Molecular divergence and application of the ITS-5.8S rDNA and RUBISCO spacer in <Emphasis Type="Italic">Porphyra haitanensis</Emphasis> Chang et Zheng (Bangiales,Rhodophyta)     

Changsheng Chen  Chaotian Xie  Dehua Ji  Yan Liang  Lingmin Zhao 《Aquaculture International》2010,18(6):1045-1060

To select a reliable and sensitive method for discriminating strains of Porphyra haitanensis, the nucleotide sequence of the internal transcribed spacer 1 to internal transcribed spacer 2 regions (ITS-5.8S) of nuclear ribosomal DNA and the intergenic spacer region of RUBISCO were compared in five wild and five cultivated Porphyra haitanensis strains. Based on molecular analyses, sequences of ITS-5.8S (about 1,210 bp) could be divided into three regions: ITS1, 5.8S, and ITS2. The ITS1 and ITS2 sequences of each strain differed, even between individuals collected from the same site. In contrast, 5.8S rDNA and RUBISCO spacer sequences were identical among the ten P. haitanensis strains, although differences were found among different Porphyra species. Phylogenetic analysis also supported these conclusions. These sequence features of highly conserved regions and diversified regions that occurred repeatedly in ITS-5.8S could be useful in discriminating germplasm of P. haitanensis strains or Porphyra species. In contrast, the RUBISCO spacer is only suitable for identifying Porphyra species. New coupled primers were designed to amplify only the 5.8S rDNA and ITS2 region of Porphyra. The sequences of these amplified fragments can be readily used to identify germplasm or to perform phylogenetic analysis of Porphyra spp.  相似文献   

机械运输对伊朗北部山林陡峭集材道土壤的干扰     

Ramin Naghdi  Iraj Bagheri  Reza Basiri 《林业研究》2010,21(4):497-502

A study was conducted to investigate the effects of skid trail slope and traffic levels on soil disturbances at two soil depths (0–10 and 10–20 cm). The treatments were set at four traffic levels (2, 7, 12 and 20), two slope classes (<20% and >20%) and two soil depths (0–10 and 10–20 cm). Results show that skidder traffic, longitudinal slope and soil depth have significant effect on soil bulk density in skid trail. Comparison of average soil bulk density in different traffic levels shows that there are significant differences in average bulk density between different traffic levels and control (p<0.05). The average bulk densities in different slopes and soil depths are significantly increased with increase in traffic levels, maximized at 12 passes (p<0.05), but there are no significant differences between 12 and 20 passes. The interaction effects between traffic and soil depth are significant (F _0.05,3=0.109, p<0.001). For all traffic treatments, there are significant differences in soil moisture content between the two slope classes and the two depths (p<0.001). However, the interaction effects between traffic levels and slope classes are not significant (p >0.05), although skidder traffic and slope affected soil moisture content.  相似文献   

Influence of Humic Products on Soil Health and Potato Production     

Mir-M Seyedbagheri 《Potato Research》2010,53(4):341-349

Since 1900, soil organic matter (SOM) in farmlands worldwide has declined drastically as a result of carbon turnover and cropping systems. Over the past 17 years, research trials were established to evaluate the efficacy of different commercial humates products on potato production. Data from humic acid (HA) trials showed that different cropping systems responded differently to different products in relation to yield and quality. Important qualifying factors included: source; concentration; processing; chelating or complexing capacity of the humic acid products; functional groups (Carboxyl; Phenol; Hydroxyl; Ketone; Ester; Ether; Amine), rotation and soil quality factors; consistency of the product in enhancing yield and quality of potato crops; mineralization effect; and influence on fertilizer use efficiency. Properties of humic substances, major constituents of soil organic matter, include chelation, mineralization, buffer effect, clay mineral-organic interaction, and cation exchange. Humates increase phosphorus availability by complexing ions into stable compounds, allowing the phosphorus ion to remain exchangeable for plants’ uptake. Collectively, the consistent use of good quality products in our replicated research plots in different years resulted in a yield increase from 11.4% to the maximum of 22.3%. Over the past decade, there has been a major increase in the quality of research and development of organic and humic acid products by some well-established manufacturers. Our experimentations with these commercial products showed an increase in the yield and quality of crops.  相似文献   

Studies on hollownesss regulation and properties of crosslinked hollow phenolic fibers     

Dongqing Zhang  Jingli Shi  Quangui Guo  Yan Song  Lang Liu 《Fibers and Polymers》2012,13(4):495-500

The hollowness of crosslinked hollow phenolic fibers was regulated successfully from 9 % to 80 % by adjusting the curing temperature of the partially crosslinked fibers. The partially crosslinked fibers was studied in detail by mass gained, tensile strength, solvent dissolution, SEM, IR, and TG analysis, and the prepared hollow phenolic fibers with different degrees of hollowness were characterized with SEM, tensile strength, TG-DSC and TG-MS. The results show that the factor determining the hollowness is the crosslinked extent of the partially crosslinked fibers and the hollow fibers with different degrees of hollowness have similar crosslinkage, mechanical properties and thermal stability.  相似文献   

Efficiency of methods applied for goat estrous synchronization in subtropical monsoonal climate zone of Southwest China     

Yongju Zhao  Jiahua Zhang  Hong Wei  Xinming Sun  Biao Mu  Mingju Yu  Lingbing Wang 《Tropical animal health and production》2010,42(6):1257-1262

Two experiments were undertaken to select the efficient method applied for goat estrous synchronization. In experiment 1, a total of 120 does (Capra hircus) were divided into five groups with a randomized block design, and the does of treatment 1 were synchronized by intravaginal sponges impregnated with 30 mg Levonorgestrel inserted for 10 days. Does of treatments 2, 3, and 4 were treated with further injection of 25 IU follicle-stimulating hormone (FSH), 0.05 mg prostaglandin F2 alpha (PGF_2α), and 25 IU FSH + 0.05 mg PGF_2α at sponge withdrawal, respectively. The does in the control group (n = 40) without estrous synchronization treatment and natural estrous does were observed. In experiment 2, a total of 140 does in five goat farms in breeding and non-breeding seasons were treated with the selective efficient procedure. The results presented that all the employed treatments were capable of inducing and synchronizing estrous goats. According to estrous response and economy, the use of intravaginal sponges impregnated with 30 mg Levonorgestrel and 0.05 mg PGF_2α (treatment 3) is the first choice for estrous synchronization, and 95.0% of synchronized does demonstrated estrus, which was significantly higher than that of treatment 1 (P < 0.05) and control group (P < 0.01). The percentages of ovulating of treatments 3 and 4 were the same (95.0%), which were significantly higher than that of treatment 1 (P < 0.01). The ovulation rates among different groups were not significant (P > 0.05). When the selective procedure was applied to five goat farms, 85.7% (120/140) of does demonstrated estrus, and the kidding percentage, litter size, and prolificacy rate were 53.6%, 0.95, and 177%, respectively.  相似文献   

Effects of additive iron on growth,tissue distribution,haematology and immunology of gilthead sea bream, <Emphasis Type="Italic">Sparus aurata</Emphasis>     

George Rigos  Alexandros Samartzis  Morgane Henry  Eleni Fountoulaki  Efthimia Cotou  John Sweetman  Simon Davies  Ioannis Nengas 《Aquaculture International》2010,18(6):1093-1104

The comparative effects of iron-supplemented levels on growth, tissue distribution, haematology and immunology of gilthead sea bream, Sparus aurata (2 g) were investigated, using four organic (50, 100, 200, 300 mg ORG/kg diet) and one inorganic iron source (200 INOR mg/kg diet). Fish were treated for 12 weeks with the experimental diets and maintained at a water temperature of 19–22°C. Growth (final weight and specific growth rate), tissue distribution (spleen, liver and muscle), haematological parameters (red blood cells, haematocrit, haemoglobin and mean corpuscular haemoglobin concentration) and non-specific immune indexes (respiratory burst activity and antibacterial activity of serum) were analysed. No significant differences were found in growth and iron tissue distribution among the tested groups. Red blood cell counting was statistically higher in fish given 50 ORG, 100 ORG, 200 ORG and 200 INOR feeds. However, haematocrit, haemoglobin and mean corpuscular haemoglobin concentration were not significantly affected by increasing dietary iron. Fish receiving the 100 ORG diet had the best performance with respect to the respiratory burst activity and significantly higher values for antibacterial activity of serum were obtained in fish fed with the 300 ORG diet. The present findings provided no clear evidence of the optimum iron concentration. However, there was adequate indication that iron supplementation enhanced the performance of gilthead sea bream, mainly from a haematological and immunological point of view.  相似文献   

Pamela Walker: Growing good things to eat in Texas: Profiles of organic farmers and ranchers across the state     

Patrick T. Lillard 《Agriculture and Human Values》2010,27(4):527-528

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Relative parasitic fitness of isolates of Pyricularia oryzae Cav. with different sensitivities to fungicides     

Yukio Miyagi  Takashi Hirooka  Fujio Araki 《Pest management science》1986,17(6):653-658

The relative parasitic fitness of isolates of Pyricularia oryzae with different sensitivities to isoprothiolane (di-isopropyl 1,3-dithiolan-2-ylidenemalonate) and IBP (S-benzyl O, O-di-isopropyl phosphorothioate) was studied in the absence of fungicides. Four field isolates (S-1,S-2,MR-1 and MR-2) and two in-vitro mutants (Rvt-1 and Rvt-2) were used for disease epidemics. S-l and S-2 were wild types; MR-1 and MR-2 were sensitive to isoprothiolane and moderately resistant to IBP, and Rvt-1 and Rvt-2 were resistant to both fungicides. Twelve epidemics were made by inoculating rice seedlings with mixed conidial suspensions of two isolates or mutants. The value of relative parasitic fitness (W = 0-1.0) was calculated for each isolate and epidemic. S-l and S-2 were stronger (W = 1.0) than MR-2, Rvt-1 and Rvt-2; but weaker (W = 0.75, 0.73, respectively) than MR-1. MR-1 was strongest among all isolates and mutants used. MR-2 was slightly weaker (W = 0.9-1.0) than S-l and S-2, but stronger than Rvt-1 and Rvt-2. Rvt-1 and Rvt-2 had smaller values of W, ranging from 0.25-0.58, in the epidemics with each field isolate. These results suggest that the proportions of in-vitro mutants do not increase unless intensive selection pressure is given, and would be expected to decrease rapidly after the selection pressure is removed. Isolates moderately resistant to IBP, such as MR-1 and MR-2, however, had high values of W, suggesting that they would increase or would not decrease rapidly in the absence of selection pressure. These results may well explain why isolates highly resistant to isoprothiolane and IBP have seldom been found, and why a large number of isolates moderately resistant to IBP but sensitive to isoprothiolane have been observed in the field.  相似文献   

Isolation of Pathogenicity Mutants of Fusarium oxysporum f. sp. melonis by Insertional Mutagenesis     

lori INOUE  Toshiaki OHARA  Fumio NAMIKI  Takashi TSUGE 《Journal of General Plant Pathology》2001,67(3):191-199

Restriction enzyme-mediated integration (REMI) mutagenesis was used to isolate mutants of Fusarium oxysporum f. sp. melonis impaired in pathogenicity. The race 2 strain Mel02010 was transformed with linearized pSH75, conferring resistance to hygromycin B, with or without the enzyme used to linearize the plasmid. Addition of restriction enzymes did not affect the transformation frequency. A total of 2929 REMI transformants were tested for pathogenicity to three melon cultivars, Amus, Ogon 9 and Ohi. The race 2 strains are pathogenic to Amus and Ogon 9, but not to Ohi. Of 43 transformants with reduced pathogenicity on susceptible melon cultivars, 12 mutants were examined in detail for pathogenicity, vegetative growth and integrative mode of pSH75. The levels of pathogenicity varied among these mutants. Two mutants (B48 and B137) almost completely lost pathogenicity to both susceptible cultivars, and the others had reduced pathogenicity. Mutants B48, B241, B886 and X36 were also impaired in vegetative growth. Mutant B809 was a biotin auxotroph. By DNA gel blot analysis, nine mutants were found to contain a single copy of the transformation vector. These mutants may thus be useful in isolating genes involved in pathogenicity. Received 22 December 2000/ Accepted in revised form 16 April 2001  相似文献   

Mitotic disruption by a novel pyrimidine herbicide, NS-245852, in oat (Avena sativa L.) roots     

Akihiro Takahashi  Shigeo Yamada  Hirokazu Yamada  Takashi Kawana 《Weed Biology and Management》2001,1(3):182-188

The effects of a novel pyrimidine herbicide, NS-245852 [2-chloro-6-fluorophenyl-4-(trifluoromethyl)thieno[2,3-d]pyrimidine-2-yl-ketone], on mitosis in oat ( Avena sativa L. cv. Zenshin) root tips were investigated by using light and immunofluorescence microscopy. The root growth was strongly inhibited at 10⁻⁷ mol L⁻¹ of NS-245852, and swollen root tips were induced at 5 × 10⁻⁸ mol L⁻¹. As observed by the use of light microscopy, the herbicide produced disrupted mitosis and large polynucleate cells in the meristematic root tissue. These symptoms were similar to those of mitotic disrupter herbicides. The immunofluorescence microscopy studies of the root tip cells treated for 30 min revealed that spindle fibers and the preprophase band were reduced, although kinetochore fibers and the phragmoplast were not affected. Kinetochore fibers remained as small fluorescence spots, and the phragmoplast disappeared after a 3 h treatment. No microtubule arrays were observed by a longer treatment (longer than 3 h). Among the microtubule arrays, spindle fibers and the preprophase band were found to be the most sensitive to the herbicide, whereas kinetochore fibers were the most resistant. The phragmoplast was intermediate. Thus, the primary action of NS-245852 is the inhibition of polymerization of tubulin into microtubules.  相似文献