Polymerase chain reaction for antigen receptor rearrangement: Benchmarking performance of a lymphoid clonality assay in diverse canine sample types     

E. J. Ehrhart  Shukmei Wong  Keith Richter  Victoria Zismann  Carolyn Grimes  William Hendricks  Chand Khanna 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2019,33(3):1392-1402

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Absorption of amino acids derived from laying hen rations containing protein components of plant origin. 1. Suboptimal lysine content     

K Gruhn  G Richter 《Archiv fuer experimentelle veterinaermedizin》1974,28(4):627-638

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Comparison between magnetic resonance imaging,computed tomography,and arthrography to identify artificially induced cartilage defects of the equine carpal joints          下载免费PDF全文

José Suarez Sanchez‐Andrade  Henning Richter  Karolin Kuhn  Andrea S. Bischofberger  Patrick R. Kircher  Séamus Hoey 《Veterinary radiology & ultrasound》2018,59(3):312-325

While articular cartilage changes are considered to be one of the initial events in the pathological cascade leading to osteoarthritis, these changes remain difficult to detect using conventional diagnostic imaging modalities such as plain radiography. The aim of this prospective, experimental, methods comparison study was to compare the sensitivity of magnetic resonance imaging (MRI), magnetic resonance arthrography, computed tomography (CT), and CT arthrography in the detection of artificially induced articular cartilage defects in the equine carpal joints. Defects were created in the antebrachiocarpal and middle carpal joint using curettage by a board‐certified equine surgeon. Normal articular cartilage thickness varied from a maximum of 1.22 mm at the level of the distal aspect of the radius to a minimum of 0.17 mm in the proximal articular surface of the third carpal bone. Regarding cartilaginous defect measurements the remaining cartilaginous bed range from a maximum of 0.776 mm in the partial thickness defects, and 0 mm (defect reaches the subchondral bone) when total thickness defect were made. Computed tomography and magnetic resonance imaging were performed followed by CT arthrography and magnetic resonance arthrography after antebrachiocarpal and middle carpal intraarticular contrast administration. All images were reviewed by two board‐certified veterinary radiologists, both of whom were blinded to the location, presence of, and thickness of the cartilage defects. A total number of 72 lesions in nine limbs were created. Mean sensitivity for localizing cartilage defects varied between imaging modalities with CT arthrography showing the best sensitivity (69.9%), followed by magnetic resonance arthrography (53.5%), MRI (33.3%), and CT (18.1%) respectively. The addition of contrast arthrography in both magnetic resonance and CT improved the rate of cartilage lesion detection although no statistical significance was found. Computed tomographic arthrography displayed the best sensitivity for detecting articular cartilage defects in the equine antebrachiocarpal and middle‐carpal joints, compared to magnetic resonance arthrography, MRI, and CT.  相似文献   

Plasma interleukin-6 response is predictive for severity and mortality in canine systemic inflammatory response syndrome and sepsis   总被引：4，自引：0，他引：4  

Rau S  Kohn B  Richter C  Fenske N  Küchenhoff H  Hartmann K  Härtle S  Kaspers B  Hirschberger J 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2007,36(3):253-260

BACKGROUND: Sepsis is still a major cause of death in both human and veterinary medicine. Early diagnosis is essential for appropriate treatment. Identification of patients at risk for developing sepsis is already possible in human medicine through the measurement of plasma interleukin-6 (IL-6) levels. In veterinary medicine, however, this has been investigated only in canine experimental models. OBJECTIVES: The purpose of this study was to measure IL-6 plasma levels in dogs with naturally occurring systemic inflammatory response syndrome (SIRS) and sepsis and to analyze the value of IL-6 as a predictive parameter for severity and mortality. METHODS: Included in the study were 79 dogs that had been admitted to the small animal clinics of Munich and Berlin from July 2004 to July 2005 and that satisfied the diagnostic criteria for SIRS and sepsis as defined using established parameters. Measurement of plasma IL-6 levels on days 0, 1, and 2 was performed by the use of a colorimetric bioassay based on IL-6-dependent cell growth. RESULTS: Septic foci were identified in 43 patients (septic group), and 36 patients were enrolled in the SIRS group. The frequency of positive blood cultures was 11%. The overall mortality rate was 48%. Higher plasma IL-6 levels on the day of admission were significantly correlated with a more severe degree of disease, increased mortality rate, and earlier fatality. CONCLUSIONS: Plasma IL-6 concentration is predictive of outcome in canine SIRS and sepsis and may be a valuable laboratory parameter for assessing critically ill dogs.  相似文献   

Measuring interlayer potassium release rates from soil materials. II. A percolation procedure to study the influence of the variable ‘solute K’ in the < 1…10 μM range     

Günther Springob  Jrg Richter 《植物养料与土壤学杂志》1998,161(3):323-329

Release rates of nonexchangeable K from Ap material of a Luvisol (‘Eckerde’ loess, 15% clay) are determined using a percolation procedure which avoids the common artifacts due to shaking or stirring soil suspensions and thus provides less biased kinetic data. CaCl₂ solution (10 mM_c. 20 °C. pH 5.8) is percolated through packages of soil aggregates (0.5–1 mm grain size, 0.5 g samples) with 0.02 to 25 ml h^?1. Solute K (C_K) was varied between < 1 and > 10 μM and is shown to have a dominant influence on the rates of interlayer K release. These increase exponentially below 3.5 μM (no steady state but steady decrease of release rates in each sample). The difference between the related C_K between moderate and high release rates is as small as 1 μM K. The average rates, of a 10-day-interval, starting after 1.3 times the exchangeable K had been removed, are 40 μmol K kg^?1 soil d^?1 at 4 μM C_K and 240 μmol kg^?1 d^?1 at 3 μM C_K, respectively. It is concluded that larger quantities of interlayer K become plant available in the studied soil if the of soil solutions gets below 3.5 μM (for 10 mM_c Ca, 20 °C, pH 5.8), probably because the dominant dioctahedral illites start to join the release process below this critical limit. The higher K concentration range was accounted for by K-Ca exchange isotherms which, by alteration of shape, indicate that K_nex release becomes measurable below 10 to 20 μM K. It is further argued that existing diffusion or reaction kinetics approaches towards K release are incomplete because the influence of solute K is not considered.  相似文献   

A dielectric omnidirectional reflector   总被引：3，自引：0，他引：3  

Y Fink  JN Winn  S Fan  C Chen  J Michel  JD Joannopoulos  EL Thomas 《Science (New York, N.Y.)》1998,282(5394):1679-1682

A design criterion that permits truly omnidirectional reflectivity for all polarizations of incident light over a wide selectable range of frequencies was used in fabricating an all-dielectric omnidirectional reflector consisting of multilayer films. The reflector was simply constructed as a stack of nine alternating micrometer-thick layers of polystyrene and tellurium and demonstrates omnidirectional reflection over the wavelength range from 10 to 15 micrometers. Because the omnidirectionality criterion is general, it can be used to design omnidirectional reflectors in many frequency ranges of interest. Potential uses depend on the geometry of the system. For example, coating of an enclosure will result in an optical cavity. A hollow tube will produce a low-loss, broadband waveguide, whereas a planar film could be used as an efficient radiative heat barrier or collector in thermoelectric devices.  相似文献   

Tracking the long-term decline and recovery of an isolated population     

RL Westemeier  JD Brawn  SA Simpson  TL Esker  RW Jansen  JW Walk  EL Kershner  JL Bouzat  KN Paige 《Science (New York, N.Y.)》1998,282(5394):1695-1698

Effects of small population size and reduced genetic variation on the viability of wild animal populations remain controversial. During a 35-year study of a remnant population of greater prairie chickens, population size decreased from 2000 individuals in 1962 to fewer than 50 by 1994. Concurrently, both fitness, as measured by fertility and hatching rates of eggs, and genetic diversity declined significantly. Conservation measures initiated in 1992 with translocations of birds from large, genetically diverse populations restored egg viability. Thus, sufficient genetic resources appear to be critical for maintaining populations of greater prairie chickens.  相似文献   

Assignment of new loci to river buffalo chromosomes confirms the nature of chromosomes 4 and 5     

By S. M. EL NAHAS  H. A. HONDT  S. F. SOUSSA  A. EL GHOR  A. A. HASSAN 《Zeitschrift für Tierzüchtung und Züchtungsbiologie》1999,116(1):21-28

Introduction Rapid development of the river buffalo physical map can be achieved by coupling its development to that of the cattle gene map. Syntenic conservation between cattle and buffalo has been demonstrated, mainly using somatic cell hybrids (de Hondt et al. 1991; El Nahas et al. 1993, 1996, 1998; de Hondt et al. 1997; El Nahta 1996; Oraby et al. 1977), and by using in situ hybridization as reviewed by Iannuzzi (1997). G- and R-banding comparisons between cattle (2n = 60) and river buffalo (2n = 50) chromosomes have revealed a large number of banding homologies between the two species, both at early-metaphase (Gupta and Ray -Chaudhury 1978; Di Berardino et al. 1981) and prometaphase stages (Iannuzzi et al. 1990). Banding homology indicates that the five river buffalo biarmed pairs originate from centric fusion translocation between two of ten homologous cattle autosomes, which is very supportive of the hypothesis that both species have a common ancestor (Wurster and Benirschke 1968). Based on cytological analysis and banding homology between cattle and buffalo chromosomes, the five biarmed chromosomes of the river buffalo BBU1, BBU2, BBU3, BBU4, BBU5 were thought to originate from fusion of cattle chromosome (BTA) 1/25; 2/23; 8/19; 5/28; and 16/29 respectively (Iannuzzi et al. 1990; Report of the Committee for the Standardization of Banded Karyotopes of the River Buffalo 1994). However, the analysis of synteny between molecular markers assigned to different cattle syntenic groups demonstrated that BBU1 results from fusion of BTA 1 and 27 rather than 1 and 25 (El Nahas et al. 1977). This called for expanding the analysis of syntenic relationships between marker loci to confirm the nature of the other biarmed buffalo chromosomes. The purpose of this study is to test synteny between markers in buffalo and to confirm the nature of the biarmed buffalo chromosomes 4 and 5, using marker loci and somatic cell hybrids.  相似文献   

Rice with fish culture in the semi-deep waters of the Mekong Delta, Vietnam: a socio-economical survey   总被引：1，自引：0，他引：1  

A J Rothuis  D K Nhan  C JJ Richter  F Ollevier 《Aquaculture Research》1998,29(1):47-57

The results of a socio-economical survey of farming systems practising rice culture with introduced fish, rice culture with indigenous fish and rice monoculture in the semi-deep waters of the Mekong Delta, Vietnam, are presented. Rice and fish yields, inputs, and cost-benefits were computed to evaluate the agricultural effects of rice with fish culture. Household size, labour availability and educational level were not significantly different among the three farming systems. Rice-fish farming systems differed mainly from rice monoculture by a higher fertilizer/ water requirement and less pesticide use. The total farm cash and net return did not differ among the farming systems. The main beneficial effects of rice-fish culture are thought to be related to environmental sustainability, system biodiversity, farm diversification and household nutrition.  相似文献   

C and N accumulation in arable soils of West Germany and its influence on the environment — Developments 1970 to 1998     

Rolf Nieder  Jrg Richter 《植物养料与土壤学杂志》2000,163(1):65-72

During the last three decades, large amounts of soil organic matter (SOM) and associated nutrients have been accumulated in arable soils of Western Germany (former FRG) due to deepening of the plough layers (from < 25 to > 35 cm) and to fertilizer application rates which have exceeded the amounts of nutrients removed in harvested crops. Organic carbon and total nitrogen balances (1970—1998) on 120 plots from 16 farms in southern Lower Saxony yielded a cumulative increase of up to 16 t C ha⁻¹ and 1 t N ha⁻¹ in loess soils used for cash crop production and up to 26 t C ha⁻¹ and 2.4 t N ha⁻¹ in sandy soils under livestock production. The buffering capacity for reactive compounds, particularly of C, N, S and P and of other (organic or inorganic) pollutants will reach its limits in the near future, after organic matter ”︁equilibria” have been re‐established. An immediate adaptation of the current fertilizer application rates to the nutrient export by field crops is therefore urgently needed.  相似文献