Flood damage assessment on rice crop in the Stung Sen River Basin of Cambodia     

Chung  Sarit  Takeuchi  Junichiro  Fujihara  Masayuki  Oeurng  Chantha 《Paddy and Water Environment》2019,17(2):255-263

Paddy and Water Environment - In Cambodia, rice production is important for the majority of rural populations as the main source of income. However, historical extreme flood events, especially the...  相似文献   

Synthesis of conjugated linoleic acid by human-derived Bifidobacterium breve LMC 017: utilization as a functional starter culture for milk fermentation     

Chung SH  Kim IH  Park HG  Kang HS  Yoon CS  Jeong HY  Choi NJ  Kwon EG  Kim YJ 《Journal of agricultural and food chemistry》2008,56(9):3311-3316

This study was performed to discover bifidobacteria isolated from human intestines that optimally convert linoleic acid (LA) to conjugated linoleic acid (CLA) and to optimize the culture conditions of milk fermentation. One hundred and fifty neonatal bifidobacteria were screened for CLA-producing ability, and Bifidobacterium breve LMC 017 was selected as it showed about 90% conversion of free LA in MRS broth. The selected strain showed resistance at 0.5% LA in microaerophillic conditions. When monolinolein (LA 90%) was used as a substrate for CLA production, the conversion rate was lower compared to free LA, but the growth rate was unaffected during the milk fermentation. There was no significant difference in CLA production between aerobic and anaerobic conditions, and little decline in CLA was shown after the maximal CLA level had been reached. CLA production increased by 80% with 24 h of incubation in milk containing additional skim milk (5%), where the proteins may have facilitated the production of CLA by enhancing the interaction of substrate with the bacteria. CLA production did not decline after 9 h of fermentation and an additional 12 weeks of storage with other commercial starters. This demonstrates the possibility of using this strain as a costarter in the production of CLA-enriched yogurt.  相似文献   

Utilization of monolinolein as a substrate for conjugated linoleic acid production by Bifidobacterium breve LMC 520 of human neonatal origin     

Choi NJ  Park HG  Kim YJ  Kim IH  Kang HS  Yoon CS  Yoon HG  Park SI  Lee JW  Chung SH 《Journal of agricultural and food chemistry》2008,56(22):10908-10912

This study was designed to isolate bifidobacteria from human intestines that efficiently converts monolinolein, a monoglyceride form of linoleic acid, into conjugated linoleic acid (CLA), as well as to optimize culture conditions for improving CLA production during milk fermentation. Among 150 screened neonatal bifidobacteria, Bifidobacterium breve LMC 520 showed the highest CLA-producing ability and was tested with different types of fat substrates at various concentrations to determine the optimal conditions for CLA production. Monolinolein was tested as a substrate for CLA production. The incubation time optimized for CLA production was 24 h, and CLA production was proportionally increased with monolinolein concentration. The incubation of LMC 520 with commercial starter strains caused minimal reduction in CLA production. Our results demonstrate that the CLA-producing ability of B. breve LMC 520 could offer beneficial effects when utilized as a starter culture for the development of functional dairy products.  相似文献   

Protective effect of anthocyanins from black soybean seed coats on UVB-induced apoptotic cell death in vitro and in vivo   总被引：1，自引：0，他引：1  

Tsoyi K  Park HB  Kim YM  Chung JI  Shin SC  Shim HJ  Lee WS  Seo HG  Lee JH  Chang KC  Kim HJ 《Journal of agricultural and food chemistry》2008,56(22):10600-10605

UVB radiation proves to be one of the most relevant environmental risks because of its hazardous effects, such as premature skin aging and especially skin photocarcinogenesis. Anthocyanins, water-soluble pigments present in plants, are known to be powerful antioxidants that help protect plants from UV damage. In this study, we aimed at investigating the protective effect of anthocyanins from black soybean [Glycine max (L.) Merr] seed coats on UVB-induced apoptosis, and furthermore, we investigated the molecular mechanism responsible for regulation of apoptosis in vitro and in vivo. Pretreatment with anthocyanins reduced UVB-induced reactive oxygen species levels and inhibited UVB-induced apoptotic cell death through the prevention of caspase-3 pathway activation and reduction of proapoptotic Bax protein levels. UVB irradiation induced apoptotic cell death, which was inhibited by topical application of anthocyanins in hairless mice. It is concluded that anthocyanins from the seed coat of black soybeans may be useful compounds to modulate UVB-induced photoaging.  相似文献   

Proteome analysis of greenhouse-cultured lettuce with the natural soil mineral conditioner illite     

Jong-Soon Choi  Seong-Woo Cho  Tae-Seon Kim  Kun Cho  Seok-Soon Han  Hong-Ki Kim  Sun-Hee Woo  Keun-Yook Chung 《Soil biology & biochemistry》2008,40(6):1370-1378

The natural soil mineral conditioner illite effectively improved the germination and growth of lettuce when applied in particulate or powdered form. When illite was given in particulate and powdered forms, the germination rate of lettuce seeds improved remarkably up to 93% and 133%, respectively. Contrary to the developmental effects, the growth rate of lettuce treated with particulate illite improved slightly by 23%; powdered illite had no significant effects on lettuce growth rate. Thus, illite primarily affects seed germination rather than the growth of lettuce. To examine illite-induced proteins related to lettuce growth, differentially expressed proteins in lettuce leaves were analyzed by two-dimensional gel electrophoresis (2-DE) and matrix-assisted laser desorption ionization-time of flight/time of flight mass spectrometry (MALDI-TOF/TOF MS) followed by Mascot search. From the proteomic analysis, five down-regulated proteins were identified related to storage protein, carbon metabolism and energy conversion. Three up-regulated proteins were related to energy production/conversion and carbon fixation. These results demonstrate that illite treatment as a soil conditioner helps lettuce seed germination and lettuce growth by regulating carbon metabolic flux.  相似文献   

Radiation-Induced Breeding in Camelina Sativa     

Chung  Yong Suk  Silva  Renato Rodrigues  Park  Minwoo  Lee  Sanghyeob 《Journal of Crop Science and Biotechnology》2019,22(1):17-20

Journal of Crop Science and Biotechnology - The ionizing radiation methods, including X-rays, gamma rays, and neutrons to create variation is well established and used in major crops. Many...  相似文献   

Cloning and characterization of 5'-untranslated region of porcine beta casein gene (CSN2)     

Lee P  Chung HK  Lee HG  Lee HC  Woo JS  Lee S  Jo SJ  Chang WK  Lee HT  Kwon M  Park JK 《Domestic animal endocrinology》2008,35(3):245-253

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Reduced immunoreactivity of tyrosine hydroxylase in the hypothalamic paraventricular nucleus of the seizure sensitive gerbil     

Lee CH  Choi JH  Chung DW  Hwang IK  Yoon YS  Won MH  Lee IS 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2008,70(6):645-648

We compared the immunoreactivity and numbers of tyrosine hydroxylase (TH) immunoreactive neurons and neuropil in the paraventricular nucleus (PVN) of the hypothalamus between the seizure sensitive (SS) and seizure resistant (SR) gerbils. The distributional pattern of TH immunoreactivity was similar in both groups: TH immunoreactivity was seen mainly in magnocellular neurons of the PVN. However, total TH immunoreactivity in the neurons and neuropil in the SS gerbils was significantly lower than that in the SR gerbils. In addition, the number of TH immunoreactive neurons in the SS gerbils was also much lower than those in the SR gerbils. These results indicate that SS gerbils have a low TH immunoreactivity in the hypothalamic PVN compared with that in SR gerbils.  相似文献   

The rate of Salmonella spp. infection in zoo animals at Seoul Grand Park, Korea     

Y. H. Jang  S. J. Lee  J. G. Lim  H. S. Lee  T. J. Kim  J. H. Park  B. H. Chung  N. H. Choe 《Journal of veterinary science (Suw?n-si, Korea)》2008,9(2):177-181

Salmonellosis is an important zoonotic disease that affects both people and animals. The incidence of reptile-associated salmonellosis has increased in Western countries due to the increasing popularity of reptiles as pets. In Korea, where reptiles are not popular as pets, many zoos offer programs in which people have contact with animals, including reptiles. So, we determined the rate of Salmonella spp. infection in animals by taking anal swabs from 294 animals at Seoul Grand Park. Salmonella spp. were isolated from 14 of 46 reptiles (30.4%), 1 of 15 birds (6.7%) and 2 of 233 mammals (0.9%). These findings indicate that vigilance is required for determining the presence of zoonotic pathogen infections in zoo animals and contamination of animal facilities to prevent human infection with zoonotic diseases from zoo facilities and animal exhibitions. In addition, prevention of human infection requires proper education about personal hygiene.  相似文献   

Serum immunoglobulin fused interferon-�� inhibited tumor growth in athymic mice bearing colon 26 adenocarcinoma cells     

Jun-Sung Kim  Kyeong Nam Yu  Mi Suk Noh  Min-Ah Woo  Sung-Jin Park  Jin Hong Park  Jin Hua  Hyun Sun Cho  Soon Kyung Hwang  Eun-Sun Lee  Youn-Sun Chung  In-Young Choi  Se-Chang Kwon  Myung-Haing Cho 《Journal of veterinary science (Suw?n-si, Korea)》2008,9(1):45-50

Interferon (IFN) has therapeutic potential for a wide range of infectious and proliferative disorders. However, the half-life of IFN is too short to have a stable therapeutic effect. To overcome this problem, serum immunoglobulin has been fused to IFN. In this study, the efficacy of serum immunoglobulin fused INFs (si-IFN1 and si-IFN2) was evaluated on athymic mice bearing colon 26 adenocarcinoma cells. Seven days after the implantation of tumor cells, each group of mice was injected once a week with si-IFN1 and si-IFN2 at two different concentrations (10 × : 30 µg/kg and 50 × : 150 µg/kg). A slight anti-tumoral effect was observed in all 10 × groups compared to the control. In the 50 × groups, however, si-IFN1 and si-IFN2 showed significant anti- tumoral effects compared to the control. To gain more information on the mechanisms associated with the decrease of tumor size, a Western blot assay of apoptosis-related molecules was performed. The protein expression of cytochrome c, caspase 9, 6, and 3 were increased by si-IFN1 and si-IFN2. These 2 IFNs also increased the expressions of p53, p21, Bax and Bad. Interestingly, si-IFN1 and si-IFN2 decreased the expression of VEGF-β. Taken together, serum immunoglobulin fused IFNs increased therapeutic efficacy under current experimental condition.  相似文献