Comparison of in vitro vitellogenin synthesis among different nonylphenol products using primary cultures of tilapia hepatocytes   总被引：1，自引：0，他引：1  

BYUNG Ho KIM  AKIHIRO TAKEMURA  MASARU NAKAMURA 《Fisheries Science》2002,68(4):838-842

Effect of nonylphenol (NP) products from different companies on in vitro vitellogenin (VTG) synthesis was compared using tilapia ( Oreochromis mossambicus ) hepatocytes cultures. Addition of NP at a concentration of 10⁻³ M to the medium caused death of hepatocytes (NP-A and NP-B) and delay of monolayer formation (NP-C). No cell death was observed at a concentration of 10⁻⁴ M (NP-A, -B and -C) but cell adhesion was slower than control. These results suggest that high concentration of NP is toxic against tilapia hepatocytes. When effects of estradiol-17β (E₂, 10⁻⁷ M) and NP (10⁻⁴ M) on in vitro VTG synthesis were examined, addition of E₂ and NP-A and NP-B to the media resulted in elevation of VTG. NP-C did not induce VTG in the medium. Co-treatment of NP-B and tamoxifen, a non-steroidal anti-estrogen, reduced VTG synthesis. These results suggest that NP has estrogenic potential in primary cultures of tilapia hepatocytes and acts through binding to the estrogen receptor, and that there is a difference in the induction level of VTG among different NP products.  相似文献   

Genetic differentiation among three color variants of Japanese sea cucumber Stichopus japonicus   总被引：6，自引：0，他引：6  

Manami  KAN-NO  Akihiro  KIJIMA 《Fisheries Science》2003,69(4):806-812

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Genetic information of three pure lines of Porphyra yezoensis (Bangiales, Rhodophyta) obtained by AFLP analysis   总被引：5，自引：0，他引：5  

OSAMU  IITSUKA  KAYO  NAKAMURA  AKIYUKI  OZAKI  NOBUAKI  OKAMOTO  NAOTSUNE  SAGA 《Fisheries Science》2002,68(5):1113-1117

Porphyra (Bangiales, Rhodophyta), which includes several valuable marine crops, has recently received great interest as a model plant for fundamental and applied studies in marine sciences. Amplified fragment length polymorphisms (AFLPs) are a robust and efficient means for genetic mapping, linkage analysis of genetic characters for breeding and population studies in land plant genomes. To examine whether AFLPs are applicable as genetic markers in the present study, we detected AFLP markers with three pure lines in order to promote genetic analysis in Porphyra yezoensis . The following five sets of AFLP primer pairs (E-AA, M-CAA) (E-AA, M-CAC) (E-AA, M-CAG) (E-AA, M-CAT) (E-AA, M-CTA) were tested with template DNAs from three pure lines and they showed a total of 227 bands. This suggests that AFLP markers are promising tools for genetic analysis in Porphyra .  相似文献   

Infection of tilapia, Oreochromis mossambicus (Trewavas), by a marine monogenean, Neobenedenia melleni (MacCallum, 1927) Yamaguti, 1963 in Kaneohe Bay, Hawaii, USA, and its treatment   总被引：2，自引：0，他引：2  

J. J. KANEKO II  R. YAMADA  J. A. BROCK  R. M. NAKAMURA 《Journal of fish diseases》1988,11(4):295-300

Abstract A disease of saltwater, cage-cultured tilapia, Oreochromis mossambicus (Trewavas), caused by the marine monogenean, Neobenedenia melleni (MacCallum, 1927) Yamaguti, 1963, is described. Up to 400 parasites were found attached to the body surface of individual fish. Heavily infected fish showed hyperirritability, heavy mucus secretion and discoloration. Pathology was most marked on the eye, with corneal opacity initially, followed by buphthalmos, corneal ulceration and rupture of the eye with subsequent degeneration of internal structure. The infection was successfully treated using 2 min freshwater dips.  相似文献   

Evaluation of serum and urine 1,5-anhydro-D-glucitol and myo-inositol concentrations in healthy dogs     

Miki Y  Mori A  Hayakawa N  Niki T  Oda H  Saeki K  Sato T  Tazaki H  Ishioka K  Arai T  Sako T 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2011,73(9):1117-1126

1,5-anhydro-D-glucitol (1,5AG) is a pyranoid polyol compound found in human circulating blood. Myo-inositol (MI) is a stereoisomer of inositol and serves as a precursor of inositol phospholipids. 1,5AG and MI are filtered by the glomerulus and almost completely reabsorbed through the renal tubules. However, under hyperglycemic conditions, reabsorption through the renal tubules is competitively inhibited because the structures of 1,5AG and MI resemble that of glucose. In this study, we investigated the kinetics of serum and urine 1,5AG and MI levels in healthy dogs. We demonstrated that 1,5AG and MI exist in canine serum and urine by gas chromatography-mass spectrometry. Under continuous hyperglycemic conditions, the serum 1,5AG concentration in healthy dogs decreased while the serum MI concentration remained unchanged. Urinary excretion of 1,5AG and MI increased significantly after blood glucose concentrations reached 200 to 220 mg/dl. A significant negative correlation was observed between serum 1,5AG and glucose concentrations during hyperglycemia. However, no significant correlation was observed between serum MI and glucose concentrations. In this study, we demonstrated that serum and urine 1,5AG and MI levels were changed by blood glucose concentrations. The serum 1,5AG concentration was decreased by continuous hyperglycemia. However, the serum MI concentration does not reflect hyperglycemia.  相似文献   

Cerebral vascular hamartoma with thrombosis in a dog     

Sakurai M  Morita T  Kondo H  Uemura T  Haruna A  Shimada A 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2011,73(10):1367-1369

A cerebral vascular hamartoma was identified in the frontal lobe, striatum and thalamus of the right side of the brain of a male, 7-year-old Shih Tzu. Histologically, the lesion consisted of thin-walled vessels, which showed various sizes and occasionally contained fibrin thrombi. These vascular walls were composed of a single layer of fibromuscular tissue lined by flat endothelium with various amount of collagen, but devoid of large coat of smooth muscles and elastic tissue. Immunohistochemically, the lining endothelial cells were positive for von Willebrand Factor antibody. Neuropil between the vessels was stained with Klüver-Barrera stain, and positive for synaptophysin and GFAP antibodies. Based on these findings, the lesion was diagnosed as vascular hamartoma, which might resemble venous malformation in humans.  相似文献   

Behavioral indexes of piglet welfare: Comparison of indoor and outdoor housing systems     

Kouzo NAKAMURA  Toshio TANAKA  Kouji NISHIDA  Katsuji UETAKE 《Animal Science Journal》2011,82(1):161-168

The purpose of this experiment was to establish behavioral indexes of piglet welfare. Forty‐eight piglets were allocated to either four indoor pens or four outdoor pens (six piglets per pen). The indoor system was a commercial pen that consisted of a concrete floor and a slat floor. The outdoor system had a dirt paddock with a wooden hutch. Growth performance, salivary cortisol levels, skin lesions and behaviors of the piglets were monitored for 4 weeks. Sixteen types of behaviors were recorded by using 2‐min instantaneous scan sampling for 8 h/day. Growth performance and salivary cortisol levels did not significantly differ between the two housing systems. On the other hand, skin lesions and behaviors were significantly affected by the housing system. The number of skin lesions was higher in the indoor system. In addition, piglets in the outdoor system showed more investigative and social‐play behaviors than those in the indoor system. Piglets in the indoor system showed more resting, drinking, moving, fighting, and conflict behaviors than those in the outdoor system. We conclude that investigative, social‐play and conflict behaviors may be effective indexes of the welfare level of piglets, especially investigative and conflict behaviors.  相似文献   

Molecular cloning, nucleotide sequence and characteristics of a xylanase gene (xynA) from Ruminococcus albus 7     

Mutsumi NAKAMURA  Takafumi NAGAMINE  Akio TAKENAKA  Rustem I. AMINOV  Koretsugu OGATA  Kiyoshi TAJIMA  Hiroki MATSUI  Yoshimi BENNO  Hisao ITABASHI 《Animal Science Journal》2002,73(5):347-352

A 2.6-kb DNA fragment encoding a xylanase gene ( xyn A) was cloned from the rumen hemicellulolytic bacterium Ruminococcus albus 7. The deduced primary structure of the protein (XynA) was divided into a signal peptide region and 3 domains. Domain A was identified as a family 11 (G) catalytic domain, but one amino acid residue was replaced by another in an active site signature 1 of family 11. Domain B is a stabilizing domain for the catalytic domains of families 10 and 11. Deletion of domain B reduced stability of the xylanase at high temperature and at high and low pH. Domain B may be useful for protein engineering of xylanase. Domain C has sequence similarity to deacetylases and NodB proteins.  相似文献   

Effect of administration of acid‐ and bile‐tolerant lactobacilli on pigs intestinal microbial population     

Makoto OTSUKA  Tomoko MUTO  Yu KATSUNUMA  Yutaka NAKAMURA  Ryozo TAKADA  Hajime MINATO 《Animal Science Journal》2002,73(6):497-503

The effects of the administration of four Lactobacillus strains as probiotics on the constitution of microbial populations in the intestine of pigs were investigated by using a litter of pigs. The experimental group was fed a diet blended with the freeze‐dried powder containing lactobacilli at a concentration of 0.2% (W/W). After 4 weeks of probiotics administration, the total viable count of anaerobes in the feces was not significantly different between the pigs fed a diet supplemented with probiotics and the pigs given the non‐supplemented diet. However, viable counts of eubacteria were significantly higher (P < 0.01) in the feces of pigs fed a diet supplemented with probiotics than in the pigs given the non‐supplemented diet. Conversely, viable counts of clostridia were significantly lower (P < 0.01) in the feces of pigs fed a diet supplemented with probiotics than the pigs given the non‐supplemented diet. The total volatile fatty acid concentrations in the feces of pigs that were given a diet supplemented with probiotics were significantly lower (P < 0.05) than those given the non‐supplemented diet. Further, the molar proportion of n‐butyric acids in the feces of pigs that were given a diet supplemented with probiotics, was significantly lower (P < 0.05) than those given the non‐supplemented diet.  相似文献   

The effects of medetomidine and xylazine on gastrointestinal motility and gastrin release in the dog     

K. NAKAMURA  S. HARA  & N. TOMIZAWA 《Journal of veterinary pharmacology and therapeutics》1997,20(4):290-295

The effect of medetomidine, a potent and highly selective α₂-adrenoceptor agonist, on the motility of the gastric antrum, duodenum, mid-jejunum and ileum was investigated in ten dogs. Its effect on the release of gastrin was also determined. Administration of medetomidine intramuscularly (i.m.) at a dose of 40 μg/kg inhibited the motility of the gastric antrum, duodenum, mid-jejunum and ileum significantly, in comparison to administration of xylazine intramuscularly at a dose of 2.0 mg/kg. The release of gastrin was also significantly decreased in dogs receiving medetomidine. It was found to inhibit the motility in the gastric antrum and duodenum longer than in the mid-jejunum and ileum, presumably by acting specifically on α₂-adrenoceptors, likely at the peripheral level. Medetomidine also inhibited the gastric contraction associated with gastrin secretion.  相似文献