surface plasmon resonance for the detection of antibody to Porcine circovirus type 2          下载免费PDF全文

Ho-Seong Cho  Tae-Jung Kim  Jae-Il Lee    Nam-Yong Park 《Canadian journal of veterinary research》2006,70(4):263-268

This paper describes the cloning and expression of the capsid protein of Porcine circovirus type 2 (PCV2) in an Escherichia coli expression system that was used to produce a fusion protein for subsequent immunologic studies: enzyme-linked immunosorbent assay (ELISA) and surface plasmon resonance (SPR). Polymerase chain reaction was used to amplify the gene encoding the capsid protein from the DNA of PCV2. The protein was then cloned into a pRSET prokaryotic expression vector. Western blot analysis revealed that the recombinant protein gave strong signals on a polyvinylidene difluoride membrane when exposed to the serum from a pig infected with PCV2. The expressed protein was purified and used as an antigen for the ELISA and SPR study. A protein chip based on SPR was developed, and the diagnostic potential of SPR was compared with that of ELISA with the use of 70 serum samples obtained from 6 pig farms. There was a strong positive correlation between the ELISA and SPR titers (r = 0.877, P < 0.01). Therefore, this recombinant capsid protein can be used as an antigen for serologic studies, and the SPR, a label-free method, appears to be a valuable and reproducible tool in the serodiagnosis of a PCV2 infection.  相似文献   

Genetic characterization of canine rotavirus isolated from a puppy in Korea and experimental reproduction of disease.   总被引：3，自引：0，他引：3  

Bo Kyu Kang  Dae Sub Song  Kwon Il Jung  Chul Seung Lee  Sung Jun Park  Jin Sik Oh  Dong Jun An  Jeong Sun Yang  Hyoung Joon Moon  Sang Sun Lee  Young Dhuk Yoon  Bong Kyun Park 《Journal of veterinary diagnostic investigation》2007,19(1):78-83

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C-reactive protein, tumor necrosis factor α, and interleukin-6 in dogs with pyometra and SIRS     

Boel A. Fransson  DVM  PhD  DACVS    Anne-Sofie Lagerstedt  DVM  PhD    Annika Bergstrom  DVM    Ragnvi Hagman  DVM  MSc  PhD    Jean S. Park  PhD    Boon P. Chew  DVM  PhD    Marc A. Evans  Claude A. Ragle  DVM  DACVS  DABVP 《Journal of Veterinary Emergency and Critical Care》2007,17(4):373-381

Objective: To determine the frequency of the systemic inflammatory response syndrome (SIRS) in canine pyometra and to evaluate the relationship between C‐reactive protein (CRP), tumor necrosis factor α (TNFα), interleukin‐6 (IL‐6), and SIRS. Design: Prospective clinical study. Setting: Veterinary teaching hospital. Animals: Fifty‐three clinical cases of canine pyometra and 19 healthy control bitches. Interventions: Upon admission to the veterinary hospital, history and physical examination findings, including previously defined clinical SIRS parameters, were documented. Blood samples were obtained for hematology and biochemical tests and for CRP, TNFα, and IL‐6 analysis. The diagnosis of pyometra was confirmed by histopathology of the uterus after ovariohysterectomy. After surgery, clinical SIRS parameters, length of hospitalization, and mortality were recorded. Measurements and main results: Pyometra dogs were grouped as SIRS positive (30/53; 57%) or SIRS negative (23/53; 43%). Logistic regression showed that CRP was the only parameter that significantly related to SIRS apart from the clinical criteria that define this syndrome. The mortality rate was low (2/53; 3.8%), and conclusions regarding association with SIRS could not be drawn. A positive SIRS status, high plasma CRP concentration, and high body temperature were variables that related to increased morbidity reflected by the length of hospitalization. Conclusions: SIRS was seen in 57% of canine pyometra cases and a positive SIRS status showed a positive association with prolonged hospitalization. The mortality rate was low (3.3%) among SIRS positive dogs, indicating that progression to multiple organ dysfunction syndrome (MODS) rarely occurs in surgically treated cases of pyometra. CRP was associated with SIRS and with prolonged hospitalization. Further studies of plasma CRP may be warranted in canine intensive care cases susceptible to development of SIRS and MODS.  相似文献   

肉鹅H5亚型禽流感疫苗免疫效果的研究   总被引：2，自引：0，他引：2  

张济培  陈建红  陈响坤  卢玉葵  司兴奎  吴悦霞 《中国畜牧兽医》2006,33(12):94-97

选用H 5亚型禽流感油乳剂灭活疫苗(H 5N 1,R e1株)对马岗鹅作首免日龄、免疫次数与免疫剂量的免疫试验。结果表明,雏鹅在7、14或21日龄作1次免疫均产生免疫应答,其中14与21日龄免疫组免疫后第5周抗体水平均达4 log2以上,抗体动态变化均值与峰值均高于7日龄免疫组2~3个滴度。用0.5、1和2 m l/只3个剂量对14日龄雏鹅的免疫试验中,0.5m l组抗体水平上升过程缓慢,免疫后4周抗体水平才达4 log2以上,1与2 m l组在免疫1周后上升速度较快,免疫后2周即达4 log2以上,抗体水平相近,但2 m l免疫组免疫后5~7周抗体水平均低于1 m l免疫组。说明接种剂量为1或2 m lH 5N 1油苗比接种0.5 m l的免疫效果好,尤以1 m l免疫剂量效果最好。雏鹅在7、14日龄或14、21日龄作2次免疫和7、14、21日龄作3次免疫,首免后抗体上升速度快,各检测点抗体水平均值相近,均在首免后3周达到4 log2以上,4~5周达6 log2以上,4~7周的抗体水平均高于14日龄1次免疫组,且提前2周达到4 log2以上,说明2次、3次免疫组的抗体水平高于1次免疫组,而2次与3次免疫组的抗体水平无明显差异。以上结果说明,在肉鹅生产的禽流感免疫中,选择以14日龄首免0.5 m l/只,21日龄2免接种1 m l/只可取得较为理想的免疫效果。  相似文献   

杀菌剂啶菌噁唑的高效液相色谱分析     

韩平  刘鹏飞  司乃国  刘西莉 《农药学学报》2006,8(3):288-290

建立了一种用反相高效液相色谱测定啶菌噁唑的定量分析方法。采用C18ODS色谱柱,以甲醇-水(75∶25,体积比)作为流动相,流速1.0mL/m in,检测波长220nm。啶菌噁唑色谱图显示两个吸收峰,分别为两个同分异构体Z体和E体。在400700μg/mL浓度范围内,标准溶液浓度和吸收峰面积之间有良好的线性关系,回归方程为Y=4.846 7x+232.85,相关系数r为0.999 2;对原药溶液及其制剂溶液测定的重复性SR分别为0.004 0和0.004 7。该方法准确、精密,适用于啶菌噁唑的定量分析。  相似文献   

Effectiveness of 99mTc-tetrofosmin for assessment of heart functions in micropigs     

Min Young Lee  Sang Hun Lee  Jae Hong Park  Jung Sun Heo  Yu Jin Lee  Han Na Suh  Jung Jun Min  Young Soon Seo  Ho Jae Han 《Journal of veterinary science (Suw?n-si, Korea)》2007,8(3):223-227

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Canine model of ischemic stroke with permanent middle cerebral artery occlusion: clinical and histopathological findings     

Byeong-Teck Kang  Jong-Hwan Lee  Dong-In Jung  Chul Park  Su-Hyun Gu  Hyo-Won Jeon  Dong-Pyo Jang  Chae-Young Lim  Fu-Shi Quan  Young-Bo Kim  Zang-Hee Cho  Eung-Je Woo  Hee-Myung Park 《Journal of veterinary science (Suw?n-si, Korea)》2007,8(4):369-376

The aim of the present study was to assess the clinical and histopathological findings in a canine model of ischemic stroke. Cerebral ischemic stroke was induced by middle cerebral artery occlusion in four healthy beagle dogs using silicone plugs. They showed neurological signs of forebrain dysfunction such as reduced responsiveness, head turning, circling, postural reaction deficits, perceptual deficits, and hemianopsia. These signs gradually regressed within 4 weeks without therapy. On magnetic resonance imaging, T2 hyperintensity and T1 hypointensity were found in the cerebral cortex and basal ganglia. These lesions were well-defined and sharply demarcated from adjacent brain parenchyma with a homogenous appearance. No abnormalities of the cerebrospinal fluid were observed. At necropsy, atrophic and necrotic lesions were observed in the cerebral cortex. The cerebral cortex, basal ganglia, and thalamus were partially unstained with triphenyl-tetrazolium chloride. Histopathologically, typical features of infarction were identified in cortical and thalamic lesions. This study demonstrates that our canine model resembles the conditions of real stroke patients.  相似文献   

高效毛细管电泳同时测定4种氟喹诺酮类抗菌药     

王艳红  檀华蓉  祁克宗  司雄元 《中国兽药杂志》2007,41(11):8-11

建立同时测定4种氟喹诺酮类抗菌物(左氧氟沙星、培氟沙星、沙拉沙星和洛美沙星)的高效毛细管电泳(HPCE)法。考察了缓冲液离子浓度和pH、分离电压、运行温度等电泳参数,确立了最佳的电泳条件:缓冲液为10 mmol/L磷酸二氢钾-20 mmol/L硼砂(pH9.0,内含35mmol/L SDS),紫外检测波长278 nm,分离电压28 kV,运行温度25℃。结果表明,4种药物在8min内得到完全分离,分离度R为1.2以上。方法精密度(RSD)为0.22%~0.26%(迁移时间)和1.36%~2.39%(峰面积),回收率99.60%以上。该法快速、简便、准确。  相似文献   

微量元素硒对成年皖西白鹅输卵管膨大部粘蛋白的影响   总被引：1，自引：4，他引：1  

马兆臣  李福宝  葛凯  司梅霞  王彗 《中国畜牧兽医》2006,33(11):15-18

应用组织化学PA S-AB染色和定量图像分析法,研究了不同水平微量元素硒对成年皖西白鹅输卵管膨大部粘蛋白的影响,结果表明:日粮中添加0.2 m g/kg微量元素硒鹅输卵管膨大部粘蛋白与对照组(不加硒)差异显著(P<0.05);日粮中添加0.60 m g/kg与对照组差异极显著(P<0.01);当日粮中添加硒到1.1 m g/kg时,与对照组差异不显著(P>0.05);说明随着硒添加量的加大,皖西白鹅会发生慢性中毒。因此,微量元素硒对成年皖西白鹅输卵管膨大部粘蛋白的影响有显著差异。  相似文献   

4味中药及其与抗菌药的复方制剂的MIC测定   总被引：3，自引：0，他引：3  

司红彬  梁松林  许桂芹  王世坤  胡功政 《中国兽药杂志》2006,40(2):31-34

用改进的试管两倍稀释法测定了黄连、鱼腥草、大青叶和苦参4味中药及其相互配伍（1：1）对标准大肠杆菌的最小抑菌浓度和4味中药与抗菌药配伍（1000：1）对临床分离鸡大肠杆菌的最小抑菌浓度。结果表明，4味中药及其相互配伍对大肠杆菌标准株有一定的抑制作用，其MIC值介于3．91—62．50mg／mL之间，其中大青叶和鱼腥草以1：l配伍对大肠杆菌抑菌效果最好，MIC为3．91mg／mL；对临床分离株，单方药中黄连抑菌效果最好，MIC为31．25mg／mL，24种复方药中，黄连和盐酸多西环素或加替沙星以1000：1配伍的抑菌效果最好，其MIC为3．91mg／mL。  相似文献