Genetics of soybean-Heterodera glycines system     

S. C. Anand  S. B. Sharma 《Integrated Pest Management Reviews》1995,1(1):65-69

Genetics of resistance to soybean cyst nematode (SCN), Heterodera glycines Ichinohe is very complex. Crosses involving PI 437654, which is resistant to all races of cyst nematodes with other sources of resistance (Peking, PI 88788, and PI 90763) indicated that resistance to race 3 was controlled by four genes, two of which were dominant resistance genes and the other two were recessive resistance genes. For race 5, a four gene model with two recessive and two dominant resistance genes in epistasis has been proposed. For race 14, the results suggested a three gene model with one dominant and two recessive alleles. Several other plant introductions have been isolated which have different genes conditioning resistance. Most of the currently grown soybean varieties derived resistance from Peking and/or PI 88788. Resistance to SCN in these soybean varieties has broken down because of the emergence of several new races and populations of SCN. The use of PI 437654 or Hartwig and other plant introductions with different genes for resistance will broaden genetic diversity and stabilize yield.  相似文献   

The effects of bovine respiratory syncytial on normal ovine lymphocyte responses to mitogens or antigens in vitro     

Keles I  Sharma AK  Woldehiwet Z  Murray RD 《Comparative immunology, microbiology and infectious diseases》1999,22(1):1-13

In the present study peripheral blod mononuclear cells (MNC) obtained from normal uninfected lambs were used to study the possible effects of bovine respiratory syncytial virus (BRSV) on lymphocyte responses to the mitogens, phytohaemagglutinin (PHA), concanavalin A (Con A) and pokeweed mitogen (PWM) in vitro. Live BRSV had a depressive effect on the proliferative responses of normal MNC to PHA, Con A and PWM. Inactivated BRSV and a commercial preparation of prostaglandin E2 were also found to depress the proliferative responses of normal ovine MNC to PHA but recombinant tumour necrosis factor-alpha (TNF-alpha) had no such effect. Serum samples obtained from BRSV-infected lambs contained substances inhibitory to PHA-driven lymphocyte blastogenesis. Memory blastogenic responses to border disease virus (BDV) of lymyphocytes obtained from lambs previously primed with BDV were significantly reduced when lymphocytes were exposed to infectious BRSV.  相似文献   

Comparative pathogenesis of serotype 1 and variant serotype 1 isolates of infectious bursal disease virus and their effect on humoral and cellular immune competence of specific-pathogen-free chickens   总被引：3，自引：0，他引：3  

J M Sharma  J E Dohms  A L Metz 《Avian diseases》1989,33(1):112-124

Specific-pathogen-free chickens inoculated with isolate VA (variant A) or isolate IM of infectious bursal disease virus (IBDV) were examined for mitogenic response to T-cell mitogens, primary and secondary antibody response to sheep erythrocytes and Brucella abortus, and gross and histologic lesions in thymus and bursa. Both isolates induced comparable depression in the mitogenic and antibody response, and both caused extensive gross and histologic lesions in the bursa of Fabricius. However, bursal necrosis induced by the IM isolate was accompanied by an inflammatory response, whereas the inflammatory component was lacking in the lesion induced by the VA isolate. Furthermore, the IM isolate induced extensive lesions in the thymus, but the VA isolate did not.  相似文献   

Evaluation of Cytotoxic Responses Caused by Selected Organophosphorus Esters in Chick Sympathetic Ganglia Cultures          下载免费PDF全文

E. J. Obersteiner  R. P. Sharma 《Canadian journal of veterinary research》1978,42(1):80-88

Ten day old chick sympathetic ganglia cultured in a microslide assembly were treated with a selected group of organophosphate pesticides to evaluate their cytotoxicity ranges, and the usefulness of such a model for screening pesticides. Examination by phase contrast and light microscopy for chemically-induced morphological alteration of nerve fibers, glial cells and neurons provided the criteria for quantitation and assessment of the toxic effects. Concentrations that produced half-maximal effects ranged from 1 × 10^-6M (severely toxic) for methylparathian, diazinon, paraoxon, mevinphos, diisopropylfluorophosphate, tri-o-tolyl phosphate and its mixed isomers to a 1 × 10^-3M (intermediate) for malathion, leptophos, coumaphos, mono- and dicrotophos. Some or no effects were evident at 1 × 10^2-M for O'ethyl-O-p-nitrophenyl phenyl phosphonothioate, tri-m-tolylphosphate, chlorpyriphos and triphenyl phosphate. In all instances, nerve fibers were more sensitive than neurons or glial cells to insecticides. All cellular growth was inhibited at 1 × 10^-2M (except triphenyl phosphate). Below 1 x 10^-7M, no inhibitory effects were evident. The secondary abnormalities included decreased cellular migration, diffuse cellular growth pattern, increased vacuolization, nerve fiber swelling and cellular degeneration. The cytotoxic effects of these chemicals do not appear to be related to in vivo toxicity or cholinesterase inhibition potential.  相似文献   

A fatal cutaneous granuloma due to Entomophthora coronata in a mare   总被引：2，自引：0，他引：2  

H V Chauhan  G L Sharma  D S Kalra  F C Malhotra  M P Kapur 《The Veterinary record》1973,92(16):425-427

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Observations on the incidence and histopathology of pneumonia of sheep and goats in India     

S Ramachandran  G L Sharma 《The Indian veterinary journal》1969,46(1):16-29

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Studies on oestrous cycle in Hariana cows     

O P Sharma  B P Singh  N S Tomar 《The Indian veterinary journal》1968,45(12):1014-1022

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Detection of rinderpest antibodies     

R. C. Joshi  B. Sharma  S. K. Bandyopadhyay  R. P. Bansal 《Tropical animal health and production》1984,16(3):167-170

Rinderpest antibodies were detected by employing the fluorescent antibody test (FAT) and the immunoperoxidase test (IPT) and the results were compared with the counterimmuno electrophoresis test (CIE). FAT was found to be the most sensitive in detecting post-vaccinal antibodies followed by IPT and CIE tests.  相似文献   

CONTRAST RADIOGRAPHY OF THE OVINE GASTROINTESTINAL TRACT     

Sandeep K.  Sharma  MVSc  Ambika P.  Singh  MVSc  PhD  Rishi  Tayal  MVSc  Inder S.  Chandna  MVSc  PhD 《Veterinary radiology & ultrasound》1984,25(1):17-22

Contrast radiography of the alimentary canal was conducted in six adult sheep with barium sulfate (70% W/V, 25–30 ml/kg). A period of 12 hours was adequate for opacification of most parts of the gastrointestinal tract. The presence of contrast medium in different parts of the alimentary canal was noted for as long as 60 hours. The right lateral view was adequate for visualization of most structures, but the typical laminar mucosal pattern of the omasum and the spiral mucosal folds of the abomasum were demonstrated better on the ventrodorsal view.  相似文献   

Haemorrhagic syndrome in poultry     

U K Sharma  N Singh 《The Indian veterinary journal》1971,48(3):235-238

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