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Shah S Yarrow C Dunning R Cheek B Vass S Windass J Hadfield S 《Pest management science》2012,68(1):38-48
BACKGROUND: Insecticide discovery screens carried out on whole organisms screen for potency resulting from chemical activity at the target site. However, many potentially insecticidal compounds are naturally detoxified in vivo and do not make it to the target site. It is hypothesised that insect strains with their xenobiotic detoxification machinery compromised could be used to identify such compounds that normally fail to show up in screens; these compounds could then be more rationally designed to increase their bioavailability. This strategy was tested with transgenic Drosophila lines with altered expression of Cyp6g1 and Dhr96. RESULTS: It was observed that Cyp6g1 knockdown transgenic lines have increased susceptibility to the test compound imidacloprid, while Dhr96 knockdown transgenic lines are resistant. Evidence was found for a systemic response to xenobiotic exposure, uncovered by piperonyl butoxide treatment and by gene expression profiling. Sex-specific gene expression regulated by DHR96 was also observed. CONCLUSION: The results confirm that this approach to chemical discovery could identify compounds that escape traditional screens. The complexity of the system means that a panel of single and multiple gene knockdown transgenic lines may be required. Copyright © 2011 Society of Chemical Industry 相似文献
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CHEN Ren-tian TANG Mao-yan WANG Qiang CHEN Lei LI Wei CHEN Chang Farooq Shah LIANG Tian-feng 《广西农业科学》2012,43(6)
全球气候变暖产生的局部短期极端高温,会对水稻形成高温胁迫,影响水稻的各种生理代谢过程,其出现频率有增加趋势.高温胁迫对水稻的影响主要是在开花期,极易导致严重的颖花不育.水稻耐高温胁迫能力存在基因型间的差异.在当天温度较低的时段开花,花粉活性高,花药长,花药基部开裂长度较长、开裂孔较大,是水稻耐高温胁迫的重要表型指标.热激蛋白的表达,提高花药的抗氧化能力,以及维持结构蛋白、酶和细胞膜的稳定性是增强柱头及柱头上花粉粒耐高温胁迫的重要生理机制.目前,国内外学者对高温胁迫影响水稻颖花受精结实的特征、水稻耐高温胁迫的形态与生理机理方面进行了大量研究,但未深入研究缓解水稻高温胁迫伤害的方法与策略,今后应结合水稻耐高温胁迫的形态与生理机理,加强有效缓解高温胁迫方法的探索与研究. 相似文献
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Genetic behaviour of fibre quality parameters under heat‐stressed conditions clearly reflected the significant effect of heat stress on the phenotypic expression of fibre quality parameters. Results from the field experiments demonstrated that fibre quality was better among the upland cotton cultivars under non‐stressed (June) regime. Fibre length, strength, uniformity and fineness were substantially high under June regime when compared with that under April (heat stressed) regime. The prevalence of significant genotype × temperature regime interaction for fibre length, fineness and strength provided another evidence for the influence of temperature regimes on the expression of fibre traits. A significant effect of heat stress on the phenotypic expression of fibre quality parameters was observed. 相似文献
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The purpose of this study was to investigate the potential benefits of yeast cell wall (YCW) on the gastrointestinal development of weaned calves. Twenty healthy Holstein male calves (BW = 92 ± 8.29 kg and 60 ± 5 d of age) were randomly allocated into 2 groups: CON with no YCW, and YCW (accounted for 0.16% of the basal diet). The dietary concentrate-to-roughage ratio was 40:60. All the calves were fed regularly twice a day at 09:00 and 16:00 and had free access to water. The experiment lasted for 60 d. The results showed that calves fed YCW showed higher (P < 0.05) length, width, and surface area of papillae in the ventral sac of the rumen as compared to CON. For the dorsal sac of the rumen, the muscularis thickness was thicker (P < 0.05) in the YCW group when compared with CON group. The villus height of YCW calves was higher (P < 0.05) than that of CON in the ileum. Calves supplemented with YCW also showed a higher (P < 0.05) villus height-to-crypt depth ratio in the ileum. The YCW calves exhibited a greater (P < 0.05) thickness of the wall in the duodenum and jejunum. Calves supplemented with YCW improved (P < 0.05) the claudin 1 mRNA expression in the ileum and occludin mRNA expression in the jejunum and ileum. The YCW increased (P < 0.05) the contents of secretory immunoglobulin A in the jejunum and ileum of calves. In conclusion, dietary supplementation with YCW could improve the gastrointestinal development of weaned calves. 相似文献
109.
Dalley JW Fryer TD Brichard L Robinson ES Theobald DE Lääne K Peña Y Murphy ER Shah Y Probst K Abakumova I Aigbirhio FI Richards HK Hong Y Baron JC Everitt BJ Robbins TW 《Science (New York, N.Y.)》2007,315(5816):1267-1270
Stimulant addiction is often linked to excessive risk taking, sensation seeking, and impulsivity, but in ways that are poorly understood. We report here that a form of impulsivity in rats predicts high rates of intravenous cocaine self-administration and is associated with changes in dopamine (DA) function before drug exposure. Using positron emission tomography, we demonstrated that D2/3 receptor availability is significantly reduced in the nucleus accumbens of impulsive rats that were never exposed to cocaine and that such effects are independent of DA release. These data demonstrate that trait impulsivity predicts cocaine reinforcement and that D2 receptor dysfunction in abstinent cocaine addicts may, in part, be determined by premorbid influences. 相似文献
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