三联疫苗预防斑点叉尾(鱼回)细菌性疾病研究     

齐彩霞  张扬  裴家田  葛虹  陈霞  黄畛  万松良  葛雷 《水利渔业》2005,25(5):98-99

对斑点叉尾鮰致病的3株细菌,经液体培养后灭活制备三联免疫疫苗,斑点叉尾鮰鱼苗通过浸泡接种,抗感染能力大为增强.经人工感染试验,对CR79-1-1和ST78-3-3菌免疫保护率达到91％～100％，对93-Ⅲ菌免疫保护率可达64％。  相似文献   

海水池塘虾鱼贝蟹综合生态立体混养试验     

迟晓  黄佳祺  王学勃 《河北渔业》2007,(4):27-28

目前,全国的对虾池综合养殖面积已发展到20万hm2以上,养殖对虾的生态环境日益改善,与海区的水交换明显减少,暴发性虾病有所控制,对虾产量逐年回升,充分显示了虾池综合养殖强大的生命力和广阔的发展前景.  相似文献   

草鱼出血病的电镜观察   总被引：3，自引：0，他引：3  

郑德崇  黄琪琰  赵立勤  周孝康 《水产学报》1991,15(4):317-321

草鱼出血病是由呼肠孤病毒感染所致的急性传染病，由于病鱼小血管受损引起全身性出血，并造成内脏器官和体壁肌肉组织局灶性病变。电镜观察表明病变细胞内各种细胞器的数量较正常细胞显著减少，形态结构也遭损坏。质膜的三层结构模糊不清，及至解体消失。与机体代谢密切相关的粗面内质网、滑面内质网、线粒体和高尔基复合体的数量减少及结构受损，使蛋白质、糖类和脂质合成及分解等一系列细胞代谢活动不能正常进行，加速了病鱼死亡。  相似文献   

锦鲤暴发性出血症病原的研究   总被引：6，自引：0，他引：6  

莫介化  李春枝  张邦杰  黄永强  李本旺 《水产科技情报》2002,29(6):280-283

从患暴发性出症的锦鲤体内分离出2株细菌－052202和052203，应用细菌分离鉴定方法和法国Biomerieux FRC公司的API全自动微生物鉴定系统进行鉴定，结果显示：052202株为嗜麦芽窄食单胞菌（Stenotrophomonas maltophilia),052203株为温和气单胞（菌（Aeromonas sobria)。生物毒性试验的结果表明，温和气单胞菌可使健康锦鲤死亡，引起体表出血，肝，肾，脾，胆囊发生病变，有致病性；而嗜麦芽窄食单胞菌不引起健康锦鲤死亡，无致病性，28例药物的药敏试验结果表明，温和气单胞菌对新霉素，卡那霉素，庆大霉素，头孢噻吩，头孢曲松的头孢他啶的敏感性强或较强。  相似文献   

瓦氏黄颡鱼的生物学特性及亲本的引种、驯化和培育     

黄臻 《河南水产》2003,(2):22-23

瓦氏黄颡鱼分类隶属鲶形目,鱼危科,黄颡属,是我国长江中下游主要黄颡鱼品种之一,分布于长江中,洞庭湖、鄱阳湖也有少量分布。该鱼是黄颡鱼属中生长速度最快的品种。天然水体中三龄鱼,雄鱼可达400克以上,雌鱼200克以上。一般同龄鱼,雄鱼大于雌鱼。从长江中收集野生苗种人工养殖情况来看:该鱼比其它品种黄颡鱼容易捕捞,水温15℃以上,三网起捕率在85%以上。根据以上特点,我们将瓦氏黄颡鱼作为生产品种进行引种繁育,取得了一定的成效。下面就生物学特性及引种有关情况介绍如下。1瓦氏黄颡鱼的主要生物学特性1.1形态特征:体形长,胸腹部平坦,尾柄…  相似文献   

人工鱼礁建设实绩考察   总被引：6，自引：0，他引：6  

杨吝  刘同渝  黄汝堪 《现代渔业信息》2005,20(11):6-8,20

人工鱼礁建设已在世界各地盛行,已引起了世界的关注。作者就国外人工鱼礁建设实绩、国内人工鱼礁建设现状等专题作扼要概述。  相似文献   

锯缘青蟹亲蟹选择及培育技术     

夏连军  王建钢  施兆鸿  黄聪年 《海洋渔业》2003,25(4):189-191

亲蟹质量及亲蟹培育是锯缘青蟹人工育苗的基础。本文就亲蟹的选择、亲蟹暂养期的环境、亲蟹的饵料及饲养条件进行了探讨。在相同的理化因子条件下,海捕亲蟹的抱卵率(62.13％)大约为养殖亲蟹抱卵率(26.67％)的2.31倍。  相似文献   

Cryopreservation of marine microalgae and potential toxicity of cryoprotectants to the primary steps of the aquacultural food chain     

I. Tzovenis  G. Triantaphyllidis  X. Naihong  E. Chatzinikolaou  K. Papadopoulou  G. Xouri  T. Tafas 《Aquaculture (Amsterdam, Netherlands)》2004,230(1-4):457-473

Cryopreservation, a technique of high potential for culture collections, might offer a solution for reliable supply of microalgae in aquaculture units. Marine microalgae used in aquaculture were cryopreserved under 4, −20 and −80 °C using common cryoprotectants (methanol, dimethylsulfoxide (DMSO), propylene glycol and polyvinylpyrrolidone (PVP)) with promising results for Chlorella minutissima, Chlorella stigmatophora, Isochrysis galbana and Dunaliella tertiolecta. As cryoprotectants usually are toxic above certain concentrations and exposure time, and assuming that low amounts of cryoprotectants will remain in regenerated cultures, an experimental scheme was employed to explore the lower limits of safety for these algae and their primary consumers in hatchery food chains. Results showed that methanol was well tolerated by C. stigmatophora and D. tertiolecta up to a concentration of 1.6% (v/v) while I. galbana could not survive in culture at any concentration and C. minutissima exhibited some 30% of the control's yield at 0.2%. DMSO was highly tolerated up to 1.0% by all strains with the Chlorella strains surviving well up to 2%. Propylene glycol was not only tolerated up to 8% by Dunaliella but induced mixotrophic growth as well, while for Isochrysis it was lethal at any concentration. Among zooplanktonic consumers, brine shrimp Artemia nauplii could tolerate very high concentrations of the tested cryoprotectants, the rotifer Brachionus plicatilis was found sensitive to low amounts of PVP, while the nauplii of the shrimp Penaeus japonicus and the crab Eriocheir sinensis were in general very sensitive to all cryoprotectants and in several cases to much lower amounts than 1%. However, as long as the residues of cryoprotectants are kept below 1% in the regenerated cultures, there will be no problem with the animal consumers.  相似文献   

Experimental study on the effect of horizontal waves on netting panels   总被引：1，自引：0，他引：1  

Weihua Song  Zhenlin Liang  Hongfu Chi  Liuyi Huang  Fenfang Zhao  Lixin Zhu  Bohai Chen 《Fisheries Science》2006,72(5):967-976

The effect of horizontal waves on flexible netting panels is examined in this study, to evaluate the testing method of pretensioned mooring, radial systems, and flexible netting structures. The netting was suspended at a specific hanging ratio for six polyethylene panels with different characteristics. The aim was to evaluate the calculation method for horizontal wave forces on flexible netting panels. A regular wave was used in the experiment, with wave period 0.8–2.0 s and wave height 50–250 mm. The force on the netting structure was recorded by a tension transducer and digital signal recorder, and was simulated by a cubic spline in terms of the wave experiment under different wave conditions. The horizontal wave force on the netting panel changed periodically and asymmetrically, which was similar to the surface wave elevation. The horizontal wave force was related to the netting panel height and width (l), wave height (H) and wave length (L), twine diameter (d) and bar length (a) of the mesh. Using dual series relations, least-squares approximation, and multiple stepwise regression analysis, the following formula was obtained for the horizontal wave average apex value (F) on the netting panel: F=0.13ρgl(H/2)²(d/a)(H ^0.64 L ^0.77/0.9a ^0.44).  相似文献   

Optimal dietary protein requirement of grouper Epinephelus coioides juveniles fed isoenergetic diets in floating net cages   总被引：3，自引：0，他引：3  

Z. Luo  Y.J. Liu  K.S. Mai  L.X. Tian  D.H. Liu  & X.Y. Tan 《Aquaculture Nutrition》2004,10(4):247-252

An experiment to determine the optimal protein requirement of grouper Epinephelus coioides juveniles was conducted in floating net cages (1.5 m × 1 m × 1.5 m). Six isoenergetic fishmeal–casein‐based experimental diets containing 350–600 g kg^?1 crude protein (CP) were fed to triplicate groups of 20 fish (10.7 ± 0.2 g) for 56 days. Weight gain (WG) and specific growth rate (SGR) increased with increasing dietary protein level from 350 to 450 g kg^?1 and then plateaued above these levels. Feed intake (FI) showed no significant difference among fish fed more than 350 g kg^?1 CP. Lowest feed conversion ratio (FCR) was found for fish fed 500 g kg^?1 CP but this was not significantly different from that of fish fed the 450 and 600 g kg^?1 CP. Lowest protein efficiency ratio (PER) was found for fish fed 550 and 600 g kg^?1 CP. Fish fed the 600 g kg^?1 CP had the highest body protein and moisture contents but the lowest body lipid content. Body ash content was unaffected by protein level for fish fed >400 g kg^?1 CP. Dietary protein level had no significant effect on hepatosomatic index (HSI). Fish fed the 350 g kg^?1 CP had significantly lower condition factor (CF) and viscerosomatic index (VSI). Based on broken‐line regression analysis of SGR the optimal dietary protein requirement for E. coioides juveniles was determined to be close to 480 g kg^?1.  相似文献