全文获取类型
| 收费全文 | 164篇 |
| 免费 | 10篇 |
专业分类
| 林业 | 10篇 |
| 农学 | 8篇 |
| 40篇 | |
| 综合类 | 16篇 |
| 农作物 | 4篇 |
| 水产渔业 | 9篇 |
| 畜牧兽医 | 73篇 |
| 园艺 | 1篇 |
| 植物保护 | 13篇 |
出版年
| 2023年 | 5篇 |
| 2022年 | 2篇 |
| 2021年 | 4篇 |
| 2020年 | 6篇 |
| 2019年 | 7篇 |
| 2018年 | 2篇 |
| 2017年 | 7篇 |
| 2016年 | 3篇 |
| 2015年 | 7篇 |
| 2014年 | 9篇 |
| 2013年 | 17篇 |
| 2012年 | 20篇 |
| 2011年 | 19篇 |
| 2010年 | 5篇 |
| 2009年 | 8篇 |
| 2008年 | 9篇 |
| 2007年 | 8篇 |
| 2006年 | 7篇 |
| 2005年 | 10篇 |
| 2004年 | 5篇 |
| 2003年 | 3篇 |
| 2002年 | 7篇 |
| 2001年 | 2篇 |
| 1998年 | 1篇 |
| 1990年 | 1篇 |
排序方式： 共有174条查询结果，搜索用时 203 毫秒
21.
Victor JV Rossetto Lígia SLS da Mota Noeme S Rocha Hélio A Miot Fabrizio Grandi Cláudia VS Brand?o 《Acta veterinaria Scandinavica》2013,55(1):39
Background
Due to numerous complications associated to gastrointestinal augmented cystoplasty, this study aimed to analyze the anatomic repair of the bladder of 10 female dogs using grafts of porcine small intestinal submucosa (SIS) seeded with cultured homologous smooth muscle cells, and compare them with the acellular SIS grafts.Results
We assessed the possible side effects and complications of each type of graft by clinical examination, abdominal ultrasound and laboratory findings. Anatomic repair of neoformed bladder was assessed by histological staining for H/E and Masson''s Trichrome, analyzed with a Nikon Photomicroscope connected to the system of image analysis Image J.Conclusions
We propose that SIS associated to homologous smooth cells can improve the quality of tissue repair, and consequently decrease the potential complications inherent to acellular SIS. 相似文献22.
Organic wastes can be usefully recovered to produce organic amendments, for example, compost, to be used for crop production, thus reducing impacts through efficient waste management. The aim of this work was to study the effects of compost obtained from municipal waste in combination with poultry manure on plant growth, nitrate reductase (NR) activity and absorption and distribution of heavy metals (HM) in plant tissues of tomatoes, grown in pots in greenhouses. Two compost types obtained from municipal waste mixed with poultry manure (C1?=?3:2 and C2?=?2:3) were used at two different ages (105 d and 173 d) and at two mix rates with soil (32.5?g pot?1 and 65?g pot?1); soil with no compost amendment was used as control. The experiment was conducted using tomato plants in pots and plant growth and nutrient plant uptake was determined after 65?days from plant transplanting. Results obtained indicated that compost type and compost rate affected biomass production. However, compost age did not influence the development of plants. Nutrient status of tomato plants was also investigated with reference to the N cycle. Nitrite accumulation in the leaves increased with the increase in compost doses. The accumulation of NO2? was associated with a significant increase in NR activity. HM content in leaves decreased with compost use. HM accumulated preferentially in roots and leaves and the soil to root metals transfer was in this order: Fe (1.08–2.14)> Co (0.53–4.10)>Cu (0.28–2.28) >Mn (0.3–1.34) >Zn (0.87–1.21)>Cr (0.12–1.64). The highest and lowest dynamic bioaccumulation factors (BAFdyn) were observed in roots and stems, respectively. The root system acted as a barrier for Cd and Pb. It was concluded that compost use is beneficial for tomato plants, with particular reference to the compost obtained by using a higher amount of poultry manure (C2) in the mix. 相似文献
23.
Paltrinieri S Comazzi S Ceciliani F Prohaska R Bonfanti U 《Veterinary journal (London, England : 1997)》2007,173(1):200-203
Stomatocytosis resembles human overhydrated hereditary stomatocytosis (OHSt), a disease characterised by a reduced or absent stomatin expression. The objective of this report was to investigate the expression level of stomatin in erythrocytes from Standard Schnauzers with stomatocytosis. Routine haematology, intraerythrocytic Na(+)/K(+) concentration and stomatin expression were evaluated in blood from twelve Standard Schnauzers and from three controls. SDS-PAGE and Western blotting on isolated integral membrane proteins were used to investigate stomatin expression. Circulating stomatocytes, macrocytosis, anisocytosis, increased erythrocyte fragility and high intracellular sodium and potassium concentrations were found in 10/12 dogs from the same breeding line although stomatin levels were similar to those of controls. In spite of the clinico-pathological similarities between human and canine stomatocytosis, erythrocytes from affected dogs do not lack stomatin and the expression level of this protein cannot therefore be used to diagnose hereditary stomatocytosis in Standard Schnauzers. 相似文献
24.
25.
26.
Simon J. More Angus R. Cameron Matthias Greiner Richard S. Clifton-Hadley Sandra Correia Rodeia Douwe Bakker Mo D. Salman J. Michael Sharp Fabrizio De Massis Alicia Aranaz M. Beatrice Boniotti Alessandra Gaffuri Per Have Didier Verloo Michael Woodford Martin Wierup 《Preventive veterinary medicine》2009,90(3-4):254-267
Within the European Union (EU), detailed legislation has been developed for cattle, but not deer, to minimise disease risks associated with trade in animals and animal products. This legislation is expressed as input-based standards, providing a detailed outline of the activity required (for example, testing of animals and application of defined control measures), on the expectation that an adequate output (for example, confidence in freedom) will be achieved. Input-based standards are at odds with the increasing shift towards output-based standards, particularly in OIE rules governing international trade. In this paper, we define output-based standards to achieve and maintain freedom from tuberculosis (TB) in farmed deer, with reference to EU member states. After considering the probability of freedom achieved for cattle under existing EU legislation, we defined a ‘free farmed deer holding’ as one with a probability of freedom from infection of at least 99%. We then developed an epidemiological model of TB surveillance systems for deer holdings, incorporating different surveillance strategies, including combinations of diagnostic tests, and a variety of different scenarios relating to the potential for introduction of infection. A range of surveillance strategies were identified to achieve and maintain a free farmed deer holding, and worked examples are presented. The surveillance system sensitivity for varying combinations of screening and confirmatory tests in live animals, animals at slaughter and on-farm deaths is also presented. Using a single test at a single point in time, none of the TB tests routinely used in farmed deer is able to achieve an acceptable probability of TB freedom. If repeat testing were undertaken, an acceptable probability of TB freedom could be achieved, with differing combinations of the surveillance system sensitivity, frequency of testing and risk of introduction. The probability of introduction of infection through the importation of infected deer was influenced by the use of a pre-movement test (assumed 90% test sensitivity and negative test results), the TB prevalence in the source herd and the number of animals imported. A surveillance system sensitivity of at least 81% was achieved with different combinations of annual live animal surveillance and surveillance of animals at slaughter or on-farm deaths. This methodology has broad applicability and could also be extended to other diseases in both deer and other species with relevance to trade in animals and animal products. 相似文献
27.
Lecchi C Avallone G Giurovich M Roccabianca P Ceciliani F 《Veterinary journal (London, England : 1997)》2009,180(2):256-258
Combined quantitative (real-time) polymerase chain reaction and immunohistochemistry were used to evaluate the expression of the minor acute phase protein alpha 1-acid glycoprotein (AGP, orosomucoid) in bovine extrahepatic tissues. AGP was produced mainly in the salivary glands and spleen, whereas minor expression was detected in all other tissues sampled, including lung, lymph nodes, uterus, ovary, kidney and tongue. The findings were consistent with immunohistochemical results. In view of the immunomodulatory and direct antibacterial activity of AGP, its expression in the salivary glands may signal an involvement in the regulation of the local immunity, even in non-pathological conditions. 相似文献
28.
29.
30.