甘蓝与萝卜属间杂交及其胚挽救技术优化     

扈新民  李锡香  梁燕  方智远  沈镝  邱杨 《中国蔬菜》2009,1(10):7-12

以甘蓝显性雄性不育系C08-11为母本，以萝卜自交系Q07-12为父本进行属间杂交，对杂交子房和胚珠进行离体培养，筛选适合甘蓝与萝卜属间杂种胚挽救的适宜取材时间、培养基及外源添加物。结果表明，胚珠培养优于子房培养；所有处理子房培养仅获得3株杂交苗，取材时间以授粉后第9天为宜，B5培养基中添加合适的生长调节剂、水解酪蛋白和活性炭为子房培养所必需；胚珠培养各处理共获得29株杂交苗，适宜取材时间为蕾期授粉后第14天、花期授粉后第16天，对生长调节剂的需求取决于不同取材时期及培养基。  相似文献   

鼠李糖制备新工艺研究   总被引：2，自引：0，他引：2  

王秀军  高锦明  李晓明  张鞍灵 《西北农业学报》2006,15(6):183-185

采用正交设计方法探讨发酵法从芦丁水解液里提取制备鼠李糖的最佳制备工艺,优选出鼠李糖纯度达到99%以上的最佳制备工艺为:温度30℃,pH值5.0,酵母量为母液的2%。  相似文献   

论职务犯罪黑数     

孙昌军  庄慧鑫 《湖南农业大学学报(自然科学版)》2003,(5)

职务犯罪黑数是指已经实际发生但司法机关尚未发现或未作处理的职务犯罪数 ,我国职务犯罪黑数较大是不争的事实 ,职务犯罪的内在特征与法制环境的消极方面是导致这一现象的深层原因 ,应当认识到职务犯罪黑数的社会危害性与一般规律 ,采取相应措施遏制其增长。  相似文献   

试论工科院校大学生的审美教育     

李道先  侯曙芳 《安徽农业大学学报》2004,(6):81-84

工科院校在强调专业教育的同时，没能同样重视审美教育。加强对工科院校大学生的审美教育，有助于培养正确的审美观，提高审美能力;有助于完善知识结构，提升人文精神; 有助于形成独立完善的人格，提升人格境界;有助于培养个性和谐、德智体美全而发展的高素质人才。  相似文献   

S1核酸酶突变检测法的优化     

李春雨 王涛梁本国  李光晨 《勤云标准版测试》2004,(5)

优化了S1核酸酶突变检测体系,结果表明(1)扩增法比杂交法产生异源双链DNA更节省时间;(2)PCR产物可以直接作为突变检测的底物;(3)适当降低反应温度、适当增加反应缓冲液中的NaCl浓度、适当缩短反应时间可提高突变检测效果。  相似文献   

大白菜耐热性遗传效应研究   总被引：19，自引：2，他引：17  

吴国胜  王永健 《园艺学报》1997,24(2):141-144

以两组耐热和热敏感的大白菜对应亲本（１７７与２７６；１７６与２７９）及其杂交、回交世代（Ｆ１、Ｆ２、Ｂ１、Ｂ２）为材料进行耐热性遗传模型分析和遗传参数估算，得到一致的结果：大白菜的耐热性符合加性－显性－上位性模型，以加性遗传效应为主，兼有上位性效应，显性效应不显著。广义遗传力和狭义遗传力均较高。以耐热和热敏感的５个代表性亲本材料采用Ｇｒｉｆｉｎｇ的双列杂交方法Ⅱ配制杂交组合进行配合力分析，结果表明：加性遗传方差组分很高，而显性遗传方差组分很小，与上述结果一致。同时，耐热亲本材料１７６和１７７均有较高的一般配合力效应值，是耐热性选育的较好亲本材料。  相似文献   

益生素预防畜禽腹泻机理的研究   总被引：7，自引：0，他引：7  

师守信  廉晓华 《黑龙江畜牧兽医》1994,(2):3-5

用革兰氏阳性的嗜酸乳杆菌和枯草杆菌的活菌和死菌喂饲了８０只雏鸡，用嗜酸乳杆菌的活菌和死菌喂饲了３７头仔猪和２４头犊牛，观察这些细菌防畜禽腹泻的效果。结果证明这两种细菌的活菌与死菌都有预防腹泻的功能，嗜酸乳杆菌的效果好于枯草杆菌。两种菌的活菌和死菌都有预防作用说明是菌体成分或代谢产物可能是预防腹泻的主要有效成分。  相似文献   

桃需冷量遗传特性的研究     

王力荣  朱更瑞  左覃元 《果树学报》1996,(4)

对桃的９个杂交组合４６３株杂种实生苗需冷量进行测定,统计结果表明：桃需冷量是由多基因控制的,Ｆ＿１需冷量平均值较亲中值短,不同组合偏离程度不同,南山甜桃、红日、玛丽维拉、白凤、大久保需冷量育种值分别为１５９、２１６、３１９、８３１、８３４ｈ,遗传传递力分别为７９%、５４%、９２%、８３%、９３%。需冷量的遗传力为８９．６９%。萌芽开花与需冷量密切相关。  相似文献   

Factors influencing decline in soil pH in Hawaiian Eucalyptus and Albizia plantations     

C. Rhoades  D. Binkley 《Forest Ecology and Management》1996,80(1-3):47-56

Soil pH declined from 5.9 to 5.0 in 8 years beneath plantations of Eucalyptus saligna (Sm.) in Hawaii. In stands of Albizia falcataria, (L.) Fosberg, the soil pH change was more dramatic, declining from 5.9 to 4.6. We measured several components of soil acidity beneath four mixtures of the two tree species to gain insight on the processes responsible for the decline in soil pH. These components were studied using an empirical method of comparing acid quantity, degree of neutralization (depletion of base cations), and acid strength. The decline in soil pH differed between species as a result of differences in the degree of neutralization of the soil exchange complex; the larger decrease in soil pH under Albizia was produced by greater acidification of the exchange complex. Empirical titration curves suggested that differences in acid strength moderated the divergence in soil pH beneath the species. Had the acids accumulating in the soil under Albizia been as strong as those in the Eucalyptus soil, the difference in soil pH would have been greater. Though the two species had contrasting effects on soil pH, the differences in degree of neutralization, responsible for the pH decline, were small compared with differences in the amount of cations stored in tree biomass. Continued supply of nutrient cations (from weathering or fertilization) will ultimately control both the extent of soil pH decline and the level of productivity sustained by the forest.  相似文献   

Monoclonal antibodies to bovine viral diarrhea virus: cross-reactivities to field isolates and hog cholera virus strains.   总被引：2，自引：1，他引：1       下载免费PDF全文

D Deregt  S R Bolin  R A Heckert    K G Loewen 《Canadian journal of veterinary research》1994,58(1):71-74

Monoclonal antibodies to bovine viral diarrhea virus (BVDV) were examined for binding with a large number of North American BVDV isolates and eight strains of the serologically related pestivirus, hog cholera virus (HCV). No single BVDV monoclonal antibody reacted with all BVDV isolates. The most cross-reactive monoclonal antibody was an anti-p80/p125 antibody which showed a positive reaction with 173 of 180 (96%) North American isolates. From a fewer number of isolates tested, one anti-gp53 monoclonal antibody also showed a high cross-reactivity (94%). All BVDV isolates showed a positive reaction with at least one of the seven monoclonal antibodies in the panel. Thus, the results indicated that a pool of these monoclonal antibodies may be used in place of polyclonal antisera for the detection of BVDV contamination of cell lines or for virus isolation. For HCV, all three anti-p80/p125 monoclonal antibodies reacted positively with all eight virus strains. In contrast, none of the anti-gp53 monoclonal antibodies were reactive to HCV strains. Thus, the anti-gp53 monoclonal antibodies may be useful for distinguishing between usually innocuous BVDV infections and the highly significant HCV infections in swine for foreign animal disease surveillance.  相似文献