Effect of anthocyanin fractions from selected cultivars of Georgia-grown blueberries on apoptosis and phase II enzymes     

Srivastava A  Akoh CC  Fischer J  Krewer G 《Journal of agricultural and food chemistry》2007,55(8):3180-3185

In recent years, considerable attention has been paid to anthocyanins due to their abilities to inhibit oxidative stress and cell proliferation. The regulations of apoptosis and the phase II enzymes glutathione-S-transferase (GST) and quinone reductase (QR) are other potential mechanisms through which flavonoids such as anthocyanins may prevent cancer. Our study confirmed that anthocyanin fractions from high bush blueberry cultivars increased apoptosis using two different methods: DNA fragmentation and caspase-3 activity. The effect of anthocyanins on the activity of the detoxifying enzymes GST and QR was also determined. Major anthocyanins identified were delphinidin, cyanidin, peonidin, petunidin, and malvidin. In Tifblue and Powderblue cultivars, DNA fragmentation increased at anthocyanin concentrations from 50 to 150 microg/mL, but cells treated with the anthocyanin fraction of Brightblue and Brightwell showed a prominent ladder at 50-100 microg/mL when compared to cells treated with 150 microg/mL. There was a significant difference in the caspase-3 activity (P < 0.05) between the control cells and the cells treated with anthocyanins from all of the cultivars. The response correlated positively with dose. The QR activity was lower in all cells treated with an anthocyanin fraction from Tifblue, Powderblue, Brightblue, and Brightwell cultivars than in control cells (P < 0.05). The activity decreased gradually when treated with increased concentrations of anthocyanin fractions (50-150 microg/mL) in the Tifblue and Powderblue cultivars. The GST activity was lower (P < 0.05) in cells treated with anthocyanin fractions from all of the cultivars and at all concentrations. These results indicated that apoptosis was confirmed in HT-29 cells when treated with anthocyanins from blueberry cultivars at 50-150 microg/mL concentrations, but these same concentrations decrease QR and GST activities rather than induce them.  相似文献   

Projecting global and regional outlooks for planted forests under the shared socio-economic pathways     

Korhonen  Jaana  Nepal  Prakash  Prestemon  Jeffrey P.  Cubbage  Frederick W. 《New Forests》2021,52(2):197-216

New Forests - There is rising global interest in growing more trees in order to meet growing population, climate change, and wood energy needs. Using recently published data on planted forests by...  相似文献   

Composite wound dressing for drug release     

Roli Purwar  Poonam Rajput  Chandra Mohan Srivastava 《Fibers and Polymers》2014,15(7):1422-1428

Present study is focused on the preparation of two layers composite wound dressing for drug release. The outer layer is made of hydrogel which contains of drug and the core layer is made of fabric. The two layers structure of composite dressing is formed by grafting of polyacrylamide-co-acrylic acid hydrogel on cotton fabric using ammonium per sulphate (APS) as chemical initiator and polyethylene glycol (PEG) as crosslinker. The major factors affecting graft copolymerization of hydrogel on cotton fabric are optimized by varying concentration of monomers & initiator, reaction temperature and addition time of crosslinker. Maximum grafting of hydrogel is obtained at 5 % (w/v) APS and 15 % acrylamide/acrylic acid (1:1 w/w ratio) concentration. The FTIR spectra of composite dressing shows characteristics peak of acrylic acid and acrylamide. The composite wound dressing material is loaded with model drug bovine serum albumin (BSA) and drug release behaviour is studied at different pH. The dressing shows drug release in different pH with maximum release of drug in acidic medium.  相似文献   

Development and characterization of microsatellite markers,genetic diversity and population structure analysis in Sapota (Manilkara zapota (L.) P. Royen)     

Sathanandam  Pavithra N.  Patil  Prakash  Rekha  Ajitha  Muthuvel  Iyamperumal  Patel  Amrutlal R.  Boggala  Ramesh B.  Shirol  Adiveppa M.  Ravishankar  Kundapura V. 《Genetic Resources and Crop Evolution》2022,69(8):2787-2801

Genetic Resources and Crop Evolution - Manilkara zapota (L.) P. Royen, a widely adapted and popular tree meant for its appetizing fruits in tropics with no genomic resources like microsatellite...  相似文献   

BOOK REVIEW: Soil Analysis. Edited by S. K. Singh,D. R. Biswas,C. A. Srinivasamurthy,S. P. Datta,G. Jayasree,P. Jha,S. K. Sharma,R. N. Katkar,K. P. Raverkar,A. K. Ghosh,Indian Society of Soil Science,New Delhi,India, 2019, pp. INR1400, ISBN 81-903797-8-X.     

Sabyasachi Majumdar  Laxmanarayanan Muruganantham  Kapudeep Karmakar  Prakash Nagabovanalli Basavarajappa 《European Journal of Soil Science》2022,73(4):e13259

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Mitochondrial phylogeography reveals high haplotype diversity and unique genetic lineage in Indian dugongs (Dugong dugon)     

Yellapu Srinivas  Anant Pande  Swapnali Gole  P.V.R. Prem Jothi  K. Madhu Magesh  Sameeha Pathan  Sohini Dudhat  Rukmini Shekar  Chinmaya Ghanekar  Devanshi Kukadia  Jeyaraj Antony Johnson  Samrat Mondol  Kuppusamy Sivakumar 《水产资源保护:海洋与淡水生态系统》2021,31(4):818-829

1. India plays a significant role in dugong conservation by having the largest population within South Asia. The status of dugongs in India is largely unknown due to a paucity of reliable ecological data. This study generated mitochondrial control region sequences from ~10% of dugong individuals from existing populations within India. Furthermore, data generated in this study were compared with the global data to assess genetic lineages, population structure, and genetic diversity of Indian populations.
2. Multiple analyses suggest that the Indian dugong populations are part of a single genetic cluster, comprising South Asia, North-west Indian Ocean, and South-west Indian Ocean populations. Despite small population size, they retain high genetic diversity with unique mitochondrial DNA haplotypes within South Asia. Within India, novel haplotypes are observed from all dugong habitats sampled, with overall high haplotype diversity (0.85 ± 0.04) but low nucleotide diversity (0.005 ± 0.001). Indian populations exhibit genetic differentiation with higher within-population variance (63.41%) than among populations (36.59%). Two of the haplotypes observed in India are shared with Sri Lanka, implying genetic connectivity between these populations.
3. The genetic data from Indian dugong populations provide critical insights into the identification of dugong corridors and important dugong conservation zones in India. We suggest site-specific interventions, including the creation of new marine protected areas and boundary reorganization and expansion of other existing protected areas, to ensure population connectivity. In addition, simultaneous efforts towards seagrass meadow restoration, reduction of dugong mortalities, and community participation in dugong conservation are recommended for population recovery of this threatened marine herbivore.

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Estimation of gut contents of freshwater mussel, Lamellidens marginalis L.     

Rathindra Nath Mandal  Kuldeep Kumar  Utkal Laxmi Mohanty  & Prem Kumar Meher 《Aquaculture Research》2007,38(13):1364-1369

This study examined the food organisms found in the gut of freshwater mussels, Lamellidens marginalis L. A total of 34 taxa of food organisms were recorded, out of which 30 taxa belonged to phytoplankton and four taxa to zooplankton. Both the groups comprised three families each: Cyanophyceae (blue‐green algae), Chlorophycea (green algae) and Bacillariophyceae (diatoms) constituted phytoplankton, whereas Euglenaceae, Chlamydodontidae and Brachionidae belonged to zooplankton. The total number of frequencies of phytoplankton (n=537) were almost nine times more than that of zooplankton (n=60). When blue‐green algae, green algae, diatoms and zooplankton (all the three families together) were tested for significant difference (P<0.05) following Duncan's multiple range test, the result showed only two groups. Blue‐green algae, green algae and zooplankton were not significantly different (P<0.05), forming group A, whereas diatoms were significantly different from others, forming group B. The present study showed that the maximum number of organisms that mussels feed upon belong to green algae, followed by diatoms, blue‐green algae and few taxa of zooplankton.  相似文献   

Comparative sequence analysis of 16S rRNA gene of Pasteurella multocida serogroup B isolates from different animal species     

Dey S  Singh VP  Kumar AA  Sharma B  Srivastava SK  Singh N 《Research in veterinary science》2007,83(1):1-4

The phylogenetic relationships of five isolates of Pasteurella multocida serotype B:2 belonging to buffalo, cattle, pig, sheep and goat were investigated by comparative sequence analysis of 16S rRNA gene. The 1468bp fragment of 16S rRNA gene sequence comparison showed that the isolates of cattle (PM75), pig (PM49) and sheep (PM82) shared 99.9% homology with the buffalo isolate (vaccine strain P52) whereas, the goat isolate (PM86) shared 99.8% homology with the vaccine strain. The 16S rRNA gene sequences of these isolates were also found monophyletic with type B reference strain NCTC 10323 of P. multocida subsp. multocida. The present study indicated the close relationships of haemorrhagic septicaemia causing P. multocida serotype B:2 isolates of buffalo and cattle with other uncommon hosts (pig, sheep and goat).  相似文献   

Cloning and Sequencing of a 21 kDa Outer Membrane Protein Gene of <Emphasis Type="Italic">Leptospira interrogans</Emphasis> serovar Canicola strain Hond Utrecht IV     

Cheema PS  Srivastava SK  Chaudhuri P  Amutha R  Singh S  Sankar M  Thangapandian E 《Veterinary research communications》2007,31(5):521-527

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Identification of RAPD markers linked to the rust (<Emphasis Type="Italic">Uromyces fabae</Emphasis>) resistance gene in pea (<Emphasis Type="Italic">Pisum sativum</Emphasis>)     

S.?Vijayalakshmi  Kusum?Yadav  Chanda?Kushwaha  S.?B.?Sarode  C.?P.?Srivastava  Ramesh?Chand  B.?D.?SinghEmail author 《Euphytica》2005,144(3):265-274

Summary Two RAPD markers linked to gene for resistance (assayed as pustule number cm⁻² leaf area) to rust [Uromyces fabae (Pers.) de Bary] in pea (Pisum sativum L.) were identified using a mapping population of 31 BC₁F₁ [HUVP 1 (HUVP 1 × FC 1] plants, FC 1 being the resistant parent. The analysis of genetics of rust resistance was based on the parents, F₁, F₂, BC₁F₁ and BC₁F₂ generations. Rust resistance in pea is of non-hypersensitive type; it appeared to be governed by a single partially dominant gene for which symbol Ruf is proposed. Further, this trait seems to be affected by some polygenes in addition to the proposed oligogene Ruf. A total of 614 decamer primers were used to survey the parental polymorphism with regard to DNA amplification by polymerase chain reaction. The primers that amplified polymorphic bands present in the resistant parent (FC 1) were used for bulked segregant analysis. Those markers that amplified consistently and differentially in the resistant and susceptible bulks were separately tested with the 31 BC₁F₁ individuals. Two RAPD makers, viz., SC10-82₃₆₀ (primer, GCCGTGAAGT), and SCRI-71₁₀₀₀ (primer, GTGGCGTAGT), flanking the rust resistance gene (Ruf) with a distance of 10.8 cM (0.097 rF and LOD of 5.05) and 24.5 cM (0.194 rF and a LOD of 2.72), respectively, were identified. These RAPD markers were not close enough to Ruf to allow a dependable maker-assisted selection for rust resistance. However, if the two makers flanking Ruf were used together, the effectiveness of MAS would be improved considerably.  相似文献