Different Culture Media Requirements of IVF and Nuclear Transfer Bovine Embryos   总被引：3，自引：0，他引：3  

GF Mastromonaco  E Semple  C Robert  GJ Rho  DH Betts  WA King 《Reproduction in domestic animals》2004,39(6):462-467

Important differences exist between in vitro fertilized (IVF) and nuclear transfer (NT) bovine embryos. Studies have shown that although in vitro development is comparable, post-implantation survival is greatly reduced in NT embryos. In this study, we compare serum and bovine serum albumin (BSA) supplementation during oocyte maturation and embryo culture of IVF and NT embryos. In experiment 1, oocytes and embryos were randomly distributed into different treatment groups consisting of synthetic oviductal fluid (SOF) medium supplemented with either serum, fatty acid-free BSA (FAF) or fraction V BSA during maturation and/or culture to assess IVF embryo development. In experiment 2, oocytes were matured in SOF + serum or SOF + FAF and reconstructed embryos were cultured in SOF + FAF to assess NT embryo development. Among the IVF treatment groups, a greater number of blastocysts were observed in the steer serum (SER) group (IVM and IVC in SOF + serum) on day 6; however, no significant differences were seen in blastocyst development from day 8 onwards. Hatching frequencies on days 8 and 9 were significantly greater in groups with serum, with the exception of FAF (IVM and IVC in SOF + FAF) on day 9. For the NT treatment groups, the presence of serum during IVM resulted in a higher proportion of MII oocytes and increased blastocyst development and hatching rates were compared with supplementation of FAF. These results indicate that both serum and FAF provide comparable embryo development for IVF but not for NT bovine embryos.  相似文献   

Subcutaneous granuloma caused by Mycobacterium avium complex infection in a cat     

R. MALIK  L. GABOR  P. MARTIN  DH MITCHELL  DJ DAWSON 《Australian veterinary journal》1998,76(9):604-607

A localised subcutaneous swelling developed on the nasal bridge of a cat receiving chemotherapy for alimentary tract lymphosarcoma. Cytological and histological examination of representative samples of the lesion demonstrated pyogranulomatous inflammation and abundant acid-fast bacilli. A Mycobacterium sp was cultured from tissue excised from the lesion. Extensive testing at three reference laboratories indicated the strain was a member of the Mycobacterium avium complex. The infection was treated successfully by cytoreductive surgery and a 6 weeks course of orally administered clofazimine.  相似文献   

Asymmetric scattering of polarized electrons by organized organic films of chiral molecules     

K Ray  SP Ananthavel  DH Waldeck  R Naaman 《Science (New York, N.Y.)》1999,283(5403):814-816

Electron transmission experiments demonstrate a large asymmetry in the scattering probability of polarized electrons by thin organized films of chiral molecules. This large asymmetry results from the interaction of the electron's wavefunction with many scatterers (molecules) in the organized monolayer structure and represents a manifestation of quantum interference on the scale of supramolecular lengths.  相似文献   

The Effect of Prostaglandin E₂ on the Cervices of Dioestrous and Prepartum Mares     

DH Volkmann  HJ Bertschinger  ML Schulman 《Reproduction in domestic animals》1995,30(4):240-244

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Veterinary education in the European Community     

A O Betts 《The Veterinary record》1991,128(20):479-480

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Blindness in a dog with IgA-forming myeloma     

S E Kirschner  Y Niyo  B L Hill  D M Betts 《Journal of the American Veterinary Medical Association》1988,193(3):349-350

A 9-year-old dog with a 2-month history of weight loss and a 1-week history of blindness had an IgA-forming myeloma. Seemingly, the blindness was a result of bilaterally detached retinas. The dog also had leukopenia, anemia, hypoalbuminemia, hyperglobulinemia, and proteinuria as well as lytic lesions in the cervical portion of the spine and high IgA concentrations in serum and urine. Evaluation of aspirates from the subretinal spaces revealed lymphocytes in a proteinaceous fluid. Histologically, retinal lesions consisted of vascular endothelial cell damage and intraretinal cysts and hemorrhages.  相似文献   

Future of RVC's Camden Town site     

A O Betts 《The Veterinary record》1987,121(11):262-263

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Ocular manifestations of a metastatic pulmonary adenocarcinoma in a cat   总被引：1，自引：0，他引：1  

J R Gionfriddo  A S Fix  Y Niyo  L D Miller  D M Betts 《Journal of the American Veterinary Medical Association》1990,197(3):372-374

A primary pulmonary bronchogenic adenocarcinoma originating from an intramediastinal accessory lung was diagnosed in a 14.5-year-old cat. The cat had been admitted because of a cloudy right eye. Physical examination revealed a thin cat with severe iritis, aqueous flare, and a fibrin clot in the anterior chamber of the right eye. Right fundic examination revealed bullous retinal detachment superior to the optic disc. Euthanasia and necropsy were requested when FeLV test results were positive. Metastatic neoplastic cells similar to those of the primary tumor were detected in the choroid, ciliary body, and ciliary processes of the right eye. Ciliary and iridic stromal necrosis attributable to neoplastic embolization of uveal vessels had led to severe uveitis. Foci of metastasis were also in the heart, kidney, and cerebral meninges.  相似文献   

The experimental disease produced in calves by the J-121 strain of parainfluenza virus type 3   总被引：4，自引：0，他引：4  

A R Omar  A R Jennings  A O Betts 《Research in veterinary science》1966,7(4):379-388

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S. P. F. animals     

A O Betts 《Wiener tier?rztliche Monatsschrift》1965,52(5):505-516

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