New Zealand caprine herpes virus: comparison with an Australian isolate and with bovine herpes virus type 1 by restriction endonuclease analysis     

Tisdall DJ  Bentley CB  Collins DM  Horner GW 《New Zealand veterinary journal》1984,32(6):99-100

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Levels of selenium in blood and tissues associated with some selenium deficiency diseases in New Zealand sheep     

Sheppard AD  Blom L  Grant AB 《New Zealand veterinary journal》1984,32(6):91-95

Whole bloods from weaned lambs with severe selenium responsive unthriftiness usually contain <5 ng Se/ml. Mildly or moderately affected lambs have blood levels of 5-10 ng/ml. Selenium responsive infertility in ewes appears to be associated with whole blood selenium levels below 10 ng/ml.  相似文献   

Artificial Intelligence in Parallel: Working with many processors at once could accelerate computation enormously--and suggest new ways to think about thinking     

Waldrop MM 《Science (New York, N.Y.)》1984,225(4662):608-610

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Comparative studies of strains of infectious bovine rhinotracheitis virus isolated from latently infected calves     

G Castrucci  F Frigeri  S Ranucci  M Ferrari  V Cilli  B Pedini  P Nettleton  F Caleffi  V Aldrovandi  A J Herring 《Comparative immunology, microbiology and infectious diseases》1984,7(1):1-10

Three strains (479 C, 778 TL, 982 LE) of infectious bovine rhinotracheitis (IBR) virus isolated from latently infected calves were compared with the prototype strain of IBR virus (LA strain) in studies which included restriction endonuclease analysis, experimental infection, and reciprocal cross protection tests in cattle. From the restriction endonuclease analysis it appeared that the 3 "latent" viruses were derived from the same isolate, and that it differed slightly from the LA strain. However, latency does not seem to have affected the pathogenicity or the immunogenicity of the virus. This is demonstrated by the identical clinical and virologic response of calves subjected to experimental infection with the various strains under study, and by the finding that when the LA strain and a "latent" strain (982 LE) were tested in cross protection tests in cattle, they proved to be mutually protective.  相似文献   

Specificities of antinuclear antibodies detected in dogs with systemic lupus erythematosus   总被引：4，自引：0，他引：4  

O Costa  C Fournel  E Lotchouang  J C Monier  M Fontaine 《Veterinary immunology and immunopathology》1984,7(3-4):369-382

Five hundred and eighty dogs with at least one clinical sign compatible with a systemic lupus erythematosus (SLE) were entered in a prospective study aimed at evaluating the prevalence of antinuclear antibodies (ANAb). SLE was diagnosed in 38 of these dogs (group A) which fulfilled at least four American Rheumatism Association (ARA) criteria; of these, sixteen had ANAb titers greater than or equal to 4096. The 23 dogs which met three or two ARA criteria (group B) had an ANAb geometric mean titer (GMT) of 259. Dogs (group C) with only 1 criterium had an ANAb GMT of 75. Anti-ds-DNA Ab were present in 6 dogs from group A (16%), and 2 dogs from group B (9%). Anti-histone Ab were present among dogs from group A, B and C with frequencies of 81%, 67% and 26%, respectively. Among dogs from group A, the ANAb titers and the levels of anti-histone Ab correlated positively when individual sera were considered. Antibodies against the soluble nuclear antigen (SNA) were detected in 74%, 39% and 13% of the dogs from groups A, B and C, respectively. Antibodies initially described in human SLE also exist in SLE dogs. Anti-Sm Ab were found in 24% of dogs in group A. With anti-RNP Ab the frequency was still lower (10%). However, two other types of anti-SNA Ab against RNAse and trypsin-resistant antigens, not found in human "reference sera", were often detected. The first type (anti-type 1 Ab) was found in 26% and 9% of group A and group B, The first type (anti-type 1 Ab) was found in 26% and 9% of group A and group B, respectively; the second type (anti-type 2 Ab) is less frequent, and was found in 13% and 17% of group A and B, respectively. It appears that testing for anti-Sm, anti-type 1 and anti-histone Ab should be performed in order to improve the diagnosis of SLE in dogs.  相似文献   

An automated micro-method for the enzymatic determination of D-B-hydroxybutyrate in ruminant plasma     

D. P. Knox  D. G. Jones 《Veterinary research communications》1984,8(1):103-115

1. An enzymatic ‘reaction rate’ micro-method for the rapid routine estimation of D-B-hydroxybutyrate (D-B-HOB) in ruminant plasma, using an I.L. Multistat III centrifugal analyzer, is described.
2. Reaction conditions were optimized to give a linear response for plasma D-B-HOB concentrations between 100 and 2500 μmoles per litre, at 30°C and pH 9.0.
3. For the standardized method the within-run and between-run coefficients of variation for deproteinised ovine plasma were consistently less than 3.5%.
4. There was good agreement between plasma concentrations obtained by the present method and both original U.V. end-point technique (r=0.927b=0.950) and a colorimetric end-point procedure (r=0.937. b=0.879).
5. Utreated ovine and bovine plasma consistently exhibited high ‘blank’ activity and this was directly correlated with plasma lactate dehydrogenase (LDH) activity in both species (r=0.971; p<0.001 and r=0.949; p<0.001 respectively). The distribution of LDH activity in man was similar to sheep but, contrastingly, non-specific interference was extremely low in human plasma and unrelated to LDH. Horse, chicken and rat had negligible ‘blank” activity and comparatively low LDH levels. In both cattle and sheep non-specific interference was abolished by perchloric acid precipitation. In the sheep subtraction of ‘blank’ activity gave D-H-HOB concentrations for untreated plasma comparable to those in deproteinised samples. However, in the bovine, D-B-HOB levels remained significantly (t=6.44; p<0.001) higher even after ‘blank’ correlation. In contrast to man and other non-ruminants, perchloric acid precipitation is essential in ruminants to avoid false overestimation of plasma D-B-HOB levels.
6. Plasma with EDTA as anticoagulant and serum gave concentrations of D-B-HOB approximately 60% lower, than samples containing heparin or oxalate/fluoride. However, heparin was associated with much higher (up to 50%) non-specific NAD rduction than oxalate/fluoride.
7. High levels of acetoacetate (400–1000 μmoles per litre) reduced the recovery of D-B-HOB from ovine plasma by less than 10%. This effect was negated by the inclusion of hydrazine hydrates in the reaction mixture. Perchlorate ion concentrations above 25 μmoles per litre per test dramatically inhibited the assay in ovine plasma, and therefore precipitation conditions must be carefully controlled.
8. Plasma with oxalate/fluoride as anticoagulant showed the greatest stability in storage; 24 hours at room temperature, one week at +4°C and at least one month at ?20°C.

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The overwintering mode ofBemisia tabaci and its parasitoids in Israel     

D. Gerling 《Phytoparasitica》1984,12(2):109-118

Wild and cultivated plants in the vicinity of Kibbutz Nahshon and a few additional locations in Israel were sampled for the presence ofBemisia tabaci Genna-dius (Homoptera: Aleyrodidae). The whiteflies, together with their parasites,Eretmocerus mundus andEncarsia lutea, were found to develop on numerous host species throughout the winter. Especially high levels were reached onLan-tana camara, Abutilon grandifolium andIpomoea batatas. During late winter and spring the population on these hosts declined. From April onwards the populations increased on potatoes and sunflowers.  相似文献   

The hymenopterous parasites of Nitidulidae     

Roger N. Williams  Michael J. Weiss  M. Kehat  D. Blumberg 《Phytoparasitica》1984,12(1):53-64

The known information on hymenopterous parasites of Nitidulidae was consolidated in this article. Species of parasites, nitidulid hosts and, where possible, plant host of the nitidulids are provided along with citations to sources of information. Thirty-three species of hymenopterous parasites are recorded in nine different families. Since nomenclature of several of the parasites has changed, it was updated with the assistance of specialists.  相似文献   

Detection of beet yellows virus in sugarbeet plants by enzyme-linked immunosorbent assay (ELISA)     

P. H. M. Roseboom  D. Peters 《European journal of plant pathology / European Foundation for Plant Pathology》1984,90(4):133-141

Beet yellows virus can be detected in leaf extracts of infected sugarbeet plants by ELISA. The use of discs was studied and proved to be a valuable and qualitatively reliable method. Leaf material could be stored at 4^o or 22°C for at least six days without affecting the detection of this virus by ELISA. A dramatic decrease in ELISA values was found when leaf extracts were frozen.In an analysis of the distribution of virus over the plant it was found that young leaves present at the moment of infection and those which had still to develop after infection will contain virus. Symptoms produced by systemic virus invasion occur on the oldest leaves containing virus.Samenvatting Het bietevergelingsvirus kan op betrouwbare wijze met de ELISA methode in geïnfecteerde bieteplanten worden aangetoond. Een aanzienlijke vereenvoudiging van de procedure kan worden bereikt met de zogenaamde disc-method, waarbij intacte ponsstukjes in de putjes van de ELISA-plaat worden geïncubeerd. Hierbij komt voldoende virus uit de ponsstukjes voor ELISA vrij. Bladmateriaal kon op verschillende wijzen bewaard worden zonder dat de mogelijkheid om het virus aan te tonen achteruitging. Met bladextracten die ingevroren waren, werden echter slechte resultaten verkregen.In een analyse naar de verdeling van het virus over het loof bleek het virus voor te komen in de geïnoculeerde bladeren, in die bladeren die op het tijdstip van inoculatie minder dan de helft van hun uiteindelijke lengte bereikt hadden en in de bladeren die nog moesten verschijnen. De symptomen ontwikkelden zich op de oudste systemisch geïnfecteerde bladeren.  相似文献   

Continuing education in immunology. I: the structure and function of immunoglobulins     

Moriarty KM 《New Zealand veterinary journal》1984,32(5):61-64

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