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排序方式: 共有58条查询结果,搜索用时 843 毫秒
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Evidence of the widespread occurrence of reticuloendotheliosis virus (REV) sequence insertions in fowl poxvirus (FPV) genome of field isolates and vaccine strains has increased in recent years. However, only those strains carrying a near intact REV provirus are more likely to cause problems in the field. Detection of the intact provirus or REV protein expression from FPV stocks has proven to be technically difficult. The objective of the present study was to evaluate current and newly developed REV and FPV polymerase chain reaction (PCR) assays to detect the presence of REV provirus in FPV samples. The second objective was to characterize REV insertions among recent "variant" FPV field isolates and vaccine strains. With REV, FPV, and heterologous REV-FPV primers, five FPV field isolates and four commercial vaccines were analyzed by PCR and nucleotide sequence analysis. Intact and truncated REV 5' long terminal repeat (LTR) sequences were detected in all FPV field isolates and vaccine strains, indicating heterogeneous REV genome populations. However only truncated 3' LTR and envelope sequences were detected among field isolates and in one vaccine strain. Amplifications of the REV envelope and 3' LTR provided strong evidence to indicate that these isolates carry a near intact REV genome. Three of the four FPV vaccine strains analyzed carried a solo complete or truncated 5' LTR sequence, indicating that intact REV provirus was not present. Comparison of PCR assays indicated that assays amplifying REV envelope and REV 3' LTR sequences provided a more accurate assessment of REV provirus than PCR assays that amplify the REV 5' LTR region. Therefore, to differentiate FPV strains that carry intact REV provirus from those that carry solo 5' LTR sequences, positive PCR results with primers that amplify the 5' LTR should be confirmed with more specific PCR assays, such as the envelope, or the REV 3' LTR PCR. 相似文献
3.
Roy Arpita Sharma Neelam Bharadvaja Navneeta 《Genetic Resources and Crop Evolution》2022,69(1):209-219
Genetic Resources and Crop Evolution - Selection of elite accessions is important to get the maximum quantity of bioactive compounds in medicinal plants. In this study, thirteen accessions... 相似文献
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A.K. Prusty M.P.S. KohliN.P. Sahu A.K. PalN. Saharan S. MohapatraS.K. Gupta 《Pesticide biochemistry and physiology》2011,100(2):124-129
Experiment was carried out to determine the median lethal concentration (LC50) of fenvalerate to Labeo rohita fingerlings. After determining the LC50 value of fenvalerate, a sub-lethal concentration (1/3rd of LC50) of fenvalerate was exposed for 15 days. Significant alterations in SOD (P < 0.05) activity of liver and gill was observed due to fenvalerate. Catalase activity in gills of fishes was also affected significantly (P < 0.05). WBC, NBT and Hct values were reduced significantly in fenvalerate exposed fishes as compared to control group, whereas blood glucose level showed higher values in fenvalerate exposed group. Serum total protein and albumin were also reduced significantly as a result of fenvalerate exposure. Significant increase in the serum GOT, serum GPT, creatinine, triglyceride and serum ACP was noticed after 15 days of fenvalerate exposure. Results indicated that short term exposure of fenvalerate can induce biochemical and haematological alterations causing stress to L. rohita fingerlings. 相似文献
6.
Drought tolerant and susceptible cultivars of wheat, C-306 and Kalyan sona, growing under non-stressed and water-stressed conditions, were sprayed with benzyladenine (BA) at 70 days after sowing (DAS). Observations recorded at 5, 10, 15, 20 and 25 days after spraying revealed that BA increased the rate of transpiration (TR) in C-306 under non-stressed conditions. However, under water stress, the increase was significant only after 5 days of BA spraying. In Kalyan sona BA treatment either decreased TR or did not exhibit significant increase under non-stressed conditions, but caused significant increase in TR under water stress. The increase in TR was elicited through enhanced stomatal opening. Water potential, osmotic potential and pressure potential of both genotypes decreased on account of water stress. The effect of BA was not perceptible on restoration of leaf water potential (LWP) or its components. The effect of BA was possibly confined to stomatal behaviour and transpiration. 相似文献
7.
‘Ladakhi tea’ <Emphasis Type="Italic">Bidens pilosa</Emphasis> L. (Asteraceae): a cultivated species in the cold desert of Ladakh Himalaya,India 总被引:1,自引:1,他引:0
Bidens pilosa L., a less-known cultivated tea species was collected from cold desert of Ladakh Himalaya, Jammu and Kashmir, India during
2004. The freshly harvested leaves are used in preparation of ‘Ladakhi tea’ locally known as ‘Saja’ or ‘Soljaa’ in cold desert
of Ladakh Himalaya, Western Himalaya, Jammu and Kashmir. The local inhabitants were growing this species in their kitchen
garden for this use. In the present communication, the method of preparation of this beverage is also discussed. 相似文献
8.
Nidhi Rawat Vijay K. Tiwari Neelam Singh Gursharn S. Randhawa Kuldeep Singh Parveen Chhuneja Harcharan S. Dhaliwal 《Genetic Resources and Crop Evolution》2009,56(1):53-64
Grains of 80 accessions of nine species of wild Triticum and Aegilops along with 15 semi-dwarf cultivars of bread and durum wheat grown over 2 years at Indian Institute of Technology, Roorkee,
were analyzed for grain iron and zinc content. The bread and durum cultivars had very low content and little variability for
both of these micronutrients. The related non-progenitor wild species with S, U and M genomes showed up to 3–4 folds higher
iron and zinc content in their grains as compared to bread and durum wheat. For confirmation, two Ae. kotschyi Boiss. accessions were analyzed after ashing and were found to have more than 30% higher grain ash content than the wheat
cultivars containing more than 75% higher iron and 60% higher zinc than that of wheat. There were highly significant differences
for iron and zinc contents among various cultivars and wild relatives over both the years with very high broad sense heritability.
There was a significantly high positive correlation between flag leaf iron and grain iron (r = 0.82) and flag leaf zinc and grain zinc (r = 0.92) content of the selected donors suggesting that the leaf analysis could be used for early selection for high iron
and zinc content. ‘Chinese Spring’ (Ph
I
) was used for inducing homoeologous chromosome pairing between Aegilops and wheat genomes and transferring these useful traits from the wild species to the elite wheat cultivars. A majority of
the interspecific hybrids had higher leaf iron and zinc content than their wheat parents and equivalent or higher content
than their Aegilops parents suggesting that the parental Aegilops donors possess a more efficient system for uptake and translocation of the micronutrients which could ultimately be utilized
for wheat grain biofortification. Partially fertile to sterile BC1 derivatives with variable chromosomes of Aegilops species had also higher leaf iron and zinc content confirming the possibility of transfer of required variability. Some of
the fertile BC1F3 and BC2F2 derivatives had as high grain ash and grain ash iron and zinc content as that of the donor Aegilops parent. Further work on backcrossing, selfing, selection of fertile derivatives, leaf and grain analyses for iron and zinc
for developing biofortified bread and durum wheat cultivars is in progress.
Nidhi Rawat, Vijay K. Tiwari, and Neelam Singh have contributed equally to the work. 相似文献
9.
Narayan D Chaurasiya Rajender S Sangwan Laxmi N. Misra Rakesh Tuli Neelam S Sangwan 《Fitoterapia》2009,80(8):496-505
Withania somnifera is one of the most important medicinal plants of Ayurveda and finds extensive uses in Indian traditional herbal preparations. In this investigation, selected accessions of the plant were examined for diversity through RAPDs, isoenzymes, polypeptide polymorphism and withanolide profiles. The accessions clustered together with respect to their characteristic profile of major withanolides and represented withaferin A, withanone, withanolide D or withanolide A rich groups. This level of phytochemical diversity as discrete chemotypes is widest and is being first ever documented to occur in Indian population of the plant. 相似文献
10.