Plasma concentrations of corticosterone and thyroid hormones in laying fowls from different housing systems     

S W Gibson  B O Hughes  S Harvey  P Dun 《British poultry science》1986,27(4):621-628

Plasma concentrations of corticosterone, thyroxine (T4) and triiodothyronine (T3) were measured in representative mature female domestic fowls from three housing systems: cages, covered strawyard and range. There were no between-system differences for T4 but T3 was lower in birds from range than in those from cages. Corticosterone concentrations were lower in birds from strawyards than in those from range and cages. In the strawyards there were no differences in corticosterone concentrations between normal and low-ranking hens. The latter were selected on the basis that they remained apart from the flock, were under-weight, poorly feathered and unable to feed at will. Low-ranking hens had significantly higher plasma concentrations of T3 and T4 than normal birds. This was attributed mainly to the poor feather covering of most low-ranking birds. It was concluded that, in the assessment of different housing systems, plasma corticosterone and thyroid hormones were not useful measures of long-term stress or welfare. Different factors relevant to welfare may have contrary effects on the plasma concentrations of these hormones.  相似文献   

Management of equine skull fractures using fixation with polydioxanone sutures     

KL Schaaf  NJ Kannegieter  DK Lovell 《Australian veterinary journal》2008,86(12):481-485

Ten horses presented with severe distortion of the facial contour, crepitus on palpation and mild to moderate epistaxis. Individual horses also showed ocular damage, ptosis, severe dyspnoea and movement of the facial bones concurrent with respiration. The fracture fragments were exposed using a large curvilinear incision and elevated using a retractor, periosteal elevator, chisel or Steinmann pin. The fracture fragments were unstable following reduction and fixation was necessary. Stabilisation was achieved with polydioxanone sutures placed through holes drilled in opposing sides of the fracture lines. Polydioxanone sutures provided good stability and had better handling properties than wire. There was good apposition of fracture edges and minimal complications. Use of polydioxanone sutures can also avoid the expense and complexity of plate fixation in selected cases, and should be considered as an alternative to fixation with stainless steel wire in any facial fracture that adjoins stable bone.  相似文献   

Isolation,Identification and Sequence Analysis of Immune and Virulence Genes of Four Pseudorabies Virus Strains from Hunan Province     

YANG Tao-tao  ZHAO Dun  LIU Chong-ling  QU Tai-long  YU Xing-long 《中国畜牧兽医》2016,43(1):50-57

To investigate molecular epidemiology of pseudorabies virus(PRV) in recent years in Hunan province and control pseudorabies more better, four wild-type PRV strains were detected from porcine brain tissues, which were collected from four farms of Pingjiang, Miluo, Liuyang and Changsha in Hunan province during 2012-2014.The four PRV strains were isolated by inoculating porcine brain tissues to the PK-15 cells, identified by PCR test and animal inoculation, and named PRV-XiangA, PRV-GA, PRV-YY and PRV-LY, respectively.The results showed the nucleotide homology among the four strains was 99.8% to 100.0%, by aligning the immune(gB, gG, gH, gI, gL and gM) and virulence genes(gE, PK and TK) of the four PRV strains, which indicated that these strains displayed almost no differences.The results of nucleotide alignment between the four strains and main strains isolated at home and abroad showed that the four strains had a high homology with PRV main strains isolated from China, with displaying nucleotide identity of 99.8% to 100.0% to BJ-YT and TJ strains, which revealed that the epidemic PRV strains in China had a little variation currently.  相似文献   

A survey of canine respiratory pathogens in New Zealand dogs     

HR Sowman  NJ Cave 《New Zealand veterinary journal》2018,66(5):236-242

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糠麸营养价值及其发酵饲料在动物生产中的应用     

崔艺燕  田志梅  鲁慧杰  邓盾  马现永  陈卫东 《中国畜牧兽医》2019,46(10):2902-2915

中国的谷物糠麸产量巨大,但未被充分利用,附加值低。小麦麸的粗蛋白质（CP）、粗纤维（CF）含量较高,吸水性较强,易霉变,易被呕吐毒素污染。米糠CP、粗脂肪（EE）含量较高,但易发生酸败。黑麦麸、燕麦麸、高粱糠的CP含量较高,而玉米皮CP、中性洗涤纤维（NDF）、酸性洗涤纤维（ADF）含量较高。糠麸矿物质丰富,还含有多种活性物质。而微生物发酵能够分解有害物质,增加食品、饲料中活性成分。通过微生物发酵能降低糠麸抗营养因子含量,将无法利用、利用率低的成分分解为易消化的小分子物质,提高蛋白质含量及消化率。目前糠麸发酵使用的微生物主要为霉菌、酵母、乳酸菌、芽孢杆菌,少部分使用食用真菌。研究表明,发酵糠麸富含有益代谢产物,能够为动物提供良好的抗氧化剂、益生菌来源,在合理利用糠麸的同时减少抗生素使用。发酵利用糠麸饲料能够达到一物多用,既降低生产成本,又解决废物排放造成的环境污染问题。作者综述了糠麸的营养价值、发酵菌种、发酵前后变化及其发酵饲料在动物生产的应用,以期为发酵糠麸的应用提供一定依据。  相似文献   

Prevalence of obesity and owners’ perceptions of body condition in pleasure horses and ponies in south‐eastern Australia          下载免费PDF全文

SJ Potter  NJ Bamford  PA Harris  SR Bailey 《Australian veterinary journal》2016,94(11):427-432

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农户信息需求调查与分析——以宁夏为例     

王盾 《农业图书情报学刊》2009,21(7):13-16

以宁夏519个农户为对象,采用问卷调查和半结构访谈方法,系统开展了不同经营类型农户信息意识、信息需求结构、获取渠道的对比分析和差异性研究,并探讨总结了造成不同类型农户信息需求差异性的原因。  相似文献   

流式细胞术(FCM)在生物学研究中的应用     

李靖  李成斌  顿文涛  王政  方庆 《勤云标准版测试》2008,(6)

流式细胞仪（Flow Cytometer）是一种对细胞进行定量分析与分选的精密仪器,它具有分析速度快、特异性好和灵敏度高的优点,是生物学研究的有力工具。流式细胞术（Flow Cytometry,FCM）是用流式细胞仪测量液相中悬浮细胞或微粒的一种现代分析技术,它是众多不同学术背景、不同科技领域相结合的结晶。概述了FCM在国内外生物学领域应用的最新动态。  相似文献   

甜高粱蔗糖合成酶基因（Susy2）的克隆及结构和功能分析   总被引：1，自引：0，他引：1  

刘洋  赵香娜  岳美琪  顿宝庆  张保明  路明  李桂英 《农业生物技术学报》2010,18(1):24-29

本研究以甘蔗蔗糖合成酶基因(susy2)cDNA序列为探针,对高粱EST数据库进行同源检索,将获得的4条与甘蔗相似性很高的EST序列进行拼接,后经基因组PCR、分子克隆和序列分析验证获得了甜高粱(sorghum bicofor)蔗糖合成酶基因DNA序列(Susy2,GenBank登录号为FJ513325).该序列全长4587 bp,起始密码子至终止密码子序列长4350 bp,包含一个2409 bp的开放读码框.该序列包含15个外显子和14个内含子,剪接方式都为GU/AG模式.Susy2编码的蛋白由802个氨基酸组成,分子量大小为91.7 kD,等电点pI为6.15.保守结构域分析表明,此蛋白含有一个475个氨基酸的GTI糖基化酶保守结构域(275～759),有4个ADP结合位点,能催化6-磷酸果糖和UDPG形成6-磷酸蔗糖.  相似文献   

被毛对热成像检测生猪体表温度精度的影响及噪声滤除方法     

贾桂锋  蒙俊宇  武墩  王登辉  高云  冯耀泽 《农业工程学报》2019,35(4):162-167

生猪皮肤的温度分布是表征其生理状态和疾病的重要指标,通常由红外热成像技术(infrared thermography, IRT)检测,然而由于生猪体表附有被毛在热图像中产生大量的温度噪声,降低了IRT对皮肤温度的检测精度。该文针对此问题探索被毛对皮肤温度分布的影响规律,并设计消除被毛影响的热图像降噪算法,提高对温度分布的检测精度。通过对12头生猪试验,分析目标区域在正常被毛和剔除被毛后温度分布的统计量得出被毛在温度分布中产生大量的"峡谷"状低温噪声,显著降低了目标区域的最低温度及平均温度。根据毛发噪声的影响规律提出网格化最大值-双三次插值算法并确定算法的最佳邻域尺寸为4.25mm。采用均方误差、峰值信噪比等指标定量评价算法的有效性,结果表明经算法处理后,均方误差由0.38下降到0.05(P0.01),峰值信噪比由45.14 dB上升到53.66 dB(P0.01),说明该算法能够滤除热图像中毛发引起的噪声,可提高IRT对温度分布的检测精度。  相似文献