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11.
Antje Semrau Gudrun Wibbelt Monika Hilbe Dietmar Lieckfeldt Robert Hermes Kerstin E Müller Hans P Heckert Mark J Hoyer Kai Fr?lich 《Journal of zoo and wildlife medicine》2008,39(1):124-127
A Lesser Malayan mousedeer (Tragulus javanicus), persistently infected with noncytopathogenic bovine viral diarrhea virus (BVDV) type 1f, was experimentally superinfected with a cytopathogenic isolate of BVDV type 1c, which antigenically partially matched the endogenous strain. Within the observational period of 125 days after superinfection, the animal did not demonstrate any clinical signs of the disease and/or significant changes in blood values. Neutralizing antibodies were detected at 35 and 42 days postinfection. The isolate causing the superinfection was found in feces, nasal swabs, and saliva starting from day 29 and at various times postchallenge. Macroscopic or histologic examination did not reveal mucosal disease-like lesions, despite the detection of the cytopathogenic isolate in the salivary gland, rumen, abomasum, kidney, and superficial prescapular lymph node. Results indicate that the cytopathogenic BVDV strain, which was used in the superinfection, persisted in the viremic animal without causing disease within the observation period. 相似文献
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Yamamoto Y Yamamoto T Yuto N Hildebrandt TB Lueders I Wibbelt G Shiina O Mouri Y Sugimura K Sakamoto S Kaewmanee S Nagaoka K Watanabe G Taya K 《The Journal of reproduction and development》2012,58(1):105-111
The objective of the present study was to define the secretion of prolactin (PRL) in pregnant African and Asian elephants. Levels of immunoreactive (ir-) PRL in serum and placental homogenates were measured by a heterologous radioimmunoassay (RIA) based on an ovine and human RIA system, and the localization of ir-PRL in the placenta was detected by immunohistochemistry using anti-human PRL. Circulating ir-PRL clearly showed a biphasic pattern during pregnancy in African and Asian elephants. Serum levels of ir-PRL started to increase from the 4 - 6th month of gestation and reached the first peak level around the 11-14th month. A second peak of circulating ir-PRL levels was observed around the 18-20th month of gestation followed by an abrupt decline after parturition. In contrast, in a case of abortion of an African elephant, the second peak of ir-PRL was not observed, and the levels remained low for about four months until parturition. The weight of the fetus delivered at the 17th month of gestation was 23.5 kg, which was quite small compared with normal fetuses in previous reports. Ir-PRL was detected in placental homogenates, and immunolocalization was observed in trophoblasts in both the African and Asian elephants, indicating that the placenta is the source of ir-PRL during pregnancy in elephants. The present results clearly demonstrated that circulating ir-PRL shows a biphasic pattern during normal pregnancy and that the placenta appears to be an important source of circulating ir-PRL during pregnancy in both African and Asian elephants. 相似文献
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Schöffmann G Vettorato E Burke JG Gibson AJ Clutton ER 《Veterinary anaesthesia and analgesia》2012,39(3):256-265
ObjectiveTo determine the effects of age, sevoflurane and isoflurane on atracurium-induced neuromuscular blockade in 3–16 week-old lambs.Study designProspective randomized experimental trial.AnimalsTwenty-six Scottish blackface ewe-lambs were anaesthetized for spinal surgery when either 3–6 (mean age 4.6 weeks; n = 18) or 12–16 weeks (mean age 13.7 weeks; n = 15) of age; seven animals were anaesthetized at both ages.MethodsAfter intramuscular injection of medetomidine (10 μg kg?1) anaesthesia was induced in the younger lambs either with isoflurane or sevoflurane in oxygen delivered by mask, and in the older lambs with ketamine (4 mg kg?1), and midazolam (0.2 mg kg?1) administered intravenously (IV). In both groups anaesthesia was maintained with fixed end-tidal concentrations of either sevoflurane (2.8%) or isoflurane (1.8%) delivered in oxygen. Before surgery meloxicam (0.6 mg kg?1), morphine (0.5 mg kg?1) and ketamine (1 mg kg?1 followed by 10 μg kg?1 minute?1) were administered IV. The lungs were ventilated mechanically to maintain normocapnia. Neuromuscular block was achieved with a loading dose (LD) of atracurium (0.5 mg kg?1 IV). The peroneal nerve was stimulated (train-of-four every 12 seconds). Evoked responses in the digital extensor muscles were evaluated by palpation and observation. Maintenance doses (MD) of atracurium (0.17 mg kg?1 IV) were administered when the first twitch (T1) returned. The onset and duration of LD action (T1 absent) and the duration of MD were recorded. Data were analysed using Student's t test, Mann–Whitney U test, repeated–measures anova, Wilcoxon's matched pairs test or Pearson correlation coefficient as relevant (p < 0.05).ResultsOnset of LD action developed significantly (p < 0.05) more rapidly in isoflurane compared with sevoflurane-anaesthetized lambs (55 ± 18 cf. 80 ± 37 seconds). Duration of action of LDs and MDs was longer (p < 0.05) in lambs aged 12–16 than 3–6 weeks (33 ± 5.4 cf. 25 ± 6.4 and 26 ± 4.2 cf. 18 ± 5.5 minutes) but were independent of the anaesthetic used.Conclusions and clinical relevanceThe effect of atracurium is age-dependent in lambs being prolonged in older animals. The onset of neuromuscular blockade is more rapid in isoflurane compared with sevoflurane-anaesthetized lambs. 相似文献
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Gudrun Overbeck 《Plant foods for human nutrition (Dordrecht, Netherlands)》1974,23(4):327-329
Ohne Zusammenfassung 相似文献
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Pasteurella are an important cause of fatal infections in free-ranging bats, but the genetic diversity of bat-derived strains is unclear. In the current study, 81 Pasteurella strains associated with pneumonia, severe organ necroses and systemic infection in free-ranging European vespertilionid bats were characterized by biochemical and molecular typing methods. Genetic relationships and subspecies status of Pasteurella multocida strains were determined by comparative 16S rDNA and rpoB gene sequence analysis. In addition, 30 representatives of the bat-derived P. multocida strains were selected based on phenotypic and genotypic tests to be compared by pulsed-field gel electrophoresis using SmaI. Most (85%) of the Pasteurella strains obtained from free-ranging bats in this study represented P. multocida ssp. septica. P. multocida ssp. multocida and Pasteurella species B were also identified in a small number of isolates. PFGE analysis correlated well with the sequencing results and revealed a high genetic diversity among bat-derived strains of P. multocida ssp. septica. Strains sharing identical or closely related SmaI fragment patterns were cultured from bats of different species, geographic origins, and years of isolation. The presence of numerous different P. multocida strains allows the assumption that Pasteurella infections in vespertilionid bats are not solely based on intra- but also on inter-species transmission. And indeed, our results present evidence of P. multocida infections in bats following cat predation. 相似文献
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Kaufhold J Hamann H Steinbach G Gordon S Brahm R Grussendorf H Rosenhagen CU Distl O 《Berliner und Münchener tier?rztliche Wochenschrift》2007,120(5-6):232-236
The inheritance of distichiasis was analyzed in the dog breed Elo using complex segregation analyses.The different hypotheses of monogenic, mixed-monogenic-polygenic and polygenic inheritance as well as a pure environmental model were tested. In further analyses the number of examined Elo dogs per ophthalmologist and the proportion of genes of Eurasian and Old English Sheepdogs were included in the models as covariates. Seven families were randomly collected for the analyses.The seven families comprised a total of 296 animals with 218 examined and 65 affected dogs. A genetic component was verified for the prevalence of distichiasis. Mixed models with a recessive, dominant or arbitrary major gene effect and the polygenic model represented likely hypotheses of inheritance. The prevalence of distichiasis observed was insufficiently explained by non-genetic factors and the breed composition. Therefore, a model only including non-genetic factors and the breed composition could be excluded. 相似文献
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Harriet Rehn Britt Berglund Jan Philipsson Ulf Emanuelson Gudrun Tengroth 《Acta Agriculturae Scandinavica, Section A - Animal Sciences》2013,63(3):137-145
The effects of grazing during summer and possible interaction effects between breed and management system on milk production and postpartum reproductive performance were studied in 93 Swedish Red and White (SRB), Swedish Holstein (SLB) and Swedish Jersey (SJB) cows with 214 initiated lactations. The cows were divided into two groups: zero-grazing with tiestalls, or grazing during summer. Effects were studied by mixed-model analysis. The results indicated that grazing (rather than zero-grazing) can reduce the milk yield in the following lactation in SLB cows, whereas it had little effect on the production of SRB cows. Grazing SJB cows produced more milk, but with a significantly lower fat content. Grazing before or during the postpartum period stimulated reproductive performance, indicating a higher conception rate in all breeds and a 13-27-day shorter interval from calving to conception in the SRB and SLB cows, whereas in the limited number of SJB cows, the trend was the converse. 相似文献