Protective efficacy of an H5/H7 trivalent inactivated vaccine produced from Re-11, Re-12, and H7-Re2 strains against challenge with different H5 and H7 viruses in chickens          下载免费PDF全文

ZENG Xian-ying  CHEN Xiao-han  MA Shu-jie  WU Jiao-jiao  BAO Hong-mei  PAN Shu-xin  LIU Yan-jing  DENG Guo-hua  SHI Jian-zhong  CHEN Pu-cheng  JIANG Yong-ping  LI Yan-bing  HU Jing-lei  LU Tong  MAO Sheng-gang  GUO Xing-fu  LIU Jing-li  TIAN Guo-bin  CHEN Hua-lan 《农业科学学报》2020,19(9):2294-2300

We developed an H5/H7 trivalent inactivated vaccine by using Re-11, Re-12, and H7-Re2 vaccine seed viruses, which were generated by reverse genetics and derived their HA genes from A/duck/Guizhou/S4184/2017(H5 N6)(DK/GZ/S4184/17)(a clade 2.3.4.4 d virus), A/chicken/Liaoning/SD007/2017(H5 N1)(CK/LN/SD007/17)(a clade 2.3.2.1 d virus), and A/chicken/Guangxi/SD098/2017(H7 N9)(CK/GX/SD098/17), respectively. The protective efficacy of this novel vaccine and that of the recently used H5/H7 bivalent inactivated vaccine against different H5 and H7 N9 viruses was evaluated in chickens. We found that the H5/H7 bivalent vaccine provided solid protection against the H7 N9 virus CK/GX/SD098/17, but only 50–60% protection against different H5 viruses. In contrast, the novel H5/H7 trivalent vaccine provided complete protection against the H5 and H7 viruses tested. Our study underscores the importance of timely updating of vaccines for avian influenza control.  相似文献   

灌溉方式与基施磷素对设施黄瓜产量的影响     

王月月  张晓晟  张强  陈宏坤  陈清 《北方园艺》2018,(9):1-7

以黄瓜为试材,采用小区试验对比的方法,研究了滴灌和畦灌条件下不同磷素用量对秋季大棚黄瓜产量的影响,以期为提高秋季大棚黄瓜产量提供参考依据。结果表明:滴灌大棚的黄瓜生长状况明显好于畦灌大棚,商品黄瓜产量显著提高(P0.05),各阶段用水总量278.3 mm,节水达41%。畦灌条件下,不施磷肥处理黄瓜果实产量显著低于磷肥处理(P0.05);滴灌条件下,过量的磷肥施用(P2O5100 kg·hm~(-2))导致黄瓜果实产量和品质显著下降(P0.05)。由此可见,滴灌条件下合理施用磷肥能够降低水资源消耗,提高磷肥利用效率,有利于黄瓜品质和产量的提升。  相似文献   

Do cover crops change the lability of phosphorus in a clayey subtropical soil under different phosphate fertilizers?          下载免费PDF全文

A. P. B. Teles  M. Rodrigues  W. F. Bejarano Herrera  A. Soltangheisi  L. R. Sartor  P. J. A. Withers  P. S. Pavinato 《Soil Use and Management》2017,33(1):34-44

Plants have developed different mechanisms to absorb and solubilize phosphorus (P) in the soil, especially in environments with low P availability. This study evaluated the effects of different winter cover crops on soil P availability in a clayey subtropical (Hapludox) soil receiving soluble P fertilizer and a rock phosphate applied to the summer crop, under no‐tillage. The experiment was carried out over 3 yrs (2009–2011) with five different cover crop species: common vetch, fodder radish, ryegrass, black oat, white clover and fallow as control. The soil was sampled after the third year of cover crop cultivation and analysed for inorganic and organic P forms according to the well‐established Hedley fractionation procedure. Phosphate fertilizers promoted accumulation of both labile and nonlabile P pools in soil in the near surface layer, especially under rock phosphate. Fertilizer applications were not able to change P fractions in deeper layers, emphasizing that the Brazilian clayey soils are a sink of P from fertilizer and its mobility is almost nil. Although the cover crops recycled a great amount of P in tissue, in a short‐term evaluation (3 yrs) they only changed the content of moderately labile P in soil, indicating that long‐term studies are needed for more conclusive results.  相似文献   

Pigeonpea improvement: An amalgam of breeding and genomic research     

Sameer Kumar Chanda Venkata  Ganga Rao Nadigatla Veera Prabha Rama  Rachit K. Saxena  Kulbhushan Saxena  Hari D. Upadhyaya  Moses Siambi  Said N. Silim  Kothapally Narasimha Reddy  Anupama J. Hingane  Mamta Sharma  Shivali Sharma  Stephen Dominic Lyimo  Rose Ubwe  Meshack Makenge  Kananji Gad  Paul Kiprotich Kimurto  Manuel Amane  Kennedy Kanenga  Yuventino Obong  Emanuel Monyo  Chris Ojiewo  Nagesh Kumar Mallela Venkata  Jaganmohan Polineni Rao  Prashanthi Lakkireddy  Sudhakar Chourat  Indraprakash Singh  Sobhan Sajja  Shruthi Hirikara Beliappa  Rajeev K. Varshney 《Plant Breeding》2019,138(4):445-454

In the past five decades, constant research has been directed towards yield improvement in pigeonpea resulting in the deployment of several commercially acceptable cultivars in India. Though, the genesis of hybrid technology, the biggest breakthrough, enigma of stagnant productivity still remains unsolved. To sort this productivity disparity, genomic research along with conventional breeding was successfully initiated at ICRISAT. It endowed ample genomic resource providing insight in the pigeonpea genome combating production constraints in a precise and speedy manner. The availability of the draft genome sequence with a large‐scale marker resource, oriented the research towards trait mapping for flowering time, determinacy, fertility restoration, yield attributing traits and photo‐insensitivity. Defined core and mini‐core collection, still eased the pigeonpea breeding being accessible for existing genetic diversity and developing stress resistance. Modern genomic tools like next‐generation sequencing, genome‐wide selection helping in the appraisal of selection efficiency is leading towards next‐generation breeding, an awaited milestone in pigeonpea genetic enhancement. This paper emphasizes the ongoing genetic improvement in pigeonpea with an amalgam of conventional breeding as well as genomic research.  相似文献   

The effect of feeding a novel multistrain yeast fraction on European seabass (Dicentrachus labrax) intestinal health and growth performance     

Mark D. Rawling  Nicola Pontefract  Ana Rodiles  Ilektra Anagnostara  Eric Leclercq  Marion Schiavone  Mathieu Castex  Daniel L. Merrifield 《Journal of the World Aquaculture Society》2019,50(6):1108-1122

Fish were fed a single‐strain yeast fraction (SsYF; 2 g/kg) or a multistrain yeast fraction (MsYF; 0.8 g/kg) for 10 weeks. The results demonstrated significant (p ≤ 0.03) elevations in weight gain, specific growth rate, protein efficiency ratio, and feed conversion ratio in fish fed the yeast fraction‐supplemented diets. In the distal intestine, a significant elevation in microvilli density was observed after 5 and 10 weeks of dietary supplementation with MsYF and SsYF, respectively, compared to control fed fish (p < 0.001). A significant elevation (p = 0.02) in the perimeter ratio was observed in fish fed diets supplemented with the yeast fractions. After 10 weeks of feeding on the experimental diets, Rt‐qPCR demonstrated a significant downregulation (p < 0.05) in the stress response genes, heat‐shock protein 70 (hsp70) and proliferating cell nuclear antigen (pcna), in fish fed diets supplemented with the yeast fractions. Significant (p < 0.05) elevations in interleukin 1‐beta (il1β) and interleukin‐10 (il10) gene expression were observed in fish fed diets supplemented with the MsYF compared to the other dietary groups. These findings suggest that feeding an MsYF specifically at a lower incorporation rate < 1 g/kg, compared to a commercial SsYF at 2 g/kg, is effective in improving the intestinal health status and growth performance of European seabass.  相似文献   

Changes in wettability of wood surface using electron beam irradiation     

H. Huber  R. Haas  A. Petutschnigg  G. Grüll 《Wood material science & engineering》2020,15(4):237-240

ABSTRACT

This study deals with the influence of electron beam irradiation (EBI) on the wettability of Norway spruce surface samples. To evaluate this possible effect in detail, the changes in chemistry and wood structure were analysed using the methods of IR spectroscopy and Scanning Electron Microscopy (SEM). The surface wettability was determined by the contact angle measurement method. The results of infrared spectra show decreased relative numbers of available hydrophilic groups (e.g. hydroxyl and carbonyl groups) in the wood surfaces due to the electron beam irradiation. This behaviour is consistent with the values from the surface wettability measurements. Structural differences of the wood surfaces after electron beam irradiation were not detectable. Based on these findings pre-treatment of wood surfaces for industrial usages can be a potential field of application of this technology.  相似文献   

特早熟高糖猕猴桃新品种‘金美’     

韩飞  李大卫  刘小莉  张鹏  陈美艳  田华  钟彩虹 《园艺学报》2020,47(Z2):2909-2910

‘金美’是从云南野外美味猕猴桃资源中选育出的绿肉高糖新品种。果实为卵形，果面黄褐色硬毛，果形整齐；平均单果质量60 ~ 80 g。果肉绿色或黄绿色，质嫩多汁，风味浓甜。软熟果实含可溶性固形物24.1%，可溶性总糖16.2%，总酸1.25%，维生素C 1 180 mg • kg-1，钾 342 mg • kg-1，钙84.2 mg • kg-1。在武汉地区8月果实成熟，此时果实采后7 d开始软熟，1 ~ 2 ℃低温和95%相对湿度下可存放100 ~ 120 d。盛果期产量22 t • hm-2。  相似文献   

香蕉新品种‘南天红’     

杨兴玉  肖维强  许林兵  李华平  黄秉智  叶春海  陈彪  陈谷  吕庆芳  梁钾贤  吴元立  胡玲玉 《园艺学报》2020,47(Z2):2961-2962

香蕉新品种‘南天红’由‘南天黄’的自然突变选育而成。假茎平均高度2.59 m，幼树假茎外表面暗红色，抽蕾后转绿。雄花苞片外表面常为深红色，花苞卷曲，常不脱落；从种植到收获11 ~ 13个月，单株产量25 kg，品质好。中高抗香蕉枯萎病4号小种，采用配套的栽培技术可在所有香蕉种植区推广。  相似文献   

光伏与农业结合的相关研究综述     

王玲俊  陈健 《安徽农业科学》2021,49(18):18-21,29

搜集了光伏与农业结合的相关文献,将其梳理为3个方面:光伏在农业中的应用、农光互补和光伏农业,进而对这3个方面的研究进行了综述.研究发现,光伏在农业中的应用主要用于解决农业生产中的能源短缺以及绿色生产等问题;农光互补则不同,它更加注重光伏发电与农业生产的相互影响、竞合关系以及耦合共生;而光伏农业是我国特有的提法,其含义包括光伏在农业中的应用和农光互补2个方面.未来的研究可从产业共生视角进行,分析光伏产业与农业共生融合的经济、社会和环境效应.  相似文献   

不同氮素水平下双季稻株型与冠层内光截获特征研究   总被引：1，自引：0，他引：1  

李艳大  黄俊宝  叶春  舒时富  孙滨峰  陈立才  王康军  曹中盛 《作物学报》2019,45(9):1375-1385

本文旨在阐明双季稻株型与冠层内光合有效辐射截获的时空分布特征。选用4个不同株型早、晚稻品种,设置4个不同施氮水平,系统观测其植株形态和冠层内光合有效辐射截获率(IPAR)的时空分布状况。结果表明,施氮水平对早、晚稻株高、穗长、叶长和叶基角均有显著影响,均表现为随施氮水平的增加而增大;早、晚稻孕穗期的分层叶面积指数(LAI)和向上累积LAI大于抽穗后12 d,分层LAI呈冠层中部大于上部和下部的分布特征,最大分层LAI出现在0.58相对高度处;冠层上中部分层LAI和向上累积LAI随施氮水平的增加而增大;向上累积LAI随相对高度呈S型曲线分布,可用Logistic方程定量描述(R~2 0.99);早、晚稻孕穗期的冠层IPAR大于抽穗后12 d,且随施氮水平的增加而增大,其日变化表现为正午较小,早晚较大;株型紧凑的早、晚稻品种,冠层IPAR低;冠层IPAR与向下累积LAI之间的关系可用方程IPAR=a (1-e~(-b×LAI))定量描述(R~2 0.88);冠层内IPAR的三维空间分布表现为冠层上中部水平面上IPAR较低,光斑变化大,冠层下部水平面上IPAR较高,光斑变化较平缓,同一冠层高度水平面上的IPAR呈不均匀分布。研究结果可为双季稻高产栽培及理想株型的优化设计提供支撑。  相似文献