紫花苜蓿9个盐响应相关基因表达模式     

张亚玲  张攀  尹航  王洋  孙杰  吴清莹  付佳琦  崔国文 《草业科学》2018,35(5):1057-1066

紫花苜蓿(Medicago sativa)是世界上种植面积最大、应用最广泛的豆科牧草。由于其耐盐性中等,其在我国北方地区的产量和种植受到土壤盐渍化的限制。因此提高紫花苜蓿的耐盐性具有重要的科学和生产意义。为此,以龙牧801紫花苜蓿(M.sativa‘Longmu 801’)为试验材料,采用实时荧光定量PCR技术分析了不同浓度NaCl胁迫下9个盐胁迫蛋白质组筛选出的盐响应相关基因的表达模式。结果显示,处理时间和处理浓度对9个基因的相对表达量均有显著性影响,表明这9个基因均在紫花苜蓿盐胁迫应答中发挥着一定作用。处理1h时,G6PI、ABP19a、Trx-h1、PR bet 1、FBPA、6PGDH和ALDH这7个基因的相对表达量在不同NaCl胁迫浓度处理的紫花苜蓿中均显著上调,RRM和GDPD在NaCl处理2h后开始上调。除G6PI基因,其他8个基因在0.4%NaCl处理下相对表达量显著高于0.2%和0.8%NaCl处理。这些基因参与糖代谢、信号转导和胁迫响应。以上结果表明,紫花苜蓿的耐盐性极其复杂,涉及到多基因的表达和代谢通路的调控。研究结果有助于全面研究并了解紫花苜蓿的盐响应相关基因的表达模式,促进紫花苜蓿耐盐分子育种。  相似文献   

高寒牧区两个燕麦品种的产量与品质比较   总被引：3，自引：0，他引：3  

崔雄雄  侯扶江  常生华  王召锋 《草业科学》2018,35(6):1489-1495

在我国青藏高原高寒牧区,栽培草地种植主要以燕麦(Avena sativa)为主,但当地现有的品种产草量较低,缺乏高产优质的燕麦品种。为了进一步提高高寒牧区燕麦产量和品质,满足当地的生产需要,于2013-2016年期间从青海省引进燕麦新品种"林纳"在碌曲县进行试验种植,并和当地现有品种"陇燕1号"的农艺性状及营养价值进行比较研究。结果表明,"林纳"燕麦生育期短,成熟快;其鲜、干草产量显著高于当地现有燕麦品种"陇燕1号"(P0.05),分别增产41%和38%。"林纳"粗蛋白(CP)、钙(Ca)和磷(P)含量较高,但差异不显著(P0.05);而粗脂肪(EE)含量显著高于当地品种(P0.05);酸性洗涤纤维(ADF)和中性洗涤纤维(NDF)低于当地品种,其中,中性洗涤纤维(NDF)含量差异显著(P0.05)。总之,"林纳"燕麦的生产力和营养品质均优于当地品种,具有良好的应用前景,可作为该地区乃至整个高寒牧区的优质燕麦品种推广种植。  相似文献   

猪伪狂犬病毒gB抗原侧向层析快速检测试纸条的研制     

王华俊  班付国  赵雪丽  谢彩华  闫若潜  王淑娟  马震原  王东方  王翠 《中国动物检疫》2018,35(12):85-90

为建立一种简便、快速、特异的猪伪狂犬病毒(PRV)gB抗原检测方法,利用单克隆抗体技术和侧向层析(LFA)技术,采用柠檬酸三钠还原法,制备颗粒大小为31.5 nm胶体金,再用胶体金标记纯化的抗PRV gB单克隆抗体10D4,在硝酸纤维素膜的质控带和检测带处,分别包被羊抗鼠IgG二抗和单克隆抗体6E9,组装成LFA方法通用胶体金层析试纸条。经测试,该试纸条最低能检测出1:640倍稀释的,TCID_(50)为1×10~(8.6)/0.1 mL的灭活PRV抗原,并与古典猪瘟病毒(CSFV)、猪繁殖与呼吸综合征病毒(PRRSV)、猪圆环病毒2型(PCV2)、猪细小病毒(PPV)、猪流行性腹泻病毒(PEDV)均无交叉反应,特异性良好;室温干燥密闭的条件下,该试纸条至少可保存10个月。结果表明,本研究建立的试纸条方法操作简单、快速、敏感、特异,易于判定,适合基层PRV的大面积普查和现场检测。  相似文献   

Dvl1及其突变体腺病毒载体的构建与在MSCs中表达鉴定     

王春燕  崔恒进  胡君  谢桂琴  张焕相 《广西农业生物科学》2014,(2):314-321

Dvl1及其结构缺失突变体ΔDIX（dsh and axin）和ΔDEP（dsh,Egl-10 and pleckstrin）腺病毒载体的构建并验证其在MSCs（mesenchymal stem cells）中的表达情况。将目的基因定向克隆至pAdTrack-CMV穿梭载体上,并经PmeⅠ线性化后在BJ5183细菌中与pAdEasy-1骨架质粒同源重组,获得重组腺病毒载体,在QBI-293A细胞中包装及扩增,实时荧光定量PCR和Western bolt验证Dvl1在MSCs中的表达情况。经PCR、PacⅠ单酶切鉴定及测序分析,成功构建Ad-Dvl1、Ad-ΔDIX和Ad-ΔDEP腺病毒载体,目的基因序列与GenBank报道一致,并选出150 MOI为最适感染MSCs的感染复数,成功构建了Ad-Dvl1、Ad-ΔDIX和Ad-ΔDEP腺病毒载体,并获得高滴度的病毒子,qPCR和Western blot证实Dvl1在MSCs中高表达并增加了β-catenin在细胞中的累积,为进一步研究Dvl1在MSCs迁移过程的作用奠定了基础。  相似文献   

Copper‐Induced Oxidative Stress and Responses of the Antioxidant System in Roots of Medicago sativa     

S.‐H. Wang  H. Zhang  Q. Zhang  G.‐M. Jin  S.‐J. Jiang  D. Jiang  Q.‐Y. He  Z.‐P. Li 《Journal of Agronomy and Crop Science》2011,197(6):418-429

The effects of various copper (Cu) concentrations on the antioxidative system in the roots of Medicago sativa were explored. The results indicated that the Cu content of the roots reached a value of 854 μg g^?1 DW at 10 μm Cu and a value of 4415 μg g^?1 DW at 100 μm Cu, suggesting that M. sativa has better ability to tolerate and accumulate Cu than other Cu‐bioaccumulators, and is a potential plant for phytoremediation. Treatment with Cu resulted in a significant increment in the levels of H₂O₂, O₂˙^? and OH˙. The reduced form of ascorbate and glutathione reached a peak at 30 μm Cu, and was followed by a sharp depletion to a lower level than that of the control. In contrast, the levels of the oxidised forms of ascorbate and glutathione showed a progressive increment with increasing Cu concentrations, suggesting that the antioxidant system was unable to cope with Cu stress at higher Cu levels. Under the Cu concentrations tested, the activity of catalase (CAT, EC 1.11.1.6), ascorbate peroxidase (APX, EC 1.11.1.11) and glutathione reductase (GR, EC 1.6.4.2) increased at lower Cu concentrations, and then decreased, reaching a maximum at 30 μm of Cu for APX and GR, at 10 μm for CAT, whereas the activities of guaiacol peroxidase (POD, EC 1.11.1.7) were gradually increased with increasing Cu concentrations. PAGE analysis of superoxide dismutase (SOD, EC 1.1.5.1.1) revealed that one band is a Mn‐SOD and five bands are identified as Cu, Zn‐SOD, whereas Fe‐SOD isoforms were not found in the roots of alfalfa. Cu at 10–100 μm increased the intensity of constitutive isozymes of CAT, APX and POD, whereas it decreased the intensity of isozymes of glucose‐6‐phosphate dehydrogenase (G6PDH, EC 1.1.1.49) significantly. The activities of lipoxygenases (LOX, EC 1.13.11.12) were gradually augmented with increasing Cu concentrations, demonstrating that LOXs are probably involved in production of lipid hydroperoxides and superoxide anion. There was a continuous and pronounced enhancement in the activity of esterase (EST, EC 3.1.1.1) in roots treated with 10–30 Cu μm , whereas EST activity in roots exposed to above 30 μm Cu declined, suggesting that EST plays a protective role under lower Cu concentrations stress.  相似文献   

谷子秸秆剪切力与其饲料营养特性变化规律及相互关系   总被引：1，自引：1，他引：0  

张燕青  崔清亮  辛林  李红波  来思彤  刘俊丽 《农业工程学报》2019,35(5):41-50

为探究谷子秸秆力学性质与其饲料特性的变化规律及相互关系，选用谷物完熟期的谷子秸秆，将其分为5段（近根部、中下部、中部、中上部、上部）并每隔7 d进行秸秆节间、茎节力学性质（剪切、压缩）和饲料特性（形态指标、营养成分）测定。试验结果表明：谷物完熟后，随着时间的推进，秸秆节间、茎节剪切力、抗压强度、干物质含量、纤维素含量、半纤维素含量和木质素含量总体上呈增大趋势，而秸秆含水率、当量直径、横截面积、线性密度和粗蛋白含量总体上呈减小趋势。谷子秸秆单位直径剪切力与含水率和线性密度呈负相关关系（R2≥0.903），与其干物质、纤维素、半纤维素和木质素含量呈正相关关系（R2≥0.845），与其粗蛋白含量无显著相关关系（P>0.05）。采收期内，谷子秸秆基部起秸秆剪切力至顶部逐渐增大，且剪切力与当量直径、横截面积呈正相关关系（R2≥0.916）。试验表明谷子秸秆饲料特性指标验证试验值与预测值相对误差不大于6.48%，预测模型合理。测量秸秆剪切力可用于预测其形态指标参数和营养成分含量，进而一定程度地反映秸秆饲用价值和反刍动物择食趋向。采收期内，谷子中部及以上秸秆单位直径剪切力显著小于其近根部（P<0.05），更适合饲用；且谷物完熟后，及时采收可避免秸秆饲用价值降低。该研究可为高效利用谷子秸秆资源提供参考。  相似文献   

5‐Aminolevulinic Acid Activates Antioxidative Defence System and Seedling Growth in Brassica napus L. under Water‐Deficit Stress     

D. Liu  Z. F. Pei  M. S. Naeem  D. F. Ming  H. B. Liu  F. Khan  W. J. Zhou 《Journal of Agronomy and Crop Science》2011,197(4):284-295

The present study assesses the effects of 5‐aminolevulinic acid (ALA, 0, 0.1, 1 and 10 mg l^?1) on the growth of oilseed rape (Brassica napus L. cv. ZS758) seedlings under water‐deficit stress induced by polyethylene glycol (PEG 6000, 0 and ?0.3 MPa). Water‐deficit stress imposed negative effects on seedling growth by reducing shoot biomass, cotyledon water potential, chlorophyll content and non‐enzymatic antioxidants (glutathione and ascorbic acid) levels. On the other hand, water‐deficit stress enhanced the malondialdehyde (MDA) content, reactive oxygen species (ROS) production, enzymatic antioxidants activities, reduced/oxidized glutathione ratio (GSH/GSSG) and reduced/oxidized ascorbic acid (ASA/DHA) ratio in seedlings. Application of ALA at lower dosages (0.1 and 1 mg l^?1) improved shoot weight and chlorophyll contents, and decreased MDA in rape seedlings, whereas moderately higher dosage of ALA (10 mg l^?1) hampered the growth. The study also indicated that 1 mg l^?1 ALA improved chlorophyll content, but reduced MDA content and ROS production significantly under water‐deficit stress. Lower dosages of ALA (0.1 and 1 mg l^?1) also enhanced GSH/GSSG and ASA/DHA as compared to the seedlings under water‐deficit stress. The antioxidant enzymes (ascorbate peroxidase, peroxidase, catalase, glutathione reductase and superoxide dismutase) enhanced their activities remarkably with 1 mg l^?1 ALA treatment under water‐deficit stress. It was also revealed that 1 mg l^?1 ALA treatment alone induced the expression of APX, CAT and GR substantially and under water‐deficit stress conditions ALA treatment could induce the expression of POD, CAT and GR to a certain degree. These results indicated that 0.1–1 mg l^?1 ALA could enhance the water‐deficit stress tolerance of oilseed seedlings through improving the biomass accumulation, maintaining a relative high ratio of GSH/GSSG and ASA/DHA, enhancing the activities of the specific antioxidant enzymes and inducing the expression of the specific antioxidant enzyme genes.  相似文献   

序贯疗法治疗圈养黑猩猩支原体肺炎一例     

刘金鹏  崔多英  张媛媛  普天春  杨明海  张成林  张金国 《野生动物》2011,32(4):221-223

肺炎支原体对于灵长类幼年黑猩猩的感染,与人类幼儿的临床表现和发病规律相似。该病例幼年黑猩猩早期症状偶见咳嗽,听诊肺部有湿性罗音,体温38.3℃。初步诊断为肺炎,用抗病毒药和抗生素头孢克洛治疗无效。随着病程发展,体温进一步升高到39℃以上,几乎无食欲。拍X光片和采血做支原体抗体检测,结果显示支原体感染强阳性。最终使用阿奇霉素采取＂停4喂3＂的序贯疗法治疗,即阿奇霉素输液3d——口服5d——停药4d——口服3d——停药4d——口服3d,其他则对症治疗。动物在治疗第7天时除偶见咳嗽,症状基本消除;治疗20d后,动物恢复健康。需要注意的是,治疗过程中一定要按照序贯疗法坚持用药治疗,防止疾病复发和减小用药的副作用。  相似文献   

饲粮代谢能水平对14～17周龄不同性别北京油鸡育肥期生长性能、屠宰性能、肉品质及血清生化指标的影响     

刘雄杰  耿爱莲  常枨  张尧  初芹  张剑  晏志勋  张小月  丁保安  刘华贵 《动物营养学报》2023,(4):2251-2263

本试验旨在研究饲粮代谢能水平对14～17周龄不同性别北京油鸡育肥期生长性能、屠宰性能、肉品质及血清生化指标的影响，为确定北京油鸡适宜饲粮代谢能水平提供依据。本试验严格按照完全随机区组试验设计进行。选取13周龄北京油鸡600只，公母各占1/2，根据体重一致原则随机分为6组，其中3个组均为公鸡，另外3个组均为母鸡，每组5个重复，每个重复20只。3个组分别为低代谢能组（饲粮代谢能水平为12.20 MJ/kg）、中代谢能组（饲粮代谢能水平为12.64 MJ/kg）、高代谢能组（饲粮代谢能水平为13.08 MJ/kg）。预试期1周，正试期4周（14～17周龄）。结果显示：1）高代谢能组公鸡和母鸡的平均日增重显著高于其他2组（P <0.05），且高代谢能组母鸡的料重比显著低于其他2组（P <0.05），饲粮代谢能水平为13.08 MJ/kg时公鸡和母鸡有较好的生长性能。2）公鸡方面，低代谢能组的腿肌率显著高于其他2组（P<0.05）；母鸡方面，低代谢能组的全净膛率显著高于高代谢能组（P<0.05），翅膀率显著低于高代谢能组（P <0.05）。低代谢能组公鸡和母鸡的皮脂...  相似文献   

油罐内浮盘密封失效原因与预防措施     

聂世全  崔振兴  王伟峰 《油气储运》2011,30(6):468-471,391,392

针对油罐内浮盘密封失效问题逐渐凸显的现状,分析了内浮盘静、动密封与油气空间的关系,总结了油罐内浮盘密封失效的主要表现,静密封失效包括:内浮盘盖板搭接面间出现缝隙孔洞,密封胶囊或舌形密封带与内浮盘外边缘间的挟紧部位出现缝隙,防旋导管与内浮盘盖板间不密封,浮盘自动通气孔、人孔与盖板间不密封;动密封失效包括:密封胶囊或舌形密封带与罐内壁间密封性能差,量油导管与量油导管护筒盖间的密封性能差,浮盘人孔盖与人孔座间的密封性能差,防旋转钢丝绳松紧度不满足浮盘安全运行要求。基于设计、施工、使用、检修4个环节分析归纳了内浮盘密封失效的原因,提出了优化设计、控制施工质量、控制内浮盘制造安装质量、内浮盘定期检测与管理、规范检修作业等预防措施。  相似文献