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51.
52.
This greenhouse study aimed to analyze the impact of arbuscular mycorrhizal (AM) fungal associations on maize (Zea mays L. hybrid Pioneer 3905) in order to compare their functional compatibility and efficiency. The AM fungus species used for this study were Glomus aggregatum, G. etunicatum, G. mosseae, and G. versiforme. Shoot and leaf masses, chlorophyll, soluble protein, total and reducing sugar, carbon (C), and nitrogen (N) concentrations, and glutamine synthetase (GS) activity in the maize leaves were analyzed. The root colonization ranged from 26% to 72% depending on the AM fungus species. Leaf mass was significantly higher when maize plants were colonized with G. etunicatum in comparison to the non‐AM control. The mycorrhizal effect on dry leaf mass ranged from 15.9% to 23.9% depending on the AM species. However, the total shoot mass did not differ significantly among the treatments. The mycorrhizal treatment had a marginally significant effect on the chlorophyll concentrations in maize leaves. The protein concentration was the highest in the plants colonized with G. etunicatum and the N percentage was significantly higher in the leaves of plants colonized by G. versiforme or G. aggregatum than those with G. mosseae. However, the AM colonization did not significantly alter the GS activity among the treatments. The highest sugar concentrations were detected in the leaves of plants colonized by G. versiforme. The sugar concentrations as well as the C percentage were lower in the leaves of plants colonized by G. etunicatum compared to the other mycorrhizal treatments but the values were comparable to the non‐AM control. Our overall results suggest that the expression of the mycorrhizal potential in the maize host plants varies among AM fungal species. 相似文献
53.
da Silva Andrade G Bruhn FR Rocha CM Guimarães Ade S Gouveia AM Guimarães AM 《Veterinary parasitology》2012,188(1-2):168-171
The aim of this study was to determine the prevalence and risk factors associated with infection due to Neospora caninum in serum samples from 488 sheep originating from 63 farms in 63 municipalities distributed across eight of the twelve mesoregions of the state Minas Gerais, southeastern Brazil. For detection of N. caninum the sheep serum samples were subjected to the indirect fluorescence antibody test (IFAT ≥ 50). To identify the risk factors associated with infection due to N. caninum a questionnaire was filled out for each herd by interviewing, the individual responsible for the herd, demanding information on the general characteristics of the property. Sixty-four sheep sera (13.1%; 95% CI=10.3-16.4) presented IgG-specific anti-N. caninum antibodies with the following titers: 50 (49; 76.6%), 100 (7; 10.9%), 200 (4; 6.2%), 400 (3; 4.7%) and 800 (1; 1.6%). The prevalence of infected sheep per mesoregion ranged from 0 to 28.1%. Out of the 63 farms sampled, 31 (49.2%; 95% CI=36.4-62.1) presented at least one seropositive sheep. No significant association was found between the presence of anti-N. caninum antibodies and the risk factors evaluated on the farms, except for the mesoregion variable (p=0.004; OR=0.429; CI95%=0.182-1.008). These results indicate that there is a need for additional research to define the epidemiological importance of this parasite as a cause of reproductive problems in sheep herds in Minas Gerais. 相似文献
54.
Laurence Vaissayre Morgane Ardisson Christiane Borries Sylvain Santoni Jacques David Pierre Roumet 《Euphytica》2012,185(1):61-75
Genetic maps published for elite durum wheat (Triticum turgidum durum Desf.), were mainly related to Italian and North American pools. To provide additional information dedicated to durum elite pool,
a recombinant inbred population (217 RILs) derived form 2 French commercial varieties (Ixos and Primadur), has been mapped
with 529 loci (98 AFLPs, 67 SSRs, 1 ISBP and 363 DArTs). Genetic map spanned a total of 2,082 cM (5.7 cM/marker on average).
Recombination rate variation over the genome was documented through the analysis of six segregating populations representing
a total of 48 RILs × 6, issued from an half diallel design based on four elite lines—Neodur, Ixos, Lloyd and Primadur—including
the 2 previous genitors. Each set of three genetically connected populations (48 RILs × 3) was used to build the consensus
parental maps for each genitor. To construct the four consensus parental maps, marker ordering was determined on 217 RILs
map which was an extended population of the most polymorphic cross (Ixos × Primadur). Sizeable, recombination rate variation
has been observed between the four parental maps both at the marker pair scale and at the linkage group scale. Two of the
parents, Lloyd and Primadur, had strong, opposite effects increasing and decreasing, respectively the recombination rate.
Additional studies are proposed to increase our understanding of this variation, with the identification of environmental
or genetic factors affecting recombination rate. 相似文献
55.
56.
Stefan Schwarz Alexander Böttner Luc Goossens H. Mohamed Hafez Katrin Hartmann Martin Kaske Corinna Kehrenberg Manfred Kietzmann Dieter Klarmann Günter Klein Peter Krabisch Gabriele Luhofer Angelika Richter Bianka Schulz Claudia Sigge Karl-Heinz Waldmann Jürgen Wallmann Christiane Werckenthin 《Veterinary microbiology》2009
57.
58.
Luhofer G Böttner A Hafez HM Kaske M Kehrenberg C Kietzmann M Klarmann D Klein G Krabisch P Kühn T Richter A Sigge C Traeder W Waldmann KH Wallmann J Werckenthin C Schwarz S;Arbeitsgruppe "Antibiotikaresistenz" 《Berliner und Münchener tier?rztliche Wochenschrift》2004,117(7-8):245-251
Two layouts for microtitre plates, which should serve for in-vitro susceptibility testing in routine diagnostics, have been set up by the working group "Antibiotic resistance" of the German Society for Veterinary Medicine. One of these layouts was designed for the testing of bacteria from cases of mastitis and the other for bacteria from infections in large food-producing animals. The choice of the antimicrobial agents and their concentrations to be included in these layouts were based on (1) the bacteria frequently associated with the respective diseases/animals, (2) the antimicrobial agents licensed for therapeutic use in these diseases/animals, (3) the currently available breakpoints, and (4) cross-resistances between the antimicrobial agerts so far known to occur in the respective bacteria. 相似文献
59.
Pauls J Nolte C Forterre F Brunnberg L 《Berliner und Münchener tier?rztliche Wochenschrift》2004,117(7-8):341-352
Despite the numerous available possibilities for the surgical treatment of peripheral nerve lesions found in the dog, the success of these treatments is often unsatisfactory. It has been proven that Schwann cells (SC) have a positive influence on the regeneration of nerve stumps. Implanting a guidance channel seeded with autologous SC at the lesion site could be a new therapeutic approach. The aim of this research was to investigate the in vitro cultivation and expansion of canine SC as the main requirement for the treatment referred to above. Biopsies were carried out on 17 nerve samples originating from dogs of different breed, age, gender and condition. The reexplantation method was employed, followed by dissociation using hyaluronidase, collagenase and trypsin and further expansion. The samples were divided into six groups which were treated with a varying combination of mitogens (forskolin, bovine PEX, choleratoxin, heregulin). To obtain the quantities of SC, the specimens were immunostained by a p75-antibody. By employing a growing number of agents it was possible to obtain an increase in both the quantity of cells and purity of cultures. A maximum of 16x10(5) cells per millilitre of suspension was achieved. The largest SC purity measured 27.1%. The maximum SC quantity achieved was 43.3x10(4) SC per millilitre. 相似文献
60.
Arsenault J Girard C Dubreuil P Bélanger D 《The Canadian veterinary journal. La revue veterinaire canadienne》2004,45(4):312-314
A study was conducted to estimate the seroprevalence of Brucella ovis infection in rams in the Estrie and Bas-Saint-Laurent regions (Quebec). Rams sera (n = 258) were serologically evaluated from 224 rams in 30 commercial flocks and from 34 rams at 2 slaughterhouses by using an enzyme linked immunosorbent assay. Epididymides and testes were examined by palpation on farms and microscopically for culled rams. No ram was seropositive to Brucella ovis or had lesions suggestive of brucellosis from the farm or slaughterhouse surveys. 相似文献