Immunolocalization of Melatonin and Follicle‐Stimulating Hormone Receptors in Caprine Ovaries and their Effects During in vitro Development of Isolated Pre‐Antral Follicles     

VRP Barros  AYP Cavalcante  TJS Macedo  RS Barberino  TLB Lins  BB Gouveia  VG Menezes  MAA Queiroz  VR Araújo  RC Palheta Jr  MCP Leite  MHT Matos 《Reproduction in domestic animals》2013,48(6):1025-1033

The expression of melatonin type 1 (MT1) and FSH (FSHR) receptors in caprine ovaries and the effects of these hormones on the in vitro development of isolated pre‐antral follicles were evaluated. Follicles (≤200 μm) were cultured for 12 days in α‐MEM (control) or melatonin (100 or 1000 pg/ml) or sequential melatonin medium (100 pg/ml: from day 0 to day 6; 1000 pg/ml: from day 6 to day 12; experiment 1) and in control or sequential FSH (100 ng/ml from day 0 to day 6; 500 ng/ml from day 6 to day 12) or sequential melatonin or this latter plus sequential FSH (experiment 2). MT1 and FSHR expressions were observed in granulosa cells from secondary and antral follicles. The oocytes from primordial and primary follicles also express FSHR. Sequential melatonin increased the percentage of normal follicles and oocyte recovery compared with the control or melatonin (1000 pg/ml) at day 12. In experiment 2, all the treatments increased the normal follicles and growth compared with the control. In conclusion, this study demonstrated the presence of MT1 and FSHR in caprine ovaries. The addition of increased concentrations of melatonin (sequential medium) or FSH can be used to promote the in vitro development of caprine pre‐antral follicles.  相似文献   

Using acellular aortic matrix to repair umbilical hernias of calves     

V Kumar  N Kumar  AK Gangwar  AC Saxena 《Australian veterinary journal》2013,91(6):251-253

  相似文献   

Causes of perinatal deaths in foals in New Zealand in the 2007 and 2008 seasons     

AG Wolfe  AC Johnstone  IG Mayhew 《New Zealand veterinary journal》2013,61(1)

  相似文献   

Removal of Malachite Green from Aqueous Medium Employing Polyurethane Foam as Adsorbent and Sodium Dodecylsulfate as Carrier     

Breno Tenório Leite  Nicolle Figueira Robaina  Luis Gustavo Teixeira dos Reis  Annibal Duarte Pereira Netto  Ricardo Jorgensen Cassella 《Water, air, and soil pollution》2012,223(3):1303-1313

The present work describes a detailed study about the adsorption of malachite green (MG) by a polyether-type polyurethane foam (PUF) using sodium dodecylsulfate (SDS) as a carrier. The adsorption process was based on the formation of a hydrophobic ionic-pair between the MG cationic dye and the dodecylsulfate anion, which presented high affinity for the PUF. The manifold employed in the study was built up by adjusting a cylinder of PUF with 200 mg in the arm of an overhead stirrer, which was soaked (and stirred) in the solution containing the dye and SDS. The adsorption process was characterized in relation to equilibrium and kinetic aspects. Langmuir (r ² = 0.842) and Freundlich (r ² = 0.996) isotherms were also employed for modeling the system as well as the Nernst partition law (r ² = 0.999). A study about the recovery of MG and the PUF regeneration was conducted, and the acetonitrile was the most efficient solvent for the desorption of the adsorbed ionic pair. The obtained results showed that the concentration of SDS added to the medium plays an important role on the adsorption process, which can be better described by employing a second-order kinetic model.  相似文献   

The effect of land use on aboveground biomass and soil quality indicators in spontaneous forests and agroforests of eastern Amazonia     

Márcio Fernandes Alves Leite  Ronildson Lima Luz  Karol Henry Mavisoy Muchavisoy  Marcelo Luís Corrêa Zelarayán  Ernesto Gomez Cardoso  Flávio Henrique Reis Moraes  Guillaume Xavier Rousseau  Christoph Gehring 《Agroforestry Systems》2016,90(6):1009-1023

  相似文献   

Sublethal doses of fipronil intensify synapsin immunostaining in Atta sexdens rubropilosa (Hymenoptera: Formicidae) brains          下载免费PDF全文

Priscila Cintra‐Socolowski  Thaisa C Roat  Roberta CF Nocelli  Pablo H Nunes  Rafael AC Ferreira  Osmar Malaspina  Odair C Bueno 《Pest management science》2016,72(5):907-912

  相似文献   

Chromosome markers confirm origin of Heliconia hybrids and triploids     

Marília Gabriela de Santana Costa  Brunno Sérgio Ferreira Leite  Vivian Loges  Ebenézer Bernardes Correia Silva  Andreza Santos da Costa  Walma Nogueira Ramos Guimarães  Ana Christina Brasileiro-Vidal 《Euphytica》2016,207(3):501-514

  相似文献   

Anadenanthera colubrina (Vell.) Brenan produces steroidal substances that are active against Alternaria alternata (Fr.) Keissler and that may bind to oxysterol‐binding proteins     

Viviane AC Campos  Fabiano J Perina  Eduardo Alves  Jaqueline Sartorelli  Amanda M Moura  Denilson F Oliveira 《Pest management science》2014,70(12):1815-1822

  相似文献   

Antineoplastic activity of selected constituents of Duguetia glabriuscula     

Matos MF  Leite LI  Brustolim D  de Siqueira JM  Carollo CA  Hellmann AR  Pereira NF  da Silva DB 《Fitoterapia》2006,77(3):227-229

The cytotoxic effects of seven constituents isolated from Duguetia glabriuscula were evaluated against Hep2 human larynx carcinoma cells. The cytotoxicity exhibited by beta-sitosterol was as strong as that of cis-platin. (+)-Alloaromadendran-10,14beta-diol caused inhibition of cellular growth with IC50 values lower than 25 microg/ml, a feature that was considered as revealing significant activity. Polycarpol showed borderline cytotoxicity, whereas the other compounds were inactive.  相似文献   

Economical and financial analysis of lamb finishing fed with diets formulated according to the NRC (1985) and the NRC (2007)     

Marcos Cláudio Pinheiro Rogério  Eliane Minervina de Castro  Espedito Cezário Martins  Jomar Patrício Monteiro  Kleibe de Moraes Silva  Magno José Duarte Cândido  Tereza Cristina Lacerda Gomes  Antoine Francis Roux Bloc  Ângela Maria de Vasconcelos  Eneas Reis Leite  Hélio Henrique Araújo Costa 《Tropical animal health and production》2012,45(1):259-266

This study compares both versions of the nutritional requirement system determined by the National Research Council (NRC) version 1985 (NRC85) and NRC version 2007 (NRC07), for finishing lambs in feedlots. Nineteen crossbred lambs were divided in four groups representing four experimental treatments: one diet according to NRC85 and three diets according to NRC07. The diets recommended by NRC07 considers crude protein intake relative to ruminal undegradable protein at 20, 40, and 60 % levels (NRC07/20, NRC07/40, and NRC07/60). Diets were composed of Brazilian semi-arid native grass silage, soybean meal, corn, annatto byproduct, and limestone. Purchases and sales of lambs were done according to average market prices in Brazil. The economic indicators considered pointed that all treatments were viable but NRC07/20 and NRC07/60 were more profitable with similar net present values (NPVs) and internal return rates (IRRs). NRC07/20 was the best option showing an IRR of 17.20 % and a payback period (PP) of 5.07 considering a fixed annual interest rate of 6 %. Sensitivity analysis considering a 10 % raise in variable costs showed negative NPVs, IRRs inferior to the opportunity cost rates adopted and PPs that exceeded the planning horizon of 7 years for both NRC85 and NRC07/40.  相似文献