Discovering new areas of veterinary science through qualitative research interviews: introductory concepts for veterinarians          下载免费PDF全文

CF May 《Australian veterinary journal》2018,96(8):278-284

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Melatonin reduces apoptotic cells,SOD2 and HSPB1 and improves the in vitro production and quality of bovine blastocysts          下载免费PDF全文

TC Marques  EC da Silva Santos  TO Diesel  LO Leme  CF Martins  MAN Dode  BG Alves  FPH Costa  EB de Oliveira  ML Gambarini 《Reproduction in domestic animals》2018,53(1):226-236

Effects of adding different concentrations of melatonin (10^?7, 10^?9 and 10^?11 M) to maturation (Experiment 1; Control, IVM  + 10^?7, IVM  + 10^?9, IVM  + 10^?11) and culture media (Experiment 2; Control, IVC  + 10^?7, IVC  + 10^?9, IVC  + 10^?11) were evaluated on in vitro bovine embryonic development. The optimal concentration of melatonin (10^?9 M) from Experiments 1–2 was tested in both maturation and/or culture media of Experiment 3 (Control, IVM  + 10^?9, IVC  + 10^?9, IVM /IVC  + 10^?9). In Experiment 1, maturated oocytes from Control and IVM  + 10^?9 treatments showed increased glutathione content, mitochondrial membrane potential and percentage of Grade I blastocysts (40.6% and 43%, respectively). In Experiment 2, an increase in the percentage of Grade I blastocysts was detected in IVC  + 10^?7 (43.5%; 56.7%) and IVC  + 10^?9 (47.4%; 57.4%). Moreover, a lower number and percentage of apoptotic cells in blastocysts were observed in the IVC  + 10^?9 group compared to Control (3.8 ± 0.6; 3.6% versus 6.1 ± 0.6; 5.3%). In Experiment 3, the IVC  + 10^?9 treatment increased percentage of Grade I blastocysts with a lower number of apoptotic cells compared to IVM /IVC  + 10^?9 group (52.6%; 3.0 ± 0.5 versus 46.0%; 5.4 ± 1.0). The IVC  + 10^?9 treatment also had a higher mRNA expression of antioxidant gene (SOD 2) compared to the Control, as well as the heat shock protein (HSPB 1) compared to the IVM  + 10^?9. Reactive oxygen species production was greater in the IVM /IVC  + 10^?9 treatment group. In conclusion, the 10^?9 M concentration of melatonin and the in vitro production phase in which it is used directly affected embryonic development and quality.  相似文献   

Mitteilung über Schadmilben im Vorderen Orient     

Saba  F. 《Journal of pest science》1964,37(2):17-19

Zusammenfassung Die wichtigsten Milben von wirtschaftlicher Bedeutung im Libanon, Jordanien und der südlichen Türkei werden aufgeführt. Es handelt sich auf Äpfeln um:Tetranychus urticae, Metatetranychus ulmi, Tetranychus cinnabarinus, Bryobia rubrioculus; aufCitrus sp. um:Phyllocoptruta oleivora, Aceria sheldoni ,Eutetranychus orientalis, Brevipalpus obovatus, T. cinnabarinus: auf Gemüsearten um:Tetranychus urticae, T. cinnabarinus; auf Wein um:Eriophyes vitis; auf Granatäpfeln um:Eriophyes granati.Die Orte und Daten der Befunde werden angegeben.

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Summary The mites of economic importance in the Lebanons, Jordan and South-Turkey are listed with the dates and places in which they were found. On apples were found:Tetranychus urticae, Metatetranychus ulmi, Tetranychus cinnabarinus, Bryobia rubrioculus; onCitrus sp.: Phyllocoptruta oleivora, Aceria sheldoni, Eutetranychus orientalis, Brevipalpus obovatus, T. cinnabarinus; on vegetables:T. cinnabarinus, Tetranychus urticae; on vines:Eriophyes vitis; on pomegranates:Eriophyes granati.

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Résumé Les acariens d'une importance èconomique au Liban, en Jordanie et au sud de la Turquie sont donné avec les dates et les endroits où ils ont ètè trouvés. Sur pommiers on a trouvé:Tetranychus urticae, Metatetranychus ulmi, Tetranychus cinnabarinus, Bryobia rubrioculus; sur agrumes:Phyllocoptruta oleivora, Aceria sheldoni, Eutetranychus orientalis, Brevipalpus obovatus, T. cinnabarinus; sur légumes;Tetranychus urticae, T. cinnabarinus; sur les vignes:Eriophyes vitis; sur les grenades:Eriophyes granati.

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Specific developmental stages of gametogenesis for radiosensitivity and mutagenesis in rice     

Y. Kowyama  T. Saba  T. Tsuji  T. Kawase 《Euphytica》1994,80(1-2):27-38

Summary Developmental stages during gametogenesis of rice were histologically examined in the period from differentiation of reproductive organs to anthesis. Plants were exposed to acute X-rays of 20 Gy. Radiosensitivity and mutation frequency were investigated in relation to the developmental stages of reproductive organs. The most radiosensitive stage, as measured by reduction of the M1 pollen-and seed-fertilities, was the last premeiotic interphase. Mutations induced at different developmental stages were scored in M3 strains. Sterility mutants and short-culm mutants were most frequently observed. Grain shape, panicle morphology, heading-date and endosperm character mutants were induced at a relatively low frequency. The overall mutation frequency varied with the developmental stage at the time of irradiation. The highest overall mutation frequency was observed when radiation was applied 10 days before anthesis, the late tetrad stage of microspores. Radiation exposure of florets at the late tetrad stage was found to be a more efficient method of inducing a large number of mutations than radiations applied to seeds and fertilized egg cells.  相似文献   

Sirt5 is a NAD-dependent protein lysine demalonylase and desuccinylase   总被引：1，自引：0，他引：1  

Du J  Zhou Y  Su X  Yu JJ  Khan S  Jiang H  Kim J  Woo J  Kim JH  Choi BH  He B  Chen W  Zhang S  Cerione RA  Auwerx J  Hao Q  Lin H 《Science (New York, N.Y.)》2011,334(6057):806-809

Silent information regulator 2 (Sir2) proteins (sirtuins) are nicotinamide adenine dinucleotide-dependent deacetylases that regulate important biological processes. Mammals have seven sirtuins, Sirt1 to Sirt7. Four of them (Sirt4 to Sirt7) have no detectable or very weak deacetylase activity. We found that Sirt5 is an efficient protein lysine desuccinylase and demalonylase in vitro. The preference for succinyl and malonyl groups was explained by the presence of an arginine residue (Arg(105)) and tyrosine residue (Tyr(102)) in the acyl pocket of Sirt5. Several mammalian proteins were identified with mass spectrometry to have succinyl or malonyl lysine modifications. Deletion of Sirt5 in mice appeared to increase the level of succinylation on carbamoyl phosphate synthase 1, which is a known target of Sirt5. Thus, protein lysine succinylation may represent a posttranslational modification that can be reversed by Sirt5 in vivo.  相似文献   

5-Lipoxygenase expression in benign and malignant canine prostate tissues     

Goodman LA  Jarrett CL  Krunkosky TM  Budsberg SC  Northrup NC  Saba CF  LeRoy BE 《Veterinary and comparative oncology》2011,9(2):149-157

5-Lipoxygenase (5-LO) is overexpressed in human prostate carcinomas (PCs), and its inhibition decreases proliferation and induces apoptosis in prostate cancer cell lines. We hypothesized that 5-LO would be overexpressed in canine PC compared with benign prostate tissue and may be important in the pathogenesis of the disease. Immunoblot analysis of canine PC and benign prostatic hyperplasia (BPH) tissues demonstrated 5-LO expression in both. 5-LO immunohistochemical staining was not significantly different within the stromal or epithelial components of canine primary PC, BPH or suppurative prostatitis, suggesting that differential expression of this enzyme does not occur in these conditions. The percentage of tumour cells expressing 5-LO was significantly lower in metastatic PC lesions compared with primary PC (P < 0.0001). This decreased expression may indicate down-regulation or altered expression of the enzyme with progression of canine PC to a metastatic phenotype.  相似文献   

Orthodromic Reciprocating Tachycardia and Heart Failure in a Dog With a Concealed Posteroseptal Accessory Pathway     

Clarke E. Atkins  Ronald Kanter  Kathy Wright  Ziad Saba  Catherine Baty  Cliff Swanson  Stephen Bai  Bruce W. Keene 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》1995,9(1):43-49

A 4-month-old male Labrador Retriever was presented for recurrent bouts of pulmonary edema associated with tachycardia. Initial physical examination and echocardiography were unremarkable, and the electrocardiogram revealed only an intraventricular conduction disturbance. Subsequent recordings showed paroxysmal supraventricular tachycardia (SVT) (340 beats/min), which consistently produced pulmonary edema. The supraventricular tachycardia was unresponsive to adenosine, esmolol, and propranolol; was variably and transiently responsive to various vagal maneuvers and precordial thumps; and was always responsive to IV diltiazem. Multiple life-threatening episodes of SVT occurred, however, despite the chronic administration of oral diltiazem, propranolol, and procainamide. Diastolic cardiac dysfunction was documented by Doppler echocardiography and was thought to contribute to the development of pulmonary edema. A subsequent electrophysiologic study confirmed the presence of an atrioventricular posteroseptal accessory pathway that participated in orthodromic reciprocating tachycardia. This pathway was determined to conduct only in the retrograde direction ("concealed accessory pathway"). Intraoperative IV procainamide titration terminated the arrhythmia, which could not be reinduced when procainamide blood concentration approximated 20 μg/dL. Increasing the oral procainamide dose to achieve such plasma concentrations was successful in eliminating orthodromic reciprocating tachycardia, preventing heart failure, and returning Doppler indices of diastolic function to normal.  相似文献   

Outcome of 19 dogs with parathyroid carcinoma after surgical excision     

Sawyer ES  Northrup NC  Schmiedt CW  Culp WT  Rassnick KM  Garrett LD  Selting KA  Saba CF  Howerth EW 《Veterinary and comparative oncology》2012,10(1):57-64

Parathyroid carcinoma (PTC) is rare in dogs and there is little information documenting its treatment and prognosis. The objective of this study was to describe the outcome of dogs with PTC treated with surgical excision. Medical records of 19 dogs undergoing surgical excision of PTC between 1990 and 2010 were reviewed retrospectively. Dogs were presented for clinical hypercalcaemia or incidental hypercalcaemia noted by referring veterinarians on routine serum chemistry profiles. A parathyroid nodule was identified with cervical ultrasound in 17/17 dogs. Hypercalcaemia resolved in 18/19 dogs within 4 days postoperatively. Nine developed hypocalcaemia. None were confirmed to develop recurrent or metastatic PTC. The only death associated with PTC was a dog that was euthanized for intractable hypocalcaemia 9 days after surgery. Estimated 1-, 2- and 3-year survival rates were 72, 37 and 30%, respectively. Excision of PTC results in resolution of hypercalcaemia and excellent tumour control.  相似文献   

Phase II clinical evaluation of lomustine chemotherapy for feline vaccine‐associated sarcoma     

C. F. Saba  D. M. Vail  D. H. Thamm 《Veterinary and comparative oncology》2012,10(4):283-291

Treatment of feline vaccine‐associated sarcoma (VAS) is challenging, in part due to the high likelihood of tumour recurrence despite aggressive local therapy. Lomustine is potentially an attractive agent to add to the current treatment armamentarium. In this de‐escalating phase I/II prospective trial, 28 cats with measurable VAS were treated at target dosages of 38–60 mg m^?2 every 3 weeks until disease progression. The overall response rate was 25%, with a median progression‐free survival and median duration of response of 60.5 and 82.5 days, respectively. Haematologic toxicity, specifically cumulative neutropenia, was significant, and dose reductions and treatment delays were common. Although these data support further investigation of lomustine for the treatment of VAS, safe, multidosing protocols must first be determined.  相似文献   

Association between p53?Codon 72 (Arg72Pro) Polymorphism and Primary Open-Angle Glaucoma in Iranian Patients     

Hossein Neamatzadeh  Reza Soleimanizad  Masoud Zare-Shehneh  Saba Gharibi  Abolfazl Shekari  Amir Bahman Rahimzadeh 《Iranian Biomedical Journal》2015,19(1):51-56

Background: Glaucomatous neuropathy is a type of cell death due to apoptosis. The p53 gene is one of the regulatory genes of apoptosis. Recently, the association between the p53 gene encoding for proline at codon 72 and primary open-angle glaucoma (POAG) has been studied in some ethnic groups. This study is the first association analysis of POAG and p53 codon 72 polymorphism in Iranian patients. Methods: A cohort of 65 unrelated patients with POAG (age range from 12-62 years, mean ± SD of 40.16 ± 17.51 years) and 65 unrelated control subjects (without glaucoma, age range of 14-63 years, mean ± SD of 35.64 ± 13.61 years) were selected. In Iranian POAG patients and normal healthy controls, the p53 codon 72 polymorphism in exon 4 was amplified using polymerase chain reaction. The amplified DNA fragments were digested with the BstUI restriction enzyme, and the digestion patterns were used to identify the alleles for the polymorphic site. Results: Comparisons revealed significant differences in allele and genotype frequencies of Pro72Arg between POAG patients and control group. A higher risk of POAG was associated with allele Pro (OR = 2.1, 95% CI = 1.2–3.4) and genotype Pro/Pro (OR = 3.9, 95% CI = 0.13-12.7). Conclusion: The p53 Pro72 allele was more frequent in Iranian POAG patients than in the control group (P<0.05). The present findings show that the individuals with the Pro/Pro genotype may be more likely to develop POAG. However, additional studies are necessary to confirm this association. Key Words: Primary open-angle glaucoma (POAG), Glaucoma, p53, Codon 72, Iran  相似文献