Measurement of Total Dietary Fiber Using AOAC Method 2009.01 (AACC International Approved Method 32‐45.01): Evaluation and Updates     

Barry V. McCleary  Naomi Sloane  Anna Draga  Ida Lazewska 《Cereal Chemistry》2013,90(4):396-414

The Codex Committee on Methods of Analysis and Sampling recently recommended 14 methods for measurement of dietary fiber, eight of these being type I methods. Of these type I methods, AACC International Approved Method 32‐45.01 (AOAC method 2009.01) is the only procedure that measures all of the dietary fiber components as defined by Codex Alimentarius. Other methods such as the Prosky method (AACCI Approved Method 32‐05.01) give similar analytical data for the high‐molecular‐weight dietary fiber contents of food and vegetable products low in resistant starch. In the current work, AACCI Approved Method 32‐45.01 has been modified to allow accurate measurement of samples high in particular fructooligosaccharides: for example, fructotriose, which, in the HPLC system used, chromatographs at the same point as disaccharides, meaning that it is currently not included in the measurement. Incubation of the resistant oligosaccharides fraction with sucrase/β‐galactosidase removes disaccharides that interfere with the quantitation of this fraction. The dietary fiber value for resistant starch type 4 (RS₄), varies significantly with different analytical methods, with much lower values being obtained with AACCI Approved Method 32‐45.01 than with 32‐05.01. This difference results from the greater susceptibility of RS₄ to hydrolysis by pancreatic α‐amylase than by bacterial α‐amylase, and also a greater susceptibility to hydrolysis at lower temperatures. On hydrolysis of samples high in starch in the assay format of AACCI Approved Method 32‐45.01 (AOAC method 2009.01), resistant maltodextrins are produced. The major component is a heptasaccharide that is highly resistant to hydrolysis by most of the starch‐degrading enzymes studied. However, it is hydrolyzed by the maltase/ amyloglucosidase/isomaltase enzyme complex present in the brush border lining of the small intestine. As a consequence, AOAC methods 2009.01 and 2011.25 (AACCI Approved Methods 32‐45.01 and 32‐50.01, respectively) must be updated to include an additional incubation with amyloglucosidase to remove these oligosaccharides.  相似文献   

Effect of method of irrigation on the total glycoalkaloid and nitrate-nitrogen content of Rosa potatoes     

Barry Gosselin  Nell I. Mondy  W. D. Evans 《American Journal of Potato Research》1988,65(2):99-103

The effect of two irrigation methods, microjet mist and impact sprinkler, on the total glycoalkaloid (TGA) and nitrate-nitrogen (NO_3?N) content ofSolanum tuberosum L. cv. Rosa was investigated. Potatoes irrigated by microjet mist were significantly higher in TGA and lower in NO_3?N content than control potatoes receiving no irrigation. The impact sprinkler method produced potatoes significantly higher in NO_3?N than controls. The TGA content of potatoes irrigated by the sprinkler method was not significantly different from controls. Mist-irrigated potatoes were higher in TGA and lower in NO_3?N content than sprinkler-irrigated potatoes.  相似文献   

Rapid phase adjustment of melatonin and core body temperature rhythms following a 6-h advance of the light/dark cycle in the horse     

Barbara A Murphy  Jeffrey A Elliott  Dawn R Sessions  Mandi M Vick  Erin L Kennedy  Barry P Fitzgerald 《Journal of circadian rhythms》2007,5(1):5-9

Background  

Rapid displacement across multiple time zones results in a conflict between the new cycle of light and dark and the previously entrained program of the internal circadian clock, a phenomenon known as jet lag. In humans, jet lag is often characterized by malaise, appetite loss, fatigue, disturbed sleep and performance deficit, the consequences of which are of particular concern to athletes hoping to perform optimally at an international destination. As a species renowned for its capacity for athletic performance, the consequences of jet lag are also relevant for the horse. However, the duration and severity of jet lag related circadian disruption is presently unknown in this species. We investigated the rates of re-entrainment of serum melatonin and core body temperature (BT) rhythms following an abrupt 6-h phase advance of the LD cycle in the horse.  相似文献   

Measurement of cereal α-amylase: A new assay procedure     

Barry V. McCleary  Helen Sheehan 《Journal of Cereal Science》1987,6(3)

  相似文献   

Incorporation of urea-derived 13C into microbial communities in four different agriculture soils     

Juan Wang  Barry Thornton  Huaiying Yao 《Biology and Fertility of Soils》2014,50(4):603-612

The objective of this study was to investigate changes in the composition of the soil microbial community brought about by urea application and differences in the incorporation of urea-derived C into the soil phospholipid fatty acid (PLFA) pool at differing soil pH. We selected four soils which ranged in pH from 3.9 to 7.8. ¹³C-labeled urea was applied at two concentrations 100 and 200 mg N kg^?1 which represents commonly used and high levels of application. Significant hydrolysis of applied urea occurred within 2 h; less than 2 % of urea-C was retained in the soil with one exception, the fluvo-aquic soil at pH 7.8 amended with 200 mg kg^?1 urea-N 3 days after urea application. According to principal component analysis (PCA), the effect of urea and incubation time on microbial community composition was far weaker than differences between the four soils due to their large differences in basic properties; the scores of PC2 were significantly correlated with pH values. The incorporation of ¹³C-urea to PLFAs increased with soil pH; this may be related to increases in the speciation of inorganic C into bicarbonate.¹³C label was primarily incorporated into 16:1ω5c, 16:0, and cy19:0 in red soil, pH 3.9; and into 16:1ω7c, 16:0, and 16:1ω5c in fluvo-aquic soil, pH 7.8. A wider range of PLFAs became labeled in the two paddy soils at pH 5.2 and 6.7. This suggests that the profile of PLFAs labeled from the application of ¹³C-urea may be affected by redox potential.  相似文献   

Norman Keith Matheson     

Barry V. McCleary  Anthony Blakeney  Les Copeland  Robert Caldwell 《Journal of Cereal Science》2008,48(3):563-564

  相似文献   

Attraction and antennal response of the common wasp,Vespula vulgaris (L.), to selected synthetic chemicals in New Zealand beech forests     

Ashraf M El‐Sayed  Lee‐Anne Manning  C Rikard Unelius  Kye Chung Park  Lloyd D Stringer  Nicola White  Barry Bunn  Andrew Twidle  David M Suckling 《Pest management science》2009,65(9):975-981

BACKGROUND: The common wasp, Vespula vulgaris (L.), and the German wasp, Vespula germanica (F.), are significant problems in New Zealand beech forests (Nothofagus spp.), adversely affecting native birds and invertebrate biodiversity. This work was undertaken to develop synthetic attractants for these species to enable more efficient monitoring and management. RESULTS: Seven known wasp attractants (acetic acid, butyl butyrate, isobutanol, heptyl butyrate, octyl butyrate and 2,4‐hexadienyl butyrate) were field tested, and only heptyl butyrate and octyl butyrate attracted significantly higher numbers of wasps than a non‐baited trap. Accordingly, a series of straight‐chain esters from methyl to decyl butyrate were prepared and field tested for attraction of social wasps. Peak biological activity occurred with hexyl butyrate, heptyl butyrate, octyl butyrate and nonyl butyrate. Polyethylene bags emitting approximately 18.4–22.6 mg day^?1 of heptyl butyrate were more attractive than polyethylene bags emitting approximately 14.7–16.8 mg day^?1 of heptyl butyrate in the field. Electroantennogram (EAG) studies indicated that queens and workers of V. vulgaris had olfactory receptor neurons responding to various aliphatic butyrates. CONCLUSION: These results are the first to be reported on the EAG response and the attraction of social wasps to synthetic chemicals in New Zealand beech forests and will enable monitoring of social wasp activity in beech forests. Copyright © 2009 Society of Chemical Industry  相似文献   

The rapid and accurate determination of germ tube emergence site by Blumeria graminis conidia     

Alison J. Wright  Tim L. W. Carver  Barry J. Thomas  Nick I. D. Fenwick  Hitoshi Kunoh  Ralph L. Nicholson 《Physiological and Molecular Plant Pathology》2000,57(6):281

The development of appressoria by germinating Blumeria graminis conidia depends on its germ tubes making contact with the host surface. Low angle, low temperature scanning electron microscopy showed that 80% or more of first-formed germ tubes of f. spp. hordei, tritici and avenae conidia emerged from close to the host leaf surface, and so made contact with it allowing them to become functional primary germ tubes. Light microscopy of f. sp.hordei confirmed this result and, in addition, showed that germ tubes frequently emerged close to, and contacted, various hydrophobic and hydrophilic artificial substrata. Geometric models of conidium-substratum interfaces were developed and a “surface point method” was derived to predict the frequency with which contact would result if germ tube emergence was a random phenomenon. However, observed contact frequencies were far higher (c. three to eight times) than predicted. Thus, the germ tube emergence site was determined as a response to substratum contact. In part, this appeared to be a non-specific response. Nevertheless, germ tube contact frequencies were greater on the curved surface of leaf epidermal cells than on planar surface, suggesting that specific recognition of leaf surface characteristics may promote directional emergence. The area of contact required to stimulate directional germ tube emergence was very small: contact with a microneedle tip or with a spiders’ suspension thread was sufficient for many conidia. Similarly, on leaves, the only contact is between the tips of a limited number of conidial wall projections and the edges of epicuticular leaf wax plates. Micromanipulation to roll conidia so that their original site of contact with a leaf was rotated away from it, led to the majority of first-formed germ tubes growing away from the leaf, i.e. emerging close to the site of original contact. The experiments indicated that the site of germ tube emergence is determined within 1 min of deposition. This implicated the release of conidial extracellular materials in recognition of the conidium-leaf surface contact site.  相似文献   

Suberitamides A–C,Aryl Alkaloids from a Pseudosuberites sp. Marine Sponge that Inhibit Cbl-b Ubiquitin Ligase Activity     

Chang-Kwon Kim  Dongdong Wang  Brice A. P. Wilson  Josep Saurí  Donna Voeller  Stanley Lipkowitz  Barry R. O’Keefe  Kirk R. Gustafson 《Marine drugs》2020,18(11)

  相似文献   

An aggregation index (AI) to quantify spatial patterns of landscapes   总被引：43，自引：0，他引：43  

He  Hong S.  DeZonia  Barry E.  Mladenoff  David J. 《Landscape Ecology》2000,15(7):591-601

There is often need to measure aggregation levels of spatial patterns within a single map class in landscape ecological studies. The contagion index (CI), shape index (SI), and probability of adjacency of the same class (Qi), all have certain limits when measuring aggregation of spatial patterns. We have developed an aggregation index (AI) that is class specific and independent of landscape composition. AI assumes that a class with the highest level of aggregation (AI =1) is comprised of pixels sharing the most possible edges. A class whose pixels share no edges (completely disaggregated) has the lowest level of aggregation (AI =0). AI is similar to SI and Qi, but it calculates aggregation more precisely than the latter two. We have evaluated the performance of AI under varied levels of (1) aggregation, (2) number of patches, (3) spatial resolutions, and (4) real species distribution maps at various spatial scales. AI was able to produce reasonable results under all these circumstances. Since it is class specific, it is more precise than CI, which measures overall landscape aggregation. Thus, AI provides a quantitative basis to correlate the spatial pattern of a class with a specific process. Since AI is a ratio variable, map units do not affect the calculation. It can be compared between classes from the same or different landscapes, or even the same classes from the same landscape under different resolutions.  相似文献