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11.
Rita M. Hanel DVM DACVIM DACVECC Lee Palmer DVM MS DACVECC NREMT‐T WEMT CCRP Janice Baker DVM DACVPM Jo‐Anne Brenner BA EMT‐I EMT‐T David Dorman DVM PhD DABVT John C. Gicking DVM DACVECC Brian Gilger DVM MS DACVO DABT Cynthia M. Otto DVM PhD DACVECC DACVSMR Elizabeth Rozanski DVM DACVECC DACVIM Brian Trumpatori DVM 《Journal of Veterinary Emergency and Critical Care》2016,26(2):166-233
12.
我国西部地区水电开发问题探讨 总被引:1,自引:0,他引:1
水能资源是我国西部地区的优势资源,由于历史和政策的原因,开发利用程度相对较低,资源优势没有形成产业优势。在分析西部水能资源分布特点和开发现状的基础上,结合西部大开发和西电东送战略,提出目前水电产业存在的市场、政策和资金方面问题,探讨了加快西部水电开发的对策和建议。 相似文献
13.
Homer BL Li C Berry KH Denslow ND Jacobson ER Sawyer RH Williams JE 《American journal of veterinary research》2001,62(1):104-110
OBJECTIVES: To characterize protein composition of shell scute of desert tortoises and to determine whether detectable differences could be used to identify healthy tortoises from tortoises with certain illnesses. ANIMALS: 20 desert tortoises. PROCEDURES: Complete postmortem examinations were performed on all tortoises. Plastron scute proteins were solubilized, scute proteins were separated by use of sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), and proteins were analyzed, using densitometry. Two-dimensional immobilized pH gradient-PAGE (2D IPG-PAGE) and immunoblot analysis, using polyclonal antisera to chicken-feather beta keratin and to alligator-scale beta keratin, were conducted on representative samples. The 14-kd proteins were analyzed for amino acid composition. RESULTS: The SDS-PAGE and densitometry revealed 7 distinct bands, each with a mean relative protein concentration of > 1 %, ranging from 8 to 47 kd, and a major protein component of approximately 14 kd that constituted up to 75% of the scute protein. The 2D IPG-PAGE revealed additional distinct 62- and 68-kd protein bands. On immunoblot analysis, the 14-, 32-, and 45-kd proteins reacted with both antisera. The 14-kd proteins had an amino acid composition similar to that of chicken beta keratins. There was a substantial difference in the percentage of the major 14-kd proteins from scute of ill tortoises with normal appearing shells, compared with 14-kd proteins of healthy tortoises. CONCLUSIONS AND CLINICAL RELEVANCE: The major protein components of shell scute of desert tortoises have amino acid composition and antigenic features of beta keratins. Scute protein composition may be altered in tortoises with certain systemic illnesses. 相似文献
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A few soil-borne plant pathogens have been controlled successfully by commercial formulations of biocontrol agents, but many attempts to develop biocontrol inoculants, although promising under experimental conditions, have met with difficulties in practice. The reasons for this are discussed in this review, which outlines some of the major findings on the behaviour of microbial inoculants in soil. It is emphasized that biocontrol also occurs naturally in current agricultural practice and can be exploited purposely, but it is vulnerable to disruption by agrochemicals or mismanagement. The future of biocontrol of soilborne plant pathogens probably lies in integrated (biorational) control systems that combine the use of commercial inoculants, where appropriate, with management practices that maintain and enhance the natural biocontrol systems. 相似文献
16.
Hawkins EC Berry CR 《Journal of the American Veterinary Medical Association》1999,215(11):1635-9, 1620
A technique that did not require use of a bronchoscope for bronchoalveolar lavage (BAL) in dogs was developed. An inexpensive, readily available 16-F Levin-type stomach tube was modified for the procedure. The technique was effective for collecting BAL fluid in 9 dogs that ranged from 9.3 to 26.2 kg (20.5 to 57.6 lb). Recovered fluid was consistent with fluid collected bronchoscopically. Mean recovery volume was 84/125 ml (67%), mean WBC counts were high (> 300 cells/microl), and > 70% of cells were macrophages. Complications from use of the technique were not detected on the basis of pulse oximetry, thoracic radiography, and clinical observation. This effective, simple, and safe technique for BAL can be readily performed in clinical settings that do not have bronchoscopic capabilities. It also provides a less costly alternative than bronchoscopic BAL. 相似文献
17.
Berry SD Weber Nielsen MS Sejrsen K Pearson RE Boyle PL Akers RM 《Domestic animal endocrinology》2003,25(3):245-253
The objectives of the experiment were (1) to determine whether MAC-T cells would accurately mimic the previously observed proliferative responses of primary mammary epithelial cells (MEC) to mammary tissue extracts from high and low-fed heifers and (2) to determine whether mammary tissue extracts from ovariectomized (OVX) heifers would have lower mitogenic activity than intact controls. Addition of mammary tissue extracts to cell culture media of MAC-T cells plated on plastic or collagen-coated plastic to a range of concentrations between 1 and 8% resulted in dose-dependent increases in cell proliferation. Furthermore, mammary tissue extracts from low-fed prepubertal heifers aged 9 months, stimulated significantly more proliferation of MAC-T cells, as measured by 3H-thymidine incorporation into DNA than mammary tissue extracts from high-fed heifers (40.6 cpm x 10(3) per well versus 21.9+/-1.8 cpm x 10(3) per well). These observations suggested that MAC-T cells would be a suitable alternative to primary MECs for measuring the mitogenic activity of mammary tissue extracts. Conversely, no difference was observed in the mitogenic activity of mammary tissue extracts from OVX or control heifers. Possibly, MAC-T cells provide a good model for nutrition- but not ovarian-induced changes in mammary growth. Alternatively, that reduction of in vivo mammary development following OVX did not result in reduced mitogenic activity of the mammary tissue extracts emphasizes that heifer mammary development is the result of complex interactions between local growth factors and systemic hormones. 相似文献
18.
Hawkins EC Basseches J Berry CR Stebbins ME Ferris KK 《Journal of the American Veterinary Medical Association》2003,223(11):1628-1635
OBJECTIVE: To determine demographic, clinical, and radiographic features of bronchiectasis in dogs. DESIGN: Retrospective study. ANIMALS: 289 dogs identified through the Veterinary Medical Database (VMDB) and 27 dogs examined at the North Carolina State University Veterinary Teaching Hospital. PROCEDURE: Demographic characteristics of dogs identified through the VMDB were compared with characteristics of the entire population of dogs entered in the VMDB. Medical records of dogs examined at the teaching hospital were reviewed; the diagnosis was confirmed through review of thoracic radiographs. RESULTS: Analysis of data from the VMDB indicated that American Cocker Spaniels, West Highland White Terriers, Miniature Poodles, Siberian Huskies, English Springer Spaniels, and dogs > 10 years old had an increased risk of bronchiectasis. Among dogs examined at the teaching hospital, coughing was the most common clinical sign. There was evidence for excessive airway mucus but not hemorrhage. A variety of bacterial organisms were isolated from tracheal wash and bronchoalveolar lavage samples. On thoracic radiographs, cylindrical bronchiectasis, generalized disease, and right cranial lung lobe involvement were most common. Seven of 14 dogs for which follow-up radiographs were available did not have any progression of radiographic lesions. Median duration of clinical signs prior to diagnosis of bronchiectasis was 9 months (range, 1 day to 10 years). Median survival time was 16 months (range, 2 days to 72 months). CONCLUSIONS AND CLINICAL RELEVANCE: Results suggest that despite substantial clinical abnormalities, dogs with bronchiectasis may survive for years. Certain purebred dogs and older dogs may have an increased risk of developing bronchiectasis. 相似文献
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Cohen ND Berry SM Peloso JG Mundy GD Howard IC 《Journal of the American Veterinary Medical Association》2000,216(8):1273-1278
OBJECTIVE: To determine the association between high-speed exercise and risk of injury while racing among Thoroughbreds in Kentucky. DESIGN: Matched case-control study. ANIMALS: 206 Thoroughbreds that sustained a musculoskeletal injury while racing and 412 Thoroughbreds that were not injured during the same races. PROCEDURE: Data regarding official timed workouts and races and the Beyer's numbers for the 3 races before the race during which injury occurred were extracted from past performance charts and compared between injured horses and control horses. RESULTS: For injured horses, cumulative distance of high-speed exercise during the 1- and 2-month periods prior to the race in which injury occurred was significantly less than that of control horses; for either period, a difference of 10 furlongs was associated with approximately 2-fold greater risk of injury. Beyer's numbers were significantly higher for injured horses than for control horses. These effects remained significant after adjusting for age and results of prerace physical inspection. CONCLUSIONS AND CLINICAL RELEVANCE: In Kentucky, injured horses had significantly less cumulative high-speed exercise than did control horses during the 1- and 2-month periods prior to the race in which injury occurred. These results differ from those observed in California. The association of injury with cumulative high-speed exercise appears to vary among regions in the United States. 相似文献