Multielemental speciation analysis of fungi porcini (Boletus edulis) mushroom by size exclusion liquid chromatography with sequential on-line UV-ICP-MS detection     

Wuilloud RG  Kannamkumarath SS  Caruso JA 《Journal of agricultural and food chemistry》2004,52(5):1315-1322

An analytical methodology to determine the molecular weight (MW) distribution patterns of several elements among different compounds present in commonly consumed edible mushrooms is presented in this work. A hyphenated technique based on size exclusion liquid chromatography (SEC) coupled on-line to UV and inductively coupled plasma mass spectrometry (ICP-MS) detection was used. The association of the elements to high and low MW fractions was confirmed with sequential detection by UV and ICP-MS. Separation of the fractions was performed by injecting a 100 microL sample volume to a Superdex 75 column. The effect of different mobile phases on the separation was evaluated. Additionally, three different extraction conditions including 0.05 mol L(-1) NaOH, 0.05 mol L(-1) HCl, and hot water at 60 degrees C were applied to extract the elemental species from the mushroom samples. Significant differences were observed in the chromatograms depending on the extraction conditions utilized. Optimization of the experimental variables involved in the SEC-UV-ICP-MS coupling was carried out. The method was applied to investigate the fractionation patterns of Bi, Co, Cu, Fe, I, Mo, Ni, Se, and Zn in fungi porcini (Boletus edulis) mushroom. The results obtained in this work indicate an important association of most of the elements to high MW fractions.  相似文献   

Anti-Mullerian hormone and its relationship to ovulation response and fertility in timed AI Bos indicus heifers     

Emiliana O. S. Batista  Lais M. Vieira  Bruno G. Freitas  Bruna M. Guerreiro  J. G. S. Carvalho  Rodolfo D. Mingoti  Guilherme Vasconcellos  Alexandre H. Souza  Jose Bento Sterman Ferraz  Pietro Sampaio Baruselli 《Reproduction in domestic animals》2020,55(6):753-758

This study evaluated the association between plasma anti-Mullerian hormone (AMH) concentration and fertility in Nelore (Bos indicus) heifers submitted to timed artificial insemination (TAI). At the onset of the synchronization protocol, heifers (n = 289) received a subcutaneous P4 ear implant (3 mg) and 2 mg of oestradiol benzoate. Eight days later, the P4 implant was removed and 0.5 mg of oestradiol cypionate, prostaglandin (0.265 mg, i.m.) and equine chorionic gonadotropin (300 UI, i.m.) was administered, and TAI was performed 48 hr after ear implant removal. Ovarian ultrasound evaluations were performed to measure number of ovarian follicles, dominant follicle size and ovulation response. Pregnancy diagnosis was performed by ultrasound 30 days after AI. Heifers with greater circulating AMH had more antral follicles, a smaller dominant follicle near timed ovulation and lower ovulation response to the timed AI protocol compared to heifers with lower circulating AMH. Although AMH and pregnancy outcome had a quadratic-shaped pattern, AMH was not significantly associated with fertility. In conclusion, heifers with lower AMH had larger follicles towards the end of the synchronization protocol and greater ovulation responses, whereas greater circulating AMH was unrelated to conception success.  相似文献   

Ontogeny of feeding apparatus and foregut of mud crab Scylla serrata Forsskål larvae     

Gil J Lumasag  Emilia T Quinitio  Riza O Aguilar  Rodolfo B Baldevarona  & Crispino A Saclauso 《Aquaculture Research》2007,38(14):1500-1511

The development of the feeding apparatus of the mud crab Scylla serrata larvae was studied using electron microscopy for mandibles and light microscopy for other paired mouthparts and the foregut. The six paired mouthparts, which consisted of the mandibles, maxillules, maxillae, first maxillipeds, second maxillipeds and third maxillipeds, were dissected from specimens representing each larval stage. The first five paired appendages were already present in newly hatched larvae while third maxillipeds appeared only at the megalopa stage. Mandibles displayed complex incisor and molar processes at early zoeal stages, which became simple in morphology at megalopa. Mandibular palp buds were observed at the zoea 5 stage and these became fully developed as three‐segmented mandibular palps at the megalopa stage. Endopods of other paired mouthparts exhibited increased number of setae and size as the individual metamorphosed from zoeal stages to megalopa and crab instar. The foregut appeared as a continuous cavity at zoea 1 where the cardiopyloric valve was indistinct while the filter gland was clearly identifiable. Zoea 2 and succeeding zoeal stages exhibited a setose foregut; the gastric mill and its lateral and median teeth were prominent at zoea 3 stage. The significance of these morphological changes is discussed in terms of its implication in larval feeding management.  相似文献   

The effect of dietary coconut oil on reproductive traits and egg fatty acid composition in rainbow trout (Oncorhynchus mykiss)     

Rodolfo Ballestrazzi  Simona Rainis  Francesca Tulli  Arianna Bracelli 《Aquaculture International》2003,11(3):289-299

Two hundred and twenty rainbow trouts (IBW: 700 g) were randomly allotted to four tanks, with a male/female ratio of 0.56. Fish were fed for 168 d with four experimental diets containing herring oil, cod liver oil and coconut oil with the following inclusion rates: diet A: 12-1-0% respectively; diet B: 6-1-6%; diet C: 0-1-12%; diet D: 0-0-13%. Irrespective of the dietary treatment, weight gains of broodstocks were high (> 3 g/d) and FCR below 2. No significant difference was observed concerning the total amount of eggs spawn, egg average weight (82.5 mg/egg) and lipid content (5.4 mg/egg). However, the fatty acid profile of eggs was significantly affected by the dietary treatments. The content of unsaturated fatty acids, particularly the n-3 fatty acid series (EPA and DHA) significantly decreased with increasing levels of coconut oil in the diet.  相似文献   

Low genetic variation and isolation of northern peripheral populations of a red seaweed (Grateloupia lanceola)     

Isabel Maneiro  Lucía Couceiro  Ignacio Bárbara  Javier Cremades  José M. Ruiz  Rodolfo Barreiro 《水产资源保护:海洋与淡水生态系统》2011,21(6):590-600

Effect of moisture on physical parameters of timber from Turkey oak (<Emphasis Type="Italic">Quercus cerris</Emphasis> L.) coppice in Central Italy     

Angela LO MONACO  Luigi TODARO  Mara SARLATTO  Raffaello SPINA  Luca CALIENNO  Rodolfo PICCHIO 《中国林学(英文版)》2011,13(4):276-284

This study aims to investigate wood density at different levels of moisture, basic density and shrinkage of timber from Turkey oak (Quercus cerris L.) coppice forests growing in Central Italy. We also studied the variability in density in the trees within and among sites. Density shows no significant statistical differences in the tested population. A higher variability in the shrinkage than in the density was found. Wood moisture is referred to as dry mass and fresh mass, which is related to many performance characteristics of wood, i.e., energy production. Trends in moisture and water content were studied because these physical parameters play an important role in the specific area of firewood which requires an accurate estimation of mass, volume and energy content. This work is a contribution to improve xylo-energy estimates of small and medium forestry issues.  相似文献   

Cytokine and transcription factor profiles in the skin of dogs naturally infected by Leishmania (Leishmania) chagasi presenting distinct cutaneous parasite density and clinical status     

Menezes-Souza D  Corrêa-Oliveira R  Guerra-Sá R  Giunchetti RC  Teixeira-Carvalho A  Martins-Filho OA  Oliveira GC  Reis AB 《Veterinary parasitology》2011,177(1-2):39-49

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Secretory patterns of growth hormone in dogs: circannual, circadian, and ultradian rhythms          下载免费PDF全文

Cristina Gobello  Yanina A. Corrada  Gervasio L. Castex  Rodolfo L. de la Sota    Rodolfo G. Goya 《Canadian journal of veterinary research》2002,66(2):108-111

The objective was to characterize the circannual, circadian, and ultradian secretory patterns of growth hormone (GH) in intact crossbred and purebred dogs. In all experiments, blood samples were collected with minimal stress by direct peripheral venipuncture and GH was measured in plasma by a homologous radioimmunoassay. For circannual studies, samples were collected monthly from 6 male dogs between 15:00 and 17:30 h over a 1-year time span. For circadian studies, blood samples were collected at 145-minute intervals from 09:00 to 06:45 h of the following day in 14 female dogs. In ultradian experiments, blood samples were collected at 15-minute intervals for 2.5 h (15:00 to 17:30 h) in 7 males and 7 females. Plasma GH in male dogs remained without change in summer, autumn, and winter but declined (P ≤ 0.01) in spring (LSM ± SEM; 6.9 ± 0.5; 6.0 ± 0.5; 6.3 ± 0.5; 4.3 ± 0.5 ng/mL, respectively). No plasma GH circadian rhythmicity was detected. Nor was any ultradian pattern evident in either males or females. No gender-related differences were observed in ultradian GH plasma profiles. It is concluded that, while basal GH levels show seasonal fluctuations in dogs, neither circadian nor ultradian GH secretory fluctuations were present in the dogs assessed.  相似文献   

Bio-efficacy of the Essential Oil of Oregano (<Emphasis Type="Italic">Origanum vulgare</Emphasis> Lamiaceae. Ssp. <Emphasis Type="Italic">Hirtum</Emphasis>)     

Ezequiel?Grondona  Gerardo?Gatti  Abel?G.?López  Leonardo?Rodolfo?Sánchez  Virginia?Rivero  Oscar?Pessah  María?P.?Zunino  Andrés?A.?PonceEmail author 《Plant foods for human nutrition (Dordrecht, Netherlands)》2014,69(4):351-357

The aim of this study was to investigate the bioactivity of the essential oil isolated from Origanum vulgare L. (EOv). We analyzed the in vivo anti-inflammatory properties in a mouse-airway inflammation model and the in vitro antimicrobial activity, genotoxicity over the anaphase-telophase with the Allium cepa strain and its cytotoxicity/viability in A549 culture cells. In vivo, EOv modified the levels of tumor necrosis factor -α and viable activated macrophages and was capable to mitigate the effects of degradation of conjugated dienes. In vitro, EOv reduced the viability of cultured A549 cells as well as the mitotic index and a number of chromosomal aberrations; however, it did not change the number of phases. We found that EOv presents antimicrobial activity against different Gram (?) and (+) strains, measured by disc-diffusion test and confirmed with a more accurate method, the AutoCad software. We postulate that EOv presents antibacterial, antioxidant and chemopreventive properties and could be play an important role as bioprotector agent.  相似文献   

LEAF-MACRONUTRIENT STATUS AND FRUIT YIELD OF BIOFERTILIZED YELLOW PASSION FRUIT PLANTS     

Lourival Ferreira Cavalcante  Ítalo Herbert Lucena Cavalcante  Francisco Rodolfo Júnior  Márkilla Zunete Beckmann-Cavalcante  Gaudêncio Pereira dos Santos 《Journal of plant nutrition》2013,36(2):176-191

Biofertilizers instead of synthetic chemicals are known to improve plant growth through the supply of plant nutrients and they may help sustaining environmental health and soil productivity. An experiment was carried out to evaluate fruit yield and macronutrient foliar contents of yellow passion fruit plants (Passiflora edulis) as a function of biofertilizers and nitrogen-phosphorus-potassium (N-P-K) fertilizing in Brazil (2005–2007). The experimental design was randomized in blocks with treatments, which were distributed in a factorial arrangement (3 × 2) referring to biofertilizer application [without biofertilizer, simple biofertilizer and enriched biofertilizer] and mineral fertilizing with NPK (fertilized and non-fertilized), with three replications of nine plants. Nutritional status of yellow passion fruit is affected by biofertilizer. Simple biofertilizer promotes optimum supplies of phosphorus, calcium and sulfur, while enriched biofertilizer promotes optimum supplies of phosphorus, potassium, calcium and sulfur. Both biofertilizers stimulated fruit yield, with results above Brazilian average.  相似文献