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排序方式: 共有183条查询结果,搜索用时 250 毫秒
61.
草原红牛转铁蛋白和后转铁蛋白多态性及其与生产性能相关性研究(英文) 总被引:4,自引:0,他引:4
[Objective] The aim of this study was to investigate the correlation between blood protein polymorphism of red steppe and its performance.[Method]Two blood protein polymorphic loci were detected in transferring(Tf)and posttremsferr(Ptf)from thirteen red steppes and eighteen hybrid of limousin and red steppe by polyacrylamide gel electrophoresis.[Result]Tf and Ptf were controlled by three and two alleles respectively.[Conclusion]The variance analysis of blood protein polymorphic loci and its performance indicates that two protein loci have a positive or negative correlation with some traits of red steppe and the improved limousin cattle population. 相似文献
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SUMMARY The analysis of records for 75 821 herd tests on 23 700 cows in the Macalister Irrigation District of East Gippsland, using a model that also included stage of lactation, herd and cow effects, found a strong relationship between somatic cell count and milk production. Unit increases in the natural logarithm of the somatic cell count were associated with decreases in production of up to 2.6% in the range 54 598 to 403 429 cells per mL. This is equivalent to a decrease of 1.9% for a doubling of cell count from 100 000 to 200 000 cells per mL. Subclinical mastitis was assessed to be a significant source of loss in milk production in the study area. 相似文献
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GA SMITH PL YOUNG† BJ RODWELL† MA KELLY† GJ STORIE† CA FARRAH† JS MATTICK† 《Australian veterinary journal》1994,71(3):65-70
SUMMARY An Australian bovine herpesvirus 1 (BHV1) isolate with a defined (427 base pair) deletion in the protein coding region of the thymidine kinase gene was obtained by standard marker rescue procedures. After selection in the presence of the nucleotide analogue 5iodo-deoxy-uridine the virus was analysed by hybridisation with three differential oligonucleotide probes, restriction endonuclease profile studies and DNA sequence analysis. The virus elicited an immune response in recipient animals after either intramuscular or intravenous administration and produced no significant deleterious side-effects when administered at a dose sufficient to stimulate the host immune response. The safety and immunogenicity of the recombinant BHV1 virus 39B1 were similar to those reported for other registered BHV1 vaccines and the virus would appear to be suitable for the production of a vaccine seed lot and more exhaustive field trials as a prelude to commercial vaccine production and registration. 相似文献
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P Rodriguez‐Villamil FL Ongaratto M Fernandez Taranco GA Bó 《Reproduction in domestic animals》2014,49(1):79-84
Three experiments were designed to test a solid‐surface vitrification system for bovine in vitro‐produced embryos and to develop a simple method of in‐straw dilution after warming, which can be potentially used for direct transfer in the field. Experiment 1 evaluated embryo survival rates (i.e. re‐expansion and hatching) after vitrification and warming in three different solutions: VS1 (20% ethylene glycol (EG) + 20% propanediol (PROH) + 0.25 m trehalose (Tr)), VS2 (20% EG + 1M Tr) or VS3 (30% EG + 0.75 m Tr). Re‐expansion and hatching rates were higher (p < 0.05) for embryos vitrified in VS3 (72.2 ± 1.9 and 58.2 ± 0.8) than VS1 (64.4 ± 0.9 and 37.2 ± 2.5) or VS2 (68.5 ± 1.5 and 49.6 ± 1.0; p < 0.05). Experiment 2 was designed to compare two methods of vitrification: glass micropipettes or solid surface, using the VS1 or VS3 solutions. No significant differences were detected between the two methods; but re‐expansion and hatching rates were higher (p < 0.05) with VS3 (73.5 ± 3.1 and 47.1 ± 2.1) than VS1 (63.3 ± 3.3 and 39.7 ± 2.8). In experiment 3, embryos were vitrified by solid surface in VS1 or VS3 solutions and cryoprotectants were diluted in‐straw after warming in a TCM 199, 0.25 m sucrose solution or holding media. Survival rates of embryos vitrified in VS3 did not differ between those exposed to 0.25 m sucrose (74.7 ± 1.3 and 57.2 ± 2.2) or holding (77.3 ± 1.4 and 58.0 ± 2.5) medium after warming; however, survival rates of embryos vitrified in VS1 were higher (p < 0.05) in those exposed to 0.25 m sucrose (67.7 ± 2.3 and 47.0 ± 1.7) than holding medium (54.5 ± 1.0 and 27.7 ± 3.1). In conclusion, solid‐surface vitrification using simplified EG‐based solutions and in‐straw dilution with holding media may be a practical alternative for cryopreservation and direct transfer of in vitro‐produced bovine embryos. 相似文献
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Cudworth DP Hegde VB Yap MC Guenthenspberger KA Hamilton CT Pechacek JT Johnson PL Bis SJ Tisdell FE Dripps JE Bruce TJ Dintenfass LP Gifford JM Karr LL Kempe MK McCormick DC Schoonover JR 《Journal of agricultural and food chemistry》2007,55(18):7517-7526
An extended lipophilic system that incorporated some key elements of first-generation 2,6-dihaloaryl actives, such as 1, demonstrated desirable efficacy against chewing insects as well as sap-feeding insects. These four-ring systems, based on 2, were accessed primarily via Suzuki couplings of halothiophene derivatives with appropriately substituted boronic acids. In particular, phenylthiophene systems that incorporated haloxyether groups, such as those in 3, 4, and 5, had the broadest spectrum of activity across chewing and sap-feeding insect pests. Expansion of this structure-activity relationship to include compounds with differing substitution patterns on the thiophene-C-ring and aryl-D-rings was undertaken. The synthesis and insecticidal activity of 3-aryl-5-(thiophen-2-yl)-1-methyl-1H-[1,2,4]triazoles will be described. 相似文献
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