Night inshore migration and capture of adult fish by shore seining     

J. KUBE&#;KA 《Aquaculture Research》1993,24(5):685-689

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Determination of arsenic and selenium in whole fish by continuous-flow hydride generation atomic absorption spectrophotometry     

W G Brumbaugh  M J Walther 《Journal of the Association of Official Analytical Chemists》1989,72(3):484-486

A combined wet chemical and dry ash digestion and use of a continuous-flow hydride generator coupled with a flame-heated quartz cell enabled the simple, precise, and highly automated atomic absorption determination of arsenic and selenium in tissues of whole fish. Percent relative standard deviation averaged 4% for each element; method detection limits (micrograms/g dry wt) were about 0.06 for arsenic and 0.04 for selenium. Digestion of samples proceeded with little operator attention and without perchloric acid. Analysis for arsenic as As(V) simplified sample preparation but care had to be exercised to avoid interferences from high concentrations of selenium.  相似文献   

Knowledge and attitudes of Australian livestock producers concerning biosecurity practices     

CC Paquette  KA Schemann  MP Ward 《Australian veterinary journal》2020,98(11):533-545

Biosecurity risks are a major threat to the profitability of the industry as well as impacting human and animal health. Livestock producers play a crucial role in biosecurity as the first to notice changes in the health or productivity of their stock and are generally responsible for implementing protective measures. However, uptake of biosecurity measures by producers is variable. We critically appraised the current literature regarding biosecurity practices in Australian livestock industries and highlight aspects that are well understood as well as those where further research or information is needed. Findings from 12 cross-sectional studies suggest that Australian producers' knowledge of biosecurity methods and importance might have a positive influence on their willingness to implement or incorporate biosecurity practices. There is moderate evidence supportive of biosecurity being well understood by livestock producers across Australia. Barriers to producers using biosecurity practices included lack of information or communication from agricultural, veterinary or government organisations. It was found that larger stock numbers were positively correlated with biosecurity implementation and that producers used veterinarians, government and industry agencies as resources for trusted information.  相似文献   

Stabilized Liquid Membrane Device (SLMD) for the Passive, Integrative Sampling of Labile Metals in Water     

W. G. Brumbaugh  J. D. Petty  J. N. Huckins  S. E. Manahan 《Water, air, and soil pollution》2002,133(1-4):109-119

A stabilized liquid membrane device (SLMD) is described forpotential use as an in situ, passive, integrative samplerfor cadmium (Cd), cobalt (Co), copper (Cu), nickel (Ni), lead(Pb), and zinc (Zn) in natural waters. The SLMD (patent pending)consists of a 2.5-cm-wide by 15-cm-long strip of low-density polyethylene (LDPE) layflat tubing containing 1 mL of an equalmixture (v/v) of oleic acid (cis-9-octadecenoic acid) and EMO-8Q (7-[4-ethyl-1-methyloctyl]-8-quinolinol). The reagentmixture continuously diffuses to the exterior surface of the LDPE membrane, and provides for sequestration of several divalent metals for up to several weeks. Depending on samplerconfiguration, concentration factors of several thousand canbe realized for these metal ions after just a few days. In addition to in situ deployment, the SLMD may be useful for laboratory determination of labile metal species in grab samples. Methods for minimizing the effects of water flow onthe sampling rate are currently under investigation.  相似文献   

Importation of horses for special events     

KA DOYLE 《Australian veterinary journal》1994,71(3):93-93

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Cost-benefit evaluation of artificial insemination for genetic improvement of wool-producing sheep   总被引：1，自引：0，他引：1  

KA ABBOTT 《Australian veterinary journal》1994,71(11):353-360

SUMMARY The financial costs and benefits associated with the use of artificial insemination (AI) in commercial flocks are evaluated. Benefits are calculated in terms of net present values after summing the discounted value of benefits over 20 years. Two breeding strategies are evaluated. With the first, AI is used to produce flock ewes and wethers. The method is unlikely to be profitable unless high breeding value rams are available for AI programs with fresh semen. With the second, AI is used to produce home-bred rams, which in turn sire flock ewes and wethers. This approach is more likely to be profitable. The cost of AI per lamb weaned from laparoscopic AI programs is about $100. Benefits exceed this cost for rams of very high merit when wool prices are moderate or higher. Flock structure has a significant effect on the benefits. Flocks with low wether retention rates have benefits half that of flocks that retain most wethers to 6 years of age. AI with purchased semen also provides benefits to risk management for owners of commercial flocks who wish to breed their own replacement flock rams.  相似文献   

Dermatographism in a horse     

J L Cornick  G W Brumbaugh 《The Cornell veterinarian》1989,79(1):109-116

An eight-year-old Thoroughbred gelding with dermatographic urticaria is presented. Forelimb edema and generalized urticaria were the presenting clinical signs. All hematologic and serum biochemical values were normal. Immunologic and histologic evaluation failed to reveal specific abnormalities. During hospitalization, an urticarial response to mechanical pressure (dermatographism) was identified. Clinical responses to corticosteroid and antihistaminic (H1) therapy were equivocal. The condition resolved in five weeks and an etiology was not discovered. A discussion of the pathogenesis, diagnosis and treatment of dermatographic urticaria in humans is included with the report.  相似文献   

Perioperative antimicrobial considerations for gastrointestinal surgery of cattle     

G W Brumbaugh 《Veterinary Clinics of North America: Food Animal Practice》1990,6(2):307-333

Perioperative use of antimicrobial drugs constitutes extra-label use of those drugs. Principles of therapeutic and prophylactic use of antimicrobial drugs can guide decisions about their perioperative use. Regimens currently suggested are based on those principles, theoretical information, pharmacokinetic and pharmacodynamic data, extrapolation from studies with other species, empirical data, personal bias, and experience. Controlled clinical investigations are necessary to provide objective data from which specific recommendations can be derived.  相似文献   

Application of high-resolution melt curve analysis for classification of infectious bronchitis viruses in field specimens     

KA Hewson  GF Browning  JM Devlin  J Ignjatovic  AH Noormohammadi 《Australian veterinary journal》2010,88(10):408-413

Objective A real-time polymerase chain reaction (PCR)/high-resolution melt (HRM) curve analysis protocol was developed in our laboratory to differentiate infectious bronchitis (IB) virus reference strains. In the current study, this method was used to detect and classify IB viruses in field submissions. Procedure Over an 11-month period samples from 40 cases of suspected IB virus were received and 17 submissions were positive for IB virus by polymerase chain reaction. HRM curve analysis classified each strain as subgroup 1, 2 or 3 strain (12 submissions) or a strain that was unable to be classified (5 submissions). The 3′ untranslated region (UTR) and partial S1 gene nucleotide sequences for the 17 IB virus strains were determined and their identity with those of the relative reference strains compared to confirm the classifications generated using the HRM curve analysis. Results Of the 12 IB field viruses classified as subgroup 1, 2, or 3 using HRM curve analysis, the 3′UTR and S1 gene nucleotide sequences had identities ≥99% with the respective subgroup reference strain. Analysis of the 3′ UTR and S1 gene nucleotide sequences for the five IB virus strains that could not be classified indicated that four belonged to one of the subgroups, and one was a potential recombinant strain (between strains from subgroups 2 and 3). A novel recombinant strain was also detected. Conclusion HRM curve analysis can rapidly assign the majority of IB viruses present in field submissions to known subgroups. Importantly, HRM curve analysis also identified variant genotypes that require further investigation.  相似文献   

Spina bifida with associated malformations of the central nervous system in Dorper-cross sheep     

KA Potter  JF Weston  JS Munday  AC Johnstone 《New Zealand veterinary journal》2013,61(6):315-318

Abstract

CASE HISTORY: In 2008, six lambs within a flock of Dorpercross sheep were born with musculoskeletal and neurological disease. Clinical signs included hindlimb weakness, and urinary incontinence.

CLINICAL FINDINGS: All lambs had focal, inverted areas of alopecic skin over the caudal sacrum, and short, often kinked tails. Four affected lambs were subject to euthanasia, and necropsied. On gross examination, the arches of sacral vertebrae were absent, and spinal nerves and meninges were adherent to the overlying subcutis. Other gross lesions included narrow, elongated skulls, herniation of the occipital lobes into the caudal fossas, hydrocephalus, and syringomyelia. One lamb had coning of the cerebellar vermis, but cerebellar herniation through the foramen magnum was not identified.

DIAGNOSIS: Spina bifida, with associated malformations of the central nervous system.

CLINICAL RELEVANCE: Examination of breeding records suggested either an autosomal recessive or partially penetrant autosomal dominant pattern of inheritance. Because of the associated tail lesions it is proposed that the pathogenesis of this syndrome involves a defect in development of the tail bud (secondary neurulation), that tethering of the spinal cord resulted in the clinical signs, and abnormal pressure of the cerebral spinal fluid resulted in the defects in the skull and brain.  相似文献