Asymmetric T lymphocyte division in the initiation of adaptive immune responses   总被引：1，自引：0，他引：1  

Chang JT  Palanivel VR  Kinjyo I  Schambach F  Intlekofer AM  Banerjee A  Longworth SA  Vinup KE  Mrass P  Oliaro J  Killeen N  Orange JS  Russell SM  Weninger W  Reiner SL 《Science (New York, N.Y.)》2007,315(5819):1687-1691

A hallmark of mammalian immunity is the heterogeneity of cell fate that exists among pathogen-experienced lymphocytes. We show that a dividing T lymphocyte initially responding to a microbe exhibits unequal partitioning of proteins that mediate signaling, cell fate specification, and asymmetric cell division. Asymmetric segregation of determinants appears to be coordinated by prolonged interaction between the T cell and its antigen-presenting cell before division. Additionally, the first two daughter T cells displayed phenotypic and functional indicators of being differentially fated toward effector and memory lineages. These results suggest a mechanism by which a single lymphocyte can apportion diverse cell fates necessary for adaptive immunity.  相似文献   

Colchicine-inhibited cilia regeneration: explanation for lack of effect in tris buffer medium   总被引：1，自引：0，他引：1  

L Margulis  S Banerjee  T White 《Science (New York, N.Y.)》1969,164(884):1177-1178

In Stentor coeruleus growth of new, daughter ciliates and experimentaly inducled regeneration of oral membranellar cilia are reversibly inhibited by low, nontoxic concentrations of colchicine. However, if the clulture medium containing colchicine (or Colcemid) is made up in tris(hydroxymethyl)aminomethane buffer, growth of ciliated daughters and regeneration of oral cilia proceed normally. The evidence suggests that the mechanism of this reversal of the effects of colchicine (or Colcemid) is due to a chemical reaction between tris(hydroxymethyl)-aminomethane (or its hydrochloride, or both) and colchicine (or Colcemid), wihich reduces the effective concentration of these mitotic spindle inhibitors reaching the stentors.  相似文献   

Isolated chromaffin cells from adrenal medulla contain primarily monoamine oxidase B   总被引：6，自引：0，他引：6  

M B Youdim  D K Banerjee  H B Pollard 《Science (New York, N.Y.)》1984,224(4649):619-621

Cultured chromaffin cells from bovine adrenal medulla were found to contain primarily the B form of monoamine oxidase. This monoamine oxidase B enzyme was somewhat distinct from B enzymes from other sources, in that noradrenaline was a much poorer substrate than serotonin. Nonetheless, studies with selective inhibitors of the A form (clorgyline) and the B form [(-)-deprenyl] confirmed that chromaffin cell monoamine oxidase was the B form. The observation that chromaffin cell monoamine oxidase has poor affinity for catecholamines is consistent with physiological needs that require the cell to synthesize and store large amounts of catecholamines.  相似文献   

Methyltetrahydrofolic acid mediates N- and O-methylation of biogenic amines   总被引：1，自引：0，他引：1  

S P Banerjee  S H Snyder 《Science (New York, N.Y.)》1973,182(107):74-75

A variety of mammalian and avian tissues N- and O-methylate indoleamines and phenylethylamines, with methyltetrahydrofolic acid as the methyl donor. Because it is considerably more efficient than S-adenosylmethionine, methyltetrahydrofolic acid may be the natural methyl donor in this reaction. With methyltetrahydrofolic acid, serotonin is O-methylated to 5-methoxytryptamine, a novel indoleamine in mammalian brain.  相似文献   

Geophysics. When the compass stopped reversing its poles     

Banerjee SK 《Science (New York, N.Y.)》2001,291(5509):1714-1715

  相似文献   

Drugs, neurotransmitters, and schizophrenia     

Snyder SH  Banerjee SP  Yamamura HI  Greenberg D 《Science (New York, N.Y.)》1974,184(4143):1243-1253

  相似文献   

Genetics of Resistance to Fruit Borer, Heliothis armigera Hübner, in Lycopersicon     

Kalloo    M. K. Banerjee    R. K. Kashyap  A. K. Yadav 《Plant Breeding》1989,102(2):173-175

Inheritance of resistance to the fruit borer, Heliothis armigera Hübner in tomato was studied in TWO interspecific crosses. L. hirsutum f. gLabratum‘B 6013′. a fruit borer-resistant parent, was crossed with two susceptible L. esculentum varieties ‘HS 101’ and. ‘HS 102′. Six generations of two crosses were evaluated for percentage fruit: infestation (on a number basis) and the estimates of the gene effects were derived from the generation mean using a three parameter model A preponderance of additive gene effects was found for this trait. Heritability in the narrow sense was observed to be high indicating the fixable nature of the character.  相似文献   

Histopathology of respiratory organs of certain air-breathing fishes of India     

T. K. Banerjee 《Fish physiology and biochemistry》2007,33(4):441-454

Air-breathing fishes have evolved bimodal respiratory mechanisms for exploitation of water (through gills and highly vascularized skin) as well as atmospheric air (through aerial respiratory organs, ABO). Mucous cells in these respiratory organs of variously stressed fishes exhibit periodic fluctuations in their density and staining properties. The main types of damage in the gills include congestion of blood capillaries (BLCs), periodic lifting and sloughing of respiratory epithelia of the secondary lamellae causing haemorrhage, extensive fusion of secondary lamellae and hyperplasia of the respiratory epithelia due to uncontrolled regeneration leading to asphyxiation, altered excretion, and death of the fish. Haemolysis has also been observed following lead exposure. The damage in the ABO of Heteropneustes fossilis includes sloughing of the epithelial cells, leading to haemorrhage causing decreased red blood corpuscles density and degeneration of the secondary gill lamellae with reduced respiratory area. Subsequent hyperplasia of the respiratory epithelia and fusion of gill lamellae increase the respiratory barrier distance. The BLCs often bulge out and protrude into the lumen, bringing blood nearer to air. The ladder-like pillar cell (PLC)-BLC components of the gill lamellae frequently collapse. Damage to the ABO of Channa striata is less severe. Often haemorrhaging due to bursting of extensively stretched BLCs causes aerial respiratory failure. Chloride cells of the ABOs also show hyperplasia. While the highly mucogenic epidermis of C. striata shows less damage, the epidermis of Clarias batrachus and H. fossilis shows severe wear and tear, sloughing, and haemorrhage. Side-by-side regeneration continues, causing altered histomorphology of the epidermis. The different gland cells also show periodic fluctuations in their density and staining. The dermis also shows severe damage with loosening of their connective tissue fibres. These fibres give stronger reactions for sulfated mucin that not only retain additional water molecules for continuance of skin breathing, but also bind the toxic ambient pollutants.  相似文献   

The significance of microbial biomass sulphur in soil   总被引：2，自引：0，他引：2  

M. R. Banerjee  S. J. Chapman 《Biology and Fertility of Soils》1996,22(1-2):116-125

The soil microbial biomass S fraction of total organic S in soil is considered to be relatively labile and the most active S pool for S turnover in soil. Its significance has been demonstrated in studies of S deficiency in agronomic situations and in those of S pollution from high atmospheric inputs. The utility of the CHCl₃ fumigation-extraction technique for the measurement of microbial S has been proved for a range of soils and conditions. The various methodologies currently available are discussed, including the need for determination of the conversion (K _s) factor. Microbial S values, summarized from the available literature, ranged from 3 to 300 g S g^-1 dry weight soil. They were generally greater in grassland than in arable systems, though the greatest values were obtained in the few examples from forest and peatland soil systems. Microbial S values showed direct relationships with both microbial C and with total soil organic S. Again, there were significant differences between arable and grassland systems. The effect of factors such as organic and inorganic inputs as well as soil physical conditions on microbial S are described. Microbial S turnover rates were estimated from seasonal, ³⁵S-labelling and modelling studies. These rates varied between an approximately annual turnover rate in undisturbed soils up to 80 year^-1 following the addition of readily available substrates. Prospective future research areas are also outlined.  相似文献   

Effects of dicyandiamide, farmyard manure and irrigation on crop yields and ammonia volatilization from an alluvial soil under a rice (Oryza sativa L.)-wheat (Triticum aestivum L.) cropping system     

B. Banerjee  H. Pathak  P. Aggarwal 《Biology and Fertility of Soils》2002,36(3):207-214

Volatilization of NH₃ from soil is a major N-loss mechanism that reduces the efficiency of applied N fertilizers, and causes environmental pollution. Strategies are needed to reduce the loss. The influences of dicyandiamide (DCD), farmyard manure (FYM) and irrigation on NH₃ volatilization from an alluvial soil in rice (Oryza sativa L.)-wheat (Triticum aestivum L.) cropping system was studied using the acid trap method. The loss of NH₃ in the rice-wheat system ranged from 38.6 kg N ha^-1 from the unfertilized soil to 69.0 kg N ha^-1 in the treatment with urea+DCD. Substitution of 50% N provided through urea by FYM reduced NH₃-N volatilization by 10% in rice and wheat as compared to the urea treatment. Application of DCD increased NH₃ volatilization in wheat by 7% but in rice it had no effect. The irrigation level had no effect on NH₃ volatilization in rice but fewer irrigations with fewer splits of N in wheat resulted in higher NH₃ volatilization. Application of DCD and FYM with urea had similar effects on grain yield and N uptake by rice and wheat as that of the urea treatment. The study showed that integrated use of organic manure and chemical fertilizer has the potential to reduce the loss of N due to volatilization and thereby minimize environmental pollution. Nitrification inhibitors, which are reported to be useful in increasing the N-use efficiency by reducing the leaching and denitrification losses of N, however, may increase N loss due to volatilization.  相似文献