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排序方式: 共有106条查询结果,搜索用时 484 毫秒
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Yada Chutimanitsakun Alfonso Cuesta‐Marcos Shiaoman Chao Ann Corey Tanya Filichkin Scott Fisk Mathias Kolding Brigid Meints Yee‐Ling Ong Juan Ignacio Rey Andrew S. Ross Patrick M. Hayes 《Plant Breeding》2013,132(6):563-570
Barley (Hordeum vulgare) is an important component of heart‐healthy whole grain diets because it contains β‐glucan. All current US barley varieties with high β‐glucan are spring habit and have waxy starch. Winter varieties have agronomic advantages but require low‐temperature tolerance (LTT). Vernalization sensitivity (VS) is associated with higher levels of LTT. To rapidly develop fall‐sown varieties with LTT and higher grain β‐glucan, we therefore used marker‐assisted selection (MAS) at the WX and VRN‐H2 loci. The MAS‐derived lines, together with unrelated non‐waxy germplasm developed via phenotypic selection (PS), were used for a genome‐wide association scan (GWAS). The panel was phenotyped for grain β‐glucan, LTT and VS. It was genotyped with 3072 single‐nucleotide polymorphisms (SNPs) and allele‐specific primers. Marker‐assisted selection fixed target alleles at both loci but only one of the target phenotypes (higher β‐glucan percentage) was achieved. Variation for VS and LTT is attributable to (i) incomplete information about VRN‐H1 at the outset of the project and (ii) unexpected allelic variation at VRN‐H3 with a large effect on VS and LTT. 相似文献
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Westfall CS Zubieta C Herrmann J Kapp U Nanao MH Jez JM 《Science (New York, N.Y.)》2012,336(6089):1708-1711
Acyl acid amido synthetases of the GH3 family act as critical prereceptor modulators of plant hormone action; however, the molecular basis for their hormone selectivity is unclear. Here, we report the crystal structures of benzoate-specific Arabidopsis thaliana AtGH3.12/PBS3 and jasmonic acid-specific AtGH3.11/JAR1. These structures, combined with biochemical analysis, define features for the conjugation of amino acids to diverse acyl acid substrates and highlight the importance of conformational changes in the carboxyl-terminal domain for catalysis. We also identify residues forming the acyl acid binding site across the GH3 family and residues critical for amino acid recognition. Our results demonstrate how a highly adaptable three-dimensional scaffold is used for the evolution of promiscuous activity across an enzyme family for modulation of plant signaling molecules. 相似文献
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Giguère S Hernandez J Gaskin J Miller C Bowman JL 《Journal of the American Veterinary Medical Association》2003,222(6):775-781
OBJECTIVE: To evaluate WBC concentration, plasma fibrinogen concentration, and an agar gel immunodiffusion (AGID) test for early identification of Rhodococcus equi-infected foals. DESIGN: Prospective study. ANIMALS: 162 foals from a farm with enzootic R equi infection. PROCEDURE: Blood samples were obtained from each foal at 4-week intervals for measurement of WBC and plasma fibrinogen concentrations and at 2-week intervals for detection of anti-R equi antibody by an AGID assay. Diagnostic performance of WBC and fibrinogen concentrations was assessed by use of receiver operating characteristic curve analysis. For each assay, sensitivity, specificity, and predictive values were calculated at various cutoff points; bacteriologic culture of R equi from a tracheobronchial aspirate was used as the reference standard test. RESULTS: Diagnostic performance of WBC concentration was significantly higher than that of fibrinogen concentration. Sensitivity and specificity of measurement of WBC concentration at a cutoff of 13,000 cells/microL were 95.2 and 61.2%, respectively; at a cutoff of 15,000 cells/microL, sensitivity was 78.6% and specificity was 90.8%. When a positive test result was used as the cutoff, sensitivity of the AGID assay was 62.5% and specificity was 53.8%. CONCLUSION AND CLINICAL RELEVANCE: Monitoring WBC concentration is a useful approach for early detection of infected foals on farms with a high prevalence of R equi pneumonia. In contrast, serologic surveillance by use of an AGID assay is of little benefit for that purpose. 相似文献
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Corey A. Moffet Stuart P. Hardegree John T. Abatzoglou Katherine C. Hegewisch R. Ryan Reuter Roger L. Sheley M.W. Brunson G.N. Flerchinger Alex R. Boehm 《Strength and Conditioning Journal》2019,72(2):225-229
Rangeland seeding practices in the Intermountain western United States are predominantly implemented in the year immediately following wildfire for the purposes of Emergency Stabilization and Rehabilitation (ESR). This necessarily links restoration and rehabilitation outcomes to the probability of a single year providing sufficiently favorable microclimatic conditions for desirable plant establishment. Field research studies in rangeland restoration are also typically of limited duration, and published results may not represent the full spectrum of conditions likely to be experienced at a given site. We propose that location-specific and temporal weather analysis may enhance the interpretation of historical planting data, support expanded inferences from short-term field studies, and facilitate meta-analysis of diverse field studies in rangeland restoration. We describe access and use of new databases and tools that can be used to characterize and rank weather and soil-microclimatic variables and suggest some standard graphs and weather metrics to establish a longer-term perspective for the interpretation of rangeland restoration outcomes. Tools of this type may also be useful in the interpretation of a wide range of agricultural and natural resource applications that are driven by similar weather inputs, particularly in arid and semiarid systems that exhibit high annual and seasonal variability in precipitation and temperature. 相似文献
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Stuart P. Hardegree Roger A. Sheley Mark W. Brunson Michael H. Taylor Corey A. Moffet 《Strength and Conditioning Journal》2019,72(2):217-224
Millions of hectares of sagebrush/bunchgrass rangeland in the western United States are undergoing type conversion to systems dominated by introduced annual grasses that proliferate after wildfire. Postfire rehabilitation and restoration are problematic in these complex systems, but restoration difficulties are exacerbated by high annual and seasonal variability in precipitation and persistent drought. Successful restoration of compositional, structural, and functional diversity in these weather-limited systems may require relatively long-term, iterative management that incorporates flexibility in the definition of the aspirational/goal state. Restoration planning should also explicitly accommodate a lack of predictability of individual-year management results and expectations of only partial success of individual-year management treatments. This planning environment may require rapid assessment and contingency planning in the short term but also long-term persistence to overcome expected failures and setbacks. New methodologies are needed to increase biodiversity without damaging previously established plants, and new metrics need to be developed to monitor successional trajectories between initial and multiple-potential goal states. 相似文献
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Genevieve M. Forster Jonathan Stockman Noelle Noyes Adam L. Heuberger Corey D. Broeckling Collin M. Bantle Elizabeth P. Ryan 《Topics in companion animal medicine》2018,33(4):126-135
The aim of this study was to compare fecal microbiome, plasma, fecal and urine metabolomes, and serum biochemistry of adult companion dogs according to body condition scores. Blood, serum/plasma, urine, and fecal samples were collected from 66 clinically healthy, adult companion dogs of either normal weight (NW), overweight (OW), or obese dogs (OB). analyses included fecal microbiome analyses via 16S ribosomal RNA gene amplicon; sequencing, nontargeted plasma, fecal, and urine metabolomics using liquid chromatography/gas chromatography-mass; spectrometry, and serum biochemistry for each dog. Few significant differences in serum biochemistry and fecal microbiome Operational Taxonomic Unit (OTU) were found between weight groups and there was high OTU variation between individual dogs. NW dogs had higher relative abundance of the genus Eubacterium (log-fold change 4.3, adjusted P value?=?.003) and lower relative abundance of the family Bifidobacteriaceae (log-fold change ?3.6, adjusted P value?=?.02) compared to OB dogs. The microbiome of NW dogs had higher OTU richness compared with OB dogs. Metabolome analysis showed 185 plasma, 37 fecal, and 45 urine metabolites that significantly differed between NW and OW or OB dogs. There were notable significant differences in relative abundance of several plasma phospholipid moieties and fecal volatile fatty acids between weight phenotypes. The combinations of host and gut microbiota and metabolic shifts suggest a pattern that could help detection of early metabolic changes in overweight dogs before the development of obesity related disease. The results of this study support the need for continued investigation into sensitive measures of metabolic aberrancies in overweight dogs. 相似文献