A case of acute distal cyanosis during percutaneous catheterization of the dorsal metatarsal artery in a goose (Anser anser)          下载免费PDF全文

Samanta Waxman  Andrea S Zaccagnini  Santiago Fuensalida  Pablo Regner  Pablo E Otero 《Veterinary anaesthesia and analgesia》2015,42(5):557-558

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Assessment of coagulation utilizing thromboelastometry in dogs undergoing orthopedic surgery          下载免费PDF全文

Barbara Bruno DVM  Cristiana Maurella DVM  Sara Falco DVM  Alberto Tarducci DVM  Renato Zanatta DVM  PhD  Paola Gianella DVM  PhD  DACVIM  Antonio D'Angelo DVM  PhD  DECVN  DECBHM  Lisa Piras DVM  PhD  Andrea Di Bella DVM  MRCVS  Antonio Borrelli DVM  PhD  MS 《Journal of Veterinary Emergency and Critical Care》2015,25(3):358-363

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Changes in adherence to cardiopulmonary resuscitation guidelines in a single referral center from January 2009 to June 2013 and assessment of factors contributing to the observed changes          下载免费PDF全文

Andrea C. Holmes BSc  BVSc  Louise Clark BVMS  DECVAA 《Journal of Veterinary Emergency and Critical Care》2015,25(6):801-804

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A microwave tomographic system for wood characterization in the forest products industry     

Matteo Pastorino  Alessandro Fedeli  Andrea Salvadè  Samuel Poretti  Manuela Maffongelli 《Wood material science & engineering》2015,10(1):75-85

Abstract

Nondestructive testing and evaluation techniques able to extract information about the internal structure of the samples under test are very important in the wood industry. Microwave imaging systems have been considered for a long time promising apparatuses for this task. In this framework, approaches exploiting the full scattering phenomena for creating images of the distributions of the dielectric properties of the targets have been developed in the last few years. In this paper, a prototype of microwave tomographic system is presented and several experimental validation confirming its suitability for the use in the wood and forest product industry are reported.  相似文献   

Identification of PCR-amplified genetically modified organisms (GMOs) DNA by peptide nucleic acid (PNA) probes in anion-exchange chromatographic analysis     

Rossi S  Lesignoli F  Germini A  Faccini A  Sforza S  Corradini R  Marchelli R 《Journal of agricultural and food chemistry》2007,55(7):2509-2516

PCR products obtained by selective amplification of transgenic DNA derived from food samples containing Roundup Ready soybean or Bt-176 maize have been analyzed by anion-exchange HPLC. Peptide nucleic acids (PNAs), oligonucleotide analogues known to bind to complementary single-stranded DNA with high affinity and specificity, have been used as specific probes in order to assess the identity of the peaks observed. Two different protocols were adopted in order to obtain single-stranded DNA: amplification with an excess of one primer or digestion of one DNA strand. The single-stranded DNA was mixed with the PNA probe, and the presence of a specific sequence was revealed through detection of the corresponding PNA:DNA peak with significantly different retention time. Advantages and limits of this approach are discussed. The method was tested with reference materials and subsequently applied to commercial samples.  相似文献   

Formation of hydroxyphenyl-pyranoanthocyanins in Grenache wines: precursor levels and evolution during aging     

Rentzsch M  Schwarz M  Winterhalter P  Hermosín-Gutiérrez I 《Journal of agricultural and food chemistry》2007,55(12):4883-4888

Grenache red wines were produced following three different winemaking techniques, that is, small-scale standard experimental wines and industrial-scale wines by both double-mash fermentation and fermentation in Ganimede vats. Wines were analyzed for their color properties, as well as the anthocyanin, flavonol, hydroxycinnamic acid, and pyranoanthocyanin profiles following alcoholic and malolactic fermentation. The evolution of pyranoanthocyanins and their corresponding precursors in the experimental wines was monitored at 6 and 10 months of aging. Wines produced by double-mash fermentation exhibited superior color properties compared to Ganimede wines and the experimental red wines, due to better extraction of flavonols and anthocyanins as well as a lower degree of polymerization. Pyranoanthocyanin formation varied within the different classes of pigments. Vitisins A and B were formed only during alcoholic fermentation. Pinotin A (i.e., the reaction product from malvidin 3-glucoside and caffeic acid) formation took place only during the aging process, whereas formation of hydroxyphenyl-pyranoanthocyanins derived from p-coumaric and ferulic acid followed two different pathways, that is, an enzymatically assisted production during fermentation and a pure chemical formation during aging.  相似文献   

Validation of a real-time PCR for the quantitative estimation of a G143A mutation in the cytochrome bc1 gene of Pyrenophora teres     

Kianianmomeni A  Schwarz G  Felsenstein FG  Wenzel G 《Pest management science》2007,63(3):219-224

A single nucleotide polymorphism (SNP) in the cytochrome b gene confers resistance to strobilurin fungicides for several fungal pathogens. Therefore, on the basis of a change at amino acid position 143 from glycine to alanine, a real-time PCR assay was established for the quantitative detection of the analogous SNP in the cytochrome b sequence of Pyrenophora teres Drechsler, which causes barley net blotch. Allelic discrimination was achieved by using allele specific primers with artificially mismatched nucleic acid bases and minor groove binding probes. Validation parameters for the lower limits of the working range, namely limits of detection (LOD) and limits of quantification (LOQ), were statistically determined by the variance of calibration data, as well as by the variance of the 100% non-strobilurin-resistant allele DNA sample (blank values). It was found that the detection was limited by the variance of blank values (five in 801 458 copies; 0.0006%), whereas the quantification was limited by the variance of calibration data (37 in 801 458 copies; 0.0046%). The real-time PCR assay was finally used to monitor strobilurin-resistant cytochrome b alleles in barley net blotch field samples, which were already classified in in vivo biotests to be fully sensitive to strobilurins. All signals for strobilurin-resistant cytochrome b alleles were below the LOD, and therefore the results are in total agreement with the phenotypes revealed by biotests.  相似文献   

Association of key foods and beverages with obesity in Australian schoolchildren   总被引：4，自引：0，他引：4  

Sanigorski AM  Bell AC  Swinburn BA 《Public health nutrition》2007,10(2):152-157

OBJECTIVE: To examine the pattern of intake of key foods and beverages of children aged 4-12 years and the association with weight status. DESIGN AND SETTING: A computer-assisted telephone interview was used to determine the intake of fruit, vegetables, packaged snacks, fast foods and sweetened drinks 'yesterday' and 'usually' as reported by parents/guardians of a representative sample of 2184 children from the Barwon South-Western region of Victoria, Australia. RESULTS: Children who consumed >2-3, >3-4 and >4 servings of fruit juice/drinks 'yesterday' were, respectively, 1.7 (95% confidence interval (CI) 1.2-2.2), 1.7 (95% CI 1.2-2.5) and 2.1 (95% CI 1.5-2.9) times more likely to be overweight/obese compared with those who had no servings of fruit juice/drink 'yesterday', adjusted for age, gender and socio-economic status (SES). Further, children who had > or = 3 servings of soft drink 'yesterday' were 2.2 (95% CI 1.3-3.9) times more likely to be overweight/obese compared with those who had no servings of soft drink 'yesterday', adjusted for age, gender and SES. In addition, children who 'usually' drank fruit juice/drinks twice or more per day were 1.7 (95% CI 1.2-2.4) times more likely to be overweight/obese compared with those who drank these beverages once or less per week, adjusted for age, gender and SES. Although fast foods and packaged snacks were regularly eaten, there were no associations between weight status and consumption of these foods. CONCLUSIONS: Intake of sweetened beverages was associated with overweight and obesity in this population of Australian schoolchildren and should be a target for intervention programmes aimed at preventing unhealthy weight gain in children.  相似文献   

Fish feed can show genotoxic damage     

Ota  Erika do Carmo  Cardoso  Claudia Andrea Lima  Inoue  Luis Antonio Kioshi Aoki  Zanon  Ricardo Basso  Silva  Tarcila Souza de Castro 《Fish physiology and biochemistry》2022,48(3):735-748

Fish Physiology and Biochemistry - The present study was carried out to evaluate the genotoxic potential of nutritional quality of feed, using erythrocytic nuclear abnormalities assay in Nile...  相似文献   

Comparison of 3 methods of onychectomy     

Kyle Clark  Trina Bailey  Paul Rist  Andrea Matthews 《The Canadian veterinary journal. La revue veterinaire canadienne》2014,55(3):255-262

The frequency of claw regrowth, bony remnants, and complications in cats that underwent forelimb onychectomy using laser, scalpel, or guillotine techniques were evaluated. Eighty-seven client-owned cats were recruited from 27 veterinary clinics in the Canadian Atlantic provinces. At least 1 year after onychectomy the cats underwent a physical examination, gait analysis, and radiographic evaluation by 1 of 2 authors. There was no significant difference in the frequency of claw regrowth among the 3 methods (P = 0.283). Significantly more cats had bony remnants following guillotine onychectomy (P < 0.001). Bony remnants were significantly associated with claw regrowth (P = 0.001). Cats that underwent laser onychectomy had significantly less post-operative complications (P = 0.023). The long-term outcome was not significantly different among the 3 methods. We conclude that leaving remnants of the third phalanx in situ is associated with an increased frequency of claw regrowth. Laser onychectomy may be preferred to reduce the risk of post-operative complications, bony remnants, and claw regrowth.  相似文献