The W‐ and Z‐linked EE0.6 sequences used for molecular sexing of captive Japanese crested ibis on Sado Island     

Kaori KASUGA  Maho HIGASHI  Takahisa YAMADA  Toshie SUGIYAMA  Yukio TANIGUCHI  Hiroaki IWAISAKI  Kosuke HOMMA  Yuuichi WAJIKI  Yoshinori KANEKO  Satoshi YAMAGISHI 《Animal Science Journal》2012,83(1):83-87

The Japanese crested ibis Nipponia nippon is a critically threatened bird. Accurate sexing is necessary to perform effective management of captive breeding toward a national project for a tentative release of the Japanese crested ibis on Sado Island. A PCR‐based sexing method targeting a 0.6 kb EcoRI fragment (EE0.6) sequence on W chromosome with AWS03 and USP3 primers has been developed for the Japanese crested ibis. However, the primers were selected from the EE0.6 sequences from bird species other than the Japanese crested ibis. In this study, we determined the W‐ and Z‐linked EE0.6 sequences in the Japanese crested ibis, and clarified Japanese crested ibis sequence mismatch in the binding sites of the primers. Further, we found no polymorphism in the primer binding sites among five founder birds for the Sado captive Japanese crested ibis population. These findings validated the PCR‐based sexing method with the AWS03 and USP3 as accurate molecular sexing methods of captive Japanese crested ibis on the Sado Island. Additionally, we designed a primer set for a novel PCR‐based sexing, based on the EE0.6 sequences obtained in this study. This novel sexing method may be useful for future ecological research following the release of Japanese crested ibis on Sado Island. This is the first report to show the EE0.6 sequences in Japanese crested ibis.  相似文献   

Pilot Study of Bovine Interdigital Cassetteless Computed Radiography     

El-Sayed Ahmed Awad EL-SHAFAEY  Takahiro AOKI  Mitsuo ISHII  Kazutaka YAMADA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2013,75(11):1503-1506

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Reestablishment of transzonal projections and growth of bovine oocytes in vitro     

Mihoko FUSHII  Rie YAMADA  Jibak LEE  Takashi MIYANO 《The Journal of reproduction and development》2021,67(5):300

Transzonal projections (TZPs) that maintain bidirectional communication between oocytes and granulosa cells or cumulus cells are important structures for oocyte growth. However, whether TZPs develop between TZP-free oocytes and granulosa cells, and whether reestablished TZPs support oocyte growth, is unknown. We first examined changes in TZPs after denudation of bovine oocytes collected from early antral follicles (0.5–0.7 mm). Twenty-four hours after denudation, almost all the TZPs disappeared. We also examined the reestablishment of TZPs by coculturing TZP-free denuded oocytes (DOs) with mural granulosa cells (MGCs) collected from early antral follicles. In addition, to confirm if the reestablished TZPs were functional, the reconstructed complexes (DO+MGCs) were subjected to in vitro growth culture and found that the MGCs adhered to TZP-free DOs and TZPs were reestablished. During in vitro growth culture, DO+MGCs developed and formed antrum-like structures. After culture, the number of TZPs in DO+MGCs increased, and the oocytes grew fully and acquired meiotic competence. These results suggest that reestablished TZPs are able to support oocyte growth.  相似文献   

Work-related increases in titer of Campylobacter jejuni antibody among workers at a chicken processing plant in Miyazaki prefecture,Japan, independent of individual ingestion of edible raw chicken meat     

Elisabet TANGKONDA  Meiko KUBO  Satoshi SEKIGUCHI  Taisuke SHINKI  Satomi SASAKI  Kentaro YAMADA  Takako TANIGUCHI  Torrung VETCHAPITAK  Naoaki MISAWA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(8):1306

Workers in poultry abattoirs may be frequently exposed to Campylobacter jejuni, which is a leading cause of bacterial food poisoning in Japan. The present study was conducted to measure the titers of IgG and IgA antibodies against C. jejuni among 104 female workers in a chicken processing plant in Miyazaki prefecture, Japan. Information regarding habitual ingestion of raw chicken meat and potential occupational risk factors was collected using a questionnaire. Acid extracts of four C. jejuni strains representing the genotypes most dominant in Miyazaki were used as antigens. The levels of both immunoglobulins measured by ELISA were not correlated with ingestion of edible raw chicken meat, the amount consumed in one sitting, or its frequency. Although age was correlated with antibody levels, the length of employment was not. Furthermore, the IgG and IgA levels in workers at the evisceration step were significantly higher than those at other locations in the plant. To identify the bacterial proteins recognized by the workers’ IgG and IgA antibodies, Western blotting followed by LC/MS was conducted. Flagellin was identified as the common protein recognized in the sera of workers for whom ELISA demonstrated both the highest and lowest antibody levels. We concluded that the titers of IgG and IgA against C. jejuni in workers at the processing plant had been increased by occupational exposure to Campylobacter, regardless of raw chicken meat ingestion.  相似文献   

Evaluation of the Cranial Cruciate Ligament Repair System? in Surgery for Laryngeal Hemiplegia in Heavy Draft Horses     

Naoki SASAKI  Yoshinori MORITA  Tomoe MORIYAMA  Haruo YAMADA 《Journal of Equine Science》2009,20(2):19-22

The purpose of this study was to evaluate the effect of the canine cranial cruciate ligament repair system on laryngeal hemiplegia in heavy draft horses. Twenty-four heavy draft horses diagnosed with grade 4 laryngeal hemiplegia were allocated to either the prosthetic laryngoplasty (PL) group (n=14) or a canine cranial cruciate ligament repair system (CCCLRS) group (n=10). Right to left angle quotients (RLQs) of abductions of the arytenoid cartilages were endoscopically evaluated before and after surgery. Post-operative RLQs in the CCCLRS group were significantly lower than those of the PL group (P<0.01). The canine cranial cruciate ligament repair system was revealed to be a good surgical procedure for laryngeal hemiplegia in heavy draft horses.  相似文献   

Novel SNP in 5' flanking region of EDG1 associated with marbling in Japanese Black beef cattle     

Takahisa YAMADA  Seiki SASAKI  Shin SUKEGAWA  Takeshi MIYAKE  Tatsuo FUJITA  Hiroyuki KOSE  Mitsuo MORITA  Youichi TAKAHAGI  Hiroshi MURAKAMI  Fumiki MORIMATSU  Yoshiyuki SASAKI 《Animal Science Journal》2009,80(4):486-489

Marbling, defined by the amount and distribution of intramuscular fat, is an economically important trait of beef cattle in Japan. The endothelial differentiation , sphingolipid G-protein-coupled receptor , 1 ( EDG1 ) gene has been considered as a positional functional candidate for the gene responsible for marbling. We have recently reported that 2 single nucleotide polymorphisms (SNPs), c.-312A > G in the 5' untranslated region (UTR) and c.*446G > A in the 3' UTR in EDG1 were associated with marbling in Japanese Black beef cattle, but this was not functional and a causal mutation for marbling. In the present study, we detected 2 novel SNPs, referred to as g.1475435G > A and g.1471620G > T , in the 5' flanking region of the EDG1 between low-marbled and high-marbled steer groups, which were previously shown to have EDG1 expression differences in musculus longissimus muscle. The g.1475435G > A SNP seemed not to segregate in Japanese Black beef cattle. The g.1471620G > T SNP was associated with the predicted breeding value for beef marbling standard number by the analyses using Japanese Black beef cattle population. Based on these findings, we hypothesized that the g.1471620G > T SNP might have an impact on EDG1 expression and also marbling.  相似文献   

Purulent Necrotic Dislocation of the Hip Joint Associated with Umbilical Infection in a Foal     

Fumie MAGATA  Mitsuo ISHII  Eri OIKAWA  Hidefumi FURUOKA  Kazutaka YAMADA  Naoki SASAKI  Syo SHIMIZU  Hisashi INOKUMA 《Journal of Equine Science》2010,21(2):17-20

A 42-day-old heavy draft horse fell into sudden astasia. Significant swelling and heat sensation of the left femoral region were observed. Because of a friction sound in the left hip, we supposed that the hip joint was dislocated or the hip bone was fractured. Computed Tomography (CT) examination showed that the left hip joint was dislocated and the left femoral head was disjunct. We carried out a pathological autopsy, and made a diagnosis of the foal as fracture of the hip bone and femoral head with suppurative umbilical arteritis. Pathologic changes in the umbilical artery and hind leg were completely unilateral, suggesting that left umbilical arteritis spread to the blood circulation, causing arthritis and dislocation of the hip bone.  相似文献   

The T allele at the g.1471620G>T in the EDG1 gene associated with high marbling in Japanese Black cattle is at a low frequency in breeds not selected for marbling     

Naoto WATANABE  Takahisa YAMADA  Sachiyo YOSHIOKA  Masayuki ITOH  Youichi SATOH  Masako FURUTA  Shigeki KOMATSU  Yoshihiko SUMIO  Tatsuo FUJITA  Yoshiyuki SASAKI 《Animal Science Journal》2010,81(1):142-144

Our previous study detected a single nucleotide polymorphism (SNP), g.1471620G > T , in the 5' flanking region of the endothelial differentiation sphingolipid G-protein-coupled receptor 1 ( EDG1 ) gene, which has been considered as a positional functional candidate for the gene responsible for marbling, and showed association of the g.1471620G > T SNP with marbling in Japanese Black beef cattle. In the present study, we investigated the allele frequency distribution of the g.1471620G > T SNP among the 5 cattle breeds, Japanese Black, Japanese Brown, Japanese Short Horn, Holstein, and Brown Swiss breeds. The T allele at the g.1471620G > T SNP associated with high marbling was found at high frequency in Japanese Black breed that has been subjected to a strong selection for high marbling, while the allele was absent or at very low frequencies in the other breeds that have not been strongly selected for high marbling. Based on this finding, we hypothesized that the pressure of the strong selection for high marbling in Japanese Black breed has increased the frequency of the T allele at the g.1471620G > T SNP in the EDG1 .  相似文献   

Protozoan predation of Escherichia coli in hydroponic media of leafy vegetables     

Kiyoshi Sato  Yoshiyuki Taniyama  Ayami Yoshida  Kazuhiko Toyomasu  Noriko Ryuda  Daisuke Ueno 《Soil Science and Plant Nutrition》2013,59(3):234-242

Multiple outbreaks of food poisoning associated with fresh vegetable consumptions have occurred in many countries. Numerous reports have described human pathogenic bacteria, such as Escherichia coli O157:H7 and Salmonella spp., that can internalize into fresh vegetables via root or leaf surfaces. While attempting to obtain the threshold concentration of internalization of E. coli inoculated into hydroponic medium during vegetable cultivation, we observed a rapid decrease in E. coli numbers. In the present study, we determined that the rapid decline in E. coli was not due to a physiological change into a viable but non-culturable (VNC) state. The population crash was instead caused by true bacterial death, as the rapid descent was also confirmed by micro-colony fluorescence in situ hybridization, a culture-independent method that can detect VNC cells. We next monitored the number of E. coli inoculated into intact or filter-sterilized hydroponic medium after cultivation of various types of plants. We found that the number of E. coli in intact hydroponic medium decreased markedly, whereas the level in filter-sterilized hydroponic medium was completely unchanged. This result suggests that biotic factors were present that could be eliminated by filtering. Robust predation of E. coli by protozoa (ciliates and flagellates) was observed using fluorescently labeled bacteria incorporated into the hydroponic medium. Finally, morphological identification of flagellates by scanning electron microscopy revealed the presence of a species of Stramenopiles. These findings suggest the importance of protozoa as bacterial feeders in hydroponic systems and hence the use of these organisms as potential control agents of human pathogenic bacteria.  相似文献   

Causes of low seed set in white clover (Trifolium repens L.)   总被引：1，自引：0，他引：1  

S. V. PASUMARTY  T. MATSUMURA  S. HIGUCHI  T. YAMADA 《Grass and Forage Science》1993,48(1):79-83

A field experiment was conducted to clarify the factors responsible for poor seed set in four white clover ( Trifolium repens L.) cultivars. Although the mean number of ovules per floret was 5·18–5·29, the average number of seeds per floret was found to be only 1·23-1·82. A stainclearing technique was used to examine the cytoplasmic state of the embryo sac in intact, unfertilized, mature ovules and it was found that 28–33% of ovules per carpel were sterile. The abortion rate of fertilized ovules and immature seeds (7 days after pollination) ranged between 32 and 34%. Beyond this period, it was observed that only a very small proportion of the total seeds were lost. The observation of high pollen fertility and a high pollen load strongly suggests that poor pollination does not account for the reduced number of seeds per floret. A 'nutrient competition' hypothesis was proposed for high ovule sterility and post-fertilization abortion of developing seeds.  相似文献