Effect of basal side-dressing of various types of coated urea fertilizer on shoot growth, yield components and seed composition of soybean (Glycine max (L.) Merr.)     

Yoshihiko TAKAHASHI  Norikuni OHTAKE  Makoto HATTORI  Yoshifumi NAGUMO  Takuji OHYAMA 《Soil Science and Plant Nutrition》2006,52(3):264-273

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Analysis of the nitrogen nutrition of soybean plants with deep placement of coated urea and lime nitrogen   总被引：1，自引：0，他引：1  

Kaushal TEWARI  Takanori SATO  Mayumi ABIKO  Norikuni OHTAKE  Kuni SUEYOSHI  Yoshihiko TAKAHASHI  Yoshifumi NAGUMO  Toru TUTIDA  Takuji OHYAMA 《Soil Science and Plant Nutrition》2007,53(6):772-781

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Enrichment of yeast thioredoxin by green tea extract through activation of Yap1 transcription factor in Saccharomyces cerevisiae     

Takatsume Y  Maeta K  Izawa S  Inoue Y 《Journal of agricultural and food chemistry》2005,53(2):332-337

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ECG and heart rate determination in fetal cattle using a digital signal processing method     

Wenxi CHEN  Tetsu NEMOTO  Toshio KOBAYASHI  Toshiyuki SAITO  Etsuko KASUYA  Yoshifumi HONDA 《Animal Science Journal》2002,73(6):545-551

A method of recording the fetal electrocardiogram (ECG) and heart rate (HR) is described. An abdominal lead and a thoracic lead were used to measure the fetal ECG and maternal ECG, respectively. The maternal component in the abdominal lead measurement was estimated by a digital adaptive filter using the thoracic lead measurement as a reference. By suppressing the estimated maternal components in the abdominal lead, the fetal ECG could be detected. The beat‐by‐beat fetal QRS complex peaks were determined by a digital matched filter from the fetal ECG enhanced in this way. The method was trialed in 10 pregnant Holstein cows at gestational periods ranging from 136 to 224 days. The results show that this method can extract the fetal ECG and determine the fetal HR from the raw noisy measurement data. The results suggest that the method would have applicability in monitoring fetal ECG as well as providing a non‐invasive, continuous HR profile during gestation, which will enable better understanding of the development of cattle fetuses before delivery.  相似文献   

Epidemiological survey of Theileria parasite infection of cattle in Northeast China by allele-specific PCR     

Yu L  Zhang S  Liang W  Jin C  Jia L  Luo Y  Li Y  Cao S  Yamagishi J  Nishikawa Y  Kawano S  Fujisaki K  Xuan X 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2011,73(11):1509-1512

An epidemiological survey on a Theileria parasite infection of cattle in Northeast China was carried out using allele-specific PCR and DNA sequence analysis of the major piroplasm surface protein (MPSP) gene. The results showed that 14 of 104 blood samples were positive for Theileria by PCR. Among the positive cases, co-infection with various combinations of C- and I-type parasites was detected in 12 samples; no B- and Thai-type parasites were detected by allele-specific PCR. Phylogenetic analysis based on the MPSP gene sequences revealed that Theileria parasites with the MPSP types 1, 2, and 4 were distributed in Northeast China.  相似文献   

桑蚕消化道中肠球菌的部分特性的研究     

鲁兴萌  钱永华  金伟  桥本义文  松本继男 《浙江大学学报(农业与生命科学版)》1997,(2)

在发现健康桑蚕幼虫消化道中存在β－溶血性的肠球菌的基础上，首次证实了肠球菌中只在厌氧条件下发生β－溶血反应菌的存在．Ｃｒａｉｇｉｅ试管法和毛细管法进一步确认了分离肠球菌菌株的生物性运动．“００８２”分离菌株在接种菌量较多、培养温度较高和碱性较大（ｐＨ值９～１０）条件下显示了较强的运动性．因此，它也将较容易地附着在桑蚕幼虫的围食膜上  相似文献   

Ontogenetic changes in RNA,DNA and protein contents of laboratory-reared Pacific bluefin tuna <Emphasis Type="Italic">Thunnus orientalis</Emphasis>     

Yosuke Tanaka  Woo-Seok Gwak  Masaru Tanaka  Yoshifumi Sawada  Tokihiko Okada  Shigeru Miyashita  Hidemi Kumai 《Fisheries Science》2007,73(2):378-384

ABSTRACT:     The ontogenetic changes in the growth potential of larval and juvenile laboratory-reared Pacific bluefin tuna were examined based on RNA–DNA and protein–DNA ratios. Experimental fish were reared at the Ohshima Experiment Station of Kinki University Fisheries Laboratory in August 2002. Samples were taken from 13 to 35 days after hatching (DAH). Metamorphosis from larva to the juvenile stage was observed around 23 DAH. Somatic growth of Pacific bluefin tuna was accelerated after metamorphosis. The value of the RNA–DNA ratio from 13 to 19 DAH increased slightly from 3.77 ± 0.58 (mean ± SD) to 7.28 ± 2.23. After that, the ratio markedly increased from 13.89 ± 3.71 on 21 DAH to 19.11 ± 4.27 on 23 DAH, which was the end of the metamorphic period. After 25 DAH, the ratio remained at a high level of 15–20. The protein–DNA ratio showed a similar tendency to the RNA–DNA ratio. These results suggest that the rapid increase in the RNA–DNA ratio in the metamorphic period supports the consequent rapid somatic growth in the juvenile stage. The high ratio after the metamorphic period could be because of the species-specific traits large prey exhibit for their survival and because of the tuna's fast -growth after the juvenile stage.  相似文献   

Testes maturation of reared Pacific bluefin tuna Thunnus orientalis at two-plus years old     

Yoshifumi Sawada  Manabu Seoka  Keitaro Kato  Toshihiro Tamura  Masahiro Nakatani  Shoji Hayashi  Tokihiko Okada  Kenichi Tose  Shigeru Miyashita  Osamu Murata  Hidemi Kumai 《Fisheries Science》2007,73(5):1070-1077

ABSTRACT:   Stable reproduction is essential for supplying artificially hatched fish to tuna aquaculture. We observed testes maturation in reared Pacific bluefin tuna (PBT) Thunnus orientalis at 2+ years of age. The incidence of males with mature testes was 25.0%, and 40% of the males had developing testes that contain spermatozoa, while oocytes of the same aged females were not mature. These fish were wild-caught at 0+ years old in August 1997 and the gonads were examined in October 1998 and January–February 2000. Therefore, the age at examination in 2000 was estimated to be 2 years and 7–10 months old considering the spawning season of the wild PBT and the size when captured. Histological examination of the matured and developing testes showed that they contained spermatozoa, spermatids, spermatocytes, and spermatogonia. All the spermatozoa were observed to be motile in sea water under light microscopy. From the results of this and previous studies, matured males are probably fertile for at least 5 months a year in Kushimoto. The testes maturation observed at young age in captivity is considered promising to reduce the cost of broodstock maintenance for the juvenile production of PBT, especially if the sperm are cryopreserved.  相似文献   

Effect of fasting on physical/chemical properties of ordinary muscles in full-cycle cultured Pacific bluefin tuna Thunnus orientalis during chilled storage   总被引：1，自引：0，他引：1  

Yoshi-Nori Nakamura  Masashi Ando  Manabu Seoka  Ken-Ichi Kawasaki  Yoshifumi Sawada  Shigeru Miyashita  Tokihiko Okada  Hidemi Kumai  Yasuyuki Tsukamasa 《Fisheries Science》2006,72(5):1079-1085

Using the full-cycle cultured (FC) Pacific bluefin tuna [body weight 16.3±1.9 kg (pre-fasting group, pre-FG), 14.2±0.9 kg (post-fasting group, post-FG)], changes in the physical/chemical properties of the cephalal parts of dorsal (D) and ventral (V) ordinary muscles (OM) by fasting (6 days) during chilled storage (4°C) were investigated. Condition factors were 26.7 (pre-FG) and 20.3 (post-FG, P < 0.05). Fasting changed the liver color to green. Fasting also decreased the amount of protein and lipid contents of the DOM and VOM of FC tuna. The breaking strength and pH of the DOM and VOM of post-FG tuna were higher (P <0.05) than for pre-FG tuna during storage. In contrast, the glycogen contents of DOM and VOM of post-FG tuna were lower than for pre-FG tuna. The color values (L*, a* and b*) of DOM of post-FG tuna were lower than for pre-FG tuna throughout the storage period. In addition, the metmyoglobin (metMb) content of DOM of post-FG tuna was lower (P <0.05) than that of pre-FG tuna, and the metMb content of VOM of post-FG tuna remained low after fasting. These results indicate that fasting suppresses deterioration (especially meat color) of FC tuna muscles during chilled storage.  相似文献   

ULTRASONOGRAPHIC FINDINGS RELATED TO PROGNOSIS IN CANINE TRANSITIONAL CELL CARCINOMA     

Kiwamu Hanazono  Shinya Fukumoto  Yoshifumi Endo  Hiroshi Ueno  Tsuyoshi Kadosawa  Tsuyoshi Uchide 《Veterinary radiology & ultrasound》2014,55(1):79-84

In human bladder cancer patients, ultrasonography is extensively used not only to identify tumor masses but also to evaluate tumor size, shape, echogenicity, location, and degree of tumor invasion into the bladder wall. The information revealed by ultrasonography delineates the tumor's biological features and facilitates prediction of prognosis. However, in veterinary medicine the feasibility of using ultrasonography for these purposes has not been fully investigated. In this retrospective study, we reviewed cases of dogs with histologically confirmed bladder mass lesions, including transitional cell carcinoma (n = 22) and polypoid cystitis (n = 5), to determine whether ultrasonography could reliably predict bladder wall involvement. By following patients with transitional cell carcinoma until death, we also determined whether ultrasonographic tumor size, shape, echogenicity, and mass location were related to prognosis. Wall involvement as revealed by ultrasound was significantly (P = 0.00005) associated with histological muscular layer involvement with a sensitivity of 93% (95% Confidence interval, 79–98%) and specificity of 92% (95% Confidence interval, 76–98%). Ultrasonographic wall involvement (P = 0.03, vs. noninvolvement), heterogeneous mass (P = 0.02, vs. homogeneous mass), and trigone location (P = 0.01, vs. other locations) characteristics were significantly associated with shorter survival times in transitional cell carcinoma cases. Findings indicated that ultrasonographic characteristics such as wall involvement, heterogeneous mass, and trigone location could be reliable prognostic indicators in canine transitional cell carcinoma.  相似文献