犬瘟热病毒Guizhou株血凝素基因的克隆及序列分析   总被引：8，自引：0，他引：8  

朱军莉  余旭平  张登祥  沈琴芳  徐在平  李永明  周碧君  吴彤 《中国兽医学报》2006,26(1):17-20

参照已发表的文献合成1对引物，以犬瘟热病毒Guizhou分离株感染Vero细胞收获病毒提取的RNA为模板，RT-PCR扩增获得血凝素蛋白基因（H）901～1661位大小为761bp的片段，并将PCR扩增产物进行克隆和测序。将Guizhou株与GenBank数据库中的31株CDV及国内NanjingOP株的H基因序列进行同源性和系统发生树分析，发现Guizhou株的H基因部分序列与疫苗毒株Convac和Onderstpoort的同源性最低，只有90％；与日本野毒株Tanu96、KDK-1、Hmanatsu、Yanaka、UENO的同源性较高，为96％～98％；且与国内从大熊猫和小熊猫分离的野毒株GP和LP的同源性也较高，为98％和96％。系统发生树分析显示，Guizhou株与国内野毒株GP和LP，及日本的5个野毒株应属同一类，其中和GP株基因型最近，推测这些毒株间有更近的共同祖先。因此怀疑该病毒较适应于野生动物，且在我国某些地区的野生动物中可能存在自然疫源性传播。  相似文献   

菜薹无蜡粉性状遗传规律初探   总被引：3，自引：0，他引：3  

赵建锋  沈向群  张海楼  刘镜  蒋守义  刘同 《园艺学报》2006,33(3):538-538

菜薹（Brassica campestris L. ssp.chinensis var.utilis Tsen et Lee）又称菜心,是广东乃至华南地区主要蔬菜之一.其商品品质重要性状之一是叶和薹无蜡粉有光泽.本试验初步研究了菜薹蜡粉性状的遗传规律.  相似文献   

Effect of supernatants from cord blood mononuclear cells and peripheral blood mononuclear cells on infection of HIV-1 in vitro     

LI Hai-xian  ZENG Yao-ying  ZENG Xiang-feng  ZHAO Jing-xian  LI Xiao-jian  WANG Tong 《园艺学报》2006,22(4):638-641

AIM: To study the effect of supernatants from cord blood mononuclear cells (CBMC) and peripheral blood mononuclear cells (PBMC) on infection of HIV-1 in vitro, and develop effectively soluble factors for human acquired immunodeficiency syndrome (AIDS) treatment. METHODS: Different supernatants from CBMC and PBMC activated by PHA for 5 hours and 12 hours were added to cell culture systems between HIV-1ⅢB/H9 cells labeled by calcein-AM and MT-2 cells, then to count the fusion under a reverted fluorescent microscope after 2 hours, respectively, and different soluble factors in supernatants were detected by Luminex 100TM. RESULTS: These supernatants from CBMC and PBMC activated by PHA for 5 hours and 12 hours inhibited the formation of fusion, and there is no difference between supernatants collected in CBMC and PBMC at same time point. However, the supernatants collected in 5 hours were more effective to inhibit the formation of fusion than those in 12 hours (P<0.05). There was a lower concentration for pro-inflammatory cytokines, such as IFN-γ, MCP-1 and TNF-α, in supernatants from CBMC than those from PBMC, but the concentration of MIP-1a and RANTES in supernatants from CBMC was higher than those from PBMC. CONCLUSION: The soluble factors for anti-HIV can be prepared from CBMC and PBMC, which is a new source of anti-HIV therapeutic preparation.  相似文献   

《科技导报》（半月刊）欢迎订阅、欢迎投稿、欢迎刊登广告     

WANG Hui-ying  ZENG Yao-ying  WANG Tong  XING Fei-yue  ZHAO Jing-xian  JI Yu-hua 《园艺学报》2006,33(5):1020-1023

AIM: To study the effects of ultraviolet (UV) on mitochondrial functions and apoptosis in HaCaT cells. METHODS: After irradiation by UV at low dose (UVA 2 J/cm2，UVB 10 mJ/cm2) and high dose (UVA 6 J/cm2，UVB 30 mJ/cm2), HaCaT cells were cultured for 15 hours. Flow cytometry was used to measure mitochondrial membrane potential, mitochondrial mass and apoptotic rate. Annexin V-FITC/PI staining of apoptotic cells was analyzed by laser confocal microscopy. RESULTS: After UV irradiation, cell proportion with low mitochondrial membrane potential increased with irradiation doses. The proportion of control group, low dose group and high dose group were 7.94%±1.02%, 25.87%±4.55% and 39.27%±5.32%, respectively. Cells proportion with low mitochondrial mass increased with irradiation doses. The proportion of control group, low dose group and high dose group were 15.19%±1.58%, 40.36%±4.41% and 68.79%±5.46%, respectively. The hypodiploid peaks of DNA content analysis represented the apoptotic rate of HaCaT cells. The apoptotic rate of control group, low dose group and high dose group were 1.82%±0.51%, 30.16%±5.47% and 58.49%±5.98%, respectively. To analyze the cells apoptosis by staining with annexin V-FITC and PI, the results were consistent with those of DNA content analysis. Cells in control group showed almost no positive staining cells. Single annexin V-FITC positive cells in low dose group and double positive cells in high dose group were predominant, respectively. CONCLUSION: UV irradiation induces HaCaT cell mitochondrial depolarization, as well as mitochondrial mass loss. These changes are related to cell apoptosis.  相似文献   

油菜籽皮发酵饲料添加剂——纤维素酶     

邱雁临  胡婷婷  曾意 《饲料与畜牧》2007,(5):25-27

本研究对绿色木霉116的发酵条件和无机盐配方进行了正交试验优选。试验结果表明产纤维素酶的最佳条件是:接种量为2%,油菜籽皮:稻草:麸皮=3:2:5;水分:干料=1:1;装量为5%;无机盐固态发酵的最佳配方是:(NH4)2SO40.5%,KH2PO40.4%,MgSO40.04%,CaCl2·2H2O0.05%。产酶酶活可达242U/g鲜糟。  相似文献   

VEGF induces HUVECs to produce extracellular H₂O₂ and its proliferation role     

QIAN Zhong-qing  ZENG Yao-ying  WANG Tong  LIN Yi  JI Yu-hua  ZHAO Jing-xian 《园艺学报》2007,23(3):533-535

AIM:To study the effect of VEGF on extracellular H₂O₂ production in HUVECs and the role of H₂O₂ in the VEGF-induced proliferation. METHODS:HUVECs was stimulated with 500 μg/L VEGF. Products of extracellular H₂O₂ was detected by H₂DCFDA staining. MTT method was used to value the influences of 3×10⁶ U/L catalase and 5-20 mmol/L H₂O₂ to VEGF function. RESULTS:After treatment for 15 min with VEGF, HUVECs appeared fluorescence, and continued to become stronger, peaked at 45 min then decreased. HUVECs, which was treated simultaneity with VEGF and 3×10⁶ U/L catalase, only appeared very faint fluorescence. The proliferation of HUVECs by VEGF was restrained when treated with 3×10⁶ U/L catalase. The extrinsic H₂O₂ at concentration of 5-10 mmol/L promoted the proliferation of HUVECs but inhibited the proliferation effect of VEGF on HUVECs (P<0.01). CONCLUSION:These findings indicate that VEGF induces HUVECs to produce extracellular H₂O₂ and plays role in proliferation, but extrinsic H₂O₂ restrains VEGF function.  相似文献   

二温式多重RT-PCR同删两种主要兰花病毒的研究          下载免费PDF全文

郑国华明艳林  林德钦陈良华 范南星 《植物保护》2007,33(2):113-115

根据建兰花叶病毒(Cymbidium mosaic virus,Cy MV)和齿兰环斑病毒(Odontoglossum ringspot virus,ORSV)两种主要兰花病毒的保守序列设计特异性引物,建立了二温式多重RT-PCR同时检测两种病毒的检测方法。用该方法对感染两种病毒的植株总RNA模板进行扩增,结果同时得到2条大小与实验设计相符的769bp(Cy MV)、1000bp(ORSV)的特异性扩增带。用该方法对随机抽取的39个蝴蝶兰样品进行检测,结果11个样品检测出Cy MV,阳性率28.2%;24个样品检测出ORSV,阳性率61.5%;6个样品同时检测出两种病毒。  相似文献   

银杏种子UDPG焦磷酸化酶特性的研究     

于新  冯彤  庞杰  高全兴 《西南园艺》1998,(2)

银杏(Ginkgo biloba L.)种子中UDPG PPlase最适温度为29-30℃,最适pH为7.5-80,二价阳离子中Zn~(2 )、Ba~(2 )对其活性影响较小.Mn~(2 )、Mg~(2 )、Ca~(2 )对其有明显影响,而Cu~(2 )、Pb~(2 )有强烈的抑制作用.银杏种子幼小至膨大期,UDPG PPlase活性较低;种子成熟期至贮藏初期,活性迅速而显著地增强;贮藏过程中活性保持较高水平.  相似文献   

葡萄新品种--爱格丽   总被引：4，自引：0，他引：4  

李华  张振文  王华  刘延琳 《园艺学报》2000,27(1):75-75

以 3个欧亚葡萄栽培品种及其 2个中间杂交种为亲本 ,经过 15年培育出酿酒葡萄新品种‘爱格丽’ (Ecolly)。该品种除保持亲本的优质、丰产、抗寒性状以外 ,其最突出的特点是抗病性强 ,霜霉病病情指数为 16.5 ,是对照的 46.6%。适合酿造优质干白葡萄酒。  相似文献   

痣鳞鳃金龟的生物学特性与防治     

庞统  顾茂彬 《广东林业科技》2000,16(1):45-45,F003

痣鳞鳃金龟二年发生一代 ,此虫在砂质土壤的林地密度大 ,为害严重。造林时在每株根际施 5g 3 %呋喃丹颗粒剂 ,可有效地控制此虫的危害并兼治其它地下害虫和造林初期的食叶害虫。  相似文献