Enhanced growth and immuno‐physiological response of Genetically Improved Farmed Tilapia in indoor biofloc units at different stocking densities          下载免费PDF全文

Harsha Haridas  Ajit Kumar Verma  Gaurav Rathore  Chandra Prakash  Paramita Banerjee Sawant  Asanaru Majeedkutty Babitha Rani 《Aquaculture Research》2017,48(8):4346-4355

An experimental trial was conducted for 90 days to evaluate the growth performance, immunophysiological response of GIFT strain of Tilapia in biofloc‐based rearing system and to assess the relative percentage survival in 3 days after challenging with the virulent strain of Aeromonas hydrophila. Fingerlings with an average body weight 0.98 ± 0.06 g were stocked in triplicate at different stocking densities of 200 (SD1), 250 (SD2), 300 (SD3) and 350 (SD4) m^?3 in biofloc‐based treatments and 150 (C) m^?3 in control (clear water). Biofloc‐based units (SD1 and SD2) obtained significantly better (P < 0.05) growth performances at the end of the experimental period. Mean body weight of fish in biofloc‐based units showed a decreasing trend with increase in stocking density with 100% survival in all units including control. The stress parameters were significantly lower in biofloc‐based rearing units especially in treatments SD1 and SD2 as compared to the control. The fish from the biofloc‐based units (SD1 and SD2) possessed significantly (P < 0.05) higher immune status as compared to control and other biofloc treatments in terms of respiratory burst, serum lysozyme and myeloperoxidase activity. Relative survival percentages were significantly better in biofloc treatments with highest in SD1 and SD2 (83.33%) after challenge study. GIFT strain of Tilapia at higher stocking densities 200–250 nos m^?3 can be taken as optimum stocking density whereas higher stocking densities up to 350 nos m^?3 can be reared in the biofloc systems without compromising the growth and immunity.  相似文献   

Role of sulfur nutrition in plant and seed metabolism of Glycine max L.     

Neelam Chandra  Nalini Pandey 《Journal of plant nutrition》2016,39(8):1103-1111

The aim of present investigation has been to explore the effect of sulfur application on plant metabolism, seed yield and seed quality in soybean. The sulfur was supplied in different doses ranging as 1, 2, 4, 6 and 8 meq S L^?1. Plant supplied with 4 meq S L^?1 showed optimal growth. Plant growth and dry matter was reduced under sulfur deficiency (1 and 2 meq S L^?1) and toxicity (6 and 8 meq S L^?1). Application of sulfur increases the tissue sulfur and cysteine concentration in both leaves and seeds. The critical concentration for deficiency (CCD) and toxicity (CCT) of sulfur was observed 0.194 to 0.277% dry weight respectively. Pod yield and seed yield was also suppressed in sulfur deficiency and toxicity. In leaves sugar (reducing, non-reducing and total sugar) and starch was found to be accumulated while in seeds both were depleted under sulfur deficiency and toxicty. Seed storage proteins (albumins, globulins, glutelins and prolamins) were also reduced under sulfur stress. Thus, we conclude that sulfur deficiency and toxicity both affects the plant metabolism, yield and seed quality in terms of carbohydrates and storage proteins of soybean.  相似文献   

Influence of leguminous cover crops on microbial and selected enzyme activities in soils of a plantation     

R. Dinesh  M. A. Suryanarayana  G. Shyam Prasad  A. K. Bandyopadhyay  A. K. Nair  T. V. R. S. Sharma 《植物养料与土壤学杂志》1999,162(1):57-60

A study dealing with ecological sustainability of plantation based land use initiated in 1991 in a 19 yr old coconut plantation consists of growing certain leguminous crops like Atylosia, Pueraria, Centrosema and Calopogonium as soil cover in separate plots with lemon grass as live bounds. These cover crops are grown during the rainy season and incorporated into the soil towards the end of the monsoon every year. The effect of such cover crops on soil microbial counts (total counts, fungi, actinomycetes and bacteria), biomass C, organic C, total N and on the activity of enzymes like urease, amidase, L-glutaminase, aryl sulphatase and dehydrogenase was determined in soils (Ap horizon) collected from these plots after 5 years. Soils with cover crops registered significantly higher microbial biomass, biomass C, organic C and total N compared to control. Consequently, all the enzymes were activated to different degrees in soils with cover crops. Significant and positive relationships of enzyme activities with organic C, mineral N and total N suggested that growing cover crops, increased C turnover and N availability and therefore, provided a conducive environment for microbial proliferation, enzyme synthesis and accumulation in the soil matrix.  相似文献   

Quantitative trait loci for stay-greenness and agronomic traits provide new insights into chlorophyll homeostasis and nitrogen use in rice     

Ramakrishnappa Archana  Kunnummal Kurungara Vinod  Subbaiyan Gopala Krishnan  Elangovan Devi Chandra Vadhana  Prolay Kumar Bhowmick  Vikram Jeet Singh  Ranjith Kumar Ellur  Lekshmy Sathee  Pranab Kumar Mandal  Haritha Bollinedi  Shekharappa Nanda Kumar  Sonu  Mariappan Nagarajan  Ashok Kumar Singh 《Plant Breeding》2023,142(3):312-326

Functional stay-green (FSG) is characterized by delayed senescence during the reproductive stage in rice. A recombinant inbred line (RIL) population derived from ‘Pusa 677’/‘PSG16’ was used to study the genetics of FSG in a rice mutant, ‘PSG16’. The RILs exhibited significant variation under two N regimes for agro-morphological traits, leaf chlorophyll content, flowering time, yield components and nitrogen (N) use. Using a genome wide linkage map spanning 1910.8 cM with 104 polymorphic markers, we have mapped six major quantitative trait loci (QTLs). One robust QTL on chromosome 1 was associated consistently across different N regimes with chlorophyll content and flowering time. The QTL on chromosome 7 was associated with grain number, whereas the QTL on chromosome 6 was found related to N harvest index and spikelet fertility. Although ‘PSG16’ showed a clear advantage in grain yield as well as having better N assimilation, we could not establish a direct genetic relationship SG trait and N use. Based on the QTL information, FSG trait of ‘PSG16’ could be useful in deciphering multiple stress responses in rice.  相似文献   

Odontogenic myxoma of the mandible in a filly.     

A M Chandra  C D Buergelt  M T Ethell 《Journal of veterinary diagnostic investigation》1999,11(3):274-277

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Soil Microbial Biomass and Enzyme Activities as Influenced by Organic Manure Incorporation into Soils of a Rice-Rice System   总被引：1，自引：0，他引：1  

R. Dinesh  R. P. Dubey  G. Shyam Prasad 《Journal of Agronomy and Crop Science》1998,181(3):173-178

A fertilizer management study involving incorporation of poultry manure, farm yard manure, sesbania and gliricidia into soils of a rice-rice system was initiated in May 1993. In order to determine the effects of organic manure incorporation on soil microbial biomass and enzyme activity, soils were collected from the respective plots at the end of the second rice crop in February 1996. and were incubated with and without the respective organic manure at the rate of 15 Mg ha⁻¹ at 25°C, under submergence. The total viable microbial counts, bacteria, actinomycetes, N flush, biomass C and activities of urease, amidase, acid and alkaline phosphatase, dehydrogenase and L-glutaminase were determined after 60 days of incubation.
Soils freshly amended and soils previously amended with organic manures registered significantly greater microbial biomass and enzyme activity than the unamended control. The microbial biomass and enzyme activity, however, varied with the type of organic manure incorporated into the soil. Except for acid phosphatase, which showed slight inhibition, all the other enzymes were activated to different degrees by organic manure incorporation. A significant and positive relationship of enzyme activity with organic C and total N suggested that the addition of organic manure to soils increased C turnover, N availability and microbial activity which in turn led to greater enzyme synthesis and accumulation in the soil matrix.  相似文献   

Effect of Water Stress on Biochemical and Physiological Characteristics of Oat Genotypes     

A. Chandra  R. K. Bhatt  L. P. Misra 《Journal of Agronomy and Crop Science》1998,181(1):45-48

Activities of enzymes catalase, peroxidase, and level of total soluble proteins were studied in two genotypes (JHO 884, JHO 888) of oat ( Avena sativa L.) subjected to water stress for 1, 3, and 4 days by withholding water in a pot culture experiment. No change was observed in the level of total soluble proteins at early stage but it increased significantly at higher magnitude of water stress. Increase in catalase activity was noticed in JHO 884 whereas reverse trend was observed in JHO 888 at highest level of stress. The JHO 884 genotype showed increase in peroxidase activity from the first day of water stress. Results suggest that the efficiency level of antioxidant system is found different in these two genotypes.  相似文献   

Water productivity and nutrient status of rice soil in response to cultivation techniques and nitrogen fertilization     

Soumya Saha  Y. V. Singh  Sunita Gaind  Dinesh Kumar 《Paddy and Water Environment》2015,13(4):443-453

Three methods of rice cultivation were compared in a field experiment at New Delhi, India during 2012 for their water use and changes in nutrient availability of soil. The experiment was laid out in a split plot design with conventional transplanting (CT), system of rice intensification (SRI), and aerobic rice (AR) cultivation technologies. Five doses of nitrogen included 100 % (120 kg N ha^?1), 125, and 150 % recommended dose of N(RDN) through urea, 75 % of RDN through urea (90 kg N ha^?1) + 25 % of RDN (30 kg ha^?1) through farm yard manure (FYM), and 100 % of RDN through FYM. Results revealed that status of available N in soil under rice at 45 and 90 days after sowing (DAS) was significantly higher in CT and SRI compared to AR method. Application of the highest dose of nitrogen through urea resulted in the highest availability of N (188.9, 174.2, and 135.2 kg ha^?1 for 45 and 90 DAS and at harvest stage, respectively). The soil under AR recorded significantly low availability of phosphorus and iron. However, availability of K in soil was not affected significantly under adopted production techniques and nitrogen management. The recorded irrigation water productivity was maximum in AR cultivation (9.16 kg ha mm^?1) followed by SRI (7.02 kg ha mm^?1) with irrigation water saving of 54 and 36 %, respectively compared to CT.  相似文献   

Dietary microbial levan ameliorates stress and augments immunity in Cyprinus carpio fry (Linnaeus, 1758) exposed to sublethal toxicity of fipronil     

Sanjay Kumar Gupta  Asim Kumar Pal  Narottam Prasad Sahu  Neelam Saharan  Sagar Charan Mandal  Chandra Prakash  Mohammed Shahbaz Akhtar  Ashisa Kumar Prusty 《Aquaculture Research》2014,45(5):893-906

A 45‐day feeding trial was conducted to study the stress ameliorating and immunomodulatory role of microbial levan in Cyprinus carpio fry exposed to sublethal dose (1/10th LC₅₀) of fipronil [(±)‐5‐amino‐1‐(2,6‐dichloro‐α,α,α‐trifluoro‐p‐tolyl)‐4‐trifluoromethylsulfinylpyrazole‐3‐carbonitrile]. Two hundred and twenty‐five fry were randomly distributed in five treatments in triplicates. Four purified diets were prepared with graded levels of microbial levan. Five different treatment groups were levan control L₀P₀ (basalfeed + 0% levan without exposure to pesticide); pesticide control L₀P₁ (basalfeed + 0% levan with exposure to pesticide); L_0.25P₁ (basalfeed + 0.25% levan with exposure to pesticide); L_0.50P₁ (basalfeed + 0.50% levan with exposure to pesticide) and L_0.75P₁ (basalfeed + 0.75% levan with exposure to pesticide). Lactate dehydrogenase (LDH), malate dehydrogenase (MDH) and fructose‐1,6‐diphosphatase (FDPase) activites were significantly (P < 0.05) increased, whereas alkaline phosphatase (ALP), adenosine triphosphatase (ATPase) and acetyl choline esterase (AchE) activities were significantly (P < 0.05) reduced in higher levan‐fed groups. RBC, haemoglobin and WBC counts were significantly (P < 0.05) increased in the levan‐fed groups. Similar trends were also observed for the total serum protein, globulin, NBT and lysozyme activities. Blood glucose and serum cortisol exhibited a third order polynomial relationship with increasing level of dietary levan. Overall result showed stress ameliorating, immunostimulating and protective role of microbial levan against fipronil‐induced stress in C. carpio fry at 0.75% level of dietary levan supplementation.  相似文献   

Effect of water flow rate on polyculture of koi carp (Cyprinus carpio var. koi) and goldfish (Carassius auratus) with water spinach (Ipomoea aquatica) in recirculating aquaponic system     

K. K. T. Nuwansi  A. K. Verma  Chandra Prakash  V. K. Tiwari  M. H. Chandrakant  A. P. Shete  G. P. W. A. Prabhath 《Aquaculture International》2016,24(1):385-393

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