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11.
Early pregnancy diagnosis and monitoring play an important role following embryo transfer in sheep. The aims of the current study were to investigate (i) the pattern of serum progesterone profiles in sheep carrying somatic cell nuclear transfer (SCNT)‐derived (clone) pregnancies, and (ii) the frequency of pregnancy loss during development following SCNT embryo transfer. Sheep SCNT embryos were made using standard nuclear transfer techniques. Day 7 embryos were surgically transferred to oestrus‐synchronized recipients (n = 27). As a control, normal fertile ewes (n = 12) were bred by natural breeding. Serum was collected from all the ewes on the day of estrus (day 0 sample), 7 days post‐estrus (day 7 sample) and 19 days post‐estrus (day 19 sample) and every 10 days thereafter until lambing or pregnancy loss occurred. Serum progesterone (P4) was assessed using enzyme immunoassay. Pregnancy was confirmed by ultrasound scanning on day 35 of pregnancy followed by subsequent scanning every 10 days. In control ewes, pregnancy rate on day 35 was 83.3% (10/12), whereas in the ewes that received SCNT embryos, it was 22.2% (6/27; p < 0.05). The day 45 pregnancy rate in the control ewes was 83.3%, whereas in the SCNT embryo recipients it was 11.0% (p < 0.05). Hormone analysis revealed that SCNT embryo recipients exhibited a significantly lower P4 profiles at different time points in pregnancy compared to controls (p < 0.05). This study highlights the use of serum progesterone in combination with ultrasound for the investigation of embryo loss and crucial times during development of normal and SCNT embryos in sheep. Further, the serum P4 levels directly reflect the degree of placental development in these two groups.  相似文献   
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Continental extension and volcanism are generally thought to be complementary. Stratigraphic and structural data from some highly extended parts of the Basin and Range province reveal instead that rapid extension appears to have suppressed volcanism. This relation may reflect enhanced crystallization of midcrustal magmas during extension resulting from exsolution of magmatic volatiles, increased interaction of magmas with meteoric water, and dispersal of magma into smaller bodies. Some rift environments may thus be characterized by voluminous synextensional plutonism with little or no concomitant volcanism.  相似文献   
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周小舟教授认为肝癌的病因是内因正气亏虚和外因外染疫毒相互作用所致,其主要病机是气虚血瘀,针对主要病机设立芪术抗癌方辨证加减治疗,临床疗效显著;周教授通过实验及临床研究亦初步阐明了芪术抗癌方的作用机制,值得临床借鉴。  相似文献   
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为比较不同热处理条件对水牛乳稳定性的影响,实验采用不同条件热处理后的pH缓冲容量、总溶解固体(total dissolved solids,TDS)、蛋白沉淀量及表观黏度等指标研究杂交高代水牛乳样品。结果表明:与未经处理的水牛乳样品相比,水牛乳经不同条件热处理后,其pH缓冲容量、TDS、蛋白沉淀量及表观黏度皆发生显著变化。其中水牛乳分别在经121℃/15 min和95℃/5 min处理后,其pH缓冲容量、TDS、蛋白沉淀量及表观黏度分别达到最大值和最小值。因此,认为在本实验研究范围内,水牛乳经121℃/15 min及95℃/5 min处理后,对其稳定性影响最大。  相似文献   
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Data from the Global Oscillation Network Group (GONG) project and other helioseismic experiments provide a test for models of stellar interiors and for the thermodynamic and radiative properties, on which the models depend, of matter under the extreme conditions found in the sun. Current models are in agreement with the helioseismic inferences, which suggests, for example, that the disagreement between the predicted and observed fluxes of neutrinos from the sun is not caused by errors in the models. However, the GONG data reveal subtle errors in the models, such as an excess in sound speed just beneath the convection zone. These discrepancies indicate effects that have so far not been correctly accounted for; for example, it is plausible that the sound-speed differences reflect weak mixing in stellar interiors, of potential importance to the overall evolution of stars and ultimately to estimates of the age of the galaxy based on stellar evolution calculations.  相似文献   
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刈牧后休闲期对草地牧草生产的影响   总被引:2,自引:2,他引:0  
瓦庆荣  代志进  卢琪 《草业科学》2000,17(3):33-34,38
通过不同间隔时期对多年生黑麦草、白三叶混播草地牧草产量的监测与分析,得出随着休闲期的增加,青绿物质和死亡物质均呈现上升趋势,在较低和较高草地牧草现存量下都会降低牧草生长率。  相似文献   
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贵州足球场草坪建植及化管理模型研究   总被引:1,自引:0,他引:1  
在一定的生境和管理条件下,以修剪高度、修剪次数和施N、P、K量为因素,利用二次正交回归设计,用模糊综合评分法评定草坪质量,最佳3因子水平为:修剪高度5~7.4cm,修剪次数4~6次/月,施N量为2.5~3.7g/(m2·年),施P、K量各为L25~1.85g/(m2·年),建立了草坪质量与养护3因子间的显著相关回归模型.  相似文献   
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