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Responses of swamp bay,Persea palustris,and avocado,Persea americana,to various concentrations of the laurel wilt pathogen,Raffaelea lauricola
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M. A. Hughes S. A. Inch R. C. Ploetz H. L. Er A. H. C. van Bruggen J. A. Smith 《Forest Pathology》2015,45(2):111-119
Laurel wilt, caused by the fungus Raffaelea lauricola and transmitted by the exotic ambrosia beetle Xyleborus glabratus, has killed members of the Lauraceae plant family throughout the southeast United States. A series of experiments were conducted to examine the effects of inoculum concentration on the development of laurel wilt in swamp bay, Persea palustris, and avocado, Persea americana. In each experiment, host plants were inoculated with aqueous suspensions of 102, 103, 104 or 105 conidia of R. lauricola, and plants were rated periodically for external symptom development (wilting and foliar dieback). At the end of experiments, plants were rated for internal symptoms of the disease (discoloration of sapwood) and assayed for R. lauricola on a semi‐selective medium. Symptom severity in swamp bay was significantly lower for the 102 treatment than at higher (103–105) concentrations, whereas 102 and 103 conidia caused less disease than 104 and 105 conidia in avocado. At the lowest inoculum concentration, 67% of the swamp bay plants and 20% of the avocados died by the time the respective experiments were terminated. The pathogen was recovered from a high proportion of the symptomatic sapwood of swamp bay (100%) and avocado (94%), and sapwood discoloration and recovery of R. lauricola from inoculated stems of swamp bay were highly correlated with recovery of the pathogen and symptom development in roots. Clearly, swamp bay and avocado are very sensitive to R. lauricola. The ability of only 100 conidia of this pathogen to kill these hosts suggests that few individuals of X. glabratus or other ambrosia beetles that carry low levels of the pathogen would be sufficient to transmit conidia that infect and lead to disease development. The results are also relevant to the development of disease‐tolerant host selections, as they indicate levels of the pathogen appropriate for use in screening plants for disease resistance. 相似文献
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Orhan Corum Duygu Durna Corum Orkun Atik Feray Altan Ayse Er Kamil Uney 《Journal of veterinary pharmacology and therapeutics》2019,42(6):654-659
The pharmacokinetics and bioavailability of levamisole were determined in red‐eared slider turtles after single intravenous (IV), intramuscular (IM), and subcutaneous (SC) administration. Nine turtles received levamisole (10 mg/kg) by each route in a three‐way crossover design with a washout period of 30 days. Blood samples were collected at time 0 (pretreatment), and at 0.25, 0.5, 1, 1.5, 3, 6, 9, 12, 18, 24, 36, and 48 hr after drug administration. Plasma levamisole concentrations were determined by a high‐performance liquid chromatography assay. Data were analyzed by noncompartmental methods. The mean elimination half‐life was 5.00, 7.88, and 9.43 hr for IV, IM, and SC routes, respectively. The total clearance and volume of distribution at steady state for the IV route were 0.14 L hr?1 kg?1 and 0.81 L/kg, respectively. For the IM and SC routes, the peak plasma concentration was 9.63 and 10.51 μg/ml, respectively, with 0.5 hr of Tmax. The bioavailability was 93.03 and 115.25% for the IM and SC routes, respectively. The IM and SC route of levamisole, which showed the high bioavailability and long t1/2?z, can be recommended as an effective way for treating nematodes in turtles. 相似文献
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This experiment was carried out to determine the percentage of internal connection between rootstock and scion in graft union with nondestructive and noninvasive MRI (Magnetic Resonance Imaging) method and to follow performance status according to internal connection ratios of the grafted rooted vines after planting to vineyard field. Research was established in a factorial randomized block design and carried out with cvs. Merlot and Syrah grafted onto 110?R rootstock and 4 different internal (MRI) connection levels in 3 replications. The percentages of grafted rooted vine internal determination by MRI by four sides (13.75%) in graft union were found to be very low than others at the pre-planting stage. An increase in the internal connection ratio in the majority of the grafted rooted vines was determined after the vegetative growth phase. Therefore, the internal connection ratios of the graft union of rooted vines of cvs. Merlot and Syrah varieties showed a tendency to increase during the second year of development. Again in both cultivars, loss rate of grafted rooted vines showed a decreasing tendency depending on increase of internal connection ratio of graft union. As a result, in case of decrease of MRI costs, it is thought that grafted rooted vine producers may have the opportunity to supply better quality seedlings to vine growers using MRI techniques. 相似文献
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高效液相色谱法检测泰乐菌素在肉鸡组织中的残留 总被引:4,自引:0,他引:4
将肉鸡的肌肉、肝脏、肾脏和脂肪组织制成匀浆,用乙腈、石油醚和二氯甲烷萃取、净化后,用乙腈定容。以乙腈- 甲醇- 0.005 m ol/ L磷酸二氢铵溶液(60∶30∶10)为流动相,用高效液相色谱检测。本法对溶解于乙腈中的泰乐菌素的最低检测限为 0.05 m g/ L,在肉鸡各种组织中的最低检测限为 0.10 m g/ L。标准曲线的线性范围为 0.10~6.40 m g/ L。泰乐菌素质量浓度为 0.10、0.80、6.40 m g/ L 时的批间变异系数分别为(2.3±0.2)% 、(1.9±1.3)% 、(2.6±0.9)% 。0.20、0.40、0.80 m g/ L 的泰乐菌素在以上 4 种组织的回收率均符合残留检测的要求。结果表明,本法是一种灵敏、精密、可靠的检测肉鸡组织中泰乐菌素残留的分析方法。 相似文献
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目前,准确估算河流泥沙通量是实施河流泥沙总量控制的前提。以新疆北疆河流1980年的含沙量资料为基础,基于负荷历时曲线方法计算泥沙通量,分析泥沙通量的年际变化及其影响因素,探讨基于负荷历时曲线通量估算的不确定性、适用性。得出基于负荷历时曲线的通量估算方法能较准确估算新疆河流泥沙通量,可为新疆河流泥沙通量的研究提供新思路,为泥沙防治工作提供理论依据,具有一定的学术意义和应用价值。 相似文献
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Serhat Türkmen Orhan Tufan Eroldoğan Hatice Asuman Yılmaz Abdüllatif Ölçülü Gül Ayten Kiriş Inan Zeynep Erçen Nazmi Tekelioğlu 《Aquaculture Research》2012,43(11):1643-1650
The compensatory growth response of the European sea bass (Dicentrarchus labrax) that faced cycling starvation and restricted ration was assessed. Juveniles (10.5 g) were stocked into 15 tanks at a density of 25 fish per tank. Five different feeding regimes were tested on triplicate groups of fish: CSatiation: control fed for 60 days without deprivation, CRestricted: 25% restricted feeding, S‐R: 1 day starvation then 4 days CRestricted feeding, R‐F: 1 day CRestricted feeding then 4 days CSatiation feeding, and, finally, S‐F: 1 day starvation then 4 days CSatiation feeding. The specific growth rate of fish in the CSatiation (2.5 ± 0.06% day?1), S‐F (2.5 ± 0.11% day?1) and R‐F (2.4 ± 0.18% day?1) were significantly higher than that of CRestricted (2.2 ± 0.05% day?1) or S‐R (2.0 ± 0.01% day?1). Fish in S‐F group were able to achieve catching up with the CSatiation. There was no significant difference in feed conversion rates but R‐F and S‐F consumed approximately 34% more feed than CSatiation following the first re‐feeding day. Although, the highest lipid content was observed in CSatiation (14.4%), S‐R (33.3% dry matter) had the highest water content compared with the CSatiation and CRestricted (37.8% and 36.9% dry matter respectively). In conclusion, it may be concluded that sea bass has rapid response to cycling starvation/re‐feeding and that a 25% restricted feeding ratio is insufficient to invoke a compensatory growth response in sea bass. 相似文献