A comparison of the cytologic and histologic features of meningiomas in four dogs     

Zimmerman KL  Bender HS  Boon GD  Prater MR  Thorn CE  Prater D  Robertson JL  Saunders GK  Sponenberg DP  Inzana KD  Lanz OI  Wright E 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2000,29(1):29-34

Abstract: The cytologic and histologic features of 2 intracranial and 2 spinal (extramedullary cervical) canine meningiomas were compared. Cerebrospinal fluid analysis in 2 cases revealed mild, mixed cell pleocytosis, primarily composed of small lymphocytes and monocytoid cells, with a moderate increase in total protein concentration. Cytologic features suggestive of meningioma included cells with both epithelial and mes-enchymal characteristics and a tendency towards cell clustering. Tumor location also was useful in making a diagnosis. The 4 meningiomas differed histologically from one another, and included angioblastic, psam-momatous, meningotheliomatous, and microcystic anaplastic types, which conformed to a classification scheme for human meningiomas. The classification scheme could not be applied to cytologic specimens.  相似文献   

Bush cover and range condition assessments in relation to landscape and grazing in southern Ethiopia   总被引：6，自引：0，他引：6  

Oba  Gufu  Post  Eric  Syvertsen  P.O.  Stenseth  N.C. 《Landscape Ecology》2000,15(6):535-546

Progressive growth of bush cover in dry savannahs is responsible for declines in range conditions. In southern Ethiopia, the Booran pastoralists assisted our understanding of spatial patterns of bush cover and range conditions in 54 landscape patch types grouped into six landscape units within an area of 30000 km². The size of landscape patches sampled was 625 m². We assessed the relationships between bush cover, grass cover and bare soil and grazing pressure and soil erosion and changes in range condition. Externally, political conflicts and internally, break down of land use, and official bans on the use of fire promoted bush cover and the decline in range conditions. Bush cover was negatively correlated with grass cover, and positively correlated with bare soil. Grass cover was negatively correlated with bare soil and grazing pressure in most landscape patch types. Grazing pressure was not significantly correlated with bush cover or bare soil, while soil erosion was directly related to bare soil. Soil erosion was absent in 64% of the landscape patch types, and seemingly not a threat to the rangelands. The relationship between bush cover, grass cover, bare soil and soil erosion is complex and related to climate, landscape geology, and patterns of land use. Main threats to range conditions are bush climax, loss of grass cover and unpalatable forbs. Currently, >70% of the landscape patch types are in poor to fair range conditions. Decline in range conditions, unless reversed, will jeopardise the pastoral production system in southern Ethiopia.  相似文献   

Evaluation of cardiac performance of the dog after induction of portal hypertension and gastric ischemia     

Catriona M. MacPhail  DVM  DACVS    Eric Monnet  DVM  PhD  DACVS  DECVS    Davyd H. Pelsue  DVM  DACVS    James S. Gaynor  DVM  MS  DACVA 《Journal of Veterinary Emergency and Critical Care》2006,16(3):192-198

Objective: To determine changes in hemodynamic and cardiac energetic parameters in dogs after induction of portal hypertension and gastric ischemia. These blood flow alterations are similar to changes seen in splanchnic blood flow in dogs with gastric dilatation volvulus syndrome (GDV). Design: Original experimental study. Setting: Veterinary teaching hospital. Animals: Seven purpose‐bred, intact male dogs. Interventions: Standard midline laparotomy and median sternotomy were performed under general anesthesia. Dogs were instrumented to obtain arterial blood pressure, aortic flow, cardiac chamber pressures, central venous pressure, portal flow, and portal pressure. Colored microsphere technology was used for the determination of myocardial blood flow. Measurements and samples were obtained at baseline, following induction of portal hypertension, and after induction of portal hypertension and gastric ischemia. Measurements and main results: Left ventricular myocardial blood flow was increased from 81.8±20.1 mL/100 g/min at baseline to 127.7±57.2 mL/100 g/min (P=0.02) after induction of portal hypertension and gastric ischemia. Myocardial oxygen consumption increased from 142.2±27.4 J/min/100 g at baseline to 219.1±33.4 J/min/100 g (P=0.003) after induction of portal hypertension and gastric ischemia, but cardiac external work remained unchanged (13.67±6.2 to 13.27±9.6 J/min; P=0.78; power=0.79). Cardiac efficiency decreased from 11.6±6.1% at baseline to 7.6±5.1% (P=0.017) after induction of portal hypertension and gastric ischemia. Conclusions: Transfer of energy within the myocardium was less efficient after induction of portal hypertension and ischemia of the stomach wall. On the basis of these results, alterations in cardiac function associated with GDV may result from deterioration of cardiac efficiency.  相似文献   

Simulating the cumulative effects of multiple forest management strategies on landscape measures of forest sustainability   总被引：1，自引：2，他引：1  

Eric J. Gustafson  David E. Lytle  Randy Swaty  Craig Loehle 《Landscape Ecology》2007,22(1):141-156

While the cumulative effects of the actions of multiple owners have long been recognized as critically relevant to efforts to maintain sustainable forests at the landscape scale, few studies have addressed these effects. We used the HARVEST timber harvest simulator to predict the cumulative effects of four owner groups (two paper companies, a state forest and non-industrial private owners) with different management objectives on landscape pattern in an upper Michigan landscape managed primarily for timber production. We quantified trends in landscape pattern metrics that were linked to Montreal Process indicators of forest sustainability, and used a simple wildlife habitat model to project habitat trends. Our results showed that most trends were considered favorable for forest sustainability, but that some were not. The proportion of all age classes and some forest types moved closer to presettlement conditions. The trend for the size of uneven-aged patches was essentially flat while the average size of patches of the oldest and youngest age classes increased and the size of patches of the remaining age classes decreased. Forest fragmentation generally declined, but edge density of age classes increased. Late seral forest habitat increased while early successional habitat declined. The owners use different management systems that cumulatively produce a diversity of habitats. Our approach provides a tool to evaluate such cumulative effects on other landscapes owned by multiple owners. The approach holds promise for helping landowner groups develop and evaluate cooperative strategies to improve landscape patterns for forest sustainability.  相似文献   

Protocol: a highly sensitive RT-PCR method for detection and quantification of microRNAs   总被引：5，自引：0，他引：5  

Erika Varkonyi-Gasic  Rongmei Wu  Marion Wood  Eric F Walton  Roger P Hellens 《Plant methods》2007,3(1):12

MicroRNAs (miRNAs) are a class of small non-coding RNAs with a critical role in development and environmental responses. Efficient and reliable detection of miRNAs is an essential step towards understanding their roles in specific cells and tissues. However, gel-based assays currently used to detect miRNAs are very limited in terms of throughput, sensitivity and specificity. Here we provide protocols for detection and quantification of miRNAs by RT-PCR. We describe an end-point and real-time looped RT-PCR procedure and demonstrate detection of miRNAs from as little as 20 pg of plant tissue total RNA and from total RNA isolated from as little as 0.1 μl of phloem sap. In addition, we have developed an alternative real-time PCR assay that can further improve specificity when detecting low abundant miRNAs. Using this assay, we have demonstrated that miRNAs are differentially expressed in the phloem sap and the surrounding vascular tissue. This method enables fast, sensitive and specific miRNA expression profiling and is suitable for facilitation of high-throughput detection and quantification of miRNA expression.  相似文献   

Host status and reaction of <Emphasis Type="Italic">Medicago truncatula</Emphasis> accessions to infection by three major pathogens of pea (<Emphasis Type="Italic">Pisum sativum</Emphasis>) and alfalfa (<Emphasis Type="Italic">Medicago sativa</Emphasis>)     

Anne Moussart  Caroline Onfroy  Angelique Lesne  Magali Esquibet  Eric Grenier  Bernard Tivoli 《European journal of plant pathology / European Foundation for Plant Pathology》2007,117(1):57-69

Ditylenchus dipsaci, the stem nematode of alfalfa (Medicago sativa), Mycosphaerella pinodes, cause of Ascochyta blight in pea (Pisum sativum) and Aphanomyces euteiches, cause of pea root rot, result in major yield losses in French alfalfa and pea crops. These diseases are difficult to control and the partial resistances currently available are not effective enough. Medicago truncatula, the barrel medic, is the legume model for genetic studies, which should lead to the identification and characterization of new resistance genes for pathogens. We evaluated a collection of 34 accessions of M. truncatula and nine accessions from three other species (two from M. italica, six from M. littoralis and one from M. polymorpha) for resistance to these three major diseases. We developed screening tests, including standard host references, for each pathogen. Most of the accessions tested were resistant to D. dipsaci, with only three accessions classified as susceptible. A very high level of resistance to M. pinodes was observed among the accessions, none of which was susceptible to this pathogen. Conversely, a high level of variation, from resistant to susceptible accessions, was identified in response to infection by A. euteiches.  相似文献   

D. McKenzie, C. Miller and D.A. Falk (eds.): The Landscape Ecology of Fire     

Eric J. Gustafson 《Landscape Ecology》2012,27(6):925-926

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Acute Reactions in Dogs Treated with Doxorubicin: Increased Frequency with the Use of a Generic Formulation     

Brenda S. Phillips  Susan A. Kraegel  Eric Simonson  Bruce R. Madewell 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》1998,12(3):171-172

During a 4-month period, 34 dogs with tumors received a total of 60 doses of a single generic formulation of doxorubicin; 13 acute drug reactions were observed in these 34 dogs, and no acute reactions were observed after replacing the product with the proprietary brand. These reactions were characterized by one or more of the following signs: pruritus; head-shaking; urticaria; erythema of the pinnal, axillary, or inguinal regions; vocalization; vomiting; hyperemic or pale mucous membranes; high heart rate; and high respiratory rate. We propose that a component unique to generic doxorubicin was responsible for the unusually high number of acute drug reactions observed.  相似文献   

Impact of successive drought and re-watering cycles on growth and specific leaf area of two Populus x canadensis (Moench) clones, 'Dorskamp' and 'Luisa_Avanzo'     

Marron N  Dreyer E  Boudouresque E  Delay D  Petit JM  Delmotte FM  Brignolas F 《Tree physiology》2003,23(18):1225-1235

Responses to successive drought and re-watering cycles (1-3 cycles) were compared in greenhouse-grown cuttings of Populus x canadensis (Moench) clones, 'Luisa_ Avanzo' and 'Dorskamp.' Total leaf number increment rate, duration of leaf expansion, total and individual leaf area expansion rates and stomatal conductance were recorded periodically during the experiment. Soil water content (SWC) and predawn leaf water potential (Psi(WP)) were measured four times during each drought cycle. In parallel, relative leaf water content (RWC) and specific leaf area (SLA) were estimated on leaves collected from the top to bottom of each cutting. Under well-watered conditions, 'Luisa_Avanzo' and 'Dorskamp' differed in their patterns of leaf area expansion. Although duration of leaf expansion was similar between clones, 'Luisa_ Avanzo' exhibited higher total leaf number increment rates and individual leaf area increases than 'Dorskamp.' As a result, 'Luisa_Avanzo' cuttings reached larger individual and total leaf areas than 'Dorskamp.' 'Dorskamp' leaves had lower SLA than 'Luisa_Avanzo' leaves. In response to successive drought cycles, both clones underwent decreases in total leaf number increment rates and in total leaf area expansion rates, but both whole-plant and individual leaf areas were drastically reduced only in 'Luisa_Avanzo.' 'Dorskamp' maintained a constant leaf area as a result of an increase in the duration of leaf expansion during drought and a significant stimulation of individual leaf area expansion rate and total leaf number increment rate in response to re-watering. Drought caused a greater decrease in SLA in 'Luisa_Avanzo' than in 'Dorskamp.' Expanded leaves of 'Dorskamp' were constitutively dense or thick, or both, whereas leaves of 'Luisa_Avanzo' became dense or thick, or both, only in response to drought. In both clones, re-watering caused partial recovery of SLA to control values. Our data confirm previous field and greenhouse observations that 'Dorskamp' is more tolerant to successive drought and re-watering cycles than 'Luisa_Avanzo.' We conclude that this difference is mainly attributable to the plasticity of 'Dorskamp' after re-watering, rather than to clonal differences in drought responses.  相似文献   

Kinetics of leaf temperature fluctuation affect isoprene emission from red oak (Quercus rubra) leaves     

Singsaas EL  Laporte MM  Shi JZ  Monson RK  Bowling DR  Johnson K  Lerdau M  Jasentuliytana A  Sharkey TD 《Tree physiology》1999,19(14):917-924

Because the rate of isoprene (2-methyl-1,3-butadiene) emission from plants is highly temperature-dependent, we investigated natural fluctuations in leaf temperature and effects of rapid temperature change on isoprene emission of red oak (Quercus rubra L.) leaves at the top of the canopy at Harvard Forest. Throughout the day, leaves often reached temperatures as much as 15 degrees C above air temperature. The highest temperatures were reached for only a few seconds at a time. We compared isoprene emission rates measured when leaf temperature was changed rapidly with those measured when temperature was changed slowly. In all cases, isoprene emission rate increased with increasing leaf temperature up to about 32 degrees C and then decreased with higher temperatures. The temperature at which isoprene emission rates began to decrease depended on how quickly measurements were made. Isoprene emission rates peaked at 32.5 degrees C when measured hourly, whereas rates peaked at 39 degrees C when measurements were made every four minutes. This behavior reflected the rapid increase in isoprene emission rate that occurred immediately after an increase in leaf temperature, and the subsequent decrease in isoprene emission rate when leaf temperature was held steady for longer than 20 minutes. We concluded that the observed temperature response of isoprene emission rate is a function of measurement protocol. Omitting this parameter from isoprene emission models will not affect simulated isoprene emission rates at mild temperatures, but can increase isoprene emission rates at high temperatures.  相似文献